LOCUS       c00289_gnlPro..         2615 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CLGACMGC_289.
ACCESSION   c00289_gnlPro..
VERSION     c00289_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 03:50:30
            Original ID  :: gnl|Prokka|CLGACMGC_289
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2615
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2615
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[43:2524]"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      44..2524
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
     cand_cluster    1..2615
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /kind="single"
                     /product="Putrescine2spermidine"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2615
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Putrescine2spermidine"
                     /region_number="1"
                     /rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /tool="gutsmash"
     CDS             44..1303
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Sacchrp_dh_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Sacchrp_dh_NADP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Epimerase"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg289_1"
                     /sec_met_domain="Sacchrp_dh_C (E-value: 1.6e-45, bitscore:
                     143.9, seeds: 375, tool: rule-based-clusters)"
                     /sec_met_domain="Sacchrp_dh_NADP (E-value: 5.3e-35,
                     bitscore: 108.3, seeds: 81, tool: rule-based-clusters)"
                     /sec_met_domain="Epimerase (E-value: 2.5e-08, bitscore:
                     21.3, seeds: 96, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGRLLIIGCGGVAGVAIHKCCQNSKTFTEICIASRTKSKCDALKE
                     KLEKTTDTVITTAQVDADNTEELIALIKKYQPDAVLNVALPYQDLTIMDACLACKVDYI
                     DTANYEPEDTDDKEWRAIYEKRCKEEGFTAYFDYSWQWAYCEKFKEAGITALLGSGFDP
                     GVTSVFTAYALKHYFDEIETIDILDCNGGDHGYPFATNFNPEINLREVSANGSYWENGH
                     WVETKPMEIKREYNFPQVGEKDMYLLHHEEIESLAKNVPGVKRIRFFMTFGQSYLTHMK
                     CLENVGMLSTSPIEYEGREIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGKKDGKEK
                     TIYIYNVCDHQECYKEVGSQAISYTTGVPAMIGAALVVDKVWDKDGVFNIEEFDPDPFM
                     DMLNQYGLPWVVDENPQTVA"
     CDS             1391..2524
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Orn_DAP_Arg_deC"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg289_2"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 5.3e-15,
                     bitscore: 43.3, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKWQEIPTPCYVVDEKKLKQNLKILQNLEQETGCHVLLAQKAFSM
                     FSLYPLIGQYISGTTASGIFEARLGKEEMGKENHVFAPAYKEKDMEELVQICDHVIFNS
                     FAQYEKYQHYFLQQECTASAGIRVNPECSTQEGHEIYDPCGPGSRLGVVKSEFPDQLPE
                     GIEGLHFHTLCEQNADDLITTWHAFEEKFGSYLKQVKWLNLGGGHHITRADYQLDELKK
                     LICEIRRKYNVRVYLEPGEAIALNAGYLVTEVMDIVHNGMDILILDASAACHMPDVLEM
                     PYRPPLKDGYLPGEKPHTYRLSSMTCLAGDVIGDYSFAQKKQVGDRLVFEDMAIYSMVK
                     NNTFNGMPLPGIAVLREDGDVEMIKEFGYEDFKGRLS"
     CDS             2496..2615
                     /locus_tag="ctg289_3"
                     /transl_table=11
                     /translation="MKISKADCPKMIKDTTPVQDGFFMPGEFEQHQGCIMIWPE"
ORIGIN
        1 aacttgagat gcgcaaaaag acagcgcagg aaaggagatt aaaatgggaa gattattaat
       61 tatcggatgt ggcggagttg caggagttgc gattcacaaa tgctgccaga acagcaaaac
      121 ttttacggag atctgcattg cgagcagaac aaaatcgaaa tgtgacgcct taaaagagaa
      181 actggaaaaa acaacggaca ctgtcatcac aacagcacag gtggatgcgg acaatacgga
      241 ggaactgatc gccctgatca aaaaatatca gccggatgct gtgttaaatg ttgctttgcc
      301 atatcaggat ttaacaatta tggatgcctg tcttgcatgc aaagtagact atatcgatac
      361 cgcaaattat gagccggagg atacggatga caaagaatgg cgtgccatct atgaaaaacg
      421 ctgtaaagaa gagggattta cagcatattt tgactattcc tggcagtggg catactgtga
      481 aaaattcaaa gaggcaggaa ttacggcatt gcttggaagc gggtttgatc cgggagtgac
      541 cagtgtattt accgcttatg ccctgaaaca ttatttcgat gagatcgaga cgatcgatat
      601 cttagactgt aacggcggcg accacggtta tccgtttgca accaatttta acccggagat
      661 caacctgcgt gaggtttccg caaacggttc ttactgggag aatggtcact gggtagaaac
      721 aaaaccaatg gaaattaaaa gagaatataa tttcccacag gttggcgaga aagacatgta
      781 tctgcttcat catgaggaaa ttgagtccct tgcaaaaaat gtacctggag taaagcggat
      841 ccgtttcttt atgacctttg gacagagcta tctgacacat atgaaatgtt tagaaaatgt
      901 cggaatgctc tcaacatcac cgatcgagta tgagggcagg gagatcgtac caatccagtt
      961 cttaaaagca ctgcttccgg atccggcatc tttaggaccg cgcacggttg gaaaaacaaa
     1021 tatcggctgt atctttacgg gaaaaaaaga cggaaaagaa aagacgattt atatctataa
     1081 tgtctgtgat catcaggaat gttacaaaga agtcggttcc caggcaattt cttatacaac
     1141 aggtgttccg gctatgatcg gtgcagcgtt agttgtcgat aaagtctggg acaaagacgg
     1201 tgtattcaat attgaagagt ttgatccgga tccgtttatg gatatgttaa accagtatgg
     1261 tctgccatgg gttgtggacg aaaatccgca gacggtggca taaaataaga atatgtgtga
     1321 aacgggttgg ataaaatttt aaatttgtca gcttttgcgc agcggatttt tgcagaaatg
     1381 gagaattatc atgaaatggc aggagattcc taccccgtgt tatgtggtag atgaaaagaa
     1441 gctaaaacaa aatttaaaaa tattgcagaa tttggaacag gaaaccggct gccatgtgct
     1501 tctggcacag aaggcatttt ccatgttttc actgtatccg ctgatcgggc agtatatcag
     1561 cggaacgacg gcgagcggaa tctttgaggc gcgccttgga aaagaggaga tgggaaagga
     1621 aaatcatgta tttgcaccgg cttataagga aaaagatatg gaagaactgg tgcagatctg
     1681 cgatcacgtt atttttaatt cctttgcgca gtatgaaaag tatcagcact attttttgca
     1741 gcaggagtgc actgcaagtg ccgggatccg cgtgaacccg gagtgctcca cacaggaagg
     1801 gcatgagatc tatgatccat gcggaccggg atcaaggctt ggtgtggtta aaagtgaatt
     1861 tccggatcaa ctgccggagg gaatcgaagg attacatttt catacactct gcgagcagaa
     1921 tgcggatgat ctgatcacga cctggcatgc gtttgaggag aagtttggca gttatttaaa
     1981 acaggtaaaa tggctgaact taggcggcgg tcatcatatt acaagagcag actatcagtt
     2041 ggatgaacta aagaagttaa tctgtgagat caggcgaaaa tataatgtcc gggtctacct
     2101 ggaaccgggt gaggcgatcg cattaaatgc gggctatctt gtcacggagg ttatggatat
     2161 cgtacacaat ggaatggata tcctgattct ggatgcatcg gcggcatgcc acatgccgga
     2221 tgtgcttgaa atgccatata gaccgccgct aaaagacggt tatctgccgg gcgaaaagcc
     2281 gcatacctac cggctttctt ccatgacctg ccttgccggg gatgtgatcg gtgattacag
     2341 ctttgcacag aaaaaacagg tgggagaccg tctggtcttt gaggatatgg caatttattc
     2401 catggtcaaa aacaatacat ttaacgggat gccgctgccc ggaattgcag tcttacggga
     2461 agatggagat gtcgagatga taaaggagtt tggttatgaa gatttcaaag gcagactgtc
     2521 ctaagatgat aaaagataca acaccggtac aggacggatt tttcatgccg ggtgagtttg
     2581 agcagcatca gggatgtatc atgatctggc cggag
//
