LOCUS       c00177_gnlPro..         4839 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BAEMBNGD_177.
ACCESSION   c00177_gnlPro..
VERSION     c00177_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 00:32:50
            Original ID  :: gnl|Prokka|BAEMBNGD_177
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4839
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4839
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[46:4837]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      47..4837
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..4839
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4839
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(47..1459)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: HMGL-like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: PYC_OADA"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg177_1"
                     /sec_met_domain="HMGL-like (E-value: 3.6e-44, bitscore:
                     139.9, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="PYC_OADA (E-value: 1.9e-59, bitscore:
                     189.5, seeds: 434, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEIKKKPVKIVETVLRDAHQSLIATRMTTEEMLPIVDKMDQVGY
                     HAVECWGGATFDACLRFLKEDPWERLRKLRDGFKNTKLQMLFRGQNILGYKPYPDDVVE
                     YFVQKSIANGIDVIRIFDCLNDLRNLQTAVDATKKEGGHAQIALSYTIGDAYTLDYWKK
                     TAKNIEEMGADSICIKDMAGLLVPNEATRLVTALKQATDIPIELHTHYTSGVGGMTYLK
                     AVEAGVDIIDTAISPFAMGTSQPATEVMAETFRGTEFDTGFDQKLLGEIADYFRPIREK
                     YIANGLMSPKVLGVNIKTLMYQVPGGMLSNLVSQLEQQGASDKFYEVLEEVPKVRKDFG
                     EPPLVTPSSQIVGTQAVLNVLTGERYKMITNESKALLRGEYGQTVKPMNKEVQKKAIGD
                     EKPITCRPADLLEPELSKIEAEMSQWKEQDEDVLTYALFPQVATEFFKYRAAQENKIDP
                     AKADVKNGAYPV"
     CDS             complement(1617..2741)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg177_2"
                     /sec_met_domain="OAD_beta (E-value: 5.5e-158, bitscore:
                     514.1, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYVVETMTNLAAQTGFAFLKPGNFIMILVALVFLYLAIAKGYEP
                     LLLVPISFGMLLVNLYPSIMEEGGLLHYFYLLDEWSILPSLIFLGVGALTDFGPLIANP
                     ASFLLGAAAQFGIYSAYFLAMLMGFNGKAAAAISIIGGADGPTSIFLAGKLGQTDLMGP
                     IAVAAYSYMSLVPIIQPPIMKLLTTEKERQIKMEQLRTVTKTEKIMFPIVVTVVVCLIL
                     PTTAPLVGMLMLGNLFRESGVVKQLSETASNALMYIVVIILGTSVGATTSAEAFLNVST
                     IKIVVLGLVAFAVGTAAGVLFGKLMCKVTGGKVNPLIGSAGVSAVPMAARVSQKVGAEA
                     DPSNFLLMHAMGPNVAGVIGTAVAAGTFMAIFGV"
     CDS             complement(2775..3143)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 66.6; E-value:
                     4.3e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg177_3"
                     /sec_met_domain="Biotin_lipoyl (E-value: 4.2e-22, bitscore:
                     66.6, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNYRITVNGVAYDVAVEELGAGEASAAPVSTPAAVPAPAKKAAP
                     KTSGSAGAVKITAPMPGKIVAVKAQAGASVKKGDAVLVLEAMKMENEICAAQDGVIASV
                     EVAVGDMVEGGDVLATMN"
     CDS             complement(3238..3582)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg177_4"
                     /sec_met_domain="OAD_gamma (E-value: 2.7e-28, bitscore:
                     87.2, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVFEQALLNTLMGMGTVFAVLIFISLLISLFVYIPSIERALKNR
                     SSKKEKKAAQEERPAPKRPILEEVVEEEELVDDGELVAVITAAIMAANGGAAVSADKLV
                     VRSIKRVKRR"
     CDS             complement(3595..4050)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg177_5"
                     /sec_met_domain="CT_Vparvula (E-value: 1.4e-36, bitscore:
                     114.8, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VTGLRANAHEERKQALALAKFTTTLAQVSVPRINLIAGEAYGTAA
                     EVMNSKAIGADFVLAWPQASVGMMDAEQAVRIMYAEEINTDKNKTALIAEKTAEYAEGQ
                     SSALAAAKRGYIDDIIEPDATRKRLIAAYEMLYGKKVTPVTKKHTAV"
     CDS             complement(4160..4837)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg177_6"
                     /sec_met_domain="CT_Vparvula (E-value: 1e-55, bitscore:
                     177.9, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="QDASALGGSVGEMHAKKIASLYDLAMKTGAPVIGMLDCAGLRLQE
                     ATDALHAFGTIFNKQAQASGMIPQISAVFGMCGGGSAVMSAMSDFTLMEKENGSLFINS
                     PNALAGNEESRQNTAGAQFQSEQAGNVDFVCETEEGLLDQLRILVDVLPSDYEAEDVYF
                     DNDDDLNRVIPELNDAAYDAAYILKSISDNNMYIETKAAYAKEMVXXXXIYPSEWRDSR
                     CGC"
ORIGIN
        1 taaagttgcg ggggttcgtc tgtttttaat ctaaatcaat ccaatcctac accggatatg
       61 ctccattctt tacatcggct tttgcaggat caatcttatt ctcctgtgcc gctctgtatt
      121 taaagaattc ggttgctacc tgtgggaaca atgcgtatgt aagcacatct tcgtcctgtt
      181 ctttccactg gctcatctct gcctcgatct tagataattc cggttcaaga aggtcggctg
      241 gacgacaggt aattggcttt tcatcgccga ttgccttttt ctgtacttct ttattcattg
      301 gttttacggt ttgaccgtat tctccacgaa gaagggcttt tgactcgttt gtaatcattt
      361 tataacgctc tccggtaagt acatttaata cagcctgtgt tccgacaatc tgactggacg
      421 gtgtaactaa tggtggttca ccgaagtctt tacgtacttt tggaacttcc tctaatactt
      481 catagaactt atcggatgct ccctgctgct ctaactgaga tacaaggtta gaaagcattc
      541 cacctggaac ctgatacatt aaagtcttga tgtttacacc aagtaccttt ggactcatta
      601 agccatttgc aatgtatttc tcacggatcg gacggaagta atctgcgatt tcacctaaaa
      661 gcttctggtc aaatcctgtg tcaaattctg taccacggaa tgtctccgcc ataacttctg
      721 ttgccggctg gcttgttccc attgcaaatg gagatatggc tgtatcaatg atatcgactc
      781 ctgcttctac tgcttttaag taagtcattc caccgacacc acttgtatag tgtgtatgaa
      841 gttcgatagg aatgtctgtt gcctgcttta atgcagttac aaggcgagtt gcttcattcg
      901 gcactaaaag tcctgccata tctttgatac agatagaatc tgctcccatc tcttcaatat
      961 tcttagcagt ctttttccag tagtctaatg tatatgcatc accgattgta taggagaggg
     1021 cgatctgcgc gtgtcctccc tctttctttg ttgcatctac agcagtctgt aagtttctaa
     1081 ggtcatttaa acagtcaaaa atacgaataa catcaattcc gtttgcaata gatttctgta
     1141 cgaaatattc tacaacatcg tctggatatg gcttatatcc taagatgttc tgtccacgga
     1201 ataacatctg taactttgta ttcttaaagc catctcttaa tttacgaagt ctctcccatg
     1261 ggtcttcctt taagaaacgc aggcaggcat caaatgttgc acctccccag cattctactg
     1321 catgataacc aacctgatcc atcttatcta cgattggcag catctcctct gttgtcattc
     1381 ttgtagcaat cagagactga tgtgcgtcac ggagaacggt ttctactatt ttaaccggtt
     1441 ttttcttaat ttcggccata atatctcctt ttatttcaaa tcacgtaact atttgcgctt
     1501 acaactgtta ttatctaaaa ttcaacttgt aaactattta taaatatccc ctgtaacatt
     1561 ttttacagat tgtagcccta taaataatga attttaaata atcaaataat tctaaattat
     1621 actccaaaaa ttgccataaa tgttccggcg gctaccgctg taccgataac tccggcaaca
     1681 tttggtccca tcgcatgcat cagtaagaag tttgatggat ctgcttccgc accaactttc
     1741 tgggatacac gggctgccat aggaacggca gataccccgg cagaaccaat taatggattt
     1801 accttaccac ctgtaacctt acacataagt ttaccaaaca ggacacctgc tgcagtacca
     1861 acagcaaaag ctacaagacc taatactaca atcttgattg tgctgacatt taagaatgcc
     1921 tctgcacttg ttgtcgcacc aacggatgta ccaagaataa taactacgat gtacataaga
     1981 gcgttagacg ctgtctctga aagctgtttt actacgccgg actctctaaa aaggttaccg
     2041 agcatcagca taccaactaa tggtgctgtt gtaggaagaa tcagacatac aacaactgtt
     2101 acaacgatag ggaacataat tttctctgtt tttgtaacgg ttcttaactg ttccatctta
     2161 atctgacgtt ccttttctgt agtaaggagc ttcatgatcg gtggctgaat aattggtaca
     2221 agtgacatat aagaatatgc cgctaccgca attggtccca ttaagtctgt ctgcccaagc
     2281 tttccggcaa ggaaaataga agtaggacca tcggctccac caataatgga aattgctgct
     2341 gctgcctttc cgttaaatcc cataagcatt gcaaggaaat acgcgctgta aataccaaac
     2401 tgcgccgcag ctcccagcaa gaaacttgct ggattggcaa tcagcggacc aaagtctgtt
     2461 agtgctccaa cacctaagaa aatcagtgaa gggagaatac tccattcatc taataagtaa
     2521 aaataatgta ataatccgcc ttcttccata atggacggat ataagttaac cagcagcata
     2581 ccaaaagaaa tcggcactaa caaaagtggc tcatatcctt tggcgatggc cagatataag
     2641 aagactaaag caaccagtat cataatgaag ttacctggct ttaagaatgc aaatccggtc
     2701 tgtgctgcga gattcgtcat tgtctctaca acataactca taaatcttcc tccgttttat
     2761 tttgcaaata tgctctagtt cattgtagcc agtacatctc cgccttctac catatctcca
     2821 actgctactt ccacacttgc gataacacca tcctgtgctg cacagatttc attctccatc
     2881 ttcatagcct ctaatacaag aacagcgtcg cccttcttta cagaagcacc tgcctgtgcc
     2941 tttactgcta cgatcttacc tggcattggt gctgtaatct ttacggcacc ggcacttccg
     3001 gaagtctttg gggcagcttt cttagctgga gccggaacgg ctgctggtgt acttaccggc
     3061 gctgcacttg cttctcctgc acctaattct tctactgcta catcataagc aacaccatta
     3121 actgtaattc tataattttt cattttaaat cctcctgaat gattttatgt acttctaaaa
     3181 tctttctata gctaactttt caaaacttta taaaaagatt ccaagaatac atttttgcta
     3241 tcttctcttc actctcttga ttgaacgaac gaccaactta tctgcactga ctgctgctcc
     3301 tccattcgct gccatgattg ccgctgtgat tactgcgaca agttcaccat catccacaag
     3361 ttcctcttct tccacaactt cttcaagaat cggacgcttt ggagccggtc tttcttcctg
     3421 cgcagccttt ttctctttct tagaagaacg attctttaac gctctttcaa tagatgggat
     3481 atatacaaaa agagaaatca gtaaagaaat gaaaatcagt actgcaaata cagttcccat
     3541 acccatcaaa gtatttaata aggcttgttc aaatacgttc atactctcac ctcactatac
     3601 tgccgtatgc ttctttgtca ctggagttac tttctttccg taaagcattt cataagcagc
     3661 gataagacgc tttcttgttg cgtccggctc gatgatgtca tctatatatc ctctctttgc
     3721 agctgctaat gcactgctct gtccttctgc atattccgca gttttctctg cgataagtgc
     3781 tgtcttattc ttatctgtat tgatttcctc tgcatacatg atacgaactg cctgttctgc
     3841 atccatcatt cctacagatg cctgtggcca tgccagaaca aagtcagctc cgatagcttt
     3901 agaattcatt acttccgctg cagttccata agcctcgcct gcaatgagat taatacgagg
     3961 tacactaacc tgtgcaagtg ttgttgtaaa cttcgctaat gcgagcgcct gctttctctc
     4021 ttcatgtgcg ttggcacgaa gtcctgtcac gttcgttaca gtaagaagag gaatcgaaaa
     4081 tgcatcacag aacttaataa atctttctgc tttttcaaga ccaattcttg ttaaaacgga
     4141 ctcaccatct actggctggt tagcaaccgc acctactgtc tctccattca gacggataaa
     4201 tgnnnnnnnn nntcaccatc tctttggcat aggctgcctt tgtctctatg tacatattgt
     4261 tgtcagaaat agattttaaa atgtaagcgg catcatatgc ggcatcattt aactcaggaa
     4321 taaccctatt taaatcatca tcgttatcaa agtaaacatc ttctgcttca taatcagaag
     4381 gaagtacatc aacgagaata cgaagctggt caagaagtcc ttcttctgtc tcacatacaa
     4441 aatctacgtt accagcctgc tcagactgga actgtgctcc tgccgtattc tgacgggatt
     4501 cttcattccc cgctaatgca ttcggactgt tgataaagag actgccgttc tccttctcca
     4561 tcaatgtaaa atcagacata gcggacatta cggcacttcc tcctccacac attccaaata
     4621 ctgcagaaat ctgtggaatc attccagatg cctgtgcctg cttattaaaa atcgttccaa
     4681 atgcgtgaag ggcatctgtc gcttcctgta atctaagtcc tgcacaatcc agcattccaa
     4741 tgactggcgc gcctgttttc atcgccagat cataaaggga agcaattttc ttcgcatgca
     4801 tctcacctac ggaacctcca agtgcggaag catcctgac
//
