LOCUS       c00232_gnlPro..         3045 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LAFCGIMH_232.
ACCESSION   c00232_gnlPro..
VERSION     c00232_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 00:34:14
            Original ID  :: gnl|Prokka|LAFCGIMH_232
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3045
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3045
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[39:2983]"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      40..2983
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..3045
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3045
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             40..1311
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg232_1"
                     /sec_met_domain="HGD-D (E-value: 4.5e-57, bitscore: 181.2,
                     seeds: 370, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIIETFGHYVENLTDKKPQSARRLLKTGWGAQNLKFRYMQDKRL
                     MPADRHLANLMMDTMLRPLQKPEDSVIVSIFTPCEMMQEVGLHPYNVEGFSCYLSASKA
                     ERAFLQQAENQGIAETLCSYHKTFLGAAQKGLLPKPKCIVYTNLTCDANLLTFRTLADF
                     YQVPVFAIDVPWNQTTENVQYVADQLKDLKIFLEKNTGKTISEDRLKERLACSKRTLEN
                     YKKYQQMRADRYVPSDLVTPLYAGMTNNILLGTAEEEKYTQMLLEDIKKAPAAKGKHIY
                     WMHTIPFWSDAVRQELCFSEKAQIVGCELAETCEPDFDPENPFEAMAERMVYHSLNGSA
                     LRRIEAGIRHAKQAGADGAVWFGHWGCKHTLGAAQLAKKKFEEAGLPLLILDGDGCDRS
                     HGGEGQTSTRLGAFLEMLGGAENE"
     CDS             1304..2983
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg232_2"
                     /sec_met_domain="HGD-D (E-value: 2.7e-18, bitscore: 53.8,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 1.9e-66, bitscore:
                     211.4, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTYYVCKYTPIELLESLGGECENFNHMPEGFDLADQVSHPNIC
                     GFGKTLLEGVMEGKIKELVLVSCCDTIRSVYDILLESGKMDFLYILDVLHCDSACSRER
                     MAVQLKGLAKAYAQYKGTEFDAGKFRAAFHAQEKITKSHIAVLGARMGQELFEMTSKAM
                     PLPVENDTCVHNRSVGNILPPEGASFDELMDWYAGEILGQIPCMRMMDPTGRKKLYNDP
                     SVAGIIYHTVKFCDFYSFEYAEIKNHTDVPLLKIESDYTIQSSGQLLTRLEAFAESIQP
                     ETLEQTIDGDTKGERKMGKGYFAGIDSGSTSTDVVILNKDHEIVTSIILPTGAGAAIGA
                     DRALAEALKEAGLQREDIDALVTTGYGRTAIKNGDKSITEITCHARGAHFLNPEVRTVI
                     DIGGQDSKVIRLDENGAVANFVMNDKCAAGTGRFLEMMARTMEMNLDQMSECGLEFKED
                     ITISSMCTVFAESEVVSLIAQNKATDDIVHGLNKAVAVKTAALTRRVGGEEKYMMTGGV
                     SKNKGLVKTLEEKLGTKLVISDKAQLCGALGAALFAADMADV"
ORIGIN
        1 tgataaatta ttattaccat aatgaggaag agacagacta tggagattat tgagacattt
       61 ggtcattatg tggaaaatct gacggataaa aagcctcagt cagcccgccg gctgttaaaa
      121 accggttggg gggcgcagaa tctgaagttc cgttacatgc aggacaagag actgatgcct
      181 gcagacaggc atcttgcaaa tctgatgatg gataccatgc tgcggcccct tcagaaaccg
      241 gaagattctg ttattgtaag tatttttaca ccctgcgaaa tgatgcagga ggtgggatta
      301 catccctata atgtagaagg attttcctgc tatctttctg caagcaaagc agagagggca
      361 tttttgcagc aggcagagaa tcagggaatt gcagagacac tgtgcagtta tcacaagaca
      421 tttcttggtg cagcgcagaa aggacttctg ccaaagccta aatgtattgt ttataccaac
      481 ctgacctgcg atgctaacct gctcaccttc cgtaccctgg cagattttta ccaggtacct
      541 gtatttgcca ttgatgtacc atggaatcag accacagaga atgtacagta tgtggcagat
      601 cagttaaagg atttaaaaat atttctggaa aaaaatacag gaaaaacaat ctctgaggac
      661 agattaaagg aacgtcttgc ctgcagtaag cggactctgg agaattacaa aaaatatcag
      721 cagatgcgtg cagacagata tgtgccctct gatctggtca cacccttgta tgcaggaatg
      781 acaaacaata tccttctggg aacagcagaa gaagagaagt atacacagat gcttcttgaa
      841 gatattaaga aagcaccggc agcgaaaggg aagcacattt actggatgca taccattcca
      901 ttctggtcgg atgcagtgcg ccaggagctt tgcttcagtg agaaagccca gatcgtagga
      961 tgtgaacttg cagaaacctg tgagccggat tttgatcctg agaacccctt tgaagcaatg
     1021 gcagaaagaa tggtatatca ttcactgaac ggaagtgccc ttagaaggat tgaagccggt
     1081 atccgccatg cgaaacaggc aggagcagac ggtgctgtgt ggtttggaca ttggggatgt
     1141 aaacatacgc ttggtgcggc acagcttgca aagaagaagt tcgaggaagc cggtctgccc
     1201 cttttgatcc tggacggtga cgggtgtgat cgaagccatg gaggagaagg acagacttcc
     1261 accaggctgg gagcttttct ggaaatgctg ggaggtgctg agaatgaata aaacttatta
     1321 tgtctgtaaa tataccccaa ttgaacttct ggaatctctt ggcggggaat gtgagaactt
     1381 taatcatatg ccggagggat ttgacctggc cgatcaggtt tcacatccca atatctgcgg
     1441 ttttggaaaa acacttctgg aaggagtcat ggaggggaaa atcaaggaac ttgtcctggt
     1501 gagctgctgt gatactatcc ggagtgtcta tgacattctg cttgaaagcg gcaagatgga
     1561 ttttctctat attttggatg tgcttcactg tgacagtgcc tgtagcagag agcgtatggc
     1621 tgtacagtta aaagggctgg cgaaggctta tgcacagtac aaaggcacag agtttgatgc
     1681 cggaaaattc cgggctgctt tccatgcaca ggagaaaatc acaaagtccc atattgcagt
     1741 gcttggcgcc agaatggggc aggaattatt tgaaatgacc agcaaggcta tgcctcttcc
     1801 tgtagagaat gatacctgcg tgcataaccg ttctgtggga aatatacttc caccggaggg
     1861 tgcttctttt gatgagctga tggactggta tgcaggtgag attctggggc agattccatg
     1921 tatgcgtatg atggacccga caggtcgtaa gaagctttat aatgatccgt ctgttgcggg
     1981 gattatttat catacagtga agttttgtga tttttacagc ttcgaatatg ctgagattaa
     2041 gaatcataca gatgtacctc ttttaaaaat cgaatcagat tatactattc agagcagcgg
     2101 tcagcttctt accagactgg aagcttttgc agagagcatt cagcctgaaa cactggagca
     2161 gaccatagat ggagacacaa aaggagagag aaaaatggga aaaggttatt tcgcagggat
     2221 tgacagtggt tccaccagta cagatgtggt gattttaaat aaagatcatg agattgtgac
     2281 cagcattatc ctgcctacag gagcaggagc tgctatcgga gcagaccgtg cccttgcaga
     2341 agcattgaag gaggccggat tgcagagaga agatattgat gcgctggtta ctaccggata
     2401 cggacgtaca gctattaaaa atggtgataa gagtatcaca gagatcacct gtcatgcaag
     2461 gggtgcgcat ttcctgaatc cggaagtccg tactgttatt gatattggcg gacaggacag
     2521 caaggtgatc cgactggatg agaacggtgc agttgcaaac tttgttatga atgacaaatg
     2581 tgcggcagga accggacgat ttctggaaat gatggcacgt accatggaaa tgaatctaga
     2641 tcagatgagc gaatgtgggc tggaattcaa agaagatatt acaatttcca gtatgtgtac
     2701 agtatttgca gaatcagaag ttgtatcact gatcgcccag aataaggcaa cagatgatat
     2761 tgtccatggc ctgaataagg cggttgccgt aaagactgcc gcccttacca gacgtgtggg
     2821 cggtgaggag aaatatatga tgaccggtgg tgtttctaag aataagggtc tggtaaagac
     2881 actggaagag aagcttggaa caaagctggt gatcagtgac aaggctcagc tttgcggtgc
     2941 acttggcgca gcactgtttg cggctgatat ggctgatgtg taaattttta gttactgttc
     3001 actccgttct cagcaacacg cttcgcgatg caggaaatat gaacc
//
