LOCUS       c00228_gnlPro..         3924 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DIOJKBDK_228.
ACCESSION   c00228_gnlPro..
VERSION     c00228_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 05:12:21
            Original ID  :: gnl|Prokka|DIOJKBDK_228
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3924
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3924
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[735:3727]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      736..3727
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3924
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3924
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..418
                     /locus_tag="ctg228_1"
                     /transl_table=11
                     /translation="LPPIRHICGLGASVGCERVERTFNTPRLKSQICLIRGNGFVCSSL
                     NELNSNPLTFCSILFIVRILIRLTEVSGMNHMKYKTLIADRLGNSCLDAGFFISDKIIF
                     YPISLIRSNFKSLLSHVLKKMFFQTCSKERKIKL"
     CDS             736..2988
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg228_2"
                     /sec_met_domain="Gly_radical (E-value: 1.8e-42, bitscore:
                     132.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.7e-268, bitscore:
                     877.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERIGNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
     CDS             2975..3727
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.6;
                     E-value: 3.2e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg228_3"
                     /sec_met_domain="Radical_SAM (E-value: 2.4e-32, bitscore:
                     100.3, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNAMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     DTWKMTEENGAVWKSADALLNQALRYRPYWKNGGGITVSGGEPLLQIDFMLEFFKKAKE
                     KGIHTTIDTAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDNKYHKVLTGRSNENILD
                     MIRYLSDIKKPIWIRHVLVPERSDKDEYLTRLADFIHSLDNVEKVEILPYHTMGIYKWK
                     ELGLEYPLEGIEPPTKERVENAKKILAI"
ORIGIN
        1 attgccccca ataaggcata tttgtggtct cggagcaagt gttggatgcg agcgagtaga
       61 gcgaacattc aacacgccgc gactaaaatc acaaatatgc cttattcggg gcaatggctt
      121 cgtctgtagt tcgcttaatg aattaaatag taaccccttg acattttgtt ctattttgtt
      181 tatagttagg atacttatac gactgacgga agtaagtgga atgaaccaca tgaagtataa
      241 gactcttata gccgaccgtc tgggcaattc ttgtctggat gctggctttt ttatttctga
      301 taagataata ttctatccaa tatccctgat aagaagtaac tttaaatcgt tattaagtca
      361 cgtgctaaag aagatgtttt tccaaacatg ctctaaagag cgaaaaataa aattatagtt
      421 gtatgcttgt cattattgta cttatatcag tacatttgta cgatttgata atattttatc
      481 aacgctctaa aaccctttat tttaaaggct ctacaagcat ttttgtgtat atgttcaaaa
      541 aaaagaacac ataatatcaa aaaattgttt gacgattgcc aaaaatcatg ttaataatgt
      601 tgttagatga ttaaaactta aataagttaa aactaatcat agtaaatcat ctgaacaatt
      661 ttgttgattt tttagcaaac atatttcaaa accttgactt aaggattttt ttaacaaaca
      721 gggaggtcaa ttaaaatgtt acaaaaagaa caatggcagg gatttaatgg acgaatctgg
      781 agagaagaaa ttaatttaag agagttcatc caggataact acactccata tgatggagat
      841 gcttctttcc tcgaaggccc taccgaggct actgacacac tgtggggcga actgcagaag
      901 ctgcagaaag cagagcgtga aaagggtggt gttcttgata tggatactga tatcgtatca
      961 agccttactt cccataaacc gggttacatt tctgagtctt taaaagagaa agaacagatc
     1021 gtaggtcttc agacagataa acctttaaaa cgtgcattta tgccatttgg tggtatccgt
     1081 actgctgaag aagcttgtac aacttatggt tacacaccaa atccagaatt ccacaagatt
     1141 tttacagatt atcataagac acataaccag gcagtatttg attcttatac tcctgaaatg
     1201 aagaaagctc gtcacaataa gatcattact ggtcttccag atacttacgg acgtggacgt
     1261 atcgttggtg actaccgtcg tgtagctctt tacggtattg acctgttagt tgctgacaag
     1321 caaaaagatt tcgcaaactg cggtgacgga acaatgacag atgacgttat ccgtttaaga
     1381 gaagaaatct ccatgcagat taaagcatta aaagatatga aagttatggc tgaaagctat
     1441 ggctatgata tttccgaacc tgcttctaac gcaaaggaag ctgttcaatg gacgtatttc
     1501 ggataccttg cagcaatcaa gacacagaac ggtgctgcta tgagtatcgg acgtatcgct
     1561 actttccttg acatttatat caaacgtgat atggataagg gaatccttac agagaaagaa
     1621 gcgcaggaat taatcgacca tatgactatg aagttccgta tggttaagtt cggacgtatc
     1681 ccagcttaca accagctctt ctccggtgac cctgtatggg caacacttga agttggcgga
     1741 cttggacagg acggtcgttc catggttaca aaaactgact accgtttcct ccacacatta
     1801 gaaaatatgg gaccatcccc agagccaaac ctcacagtac tttactctga gcgtcttcca
     1861 aaagcattta gagattacgc agctcatatt tctattacaa caagttcaat ccagtatgaa
     1921 aatgatgatg taatgcgtcc agtatggggc gatgattact ccatctgctg ctgtgtttct
     1981 gctacacaga ctggtaagga aatgcagttc ttcggagccc gtgcaaacct tgcaaaatgt
     2041 ctcctctacg caatcaatgg tggtgtagat gaaaagactg gtcagcaagt tggtccggaa
     2101 tacaaaccga ttacttccga atatcttgac tatgatgaag taatgcacaa atatgatatc
     2161 atgatggact ggttagcagg tttatatgta aatacattaa atctcatcca gtacatgcat
     2221 gacaaatatt actatgaata tgctttgatg gccttaatcg atacaaatgt acgtcgtaca
     2281 ttcgctacag gtatcgcagg attctcacat gtagtagatt ccttaagtgc gattaaatat
     2341 gccaaagtaa aaacagtccg caatgaagaa ggacttgttg ttgattatga gacaactggc
     2401 gacttcccta aatacggaaa tgatgatgac cgcgctgatg atatcgctgt atggttactt
     2461 cagacattta tgaagaagct tgaaaaattc catacttacc gtgattctga acctacaact
     2521 tccatcctta ctatcacatc aaacgtagta tatggtaagg caacaggttc ccttccagac
     2581 ggaagaaagg ccggagaacc tctttcccca ggagcaaacc caagttatgg tgctgagcag
     2641 aacggacttt tagcttcctt aaactctgta gcaaaacttc cttacgaatg ggcattagat
     2701 ggtatttcca atacacagac aatcaaccct gatgcactcg gtcattccga agaagaacgt
     2761 attggtaact tagtaaatgt tatggacggc tacttcgcac agggtgctca ccacctaaac
     2821 gtaaatgtat ttggtgttga gaaactgatt gatgctatgg aacatccgga aaaagaagaa
     2881 tacgcaaact ttacgatccg tgtttcaggt tatgcagtta aattcatctc tctgacaaaa
     2941 gaacagcagt tagatgtaat tgccagaaca tgccatgaca gaatgtaata aaaatgcgat
     3001 ggctcttgtt cattctacgg agagttttgg ttcggtggat gggccgggag ttcgttttat
     3061 tgtttttttg cagggttgcc ctttgcggtg ccagttctgt cacaatccag atacatggaa
     3121 gatgacagaa gaaaatggtg cggtatggaa aagcgcagat gcgctgttaa atcaggcact
     3181 ccgctatcgt ccttactgga aaaatggcgg aggtattact gtcagtggtg gagagccact
     3241 tttacagatt gattttatgc ttgagttttt taaaaaggca aaagaaaaag gaatccacac
     3301 tactattgat actgccggag gtccttttac gagagaagaa cctttctttt ctaaatttca
     3361 ggaattgatg aaatatactg acctgttatt agtggatatc aaacacattg ataacaaata
     3421 ccacaaagtt ttaactggtc gtagcaatga gaatattctt gatatgattc gctatctttc
     3481 cgatatcaaa aaaccaattt ggattcgtca tgtccttgtt ccggaacgaa gcgataaaga
     3541 tgaatatctg actagattag ctgatttcat tcactctctt gataatgtgg aaaaggtgga
     3601 aatcctgcct tatcatacga tgggaattta taaatggaaa gaacttggat tagagtatcc
     3661 actggaggga attgagccac caacaaaaga acgagtggag aacgccaaga aaattcttgc
     3721 tatttgattt gccgacaaac agaatcctaa aaacccccat atttgtgact ttgaagatag
     3781 attgcgttga atacttattc atcttcaatc tatccttgaa gtcacaatat ggggggtctc
     3841 tggtatgctc ccttttaggt agacagttga aaaaaataaa actgtttatc tgaaaggggg
     3901 cattttttat ggataagatt tctc
//
