LOCUS       c00369_gnlPro..         4305 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|POPCJFKF_369.
ACCESSION   c00369_gnlPro..
VERSION     c00369_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 01:57:11
            Original ID  :: gnl|Prokka|POPCJFKF_369
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4305
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4305
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1198:4171]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1199..4171
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4305
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4305
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             50..553
                     /locus_tag="ctg369_1"
                     /transl_table=11
                     /translation="MAMTVTYSVVPRKNPAKKDESAKYYAQAQASGELDFEELCEGITS
                     RSTCTETDVRAAISGILYEAKRALKAGRIVRLGDLGSLQIGLNSEGAVSVKEFSSSLIT
                     AAHIIFRPGKTLADITKILSYQQVTTRAVAQTGGSGNEGEDDDKGSGGGSDGGGSGEAP
                     DPAA"
     CDS             574..741
                     /locus_tag="ctg369_2"
                     /transl_table=11
                     /translation="MKEIVTKILDVIMFLVPFFGKRKRNRIVREVRFNATHKEVCNVKT
                     TEREKDDEKD"
     CDS             728..1171
                     /locus_tag="ctg369_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRADRDFTAKDVDTAHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDEDGKAADTRTSCQKESLHRLVRELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVKAETW"
     CDS             complement(1199..1927)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg369_4"
                     /sec_met_domain="Radical_SAM (E-value: 2.3e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(1943..4171)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg369_5"
                     /sec_met_domain="Gly_radical (E-value: 2.6e-47, bitscore:
                     148.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1172.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLRNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSSELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     NFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             complement(4183..4305)
                     /locus_tag="ctg369_6"
                     /transl_table=11
                     /translation="EILSGLVGSEMCIRDRHIYADYMKKRCNFVRSKEKDNYNL"
ORIGIN
        1 caaataagga acgcgttcga aatgtatgaa aatttaaaat tattttatta tggcaatgac
       61 agtaacttat tcagtagtgc cgcgcaagaa tcctgccaaa aaggatgagt cggcaaagta
      121 ttacgcgcag gcacaggctt cgggagagtt ggattttgaa gagttgtgcg agggaatcac
      181 cagtcggtct acctgtacgg agacagatgt gcgtgccgcc atttcgggta ttctttatga
      241 ggcgaagcgt gcgttgaaag cgggaagaat agtccggctg ggtgatttgg gcagtttgca
      301 gatcgggttg aacagtgaag gagctgtgtc ggtcaaggag ttttcaagtt cgctgattac
      361 ggctgcccat attattttcc gtcccggaaa gacgttggcg gacatcacga agattcttag
      421 ctaccagcaa gtaacgacgc gtgcggtggc ccaaacgggc ggtagcggca atgaaggaga
      481 agacgatgac aaaggttccg gtggcggctc agacggtggt ggaagcggag aagctccgga
      541 tccggcagca tgataacttt aatattagat caaatgaaag aaattgtaac taagattttg
      601 gatgtaatca tgttccttgt gcctttcttc ggaaaaagaa agcggaacag aattgtgaga
      661 gaggtgcgct tcaatgctac ccataaggaa gtgtgtaatg tgaaaacaac ggaaagggag
      721 aaggacgatg agaaagatta gtctgattgt gattcactgc tctgctaccc gtgctgatcg
      781 cgacttcacg gcgaaggatg tggacactgc ccatcgcttt cggggctttt cttgttgggg
      841 gtatcattat tatatccgca agtcgggaca gatagaaccg atgagggatg aagatacggt
      901 gggtgctcat gcacgcggtt ttaatgcgat tagtttaggg gtgtgctatg aaggcggact
      961 ggatgaggac ggaaaggcag cggatacacg tacttcctgc cagaaagaat ctttgcatcg
     1021 cctggtgcgc gaactgttgc agcgttatcc ggatgcaaag gtggtcggtc atcgggactt
     1081 gagtcctgac accaattata atggaattgt tgacccttgg gagcggatca aagagtgtcc
     1141 ctgtttcgag gtgaaagcag agacatggtg atttttatcc tatttattac tgtggtggtt
     1201 aattgaccac cacagtagtg aaatactctt taaatacctc cgcggctttt tcaagttgtt
     1261 cgggggtatt ttcttttacg tctttcagct tgtattcctg ctccatcgct tcatacttat
     1321 gtacgcctaa tgtatgataa ggcaggattt ccactcgttg caccatctta tattttccca
     1381 gagcctctcc caagcggcgg atatcctctt cgaaatcgct gtatccgggc actaatacat
     1441 aacgcagcca gaaaggtttc tcgttttctt ccagccatgc tgccgtgcgg atggtttgct
     1501 cgttacttct tccggtcagt gcttgatggc gtgccggatt gaactcttta atatccagca
     1561 atacaagatc cgtcagtttg aaaagttctt ctacttcttc gttccagata ccgccgttgc
     1621 tgtctatgca tacatgtatt cccttttctt tcagttcacg cactaaaggg actagtgctt
     1681 tggcctgaaa cgtaggttct cctccggaga aagtgactcc tccgcgtttc ccgaaaaaag
     1741 gacgttggct catagccatg cggacgattt cttccggtgg ggtaggtgtg cctccttttc
     1801 cggctatcgt gtcggggttg gcacaataca ggcagcggaa attgcatcct tgaagaaaaa
     1861 cgacgagccg taagcccggc ccgtcgaatg ttcccatact ttcgtatgaa tgtacgttta
     1921 tcgtcatatt gagtagatct gattacatac gttcgtggaa gctacggctg ataacttcca
     1981 attgatgttc gcggctcaac ttcacgaagt ttacggcata accggaaaca cggatggtga
     2041 gttgcggata tttttccgga tgttccatgg catcgtaaag catttcacgg ttcagtacgt
     2101 tcacgttcag gtggtgagcg cctttggtga aatagccatc catcattgtc accagatttt
     2161 ctacgcgatc ctcttcagta gctcccagtg atttggggac gatggagaaa gtgttgctga
     2221 taccgtcctg tgagtcgcgg taacgtaatt tcgctacgga actcaaagag gcgatggcac
     2281 cgttcttgtc acgtccgtgc atagggttgg ctcccggagc aaaagcgaca cctttggcgc
     2341 gtccgtcggg agtagcacct gtcttcttgc catacatcac gttggaagtg atggtgagca
     2401 aagataaagt aggacgggca tttttgtaaa ccggaagttt cttcaattct tcgctgaaga
     2461 aatagaccag gtcaacaccc aggtggtcta ccttgtcgtt gtcattaccg aagcaaggga
     2521 attcgccttg aatatcgaaa ccttctgtca gaccgatgtc gttgcggcgt gcggtcactt
     2581 tggcatattt gatggccgac agtgaatcga gggcgataga aagtccggct acaccataag
     2641 caaggttgat gcgtgggttg gtatctacaa gagccatttg ggctttttcg tagtaatact
     2701 tatcatgcat ataatggatg atgttcatcg cttcgttgta aacacgggcg atttctgtca
     2761 ataccttctt gtagttgctc attacttctt cgaagttcag tgtgtcgctg gttagtacgg
     2821 ggatattttt caccattacc gtaccggtgt tttcgcaacg tccgccgttg atggcaagca
     2881 gcagggcttt agccaggttg caacgtgcgc cgaagaactg gatttgtttt ccgatttcct
     2941 gataagatac gcagcacgcg attccgtagt cgtcagactg gcgtacttca cgcatcaggt
     3001 cgtcgttttc atattggatg gaagaagtgt cgatagatac ttttgcgcag aattctttga
     3061 atccttccgg cagttccgag ctccacaata cagtcaggtt cggttccggt gaagggccga
     3121 ggttgtagag tgtttgcagg aagcggaaag aagtctttgt caccttggta cgtccgtcgt
     3181 tgaggcgtcc gcccagagat tcggttaccc atgtcgggtc gccggcaaag atgtcgttgt
     3241 aagattgcat acgcaggtgg cgcaccatac gcagtttgat gacaaactgg tcgatcagct
     3301 cctgtgcgaa agattctgtg atggttcctt tgctcaattc atattccata taaatgtcga
     3361 ggaacgaaga gacgttaccc agtgacatgg cagcaccgtc ttgttctttt acggcagcaa
     3421 ggtaagccat gtacacccat tgtactgctt cttgtgcggt gtaggcagga cggctcaagt
     3481 cgagtccgta gtattcgccc attaccttca tgtccttcaa tgccttgatt tgctctgcca
     3541 cttcttcgcg caggcggatg cgggcttcgg tcatcggacc ggtaaggtta cgcaaatctt
     3601 ctttttttgc ctcaatcagt cggtcgatac catagagagc catacggcgg tagtcgccga
     3661 tgatacgtcc gcgggcatag ttgtcaggaa gtccggtgag gaatcccaga gaacggaacg
     3721 aacggatttc ttcggtatat acgtcaaata ctccgtcgtt gtgcgtcttg cggtagtgag
     3781 tgaatatatc ttttacacgg tcgtccactt ccacgccgtt ttcgtggcag gctttgctta
     3841 ccacattgat acctccgaaa ggcttgatgg cacgtttcag aagttcgtct gtctgcagac
     3901 cgacgatcag ttcgttttct ttgtcgatat atccggcttt atgagaagtg atggttgaga
     3961 cggtgacatt atccagtgag cgtacgccgt tattggctct ttcttctgcc agtgcttcga
     4021 ggcagcggtt ccatacagct tttgtgcgtt cggtaggtcc ttcgaggaaa gaagcatctc
     4081 cataatacgg agtgatgttg tgactaacga aatctcttac gttgatttct gtgctccaaa
     4141 gaccgtcttt aaagatctta tttaattcca taaatgataa aattagaggt tgtagttatc
     4201 cttttcttta gagcgcacaa agttacaacg ttttttcata taatcagcat aaatatgcct
     4261 gtctcttata cacatctccg agcccacgag accagagagg atctc
//
