LOCUS       c00377_gnlPro..         4205 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HBJAAMNO_377.
ACCESSION   c00377_gnlPro..
VERSION     c00377_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 05:38:25
            Original ID  :: gnl|Prokka|HBJAAMNO_377
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4205
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4205
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1178:4149]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1179..4149
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4205
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4205
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..523
                     /locus_tag="ctg377_1"
                     /transl_table=11
                     /translation="LVGSEMCIRDRSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEEL
                     CEAITSRSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEF
                     SGSMIKGAHLIFRPGKTLAELMKILSYQQVQTRAVAQAGAGDGGGEDPDKNPDSGGDGS
                     GDEEAPDPTV"
     CDS             583..750
                     /locus_tag="ctg377_2"
                     /transl_table=11
                     /translation="MKEIVSRILDVIMYLIPFFGKRKRDRVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             737..1186
                     /locus_tag="ctg377_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(1179..1907)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 71.7;
                     E-value: 2.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg377_4"
                     /sec_met_domain="Radical_SAM (E-value: 1.1e-29, bitscore:
                     92.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKTLVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(1921..4149)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg377_5"
                     /sec_met_domain="Gly_radical (E-value: 3.1e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     KFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 tctcgtgggc tcggagatgt gtataagaga cagatcttat tcagtggtgc cgcgcaagaa
       61 tcctgcaaaa aagagcgaac cggcaaagta ttatgcacag gcgcaggcgt cgggagagtt
      121 ggactttgaa gagttgtgcg aagcgatcac cagtcgttct acttgtacgg aaacggatgt
      181 acgtgcggct atttcgggta ttctttacga agtgaagcgt gcgttgaagg caggaagaat
      241 tgcgagactg ggcgatttgg gcagtttgca gataggactc aacagtgaag gtgcggcatc
      301 ggtcaaggaa ttttccggtt caatgattaa gggggcgcat cttattttcc gccccggtaa
      361 gacgctggcg gagttgatga agattctgag ttatcagcaa gtgcagactc gtgcggtggc
      421 gcaggcgggt gcaggcgatg gcgggggaga agaccctgac aagaatccgg attccggtgg
      481 tgatggctcc ggtgatgaag aggctccgga ccctacggta taattggcag acgggtcaag
      541 gtaataacga attagaataa cttccattaa tattttgata agatgaaaga aatagtaagt
      601 cggattttag atgtgatcat gtatctgatt cctttcttcg gaaagagaaa gcgggacagg
      661 gtggtgagag aagtgcgcta tcatactact tgtaaggagg tgtgcaaagt gaaaacgacg
      721 gaaagggaaa aggaacatga gaaagattag tctgattgtg atccattgtt ccgccacccg
      781 tgtcgattgc gactttacgg caaaggatgt ggatacggct caccgttatc gtggcttttc
      841 gtgctggggg tatcattatt atatacgcaa gtcgggcgag attgagccga tgcgggatga
      901 agatacggtc ggagctcatg cacgtggcta taatgcgatc agcttaggtg tatgttatga
      961 gggcggtctg gatgagaatg gaaaagctgc ggatacgcgt acaccccgtc agaaggaggc
     1021 gctacatcgc ttggtacacg aattgttgca gcgttatccg gaagctaaag tagtagggca
     1081 tcgggattta agtcccgaca ccaactataa tgggatcgta gatccttggg aacggattaa
     1141 agaatgccct tgctttgagg tcattggaga attctccatt aattaactac caccgtagtg
     1201 aaatactctt tgaatacctc cgcagctttt tcaagctgtt cgggggtatt ttcttttacc
     1261 cctttcatct tgtattcctg tcccatcgct tcgtacttat gtacgcccag tgtatggtaa
     1321 ggcagtattt ccacccgttg gatcatcttg tattttccga gtgcttctcc cagtgcgcgt
     1381 atgtcttctt caaaatcgct gtatccgggc actagcacgt agcgtagcca gaaaggtttc
     1441 tcctgttctt ccagccaggc ggccgtgcgg atggtttgct cgttgcttct tccggtgagc
     1501 gtctgatggc ggttggggtt gaattccttt atatccaata acacaagatc ggtcagtctg
     1561 aataattctt ctacgtcttc gttccaaagt cctccgttgc tgtccagaca gacatggatg
     1621 cctttttctt tcagttcgcg gaccagcggg acgagtgttt tcgcctgaaa cgtcggttcg
     1681 cctccggaga aagtgattcc tcctcgtttc ccgaagaagg ggcgctggct cattgccatg
     1741 cggacgattt cttccggtgg ggtaggcgtg cctccctttc ctgctatcgt atccggattg
     1801 gcgcaataga ggcagcggaa gttgcatcct tgaagaaaaa cgacgagccg taagcccggc
     1861 ccgtcgaatg ttcccatact ttcgtatgaa tgtacgttta tcatcatata gagtaataga
     1921 ttacatacgt tcgtggaagc tacggctgat aacttccagt tggtgttcgc ggctcagctt
     1981 cacgaagttt acggcatagc cggaaacgcg gatagtcagc tgcggatagt tttccggatg
     2041 ctccatggca tcgtacagca tatcacggtt cagtacgttt acgttcaggt ggtgagcacc
     2101 tttggtgaag taaccgtcca tcatcgttac gaggttctcg atacggtctt cgtccgtagc
     2161 acccagtgat ttcggaacga tagagaaggt gttgctgata ccgtcctgtg agtcacggta
     2221 acgaagtttc gctacagagc tcagagaagc gatcgcacca ttcttgtcgc gtccgtgcat
     2281 cgggttggca cccggagcga aggcaacacc tttggcacgt ccgtcgggag tagcaccggt
     2341 ctttttgccg tacatcacat tggaagtgat agtcagcaag gagagggtag gacgggcatt
     2401 cttgtaaact ggcaatttct tcaattcttc gctgaagaag tataccaggt ctacgccaag
     2461 gtggtctacc ttgtcgttgt cgttaccgaa gcaagggaat tctccttcga tgtcgaaacc
     2521 ttctgtcagg ccgatatcgt tgcggcgggc agttactttc gcatatttga tggcagacag
     2581 tgaatcgagg gcgatggaaa gtccggctac accgtaagcc aggttgatgc gcgggttcgt
     2641 atctacaaga gccatctggg ccttctcgta gtaatacttg tcgtgcatat agtggatgat
     2701 gttcatcgct tcgttatata cgcgggcgat ttcggtcagc acttttttgt agttgtccat
     2761 tacttcttcg aacttcagcg tgtcactggt cagtacgggg atgttcttca ccatgactgt
     2821 accggtattc tcgcaacgtc cgccgttgat ggcaagcaac agggctttgg ccaggttgca
     2881 acgggcaccg aagaactgaa tctgcttgcc tatttcctgg tatgatacgc agcaggcgat
     2941 tccgtagtcg tcggactgac gtacttcacg catcaggtcg tcgttttcgt actggataga
     3001 agaagtgtcg atagaaacct ttgcacagaa ttctttgaat ccttccggaa gttccgggct
     3061 ccagagtacg gtcaggttcg gttccggtga agggccgagg ttgtacaatg tctgcagaaa
     3121 acggaaggag gtctttgtca ctttggtacg tccgtcgttg agacgtccgc ccagagattc
     3181 ggttacccag gtcgggtcgc ctgcaaagat gtcgttgtat gattgcatac gcaggtggcg
     3241 gaccatacgc agtttgatga caaactggtc gatcagctct tgcgcgaacg attcggtgat
     3301 ggttcctttg ctcagttcat attccagata gatatcgagg aaagaagaaa cgttacccag
     3361 tgacatagcg gcaccgtctt gttctttgac ggcggcaagg taagccatat atacccactg
     3421 tacggcttct tgtgccgtgt aagcgggacg gctcaggtcg agaccgtagt attcgcccat
     3481 cactttcatg tctttcagtg ccttgatctg ttctgccact tcttcgcgca ggcggatgcg
     3541 ggcctctgtc atcggaccgg tgaggttgtg caaatcttcc ttctttgctt cgatcagacg
     3601 gtcgatgccg taaagagcca tacggcggta gtcaccgatg atgcgtccgc gtgcataatt
     3661 gtcgggaagt ccggtaagga atcccagcga acggaaggaa cggatttctt ccgtatatac
     3721 gtcgaatact ccgtcgttgt gcgtcttgcg gtagtgagtg aaaatatctt ttacgcggtc
     3781 gtccacttcc acgccgtttt cgtgacaggc tttgctgact acgttgatac ctccgaaagg
     3841 cttgatggca cgtttcagaa gttcgtctgt ctgcaggccg acgatcagtt cgttttcctt
     3901 gtcgatatat ccggccttgt gggaagtgat ggttgatacg gtaacattat ctaatgagcg
     3961 gacaccgttg ttttctcttt cttctgccag tgcttcgagg cagcggttcc atacggcttt
     4021 ggtacgttct gtaggtcctt cgaggaatga agcatctccg taatacggag tgatgttatg
     4081 acttacgaaa tctctgacgt tgatttcgct gctccaaaga ccgtctttaa agatcttgtt
     4141 taattccata aaatgataaa attagaggtt gtagttatcc ttttctttag agcgcacaaa
     4201 gttac
//
