LOCUS       c00312_gnlPro..         4793 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|POPCJFKF_312.
ACCESSION   c00312_gnlPro..
VERSION     c00312_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 01:57:11
            Original ID  :: gnl|Prokka|POPCJFKF_312
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4793
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4793
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[212:4793]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      213..4793
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..4793
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4793
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(213..1373)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg312_1"
                     /sec_met_domain="OAD_beta (E-value: 2.8e-154, bitscore:
                     501.6, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDFINFLGNNLADFWTYTGFANATVGHVVMILVGLVFIYLAIAK
                     EFEPMLLIPIGFGILIGNIPFNMDAGLKVGIYEEGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYMIALEMGFDPMQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPVIQPPIMRLLTTKNERVIRMKPPRAVSHTEKVIFP
                     IIGLLLTCFLVPSGLPLLGMLFFGNLLKESGVTRRLANTASGPLIDTITILLGLTVGAS
                     TQASEFLTLDSIKIFALGALSFVIATASGVIFVKIFNIFLKKGNKINPLIGNAGVSAVP
                     DSARISQVIGLEYDSTNYLLMHAMGPNVAGVIGSAVAAGILLGFLM"
     CDS             complement(1375..1806)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 67.7; E-value:
                     2e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg312_2"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1.6e-22, bitscore:
                     67.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEYKYKINGNSYKVTIGDIEDNIAHVEVNGTHYKVEMEKQPKPV
                     AKPVTVRPMPNAPAAPTQVVKPTAPSTGKSGVKSPLPGVILDIKVNVGDTVKKGQTIII
                     LEAMKMENNINADKDGKITAINVNKGDSVLEGNDLVIIE"
     CDS             complement(1831..2751)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg312_3"
                     /sec_met_domain="OAD_gamma (E-value: 3e-16, bitscore: 48.4,
                     seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTKIGIFLSLLLLIGLTSCGEQKSNNKLVLNEILIDNQSNFQD
                     DYGLHSAWIEIFNKSFGSADLAACLLKVSSQPGDTVTYFIPKGDILTLVKPRQHALFWA
                     DGEPNRGTFHTSFKLNPETANWVGLFDSGKKLLDQIVVPAGALGPNQSYARVSDGAAEW
                     EVKSGSGDKYVTPSTNNKTLDSNSKMEKFEEHDADGIGMSISAMSVVFCGLILLFIAFK
                     IVGKVAVNLSKRNAMKSKGIDKHEAKELSQAPGEVYAAISMALHEMQDEVHDVEETVLT
                     ITRVKRSYSPWSSKIYTLRETPPRK"
     CDS             complement(2783..4336)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg312_4"
                     /sec_met_domain="CT_Btheta (E-value: 8.1e-291, bitscore:
                     952.9, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKIKELIERRAVARIGGGEKAIAKQHEKGKYTARERLAML
                     LDEGSFEEMDMFVEHRCTNFGMEKKHYPGDGVVTGCGTIEGRLVYVFAQDFTVSAGSLS
                     ETMSLKICKIMDQAMKMGAPCIGINDSGGARIQEGINALAGYAEIFQRNILASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMMEGTSYMFLTGPKVVKTVTGEDVSQENLGGASVHST
                     KSGVTHFTAKTEEEGFELIRKLLSYIPQNNLEEAPYVDCTDPIDRLEDSLNDIIPDSPT
                     KPYDMYEVIGAIVDNGEFLEIQKDYAKNIIIGFARFNGQSVGIVANQPKYLAGVLDSNA
                     SRKGARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTI
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVEVLYAREAKDQENPAQFLA
                     EKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRVIRALQQLQTKKLSNPAKKHGNIPL
                     "
     CDS             complement(4365..4793)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg312_5"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.3e-24, bitscore:
                     75.2, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DPLWSRGLGDVYKRQHLGIAVKSIEEALPYYENVLGLKCYNIETV
                     EDQKVRTAFLKVGETKIELLEPTCPESTIAKFIENKGAGVHHVAFAVEDGVANALAEAE
                     GKEIRLIDKAPRKGAEGLNIAFLHPKSTLGVLTELCEH"
ORIGIN
        1 gaaatgataa gagaagataa cacaacggaa cgcgatacga cgcatacgcc atagcaaatg
       61 caatattttt taggcggtaa gttcttacaa taaaaaatca cctttactac aatgatgcag
      121 taaaggtgat tttataaatt caaggctttt aggataatct tattttttgt tttaagaact
      181 gttcaaacaa gcgtcccctc ttgccttata tcttacatca agaatccaag caggataccg
      241 gcagcaacag ctgaaccgat cactccggca acgttcggac ccatagcgtg catcagcaaa
      301 tagttggtcg aatcatattc caaaccaatc acttgcgaga tacgtgcaga gtcgggaaca
      361 gcagaaacac ctgcattacc gatcaacgga ttgatcttat tacccttctt caggaagata
      421 ttgaagatct taacgaagat gacaccggat gccgttgcaa taacaaatga caatgcaccg
      481 agggcaaaga tcttgataga gtcaagcgtc aggaattcgg atgcttgtgt agaagcacct
      541 actgtcagac ccaacaggat agtgattgta tcaatcaacg gaccactggc agtattagcc
      601 aaacggcggg ttacaccact ttctttcaac aggttaccga agaacagcat acccagcaaa
      661 ggcagaccgg aaggaaccag gaaacaagtc agcaacaaac cgatgatcgg gaaaatcacc
      721 ttctccgtgt gagaaacggc acgtggcggt ttcatgcgga tgacacgttc gttcttggtg
      781 gtgagcagac gcatgatagg cggctgtatc accggaacta acgccatata ggagtaggcg
      841 gacaccgcaa tggctcccat caggttaggt gctagcttgg aagaaaggaa gatggccgtc
      901 gggccgtctg ctccaccaat gataccgata gcaccggctt gcatcggatc gaagcccatt
      961 tctaacgcga tcatatatgc accgaagata ccgaactgtg cagctgcacc aattagcatc
     1021 aattttggat tagagataag tgccgagaag tccgtcatgg caccgatacc caaaaagatg
     1081 agtggcggat accagccgga ggtcactccc tgatacaata tattcaatac agaaccttct
     1141 tcataaatac cgactttcag cccggcatcc atattaaaag gtatgttacc gatcaatata
     1201 ccgaaaccga taggaatcag cagcatcggt tcgaactctt tggctattgc caaatagatg
     1261 aataccaagc caacgagaat catgactaca tgccctaccg tagcattggc aaagcctgtg
     1321 tatgtccaga agtcggccag gttatttcct aaaaagttta taaagtctcc catattattc
     1381 aataattacg aggtcattac cttcaagaac agagtctccc ttgttgacgt tgatggcagt
     1441 aatcttaccg tccttatccg cattgatatt gttttccatc ttcatggctt ccaatatgat
     1501 aatggtctgt cctttcttta cagtatcgcc tacattcact ttgatgtcga ggatcacacc
     1561 cggcagcgga gattttactc ctgattttcc ggtagacgga gcggtcggtt tcactacttg
     1621 agtaggagca gcaggagcat taggcatcgg gcgtactgtc acgggtttcg caacaggctt
     1681 cggctgtttc tccatttcta ctttatagtg tgtaccgttc acttctacat gagcgatgtt
     1741 gtcttcaata tctccgatgg ttactttata tgagttaccg ttgattttat atttatattc
     1801 tttcatcgtt tttacttttt aaaaggtgac ttactttctg ggaggagttt cacgcaaagt
     1861 gtaaatcttc gaactccatg gtgagtagct gcgttttacg cgggtgatgg tcagcaccgt
     1921 ttcttctacg tcgtgcacct catcctgcat ttcgtgtaat gccatagaaa tagcagcgta
     1981 tacttcgccc ggagcctgtg acagttcctt agcttcatgc ttgtcaatgc ctttagattt
     2041 catagcgttg cgcttgctca aattaacagc tacttttcct acaatcttaa aagcaataaa
     2101 gagaagaatc aatccgcaga atactacgct catggcagaa atggacattc caatgccatc
     2161 agcatcgtgt tcttcaaact tttccatctt ggaattgctg tcaagagtct tgttgttggt
     2221 gctcggagtc acgtatttat caccgcttcc actttttact tcccattcag ctgccccgtc
     2281 gcttacacga gcgtatgatt ggttgggacc gagcgcgcct gcgggcacta cgatctggtc
     2341 gagcaacttc ttgccggagt cgaacagacc tacccagtta gctgtttccg gattcagctt
     2401 gaagctggta tggaaagtac cacggttagg ttcgccatct gcccagaata gtgcatgttg
     2461 gcggggtttc actaaggtaa gtatatcacc tttcgggata aaataagtaa ctgtatcgcc
     2521 cggttggctg cttactttca gcaagcaagc tgccaggtcg gcgctaccga atgatttatt
     2581 gaatatttca atccatgcgc tgtgcaatcc gtaatcgtcc tgaaaattac tctgattgtc
     2641 tatcaggatt tcgtttagca ccagcttatt gttcgacttt tgctctccac aagaagtcaa
     2701 accaatcagc aacagcaaag aaaggaatat tccgattttg gttttgttca taatcgttct
     2761 tgttttaatg tgaaaagtag gattacaacg gaatattacc atgcttctta gccggattac
     2821 tcaatttctt ggtctgcaac tgctgcaaag ctcggataac acggaaacgt gtgttgcgcg
     2881 gttcaatcac atcgtcgatg tagccatatt tagctgcatt gtaaggattg gcgaacagtt
     2941 tcgtgtattc tgcttctttc tctgcaagga attgagcagg gttttcctga tctttggctt
     3001 cgcgtgcgta caatacttct actgctcctg caccacccat tactgcgatt tcggcagtag
     3061 gccatgcgta gttcatgtca ccgcgaagtt gcttacagct cattacgatg tgagaaccac
     3121 cgtaagattt acgcaatgta atagtaacct taggcacagt agcttcaccg taagcataca
     3181 acagtttagc accatgaagg ataacgccat tgtattcctg acctgttccc ggaaggaatc
     3241 ccggtacgtc taccaacgat acgataggaa tattgaatgc atcgcagaaa cgaacgaaac
     3301 gtgcaccctt acgagatgcg ttgctatcga gcacaccagc cagatactta ggctgattag
     3361 caacgatacc taccgattgg ccgttgaagc gggcgaaacc gatgatgata ttctttgcat
     3421 aatctttctg gatttcaagg aattcaccgt tgtccacaat agcgccgatc acttcgtaca
     3481 tgtcatacgg tttagtaggg ctgtcgggga taatatcgtt caaagaatct tccagacggt
     3541 cgattgggtc tgtgcaatct acataaggag cttcttcaag gttgttttga ggaatataac
     3601 tcaacagttt acgaatcagt tcgaaacctt cttcttctgt tttagcggtg aaatgagtca
     3661 cacctgattt agtggagtga acgcttgcgc caccgaggtt ttcctggcta acgtcttcac
     3721 ctgttacggt cttcacgact tttggtccgg taaggaacat ataagaagta ccttccatca
     3781 ttagcgtgaa gtcggtcaga gccggagaat aaacagcacc gccggcacaa ggaccgaaga
     3841 tacctgaaat ctgtgggata acaccggaag caaggatatt gcgttggaag atttctgcat
     3901 aacctgccaa agcgttgata ccttcctgaa tacgtgcacc acccgagtcg ttgataccga
     3961 tacagggagc gcccatcttc atggcttgat ccatgatttt acaaattttc aatgacatcg
     4021 tttctgacag tgaaccggca gaaacagtga agtcttgtgc gaacacatat accagacgtc
     4081 cttcgatcgt accgcagccg gttactacac cgtctcccgg gtaatgtttt ttctccatgc
     4141 cgaagttcgt gcatctgtgc tcaacgaaca tgtccatttc ttcgaagcta ccctcgtcaa
     4201 gcagcatagc cagacgctcg cgtgctgtgt atttcccttt ttcgtgttgc ttcgcaattg
     4261 ctttttcacc gcctccgata cgtgctacgg cacggcgttc aataagctct ttaatttttt
     4321 caagttgatt actcatgagt tcttgttggt tttagagttt atgattaatg ttcgcagagt
     4381 tctgttaaca cacccaaagt tgatttcggg tgaaggaaag cgatattcaa accttcagca
     4441 cctttgcggg gagctttgtc gataaggcgg atttctttgc cttctgcctc tgccagcgca
     4501 ttggctacgc catcttctac agcaaatgcg acgtggtgaa cgcctgcacc tttgttttca
     4561 atgaatttgg caatagtact ctccgggcaa gtaggctcca acagttcaat ttttgtttcg
     4621 cccactttta aaaaagcagt tcttactttc tgatcttcca ctgtttcaat gttgtaacac
     4681 ttcagaccta atacattttc gtagtaagga agggcttctt cgatgctttt tacagcaatg
     4741 cccagatgct gtctcttata cacatctccg agcccacgag accagagagg atc
//
