LOCUS       c00387_gnlPro..         3661 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KOAMHLGD_387.
ACCESSION   c00387_gnlPro..
VERSION     c00387_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 00:28:09
            Original ID  :: gnl|Prokka|KOAMHLGD_387
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3661
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3661
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[634:3605]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      635..3605
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3661
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3661
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             193..642
                     /locus_tag="ctg387_1"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(635..1363)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 71.7;
                     E-value: 2.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg387_2"
                     /sec_met_domain="Radical_SAM (E-value: 6.7e-30, bitscore:
                     92.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKTLVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(1377..3605)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg387_3"
                     /sec_met_domain="Gly_radical (E-value: 1.9e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     KFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 ctacacccta gagttcgtcg gcagcgtcag atgtgtataa gagacagata gtaagtcgga
       61 ttttagatgt gatcatgtat ctgattcctt tcttcggaaa gagaaagcgg gacagggtgg
      121 tgagagaagt gcgctatcat actacttgta aggaggtgtg caaagtgaaa acgacggaaa
      181 gggaaaagga acatgagaaa gattagtctg attgtgatcc attgttccgc cacccgtgtc
      241 gattgcgact ttacggcaaa ggatgtggat acggctcacc gttatcgtgg cttttcgtgc
      301 tgggggtatc attattatat acgcaagtcg ggcgagattg agccgatgcg ggatgaagat
      361 acggtcggag ctcatgcacg tggctataat gcgatcagct taggtgtatg ttatgagggc
      421 ggtctggatg agaatggaaa agctgcggat acgcgtacac cccgtcagaa ggaggcgcta
      481 catcgcttgg tacacgaatt gttgcagcgt tatccggaag ctaaagtagt agggcatcgg
      541 gatttaagtc ccgacaccaa ctataatggg atcgtagatc cttgggaacg gattaaagaa
      601 tgcccttgct ttgaggtcat tggagaattc tccattaatt aactaccacc gtagtgaaat
      661 actctttgaa tacctccgca gctttttcaa gctgttcggg ggtattttct tttacccctt
      721 tcatcttgta ttcctgtccc atcgcttcgt acttatgtac gcccagtgta tggtaaggca
      781 gtatttccac ccgttggatc atcttgtatt ttccgagtgc ttctcccagt gcgcgtatgt
      841 cttcttcaaa atcgctgtat ccgggcacta gcacgtagcg tagccagaaa ggtttctcct
      901 gttcttccag ccaggcggcc gtgcggatgg tttgctcgtt gcttcttccg gtgagcgtct
      961 gatggcggtt ggggttgaat tcctttatat ccaataacac aagatcggtc agtctgaata
     1021 attcttctac gtcttcgttc caaagtcctc cgttgctgtc cagacagaca tggatgcctt
     1081 tttctttcag ttcgcggacc agcgggacga gtgttttcgc ctgaaacgtc ggttcgcctc
     1141 cggagaaagt gattcctcct cgtttcccga agaaggggcg ctggctcatt gccatgcgga
     1201 cgatttcttc cggtggggta ggcgtgcctc cctttcctgc tatcgtatcc ggattggcgc
     1261 aatagaggca gcggaagttg catccttgaa gaaaaacgac gagccgtaag cccggcccgt
     1321 cgaatgttcc catactttcg tatgaatgta cgtttatcat catatagagt aatagattac
     1381 atacgttcgt ggaagctacg gctgataact tccagttggt gttcgcggct cagcttcacg
     1441 aagtttacgg catagccgga aacgcggata gtcagctgcg gatagttttc cggatgctcc
     1501 atggcatcgt acagcatatc acggttcagt acgtttacgt tcaggtggtg agcacctttg
     1561 gtgaagtaac cgtccatcat cgttacgagg ttctcgatac ggtcttcgtc cgtagcaccc
     1621 agtgatttcg gaacgataga gaaggtgttg ctgataccgt cctgtgagtc acggtaacga
     1681 agtttcgcta cagagctcag agaagcgatc gcaccattct tgtcgcgtcc gtgcatcggg
     1741 ttggcacccg gagcgaaggc aacacctttg gcacgtccgt cgggagtagc accggtcttt
     1801 ttgccgtaca tcacattgga agtgatagtc agcaaggaga gggtaggacg ggcattcttg
     1861 taaactggca atttcttcaa ttcttcgctg aagaagtata ccaggtctac gccaaggtgg
     1921 tctaccttgt cgttgtcgtt accgaagcaa gggaattctc cttcgatgtc gaaaccttct
     1981 gtcaggccga tatcgttgcg gcgggcagtt actttcgcat atttgatggc agacagtgaa
     2041 tcgagggcga tggaaagtcc ggctacaccg taagccaggt tgatgcgcgg gttcgtatct
     2101 acaagagcca tctgggcctt ctcgtagtaa tacttgtcgt gcatatagtg gatgatgttc
     2161 atcgcttcgt tatatacgcg ggcgatttcg gtcagcactt ttttgtagtt gtccattact
     2221 tcttcgaact tcagcgtgtc actggtcagt acggggatgt tcttcaccat gactgtaccg
     2281 gtattctcgc aacgtccgcc gttgatggca agcaacaggg ctttggccag gttgcaacgg
     2341 gcaccgaaga actgaatctg cttgcctatt tcctggtatg atacgcagca ggcgattccg
     2401 tagtcgtcgg actgacgtac ttcacgcatc aggtcgtcgt tttcgtactg gatagaagaa
     2461 gtgtcgatag aaacctttgc acagaattct ttgaatcctt ccggaagttc cgggctccag
     2521 agtacggtca ggttcggttc cggtgaaggg ccgaggttgt acaatgtctg cagaaaacgg
     2581 aaggaggtct ttgtcacttt ggtacgtccg tcgttgagac gtccgcccag agattcggtt
     2641 acccaggtcg ggtcgcctgc aaagatgtcg ttgtatgatt gcatacgcag gtggcggacc
     2701 atacgcagtt tgatgacaaa ctggtcgatc agctcttgcg cgaacgattc ggtgatggtt
     2761 cctttgctca gttcatattc cagatagata tcgaggaaag aagaaacgtt acccagtgac
     2821 atagcggcac cgtcttgttc tttgacggcg gcaaggtaag ccatatatac ccactgtacg
     2881 gcttcttgtg ccgtgtaagc gggacggctc aggtcgagac cgtagtattc gcccatcact
     2941 ttcatgtctt tcagtgcctt gatctgttct gccacttctt cgcgcaggcg gatgcgggcc
     3001 tctgtcatcg gaccggtgag gttgtgcaaa tcttccttct ttgcttcgat cagacggtcg
     3061 atgccgtaaa gagccatacg gcggtagtca ccgatgatgc gtccgcgtgc ataattgtcg
     3121 ggaagtccgg taaggaatcc cagcgaacgg aaggaacgga tttcttccgt atatacgtcg
     3181 aatactccgt cgttgtgcgt cttgcggtag tgagtgaaaa tatcttttac gcggtcgtcc
     3241 acttccacgc cgttttcgtg acaggctttg ctgactacgt tgatacctcc gaaaggcttg
     3301 atggcacgtt tcagaagttc gtctgtctgc aggccgacga tcagttcgtt ttccttgtcg
     3361 atatatccgg ccttgtggga agtgatggtt gatacggtaa cattatctaa tgagcggaca
     3421 ccgttgtttt ctctttcttc tgccagtgct tcgaggcagc ggttccatac ggctttggta
     3481 cgttctgtag gtccttcgag gaatgaagca tctccgtaat acggagtgat gttatgactt
     3541 acgaaatctc tgacgttgat ttcgctgctc caaagaccgt ctttaaagat cttgtttaat
     3601 tccataaaat gataaaatta gaggttgtag ttatcctttt ctttagagcg cacaaagtta
     3661 c
//
