LOCUS       c00284_gnlPro..         3657 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KIJAEOBI_284.
ACCESSION   c00284_gnlPro..
VERSION     c00284_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 04:45:17
            Original ID  :: gnl|Prokka|KIJAEOBI_284
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3657
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3657
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:1916]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..1916
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3657
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3657
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..1181
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg284_1"
                     /sec_met_domain="Gly_radical (E-value: 7.3e-47, bitscore:
                     146.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.4e-118, bitscore:
                     384.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRS
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREMQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             1182..1916
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg284_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.1e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             2114..2692
                     /locus_tag="ctg284_3"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPDADLDSLASIIESSKAAKGELLLDNG
                     QISKYLYYVESGLLRQFYYKNKRDITEHFSCEGNIVICIASTFQREPMRLLIEALEPTV
                     YHRISFSEFSDLLKTSLAINRLYQKILEWALMLSQEKADSWRFESARKRYLRFQQEYPE
                     AAKRASVNHIASYLLMTPESLSRVRAGVL"
     CDS             2800..3657
                     /locus_tag="ctg284_4"
                     /transl_table=11
                     /translation="MKIEKITGREILDSRGNPTVEVDILLESGVMGRASVPSGASTGEN
                     EALELRDGDKKRYGGKGVLKAVENINTIIAPALKGMSALDQIGIDHAMLALDGTKTKSK
                     LGANAILGVSLAVAKAAANYLDIPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIAFQE
                     FMIRPVGAASFKEGLRMGAEVFHALKKVLHDRGLSTAVGDEGGFAPNLEGTEDALNSII
                     AAIKAAGYEPGKDVMIGMDCASSEFYKDGIYDYTKFEGEKGVKRTSDEQVDYLEKLINE
                     YPIDS"
ORIGIN
        1 atttaggtcc ggcaccggaa cccaacttga cggtgctgtg gttcaagaac gctcccgaga
       61 actggaaacg tttctgtgcc aaggtctcta tcgatacgtc ggctatccaa tatgagaatg
      121 acgatttgat gcgtccggat tatggggatg attatggtat cgcttgttgc gtttccccca
      181 tgaagatcgg aaagcagatg cagttcttcg gcgctcgtgc gaatttagcg aagtgtttat
      241 tgtatgcgat caatggagga cgggatgagc gtagcggcgt acaggtagcc ccgatgttcg
      301 aaccggttcg cagtgagtat ctggagtatg atgaggtgat ggctaagtat gaacaaatga
      361 tgcgctggtt ggcgaaggta tatgtgaacg ccttgaagat aatccattat atgcatgata
      421 aatatgcgta tgaggcattc gagatgtcgc ttcacgatgg ggatgtagag cgtatccgtg
      481 cgacaggtat cgccggtctt tctatcgttg ccgattcgtt ggccgctatc cgtgatacga
      541 aagtacgtgt gatccgtgac gagcgtggct tggcggttga tttcgaacgt gagggcgaat
      601 atgttccttt cggtaataac gatgatcgta cggatagtat cgctgtagag atcaccgaga
      661 agttcatgga atatttgcgt cagcacgaga cctatcgcca tgcgaaacct acgcagtcca
      721 tccttacgat cacctctaat gtggtatatg gtaagaagac tggtacgaca ccggatggcc
      781 gtatcggtgg aactcctttc gctccgggcg cgaaccctat gaacggtcgt gatacgaaag
      841 gtgctatcgc tgcgctagct tcggtagcga agcttccatt ccagcatgcg cacgacggca
      901 tctcttatac tttcgctgtt tctccggcta cgttgggaaa ggagcgtgaa atgcagatca
      961 ataatctggt tagcttattg gatggctatt ttactccgga tggaggtcag catctgaatg
     1021 ttaacgtatt tgataaggat ttgttgatcg acgctatgga acatcccgag aaatatccgc
     1081 aacttacgat ccgtgtatcc ggatatgcgg taaacttcgt gaaactgact cgtgaacaac
     1141 agttggatgt tatttcaaga accattaacc attcactgta agtgataaaa ggtaagatac
     1201 attcattaga gagtttcggg accgtagatg gtcccggaat tcgctttgtc gtttttatgc
     1261 agggctgccc gttgcgttgc ctgtattgcc ataacccgga tacttggaac ccgaaaggga
     1321 aggtgaaata tcagatgact cccggcgagc tattgacgga agttttacgg tataagagtt
     1381 ttatcgcccg tgggggggtg actgttaccg gtggggaacc tttattgcaa cctgagtttc
     1441 tgaaagagtt tttccgtttg tgccaagagc aaggtcttca cacagctttg gacacatctg
     1501 gtttcgtatg tacctcaaaa gcttgggaag tcttggatta tgcggactta gtgttattgg
     1561 acatcaaaac cttaaacccg gatttacatc cgttactggc gggtgtcaag caggataata
     1621 cgcttctgtt cttggatgaa ttggagagac gtggcataga tacttggatt cgccatgtta
     1681 ttgtccccgg atatacggat aatgacgagt ggctggaggc gttagctcgg tatgtcagtt
     1741 catataaagt ggttcgcaaa gtggaattat tgccttatca tacgatggga acctataaat
     1801 atgagcaatt aggcctcgat tatccgttaa agggggtgga acccttatca aaagaacgtt
     1861 tagataatgc gaaagcgata ttctctcgct acaaaccaga aaataacaag gcctgatctc
     1921 tcgatcaagc cttgcgatta tgcaacagct aatcagggaa aacaatctaa tccgattagc
     1981 ttttaaacga tagtaataat ttttgcctag actaaccttg actatttaat atgtgtttgt
     2041 tttgttatat gcaacaaata tctatttttg taaggagata aaaaatgata tagatcagaa
     2101 aatgatgaat agtatgaaga caccacaaga aatagccgaa atgatatcca aacgttttta
     2161 tccattaccc gatgcggatt tggattctct ggcctcgatc atagaatcaa gtaaggctgc
     2221 gaaaggagaa ttattgttgg ataacggaca aatatctaaa tatttatatt atgtggaatc
     2281 gggattatta cgccagtttt actataagaa taaacgtgat attaccgagc atttctcctg
     2341 tgagggaaat atcgttattt gtatagccag cacattccaa agagaaccga tgcgtttatt
     2401 aatcgaggca ttggaaccga cggtttatca ccggatctct ttttcggaat ttagcgattt
     2461 attgaaaaca tctttagcta ttaatagatt gtaccagaaa atactggagt gggctttgat
     2521 gctttcgcaa gagaaagcgg actcttggcg ttttgagtcg gcaagaaaac gttatctgcg
     2581 ttttcaacag gaatatccgg aagcagcgaa acgtgcttct gtcaatcata tcgcctcgta
     2641 tttattgatg acacccgaat ctttgagccg ggtgcgggcg ggagtgttgt agtctctacc
     2701 tacattcagg tattattgag gtgaaaatag caatttgtgg tgatttcacg gttcaagtaa
     2761 tccatctata tttgcgtcag tttaaaaaac gtagaagcaa tgaaaataga aaagattaca
     2821 ggacgtgaga tcctagattc aagaggtaac ccgacagtag aggttgatat tttactagag
     2881 tccggcgtta tgggccgtgc gtctgtgcct tccggtgcgt ccaccggcga gaacgaggct
     2941 ttggagttgc gtgatggcga caagaagcgt tatggtggta aaggcgtatt gaaagcggtt
     3001 gagaatatca atacgattat cgctccggca ttgaagggga tgtctgcctt ggatcagata
     3061 ggtatcgatc atgcgatgtt ggctttggac ggtacgaaaa ctaaatcgaa actaggtgcg
     3121 aacgctatcc ttggcgtttc tttagctgta gcgaaagctg ccgcaaatta tttggatatt
     3181 cctttatata gatatattgg tggaacgaat acctacgtga tgcctgttcc gatgatgaat
     3241 ataatcaatg gcggttcgca tagcgacgct ccgatcgctt tccaagagtt tatgattcgt
     3301 ccggtaggtg ccgcttcttt caaggaaggc ttacgtatgg gcgctgaggt attccatgcg
     3361 ttgaaaaagg tattgcacga tcgtgggtta agtaccgctg ttggtgatga gggtggtttc
     3421 gccccgaatt tggaaggtac ggaagatgcc ttgaactcaa ttatcgccgc tatcaaggcg
     3481 gctggttatg aaccgggcaa ggatgtgatg atcggtatgg attgcgcttc ttctgagttt
     3541 tataaggatg gaatctacga ttacacgaag tttgaaggag aaaaaggagt gaaacgtact
     3601 tccgacgaac aagtggatta tctggaaaag ctgatcaacg aatacccgat cgattcc
//
