LOCUS       c00293_gnlPro..         4778 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ACELJHIP_293.
ACCESSION   c00293_gnlPro..
VERSION     c00293_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 04:31:05
            Original ID  :: gnl|Prokka|ACELJHIP_293
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4778
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4778
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:4282]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..4282
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..4778
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4778
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(3..914)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg293_1"
                     /sec_met_domain="OAD_beta (E-value: 1e-115, bitscore:
                     375.0, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQESFLTFLGENLQLFLTYTGVYNATPGHLIMILVGLLFIFLAIK
                     YEFEPMLLIPIGFGILIGNIPFKDAGLQVGIYEQGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYTIALQMGFEPNQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPIIQPPFMRLLTTKKERLIRMKPPRAVSSTEKIIFP
                     IVGLLLTCFLVPSGLPLLGMLFFGNLLKESGVTRRLAETARGPLIDTITILLGITVGAS
                     TQATQFLTLNSIKIFGLGALSFV"
     CDS             complement(919..1350)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 64.8; E-value:
                     1.6e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg293_2"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1.5e-21, bitscore:
                     64.8, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSFKYTINGNVYKVHINSVVDDIAELEVNGTPYQVKMEKPAKKQ
                     MVTLKRPAQAPTTASGDPVVSRPAASTTQGAVKTPLPGVILQVKCNVGDTVKRGQTLII
                     LEAMKMENNINADRDGKIIEIKVHKGDSVLEGADLVVIG"
     CDS             complement(1373..2260)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg293_3"
                     /sec_met_domain="OAD_gamma (E-value: 4.8e-13, bitscore:
                     38.4, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINKKIGVLLLFALLISFGAKAQRATSMRINEVLVINEDNFVDDY
                     GKRHGWIELFNTSAGTVNIAGCYLTDDKNNPMKYPIPKGDVLTQIQPRQHTLFWADGQP
                     GRGTFHVNFVLDPSKENYVALYDADGKTLIDEITIPAGQLADISYGRVIDGEKDWAQLK
                     KVTPSTNNLTLDSNEKIDNFRVNDSLGIGMTITAMAVVFLGLFLLYIIFKQIGKLSIAA
                     SKRNVEKAVGSASVTADAGQESGEIFAAIATALYEMSDDNHDIENTVLTIRKVQRAYSP
                     WSSKLYGLRQTPQK"
     CDS             complement(2282..3835)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg293_4"
                     /sec_met_domain="CT_Btheta (E-value: 6.9e-292, bitscore:
                     956.7, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKVKELIALREQARLGGGEKRIESQHKKGKYTARERIAML
                     LDEGSFEEFDMFVQHRCTNFGIEKTKYLGDGIVTGYGTIDGRLVYIFAQDFTVFGGALS
                     EALAMKICKVMDQAMKMGAPVIGLNDSGGARIQEGVNALAGYAEIFQRNILASGVVPQI
                     SGIFGPCAGGAVYSPALTDFNIMTRGTSYMFLTGPKVVKTVTGEDVTQEQLGGASVHTT
                     KSGVAHFAVDTEEDGLQLIRKLLSFLPQNNLEETPLVECKDPIDRLDDVLNDIIPDNAN
                     QSYDMYEVIGTIVDNNEFLEVHADYAKNIIVGFARFNGQSVGIVANQPKVMAGCLDSNA
                     SRKAGRFVRFCDAFNIPLVTLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKITV
                     TLRKSYGGACCVMSSKHLRGDMNYAWPTAEIAVMGPSGAVEVIFAKEVAAAEDPKACAA
                     EKEAEYKKAFANPYNAAQYGYIDDVIEPRNTRFRIIRALQQLQTKKLSNPAKKHDNLPL
                     "
     CDS             complement(3872..4282)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg293_5"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.3e-27, bitscore:
                     85.0, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELTHIEHLGIAVKSIEACLPYYEGVLGLKCYNIEVVADQKVKTA
                     FFKVGQTKIELLEPTSEDSTIAKFIEKKGEGIHHIAFAVPDVQAALNEAEEKGVKLIDK
                     APRGGAEGLNIAFLHPKSTCSVLTELCMPGNK"
     CDS             complement(4411..4776)
                     /locus_tag="ctg293_6"
                     /transl_table=11
                     /translation="FFQDYFPGYKRVKLNLKGVLSGGLPQTIPGKGYEAGYFRTPAYKR
                     VDIGLSYQLAGGTDAIMDRGFFRHLKNIWLGLDVFNILDIKNVSSYYWITDVYNVQYAV
                     PNYLTGRQLNVRLIVDF"
ORIGIN
        1 atcacgaatg ataacgcacc tagaccgaag atcttgatag agttcaatgt caagaactgt
       61 gtagcttggg tagaagcacc taccgtgata cccaataaga tcgtgattgt atcgatcagc
      121 ggaccacgag cagtctctgc caaacgacga gtcacgccac tttctttcaa taagtttccg
      181 aagaatagca tacctaacaa tggtaaaccg gacggaacca agaagcaagt aagcaacaaa
      241 ccaacgatcg ggaagataat tttttccgtg gaagaaacgg ctctcggcgg tttcatacgg
      301 atcaaacgct ctttcttagt cgtaagcaat ctcatgaacg gcggttggat aataggaacc
      361 aacgccatgt atgagtaagc ggataccgcg atcgcaccca ttaagtttgg cgctaactta
      421 gaagaaagga agatagccgt cggaccgtca gcaccaccga taataccgat agcacctgct
      481 tggttcggct caaatcccat ttgaagagca atcgtataag caccaaagat accgaactga
      541 gccgctgcac cgatcaacat caacttagga ttggagatca atgcggagaa gtcggtcatg
      601 gccccgatac cgaggaagat caatggcgga taccatccag aggtcacacc ctgatagagg
      661 atgttcagta cagacccttg ctcataaata cctacctgaa gacctgcgtc cttgaaaggg
      721 atattaccga tcaagatacc aaatccgata gggatcaaaa gcatcggttc aaactcatat
      781 ttaatcgcca ggaatataaa caacagacct accagaatca tgatgagatg tcccggagta
      841 gcgttgtata cacctgtata ggtaaggaac agttgtaagt tctcacctaa gaatgttaaa
      901 aagctctctt gcatagtctt atccgattac tacgagatca gcaccttcta gcacagagtc
      961 tcccttgtgt actttaattt ctataatctt accgtcacgg tctgcgttga tgttgttctc
     1021 catcttcata gcctcaagga taatcaaggt ttgacctctc ttcacggtat cacctacgtt
     1081 gcacttaacc tgcaagataa cacctggtag cggagtctta acagcccctt gtgtcgtaga
     1141 agccgccgga cgggaaacaa cagggtctcc acttgccgta gtcggggctt gagctgggcg
     1201 tttcaatgta accatttgtt tctttgccgg tttctccatc ttaacttggt agggagttcc
     1261 gtttacttca agttctgcga tatcatccac tacagaattg atgtgtactt tatatacgtt
     1321 accgttaatg gtatatttaa aacttttcat tctttagttc gtttttagat aattactttt
     1381 gaggagtttg acgtaagcca tagagttttg aactccaagg agaataagct cgttgtacct
     1441 tacgtatagt gagaaccgtg ttctcgatat cgtgattatc atcgctcatt tcgtatagag
     1501 cggtagcgat ggcggcgaat atctctccag attcttgtcc tgcgtctgcg gtaactgaag
     1561 cagaacctac ggccttctct acgttacgtt tgctagcggc gatagataat ttaccaatct
     1621 gcttaaagat gatgtacaac aagaacagac ctaagaatac aacagccata gccgtgatag
     1681 tcataccgat acctaaagag tcattaaccc tgaagttatc gattttctca ttggagtcca
     1741 acgtcaagtt gttcgtactg ggagtgactt tctttaactg tgcccagtct ttttcgccat
     1801 caatcactct accgtagcta atgtctgcta attggcctgc cggaattgtg atctcatcga
     1861 tcaaggtctt accgtcggcg tcatataagg caacataatt ctccttagag ggatctaata
     1921 cgaagttaac gtggaatgta cctctgcccg gctgtccgtc agcccaaaat agtgtgtgct
     1981 ggcggggttg gatctgagtt aatacgtctc ccttcgggat cggatatttc atgggattgt
     2041 ttttgtcgtc tgtcaaatag catcccgcga tatttaccgt tccggccgaa gtgttgaaca
     2101 actcaatcca gccatgacgt ttgccatagt catcaacgaa gttatcctcg ttgataacca
     2161 atacttcatt aattcgcatg gaggttgcgc gctgtgcctt tgccccgaat gaaatgagca
     2221 aagcgaacaa tagcaatacc cctattttct tatttatcat attcatctcc tgttttacgg
     2281 attatagagg caggttatca tgtttcttag caggattgct tagcttctta gtctgcaatt
     2341 gctgcaaagc acgaattata cggaaacgag tgttacgcgg ttcgataaca tcgtcgatat
     2401 aaccgtattg agctgcgtta taaggattag cgaatgcttt cttatattca gcttctttct
     2461 ctgctgcgca tgctttcgga tcttcagcgg ctgctacttc cttggcgaag ataacctcta
     2521 cggcaccgct cgggcccata acggcgatct cggctgtcgg ccatgcgtaa ttcatatcac
     2581 cacgtaaatg tttagaactc atcacgcaac aagctccacc gtaagactta cgcaatgtaa
     2641 cggtaatctt aggaacggta gcctcaccat aagcgtaaag caatttagcg ccatgaagga
     2701 ttacaccgtt gtattcctga cctgtacccg gaaggaatcc cggaacgtca accaatgtta
     2761 ccaaaggaat gttgaacgcg tcgcagaaac gaacgaagcg acctgcttta cgcgatgcgt
     2821 tgctatccaa gcatcctgcc ataaccttcg gctggttggc tacgataccg acagattgtc
     2881 cgttgaaacg agcgaaacct acgatgatat tcttagcgta gtccgcatgg acctctagga
     2941 actcgttgtt atcgacgatc gtaccgataa cctcatacat atcgtaagat tggttagcgt
     3001 tatcgggaat gatatcgttc aatacatcat ccagacgatc gatcgggtct ttacactcaa
     3061 ctaacggcgt ttcctctaag ttgttctgag gtaagaaact gagcaattta cgaatcaatt
     3121 gcaaaccatc ctcttctgta tcaacagcga aatgggctac acctgatttt gtagtgtgta
     3181 cgctggctcc acccaactgc tcttgggtta catcctcacc tgttacggtt tttactactt
     3241 tcggacctgt caagaacatg tagctggtac cacgtgtcat gatgttgaag tctgtcaaag
     3301 ccggagaata aacggctcca ccggcgcaag ggccgaagat accggaaatc tggggaacaa
     3361 caccagatgc caagatgtta cgttggaaaa tctccgcata tcctgccaag gcgtttacac
     3421 cttcttggat acgagctccg ccggagtcgt tcaaaccgat aacgggagcg cccatcttca
     3481 tggcttgatc cattacttta cagatcttca tagccaaagc ctcagacaag gctccaccga
     3541 atacggtgaa atcttgagcg aagatatata ccaaacgtcc gtcgatcgta ccataacctg
     3601 ttacgatacc atcgcctaaa tatttagttt tctcgatacc gaagtttgta caacggtgtt
     3661 gaacaaacat gtcgaattcc tcgaaactac cttcgtcaag caacatggcg atacgctcac
     3721 gagcggtata tttgcctttt ttgtgctgtg attcgattct tttctctcct ccacccaagc
     3781 gagcttgctc acgaagtgcg atgagctctt ttactttttc aagctggtta ctcattgtat
     3841 tttgatttgt tgttagaaat gaacggatta attatttgtt gcccggcatg caaagttctg
     3901 tcaatacgct gcaagttgat ttcgggtgaa ggaaagcgat gttcaagcct tccgcgccac
     3961 cacgaggagc tttatcgatt aatttaacgc ctttttcctc agcctcgttc aaagctgcct
     4021 gtacatcagg taccgcgaat gcgatatggt ggataccttc accttttttc tcgataaatt
     4081 tcgcgatcgt gctatcttcg ctggtcggct ctagtagttc aatcttagtc tgtcctacct
     4141 tgaaaaatgc ggtttttact ttttggtctg ctactacttc gatattgtag cattttaatc
     4201 caagaacgcc ttcataatag ggtaaacaag cctcgatact cttcactgcg atacctaaat
     4261 gttcgatatg tgtaagttcc atgtcaataa taatttaaag atttagtaaa tgtttgtttc
     4321 tattgtgtgc aaatgtacac tactttttat gaatgaggaa ataatttttt gtttttatct
     4381 cccagtaaat gtgtttaaag atacatttta ttaaaaatcg acaatcagac gaacgttcaa
     4441 ttgacgtccg gtcagatagt ttggaactgc gtattggaca ttatatacat ctgttatcca
     4501 atagtaggaa ctaacgtttt tgatgtctaa tatgttaaac acatccagac ctagccaaat
     4561 gtttttcaaa tggcggaaga agcccctgtc catgatagcg tccgtgcctc ctgccaattg
     4621 ataggagagg ccgatatcta cacgtttgta ggcgggcgta cggaaatagc cggcttcgta
     4681 tcctttgccc gggattgtct ggggaagtcc tccggataga actcctttca gatttagctt
     4741 gactcgttta tagcccggga aataatcttg gaagaaga
//
