LOCUS       c00233_gnlPro..         5563 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LMJJPEIB_233.
ACCESSION   c00233_gnlPro..
VERSION     c00233_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 06:08:20
            Original ID  :: gnl|Prokka|LMJJPEIB_233
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5563
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5563
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[32:3008]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      33..3008
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5563
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5563
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             33..2273
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg233_1"
                     /sec_met_domain="Gly_radical (E-value: 2.7e-46, bitscore:
                     145.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.5e-282, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREMQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             2274..3008
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg233_2"
                     /sec_met_domain="Radical_SAM (E-value: 6.4e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             3207..3785
                     /locus_tag="ctg233_3"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPDADLDSLASIIESSKAAKGELLLDNG
                     QISKYLYYVESGLLRQFYYKNKRDITEHFSCEGNIVICIASTFQREPTRLLIEALEPTV
                     YHRISFSEFSDLLKTSLAINRLYQKILEWALMLSQEKADSWRFESARKRYLRFQQEYPE
                     AAKRASVNHIASYLLMTPESLSRVRAGVL"
     CDS             3893..5173
                     /locus_tag="ctg233_4"
                     /transl_table=11
                     /translation="MKIEKITGREILDSRGNPTVEVDILLESGVMGRASVPSGASTGEN
                     EALELRDGDKKRYGGKGVLKAVENINTIIAPALKGMSALDQIGIDHAMLALDGTKTKSK
                     LGANAILGVSLAVAKAAANYLDIPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIAFQE
                     FMIRPVGAASFKEGLRMGAEVFHALKKVLHDRGLSTAVGDEGGFAPNLEGTEDALNSII
                     AAIKAAGYEPGKDVMIGMDCASSEFYKDGIYDYTKFEGEKGVKRTSDEQVDYLEKLINE
                     YPIDSIEDGMSENDWAGWKKLTDRIGNHCQLVGDDLFVTNVEFLSRGIKEGCGNSILIK
                     VNQIGSLTETLNAIEMAHRHGYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSD
                     RMAKYNQLLRIEEELGDRAVYGYKRIN"
     CDS             5394..5561
                     /locus_tag="ctg233_5"
                     /transl_table=11
                     /translation="MEPIINSQLPEFKVQAFQNGNFKTVTNEDVLGKWAIFFFYPADFK
                     FFFPKELVDMG"
ORIGIN
        1 cggtaaaaat gtggtattag ataagattgt ttatgaaatt tgagaatgag tgctgggatc
       61 actttaaggg tgagacctgg aaaagagaga tcaatgtaag agattttatc cagagtaact
      121 atacgcctta tgaaggggat gattcctttt tagtcgcttc ttccgagaag actcgtaagg
      181 tctggaacaa gctgaccgag atgtttaagg tcgagaggga aaagggagtt tatgacgccg
      241 agacaaaatt acctcaaggg atcgatgtat atggcccggg ctatattgat aaggagaatg
      301 aggtgatcgt tgggttacaa accgatgctc cgctgaaacg tggtatcttc ccgaaaggcg
      361 gtatccgtat ggtcgagaat agcttggaag cttatggtta tcatttggac ccgatgacga
      421 aagagatctt tacgaagtat cgtaagactc ataacgaggg cgttttctcc gcatatacgg
      481 aagagatgat tgccgcacgt cgttcggcta ttatcaccgg tctgcccgac gcgtatggta
      541 gaggacgtat tattggtgac tatcgtcgtg tcgctttata tggtacggca atcttgatcg
      601 aggagaaaaa gcgtttcttg aatcgtttgg atatccaaga gatcacggaa gagatcatcc
      661 agagccgtga ggagatttcc gaacagatca aggctttgaa agcgttcgag aaaatgtgcg
      721 ctagctatgg ctttgatgtg actcgtccgg cacaaaacgc tcgtgaggca gttcagtttg
      781 tgtacttagc ttatctggcg gcggtaaaag accaagatgg cgcggcgatg tctatcggac
      841 gtacttctac gtttttggat atttatatcg agaaagacat ccgtgagggt aagttgaccg
      901 aagaggaggc gcaagaattg gtagaccagc tgatcatcaa gttgcgtatc gttcgtttct
      961 tacgtacgcc ggaatacaat gatttgttct ccggtgatcc ggtttgggta acagagtcct
     1021 tgggcggcca aggagtggat ggccgttctt tggtaacaag aacttcctat cgttatctcc
     1081 atacattata taatctaggt ccggcaccag aacccaattt gacggttctg tggtttaaga
     1141 acgctcccga gaactggaaa cgtttctgtg ccaaggtctc tatcgatacg tcggctatcc
     1201 aatatgagaa cgacgatttg atgcgtccgg attatggaga tgattatggt atcgcttgtt
     1261 gcgtttctcc tatgaagatc ggtaagcaga tgcagttctt cggagcccgt gcgaatttgg
     1321 cgaaatgttt attatatgcg atcaacggag gacgggatga gcgcagcggc gtacaggtag
     1381 ccccgatgtt cgagccggtt cgcggcgagt atctggaata cgatgaggtg atggctaagt
     1441 atgagcaaat gatgcgctgg ttggcgaaag tgtatgtgaa cgccttgaag ataatccatt
     1501 atatgcatga taaatacgcg tatgaggcat tcgagatgtc gcttcacgat ggggatgtag
     1561 agcgtatccg tgcgacaggt atcgccggtc tttctatcgt tgccgattcg ttggctgcta
     1621 tccgtgatac gaaagtacgt gtgatccgtg acgagcgtgg cttggcggtt gatttcgaac
     1681 gtgagggcga atatgttcct ttcggtaata acgatgatcg tacggatagt atcgctgtag
     1741 agatcaccga gaagttcatg gaatatttgc gtcagcacga gacctatcgc catgcgaaac
     1801 ctacgcagtc catccttacg atcacctcta atgtggtata tggtaagaag actggtacga
     1861 caccggatgg ccgtatcggt ggaactcctt tcgctccggg cgcgaaccct atgaacggtc
     1921 gtgatacgaa aggtgctatc gctgcgctag cttcggtagc gaagcttcca ttccagcatg
     1981 cgcacgacgg catctcttat actttcgctg tttctccggc tacgttggga aaggagcgtg
     2041 aaatgcagat caataatctg gttagcttat tggatggcta ttttactccg gatggaggtc
     2101 agcatctgaa tgttaacgta tttgataagg atttgttgat cgacgctatg gaacatcccg
     2161 agaaatatcc gcaacttacg atccgtgtat ccggatatgc ggtaaacttc gtgaaactga
     2221 ctcgtgaaca acagttggat gttatttcaa gaaccattaa ccattcactg taagtgataa
     2281 aaggtaagat acattcatta gagagtttcg ggaccgtaga cggtcccgga attcgctttg
     2341 tcgtttttat gcagggctgc ccgttgcgtt gcctgtattg ccataacccg gatacttgga
     2401 acccgaaagg gaaggtgaaa tatcagatga ctcccggcga gctattgacg gaagttttac
     2461 ggtataagag ttttatcgcc cgtggggggg tgactgttac cggtggggaa cctttattgc
     2521 aacctgagtt tctgaaagag tttttccgtt tgtgccaaga gcaaggtctt cacacagctt
     2581 tggacacatc tggtttcgta tgtacctcaa aagcttggga agtcttggat tatgcggact
     2641 tagtgttatt ggacatcaaa accttaaacc cggatttaca tccgttactg gcgggtgtca
     2701 agcaggataa tacgcttctg ttcttggatg aattggagag acgtggcata gatacttgga
     2761 ttcgccatgt tattgtcccc ggatatacgg ataatgacga gtggctggag gcgttagctc
     2821 ggtatgtcag ttcatataaa gtggttcgca aagtggaatt attgccttat catacgatgg
     2881 gaacctataa atatgagcaa ttaggcctcg attatccgtt aaagggggtg gaacccttat
     2941 caaaagaacg tttagataat gcgaaagcga tattctctcg ctacaaacca gaaaataaca
     3001 aggcctgatc tctcgatcaa gccttgcgat tatgcaacag ctaatcaggg aaaacaatct
     3061 aatccgatta gcttttaaac gatagtaata attttttgcc tagactaacc ttgactattt
     3121 aatatgtgtt tgttttgtta tatgcaacaa atatctattt ttgtaaggag ataaaaaatg
     3181 atatagatca gaaaatgatg aatagtatga agacaccaca agaaatagcc gaaatgatat
     3241 ccaaacgttt ttatccatta cccgatgcgg atttggattc tctggcctcg atcatagaat
     3301 caagtaaggc tgcgaaagga gaattattgt tggataacgg acaaatatct aaatatttat
     3361 attatgtgga atcgggatta ttacgccagt tttactataa gaataaacgt gatattaccg
     3421 agcatttctc ctgtgaggga aatatcgtta tttgtatagc cagcacattc caaagagaac
     3481 cgacgcgttt attaatcgag gcattggaac cgacggttta tcaccggatc tctttttcgg
     3541 aatttagcga tttattgaaa acatctttag ctattaatag attgtaccag aaaatactgg
     3601 agtgggcttt gatgctttcg caagagaaag cggactcttg gcgttttgag tcggcaagaa
     3661 aacgttatct gcgttttcaa caggaatatc cggaagcagc gaaacgtgct tctgtcaatc
     3721 atatcgcctc gtatttattg atgacacccg aatctttgag ccgggtgcgg gcgggagtgt
     3781 tgtagtctct acctacattc aggtattatt gaggtgaaaa tagcaatttg tggtgatttc
     3841 acggttcaag taatccatct atatttgcgt cagtttaaaa aacgtagaag caatgaaaat
     3901 agaaaagatt acaggacgtg agatcctaga ttcaagaggt aacccgacag tagaggttga
     3961 tattttacta gagtccggcg ttatgggccg tgcgtctgtg ccttccggtg cgtccaccgg
     4021 cgagaacgag gctttggagt tgcgtgatgg cgacaagaag cgttatggtg gtaaaggcgt
     4081 attgaaagcg gttgagaata tcaatacgat tatcgctccg gcattgaagg ggatgtctgc
     4141 cttggatcag ataggtatcg atcatgcgat gttggctttg gacggtacga aaactaaatc
     4201 gaaactaggt gcgaacgcta tccttggcgt ttctttagct gtagcgaaag ctgccgcaaa
     4261 ttatttggat attcctttat atagatatat tggtggaacg aatacctacg tgatgcctgt
     4321 tccgatgatg aatataatca atggcggttc gcatagcgac gctccgatcg ctttccaaga
     4381 gtttatgatt cgtccggtag gtgccgcttc tttcaaggaa ggcttacgta tgggcgctga
     4441 ggtattccat gcgttgaaaa aggtattgca cgatcgtggg ttaagtaccg ctgttggtga
     4501 tgagggtggt ttcgccccga atttggaagg tacggaagat gccttgaact caattatcgc
     4561 cgctatcaag gcggctggtt atgaaccggg caaggatgtg atgatcggta tggattgcgc
     4621 ttcttctgag ttttataagg atggaatcta cgattacacg aagtttgaag gagaaaaagg
     4681 agtgaaacgt acttccgacg aacaagtgga ttatctggaa aagctgatca acgaataccc
     4741 gatcgattcc attgaggatg gtatgagtga gaatgattgg gctggttgga agaaactgac
     4801 agatcgtatt ggtaaccatt gccaattagt cggagatgat ttgttcgtga caaatgtaga
     4861 gttcctttca aggggtatta aagaaggttg cggtaactca atcttgatca aggtaaatca
     4921 gatcggttcc ttgacggaga cactgaacgc tatcgagatg gctcaccgtc atggatacac
     4981 gaccgttact tcacaccgtt ccggtgagac ggaagacgct acgatcgccg atatcgctgt
     5041 cgctaccaat agcggacaga tcaagacggg atcattaagt cgttcggacc gtatggctaa
     5101 atataaccag ctgcttcgta tcgaggagga attgggagac cgtgccgtat acggatacaa
     5161 aagaatcaat taagtatata aagtaaatta taatcgctaa ttatgtagta atccaaaacc
     5221 caagatattg aagggcttat ccgtgaggat aggccctttt tattgattga taaagtatta
     5281 tctataacct cataaaatat atcgattaga aaaattgttt taatgaagaa atatgttata
     5341 tttttgtatc ggaaataaaa acaaatagta ttcatttaaa aataggaaca agtatggaac
     5401 cgattattaa ttctcaactt ccggagttta aagtacaagc ttttcaaaat ggtaatttca
     5461 agactgtaac caacgaggac gttttgggta agtgggctat ctttttcttc tacccggccg
     5521 attttaaatt tttttttcca aaagagttgg tcgatatggg tga
//
