LOCUS       c00279_gnlPro..         5446 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OBMANACO_279.
ACCESSION   c00279_gnlPro..
VERSION     c00279_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 06:22:58
            Original ID  :: gnl|Prokka|OBMANACO_279
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5446
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5446
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2353:5312]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2354..5312
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5446
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5446
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..71)
                     /locus_tag="ctg279_1"
                     /transl_table=11
                     /translation="MKKFNLITFAVIPVSYTHLRAHE"
     CDS             259..807
                     /locus_tag="ctg279_2"
                     /transl_table=11
                     /translation="MNELELSERCRQGDNRARKELYEQYAGRMLGVCLRYAGDRDMAQD
                     LVHDGFLKIFDSFDKFTWRGEGSLRAWMERVMVNTALQFLRKNDVMNQTTALDEVPETY
                     EEPDASAVEAIPQKVLMQFINELPAGYRTVFNLYTFEDKSHKEIAQMLGINEKSSASQL
                     FRAKSVLAKKVKEWLVTNG"
     CDS             862..2121
                     /locus_tag="ctg279_3"
                     /transl_table=11
                     /translation="MKEDEKWIKAFKDKLEDYSEPMPASGWERLERELMPVTEKRIYPY
                     RRWAVAAAAVVLVVTTAVSLYFLNSPVADEIRYATTPSLAINPDVLPEPALPDVQVAVS
                     EPVKPVGTTSINPVSGYLAKNTDPVIVPEVSSLAEKRPEAVTEEKRAEPQQEATAAIEK
                     KESATAQPPKRKEARRPSGKDKYQLPIGDSSAKRGGKWSMGVGIGNGGGLPTNGSEIFA
                     PRPMTNRVDLMTIMNGAVSIPADQEVIFEEGVPYLKSNTTAVMDYEHHQPVSFGLSVRK
                     SLPKGFSVETGLTYTLLSSDIKRQGDTKMQSQKLHYIGIPVRGNWNFLEKKYFTLYVSA
                     GGMVEKCVYGKLADDKVNVKPLQFSVAGAVGAQFNATDHVGLYVEPGVSYFFDDGSKVQ
                     TIRKERPCNFNLQAGLRFTY"
     CDS             complement(2354..3079)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.9;
                     E-value: 1e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg279_4"
                     /sec_met_domain="Radical_SAM (E-value: 2.5e-31, bitscore:
                     97.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIEAKGG
                     TATDPEEIVRMAVSQKAFFGKKGGITFSGGEPTFQAKSLIPLFKRLKEKGIHICLDTNG
                     GLWNNDVEELLELTDLVLLDIKEFNPEHHQSLTGRSNEQTLKTAAWLETNHKPFWLRYV
                     LVPGYSDFEDDIRQLGEHLGTYQMIQRVEILPYHTLGVHKYEAMNKEYMLKGVKENTPE
                     QIEKAEKLFRQYFRTVQVN"
     CDS             complement(3084..5312)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg279_5"
                     /sec_met_domain="Gly_radical (E-value: 1.8e-47, bitscore:
                     148.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1167.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFKDGLWSKEINVRDFVSNNITPYEGDASFLQGPTERTKA
                     VWNHCLKALEEERNNNGIRALDYTTVSTITSHPAGYIDKENELIVGLQTDQVLKRAIKP
                     FGGINVVMKACRENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGLDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKEIK
                     VLGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSQ
                     GTITETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKAFCAQVSIDTSSVQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKDIPVLTGETL
                     KFEEVMANYKKVLIQIARVYNEAMNIIHYMHDKYYYEKAQMAFVDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAHVKARRNDIGLTEGFDIEGAFPCFGNDDDRVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGIAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATQEDRIDNLVTMMDGYFTKGAHHLNVNVLN
                     REMLQDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 gtctcgtggg ctcggagatg tgtataagag acagggatga cggcaaaagt tattaaatta
       61 aatttcttca taagtttctt gttggtttta tcctatttat ccattgagat ttattcttta
      121 ttgtattgag aaaatgcagg aagtgatgaa atattgcatc ctaaaatgtt aaagaaacaa
      181 aagtgataaa agagttgttc tctctgtgca gtatttctat ctttgctgca tttataagat
      241 aaacacgtgt ttaggagaat gaatgaatta gagctgtcgg aacgttgcag gcagggagat
      301 aatcgtgccc gcaaagaact ttatgagcag tacgcagggc gcatgcttgg tgtctgtctt
      361 cgttatgccg gagacaggga catggcacaa gacttggtac acgacggttt cctgaagatt
      421 ttcgactcct tcgacaagtt tacctggcgg ggtgaaggct ctctgagagc atggatggag
      481 cgtgtaatgg tgaatacggc tttacagttc ttaagaaaga acgacgtgat gaaccagacc
      541 acggcactgg atgaagttcc cgaaacgtat gaagaaccgg atgcttcggc tgttgaagca
      601 ataccacaga aagtgctgat gcaatttatt aatgaactcc ccgccggata tcgtaccgta
      661 ttcaatttat acacctttga agataagtcg cacaaggaaa tcgcacagat gttggggatt
      721 aatgaaaaat cttctgcctc acagcttttt cgcgcaaaaa gtgtattggc aaagaaagtg
      781 aaagaatggt tggtgaccaa tggttgacat gtatttacag atactgaggt gaaccttgga
      841 tttcaaaaat gaaatagaat gatgaaagaa gacgaaaaat ggattaaagc attcaaggat
      901 aagctcgaag attattcgga gccgatgcct gcttccggtt gggaacgact ggaaagggaa
      961 ctgatgcctg taaccgaaaa acgaatatat ccgtatcgcc ggtgggcggt tgctgcggca
     1021 gctgtggtat tggtggtaac gactgctgtc agtttgtatt ttctgaatag tccggtagct
     1081 gatgaaatcc gttatgcaac gactccttca ctggctatca acccggatgt attgccggaa
     1141 cctgccttgc cggatgtaca ggtagctgta tccgaacccg ttaagccggt aggaacgacg
     1201 agcatcaacc ctgtttcggg ctatttggca aaaaatactg atccggtgat tgttccggag
     1261 gtatcctccc tggcagagaa gcgaccggag gcagtgacgg aggaaaagag agctgagccg
     1321 caacaggaag caactgctgc aatcgagaaa aaagaaagtg cgacggcgca gcctccaaag
     1381 aggaaagaag cgcggcgacc gtccggtaaa gacaagtatc agttgcctat cggagactct
     1441 tctgctaaac gtggtggtaa atggtcgatg ggagtgggta tcggtaatgg aggtggactt
     1501 ccgacaaatg gttccgaaat ttttgcgccc cgtccgatga ctaacagggt cgacttgatg
     1561 acgatcatga atggagctgt gagtattcct gccgatcagg aagttatatt tgaagaaggg
     1621 gttccctatc tgaagtccaa tacaactgcc gtgatggatt atgaacatca tcagccggtt
     1681 agcttcggac tgtctgtacg taagtctctt cccaaaggtt tctctgtaga gacgggattg
     1741 acttatacgt tgttgtcttc tgacatcaaa cggcaggggg ataccaagat gcagagtcag
     1801 aaattgcatt atataggtat tcctgtacgt ggaaactgga attttctgga aaagaaatat
     1861 tttacactct atgtatctgc cggaggaatg gttgaaaagt gtgtttatgg aaagttggcg
     1921 gatgataagg tgaatgtgaa gcctttgcaa ttttctgtag ccggtgccgt gggagcgcaa
     1981 ttcaatgcga cagatcatgt cggcctatat gtggaaccgg gagtttcgta tttctttgat
     2041 gatggctcca aagtacaaac catccgaaaa gaaaggcctt gtaactttaa cttgcaggca
     2101 ggattgcgat tcacttatta aagtagggtg gcaaaaaata tgccggttac attatctccc
     2161 tgttcttgcc ttgtattgta atgaattgag gcaagaacag ggagattttt atgtcgtgac
     2221 ttacaaaatt ataaataagc gcaatcgtca taattcaccg cagagtgaac ttaatctgtt
     2281 gattgccaat gaatctttat ctgtgtaaat tcgtgtaatc tgtggcgaat acgaatttcg
     2341 acacttatat aagtcaattc acttgtacgg tccggaagta ttgcctgaat agtttctcag
     2401 ccttttctat ctgctccgga gtattctctt tcactccttt cagcatatat tctttgttca
     2461 ttgcttcata tttgtgaaca cccaaggtgt ggtaaggcaa tatttctaca cgctgaatca
     2521 tctggtacgt tccaagatgc tctcccagtt gccggatatc gtcttcgaag tcactgtagc
     2581 cgggcactaa tacgtatcgc aaccagaatg gtttatgatt ggtttcaagc caggctgcgg
     2641 ttttcagggt ctgctcgttg cttcttccgg ttaaagactg atgatgttcc ggattaaatt
     2701 ctttgatatc taataaaacc agatcagtca attccaatag ttcctctaca tcattgttcc
     2761 ataacccgcc attggtatca aggcaaatat ggattccttt ttcctttagt cttttaaaaa
     2821 gtgggataag cgatttagct tgaaaagtag gctctccgcc ggaaaaggtg atacctccct
     2881 ttttaccgaa gaaagctttc tgactgacgg ccatgcgaac tatttcctca gggtcggttg
     2941 ctgtccctcc tttggcttca attgtgtccg gattggcaca atagaggcaa cggaagttac
     3001 acccttgaag gaaaacgacg agccggagac ccggcccgtc gaatgttccc attgactcgt
     3061 atgagtgtac gtttatcatt caattacatt ctttcgtgaa aactgcggct gattacttcc
     3121 aactgatgtt cgcggctcaa tttcacgaaa ttaacggcat atcctgagac acggatagtc
     3181 agttgcggat acttttcggg atgttccatc gcatcctgca acatttcccg gttcagcacg
     3241 tttacgttca ggtggtgtgc gccttttgta aagtatccgt ccatcatggt caccagattg
     3301 tcgatacggt cttcttgtgt tgctcccaat gatttgggaa cgatcgagaa tgtattgctg
     3361 ataccatcct gtgagtcgcg ataacgcaac ttggctacag aactcagtga ggcgattgca
     3421 ccatttttat cacgtccgtg catggggttg gcaccgggag caaaggcaat gcctttggcg
     3481 cgtccgtcag gagtggcacc ggtcttcttg ccatacatta cgttcgaagt gatagtcagc
     3541 agcgagaggg taggacgggc attcttatat acggggagtt tctttaattc ttcgctaaag
     3601 aaatatacca ggtccacacc caggtggtct acacggtcat cgtcattacc gaaacaaggg
     3661 aaagcacctt cgatatcgaa tccctccgtc agtccgatgt cgttgcgacg ggctttcaca
     3721 tgtgcatatt tgatggcgga cagtgaatcg atggcgattg aaagtccggc tacaccataa
     3781 gcgaggttaa tgcggggatc agtatccacg aaagccatct gtgccttttc gtagtaatac
     3841 ttatcgtgca tgtaatgtat gatattcatt gcttcgttgt atacacgtgc aatttgtatc
     3901 aatactttct tatagttggc cattacttct tcgaacttca gtgtttcacc ggtcaataca
     3961 gggatgtctt ttaccataac tgtacctgta ttttcgcaac gtcctccgtt gatagccagt
     4021 agcagggctt tggcaaggtt ggcacgtgcg ccgaagaact gaatctgctt gccgatttcc
     4081 tgatatgaaa cacaacaagc gattccgtag tcatccgatt gacggacttc acgcatcagg
     4141 tcgtcgtttt cgtattgtac ggaagaagtg tcgatagata cttgtgcaca gaatgctttg
     4201 aaaccttcgg gtaactcagg gctccatagt acagtcatgt taggttccgg tgacgggcct
     4261 aagttataaa gtgtctgcaa gaagcggaaa gaagttttag taactttggt acgtccgtca
     4321 ttgaaacgtc caccgataga ttcggtcacc catgtgggat cacccgcaaa aatatcgttg
     4381 tacgactgca tacgcaagtg gcgtaccata cggagtttga taacgaattg gtcgatcagt
     4441 tcctgagcga atgtttcggt tatcgtaccc tggcttaatt catattcaag atagatgtcg
     4501 aggaatgaag atacattacc cagtgacatg gcagcaccat cctgctcttt tacggcagcc
     4561 agataagcca tatacaccca ttgtacagct tcctgtgcgg tatatgccgg tcggctcaga
     4621 tccaaaccat aatattcgcc caatacttta atctctttca gggccttgat ttgctcggct
     4681 acttcttcac gaagacgaat gcgggcttca gtcataggac ctgtcagatt atgcagatct
     4741 tcttttttag cttctatcag acggtcaaga ccatagagag ccaggcggcg ataatcaccg
     4801 atgatacgtc cgcgtgcata gttgtcggga agtccggtaa ggaatcccaa tgaacggaac
     4861 gaacgaattt cttcggtata cacatcgaat accccatcat tatgagtctt gcgatagtga
     4921 gtgaagatat ccttaactct gtcatctact tccactccgt tttcacggca agctttcata
     4981 accacattga ttccgccaaa cggtttgatg gcacgtttta acacctgatc ggtctgtagt
     5041 ccgacgatca gttcgttctc tttatcgatg tagccggcag ggtgagaggt tatagtagat
     5101 acagttgtgt agtcgagtgc acgaatacca ttattgtttc tctcttcttc gagagctttg
     5161 agacagtgat tccagacagc tttggtgcgt tcggtaggtc cttgcaggaa agaagcatcc
     5221 ccttcataag gagtgatgtt gttactaacg aaatccctta cattgatttc tttgctccaa
     5281 agtccatctt taaaggtctt attcagttcc ataaaattaa tcagttagtg gttagttatc
     5341 ctttagagcg cgcaaattta caaccttttt tcggtttagg tatataaaag ggtcctattt
     5401 ctgtctctta tacacatctg acgctgccga cgatcttacg cgtgta
//
