LOCUS       c00063_gnlPro..         3664 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MFCOMFFP_63.
ACCESSION   c00063_gnlPro..
VERSION     c00063_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 00:07:37
            Original ID  :: gnl|Prokka|MFCOMFFP_63
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3664
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3664
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[58:3014]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      59..3014
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3664
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3664
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(59..784)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.8;
                     E-value: 2.8e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg63_1"
                     /sec_met_domain="Radical_SAM (E-value: 5.6e-32, bitscore:
                     98.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANDIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDTRLLGESLGKYQQIQRVEILPYHRLGVHKYEAMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(786..3014)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg63_2"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1170.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKIDETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 gtgttagcaa agttaatact ttattccttg ctgacacagt ttaatttaca tcctcgtttt
       61 agttggttat cacattcgtg aaatactctt tgaacagttt ttctgcccgt tccagttgtt
      121 ccggagtatt ttccttcact tctttcaatt tgtaatccca tcccatggct tcgtatttgt
      181 gaacacccag acggtgataa ggaagtattt ccacccgttg tatctgctgg tatttgccaa
      241 gactttcacc cagcaggcgg gtgtcttctt ccgaatcgct gtaaccgggg accagtacat
      301 agcgcagcca gaaggggtgt tcatgttctt cgagccaggc agcggtgcgg agtgtctgtt
      361 cgttgcttcg tcccgtcagc gtacggtggc gttcgggatt gaattgcttg atgtccagca
      421 gcacgaggtc agtcagacta aagagctctt ccaccttttc attccagatg cctccgttac
      481 tgtccagaca gatatggatg tcgttcgcct tcagctcctt gaaaagagga atcagcgctt
      541 ctgcttgcag ggtaggctca ccgccggaga aagtaatacc tcctttcttc ccgaagaatg
      601 ccttttggct gatagccatt tgcagaatct cttccggagt ggtgggagtg cctcctttta
      661 catcaatcgt atccggattg gcacaataca gacaacggaa cggacatcct tgcaggaaaa
      721 cgacgagccg gagacccggc ccgtcgaatg ttcccatact ttcgtaggag tgtacattta
      781 tcatattaca ttctttcgtg gaatgaacgg ctgatgactt ccaactgatg ctcgcggctc
      841 aacttcacga agttcacggc atagccggaa acgcggatgg tgagttgcgg atacttttca
      901 ggatgttcca tggcatcttc cagcatttcg cggttcagta cgtttacgtt caaatggtgg
      961 gcacctttgg tgaagtagcc gtccatcatg gttaccaggt tctctacacg ttcttccggt
     1021 gtcgggccca gtgatttggg aacaatggag aacgtattgc tgatgccgtc ctgcgagtcg
     1081 cggtagcgca atttggctac ggagctcaag gaagcgatgg caccgttctt gtcacgtccg
     1141 tgcattgggt tggcacccgg agcaaaggca acacctttgg cgcgtccgtc cggagtggca
     1201 ccggtcttct tgccgtacat cacgttggag gtgatagtca gcaaggaaag ggtgggacgg
     1261 gcattcttgt agacaggcag ctttttcagt tcttcgctga aatagtaaac gaggtctact
     1321 cccaggtggt ccacacggtc gtcattattg ccgaagcagg ggaatgagcc ttcggtgtcg
     1381 aaaccttcgg tcaggccgat ttcgttgcgg cgtgcagtga ctttggcata tttgatggcg
     1441 gacagtgagt ctatggcgat ggaaaggccg gcaacaccgt atgccagatt gatgcgtggg
     1501 tcggtatcga tgaatgccat ctgggctttc tcataataat acttatcatg catgtagtgg
     1561 atgatattca tggcctcgtt gtagacacgg gctatttcag tcagtacctt cttgtagttg
     1621 gccataactt cctcaaactt cagtgtctcg ccggtcaata cgggaatacc tttcaccatg
     1681 acagttccgg tgttttcgca acggccaccg ttgatggcaa gcagcagggc cttggcaagg
     1741 ttggcacgtg cgccgaagaa ctggatttgc ttgccgatgg cttggtagga tacacagcag
     1801 gcaatgccgt aatcgtcgca gttgcgcact tcgcgcatca ggttgtcgtt ttcgtactgg
     1861 atggagctgg tatctacaga aactttggcg cagaaatcct tgaagccttc cggcagttcg
     1921 ggactccaca atacggtcaa gttcggttcc ggagaaggac cgaggttgta tagcgtctgc
     1981 aaaaagcgga aagaagtctt ggtgaccttc acgcgtccgt cgttgaagcg tccgccgatg
     2041 gcttcggtca cccaagtcgg gtcgccggca aagatatcat tgtacgactg catgcgcaga
     2101 tggcgtacca tacgtagttt gatgacgaac tggtctatca gttcctgtgc aaacgtctcg
     2161 tcaatcttgc cgtgagccaa atcgtattcg atgaaaatgt cgaggaacga agatacgtta
     2221 cccagtgaca tggctgctcc gtcctgctct ttgatggcag caagatatgc catgtatacc
     2281 cattgcacgg cctcttgagc tgatgtagcc ggatggctga ggtcgagtcc gtaatattcg
     2341 cccataacct tgatgtcctt caaagctttg atttgttctg ccacttcttc gcgtagacgg
     2401 atgcgtgctt cggtcatcgg gccggtgagg ttgcgcaagt cctcctgctt ggcttcaatc
     2461 agacggtcga tgccgtagag agccaatcgg cggtagtcgc cgatgatacg tccgcgggca
     2521 tagttgtcgg gcaatccggt aaggaatccc agtgagcgga aggaacggat ttcttcggta
     2581 tatacatcga atacgccgtc gttatgtgtt ttgcggtaat gggtgaagat gtcttttact
     2641 ttatcgtcca cttccactcc gttttcacgg catgcttttg ctaccacgtt gatgcctccg
     2701 aaaggtttga tggatcgttt caacagttca tcagtctgca aaccgacaat aagttcattg
     2761 tccttatcaa tatacccggc tttatgagaa gtgatggtag atacggtggt attgtccagt
     2821 gaacggacgc cattgttgtt cctttcttct tcgagagcct tcagacagat gtcccagata
     2881 cgtttggtac gttcggtagg tccctgcaag aaagatgcat ccccggtata gggtgcgatg
     2941 tttttactga cgaaatcact gacattgatt tctttattcc aaagcccatt ggtaaaggtt
     3001 ttgttcaatt ccataatatt tgtttttgtt agtaggttag ataatgttat cctttagagc
     3061 gcgcaaaaat actgaataaa ttccgtaatt ttataagaga tacttctctt tttatgttga
     3121 acagcataaa ataactatta ttaggtagtg aatgtccggg ggagtagaga tgttaatact
     3181 ttctttatat gtggtttatg aattgaaaca agaaccgaat aataatctgc attatttaca
     3241 aatgatgtag gtttaaggaa cagtcgtatt ctgaacctaa ttcagtcgta tagtgaatgc
     3301 cattcagtcg tatactgaat agggttcaat cgtatactga atgtggctct ttgtaaatat
     3361 actgatagcc agtaagttat aaaactctgt cttgcccttg ataaatttca acaaatagcc
     3421 taaatgccgt tatgttcgtt ccgtagtggg tataaacaag aattttggta taattgctct
     3481 gtttttgtgc agaaaagttt gtagaataaa aaatgatttg tatctttgca ccgctttaaa
     3541 agaaaagtac ttctgaaaaa gaagttttgg agaggtggca gagtggtcga ttgcggcggt
     3601 cttgaaaacc gttgtgctgc gaggcacccg gggttcgaat ccctgtctct ccgcaataaa
     3661 catt
//
