GCA_934883025.1 - 4 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaEPKABKHL_21 (original name was: gnl|Prokka|EPKABKHL_21)

Region	Type	From	To	Most similar known cluster		Similarity
Region 21.1	ranthipeptide	2,723	24,311

gnlProkkaEPKABKHL_23 (original name was: gnl|Prokka|EPKABKHL_23)

Region	Type	From	To	Most similar known cluster		Similarity
Region 23.1	NRPS	1	39,960	dipeptide aldehydes	NRP	47%

gnlProkkaEPKABKHL_29 (original name was: gnl|Prokka|EPKABKHL_29)

Region	Type	From	To	Most similar known cluster		Similarity
Region 29.1	lanthipeptide-class-ii	19,302	41,932

gnlProkkaEPKABKHL_33 (original name was: gnl|Prokka|EPKABKHL_33)

Region	Type	From	To	Most similar known cluster		Similarity
Region 33.1	cyclic-lactone-autoinducer	21,874	37,295

Region	Type	From	To	Most similar known cluster	Similarity
Region 21.1	ranthipeptide	2,723	24,311
Region 23.1	NRPS	1	39,960	dipeptide aldehydes	NRP	47%
Region 29.1	lanthipeptide-class-ii	19,302	41,932
Region 33.1	cyclic-lactone-autoinducer	21,874	37,295

No secondary metabolite regions were found in these records:: gnlProkkaEPKABKHL_1 (original name was: gnl|Prokka|EPKABKHL_1); gnlProkkaEPKABKHL_2 (original name was: gnl|Prokka|EPKABKHL_2); gnlProkkaEPKABKHL_3 (original name was: gnl|Prokka|EPKABKHL_3); gnlProkkaEPKABKHL_4 (original name was: gnl|Prokka|EPKABKHL_4); gnlProkkaEPKABKHL_5 (original name was: gnl|Prokka|EPKABKHL_5); gnlProkkaEPKABKHL_6 (original name was: gnl|Prokka|EPKABKHL_6); gnlProkkaEPKABKHL_7 (original name was: gnl|Prokka|EPKABKHL_7); gnlProkkaEPKABKHL_8 (original name was: gnl|Prokka|EPKABKHL_8); gnlProkkaEPKABKHL_9 (original name was: gnl|Prokka|EPKABKHL_9); gnlProkkaEPKABKHL_10 (original name was: gnl|Prokka|EPKABKHL_10); gnlProkkaEPKABKHL_11 (original name was: gnl|Prokka|EPKABKHL_11); gnlProkkaEPKABKHL_12 (original name was: gnl|Prokka|EPKABKHL_12); gnlProkkaEPKABKHL_13 (original name was: gnl|Prokka|EPKABKHL_13); gnlProkkaEPKABKHL_14 (original name was: gnl|Prokka|EPKABKHL_14); gnlProkkaEPKABKHL_15 (original name was: gnl|Prokka|EPKABKHL_15); gnlProkkaEPKABKHL_16 (original name was: gnl|Prokka|EPKABKHL_16); gnlProkkaEPKABKHL_17 (original name was: gnl|Prokka|EPKABKHL_17); gnlProkkaEPKABKHL_18 (original name was: gnl|Prokka|EPKABKHL_18); gnlProkkaEPKABKHL_19 (original name was: gnl|Prokka|EPKABKHL_19); gnlProkkaEPKABKHL_20 (original name was: gnl|Prokka|EPKABKHL_20); gnlProkkaEPKABKHL_22 (original name was: gnl|Prokka|EPKABKHL_22); gnlProkkaEPKABKHL_24 (original name was: gnl|Prokka|EPKABKHL_24); gnlProkkaEPKABKHL_25 (original name was: gnl|Prokka|EPKABKHL_25); gnlProkkaEPKABKHL_26 (original name was: gnl|Prokka|EPKABKHL_26); gnlProkkaEPKABKHL_27 (original name was: gnl|Prokka|EPKABKHL_27); gnlProkkaEPKABKHL_28 (original name was: gnl|Prokka|EPKABKHL_28); gnlProkkaEPKABKHL_30 (original name was: gnl|Prokka|EPKABKHL_30); gnlProkkaEPKABKHL_31 (original name was: gnl|Prokka|EPKABKHL_31); gnlProkkaEPKABKHL_32 (original name was: gnl|Prokka|EPKABKHL_32); gnlProkkaEPKABKHL_34 (original name was: gnl|Prokka|EPKABKHL_34); gnlProkkaEPKABKHL_35 (original name was: gnl|Prokka|EPKABKHL_35); gnlProkkaEPKABKHL_36 (original name was: gnl|Prokka|EPKABKHL_36); gnlProkkaEPKABKHL_37 (original name was: gnl|Prokka|EPKABKHL_37); gnlProkkaEPKABKHL_38 (original name was: gnl|Prokka|EPKABKHL_38); gnlProkkaEPKABKHL_39 (original name was: gnl|Prokka|EPKABKHL_39); gnlProkkaEPKABKHL_40 (original name was: gnl|Prokka|EPKABKHL_40); gnlProkkaEPKABKHL_41 (original name was: gnl|Prokka|EPKABKHL_41); gnlProkkaEPKABKHL_42 (original name was: gnl|Prokka|EPKABKHL_42); gnlProkkaEPKABKHL_43 (original name was: gnl|Prokka|EPKABKHL_43); gnlProkkaEPKABKHL_44 (original name was: gnl|Prokka|EPKABKHL_44); gnlProkkaEPKABKHL_45 (original name was: gnl|Prokka|EPKABKHL_45); gnlProkkaEPKABKHL_46 (original name was: gnl|Prokka|EPKABKHL_46); gnlProkkaEPKABKHL_47 (original name was: gnl|Prokka|EPKABKHL_47); gnlProkkaEPKABKHL_48 (original name was: gnl|Prokka|EPKABKHL_48); gnlProkkaEPKABKHL_49 (original name was: gnl|Prokka|EPKABKHL_49); gnlProkkaEPKABKHL_50 (original name was: gnl|Prokka|EPKABKHL_50); gnlProkkaEPKABKHL_51 (original name was: gnl|Prokka|EPKABKHL_51); gnlProkkaEPKABKHL_52 (original name was: gnl|Prokka|EPKABKHL_52); gnlProkkaEPKABKHL_53 (original name was: gnl|Prokka|EPKABKHL_53); gnlProkkaEPKABKHL_54 (original name was: gnl|Prokka|EPKABKHL_54); gnlProkkaEPKABKHL_55 (original name was: gnl|Prokka|EPKABKHL_55); gnlProkkaEPKABKHL_56 (original name was: gnl|Prokka|EPKABKHL_56); gnlProkkaEPKABKHL_57 (original name was: gnl|Prokka|EPKABKHL_57); gnlProkkaEPKABKHL_58 (original name was: gnl|Prokka|EPKABKHL_58); gnlProkkaEPKABKHL_59 (original name was: gnl|Prokka|EPKABKHL_59); gnlProkkaEPKABKHL_60 (original name was: gnl|Prokka|EPKABKHL_60); gnlProkkaEPKABKHL_61 (original name was: gnl|Prokka|EPKABKHL_61); gnlProkkaEPKABKHL_62 (original name was: gnl|Prokka|EPKABKHL_62); gnlProkkaEPKABKHL_63 (original name was: gnl|Prokka|EPKABKHL_63); gnlProkkaEPKABKHL_64 (original name was: gnl|Prokka|EPKABKHL_64); gnlProkkaEPKABKHL_65 (original name was: gnl|Prokka|EPKABKHL_65); gnlProkkaEPKABKHL_66 (original name was: gnl|Prokka|EPKABKHL_66); gnlProkkaEPKABKHL_67 (original name was: gnl|Prokka|EPKABKHL_67); gnlProkkaEPKABKHL_68 (original name was: gnl|Prokka|EPKABKHL_68); gnlProkkaEPKABKHL_69 (original name was: gnl|Prokka|EPKABKHL_69); gnlProkkaEPKABKHL_70 (original name was: gnl|Prokka|EPKABKHL_70); gnlProkkaEPKABKHL_71 (original name was: gnl|Prokka|EPKABKHL_71); gnlProkkaEPKABKHL_72 (original name was: gnl|Prokka|EPKABKHL_72); gnlProkkaEPKABKHL_73 (original name was: gnl|Prokka|EPKABKHL_73); gnlProkkaEPKABKHL_74 (original name was: gnl|Prokka|EPKABKHL_74); gnlProkkaEPKABKHL_75 (original name was: gnl|Prokka|EPKABKHL_75); gnlProkkaEPKABKHL_76 (original name was: gnl|Prokka|EPKABKHL_76); gnlProkkaEPKABKHL_77 (original name was: gnl|Prokka|EPKABKHL_77); gnlProkkaEPKABKHL_78 (original name was: gnl|Prokka|EPKABKHL_78); gnlProkkaEPKABKHL_79 (original name was: gnl|Prokka|EPKABKHL_79); gnlProkkaEPKABKHL_80 (original name was: gnl|Prokka|EPKABKHL_80); gnlProkkaEPKABKHL_81 (original name was: gnl|Prokka|EPKABKHL_81); gnlProkkaEPKABKHL_82 (original name was: gnl|Prokka|EPKABKHL_82); gnlProkkaEPKABKHL_83 (original name was: gnl|Prokka|EPKABKHL_83); gnlProkkaEPKABKHL_84 (original name was: gnl|Prokka|EPKABKHL_84); gnlProkkaEPKABKHL_85 (original name was: gnl|Prokka|EPKABKHL_85); gnlProkkaEPKABKHL_86 (original name was: gnl|Prokka|EPKABKHL_86); gnlProkkaEPKABKHL_87 (original name was: gnl|Prokka|EPKABKHL_87); gnlProkkaEPKABKHL_88 (original name was: gnl|Prokka|EPKABKHL_88); gnlProkkaEPKABKHL_89 (original name was: gnl|Prokka|EPKABKHL_89)

Compact view

gnlProkkaEPKABKHL_21 - Region 1 - ranthipeptide

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Location: 2,723 - 24,311 nt. (total: 21,589 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaEPKABKHL_23 - Region 1 - NRPS

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Location: 1 - 39,960 nt. (total: 39,960 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 39960:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg23_11

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaEPKABKHL_29 - Region 1 - lanthipeptide-class-ii

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Location: 19,302 - 41,932 nt. (total: 22,631 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg29_27

allorf_28148_28219

Leader:

MNICNYRIKRILMGMSVFA

Core:

CISD

Core with dehydrations:

CysIDhaD

allorf_40290_40475

Leader:

MSLNFSTYAYITKSPVRYSDAAVFSA

Core:

AAPDYHHPELFPVLLLFHTRELYPHMTEVTCFQTL

Core with dehydrations:

AAPDYHHPELFPVLLLFHDhbRELYPHMDhbEVDhbCysFQDhbL

antiSMASH-DB 4.0 matches for allorf_40290_40475

14.3% NZ_CP058552.1: Lanthipeptides: allorf_1481579_1481740_lanthipeptide

23

YPHMTEVTCFQTL

35

Y H+ + C QT+

6

YKHVQKTNCVQTI

18

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

allorf_28148_28219 - Class II

Cleavage pHMM score: -6.80

RODEO score: 0

Monoisotopic mass: 418.2 Da

Molecular weight: 418.5 Da

Number of bridges: 1

allorf_40290_40475 - Class II

Cleavage pHMM score: -6.80

RODEO score: 0

Monoisotopic mass: 4093.1 Da

Molecular weight: 4095.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4113.7 Da N = 1; 4131.7 Da N = 2; 4149.7 Da N = 3

gnlProkkaEPKABKHL_33 - Region 1 - cyclic-lactone-autoinducer

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Location: 21,874 - 37,295 nt. (total: 15,422 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

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Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.