LOCUS       c00142_gnlPro..         7404 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PNBLHELC_142.
ACCESSION   c00142_gnlPro..
VERSION     c00142_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 06:33:00
            Original ID  :: gnl|Prokka|PNBLHELC_142
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7404
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7404
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3842:6822]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3843..6822
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7404
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7404
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             311..3415
                     /locus_tag="ctg142_1"
                     /transl_table=11
                     /translation="MNKKFSTLLTMGLLATSSLCGSAWAQTAPTINGIELEKVAAPANN
                     AAWTGGPYVLVADEDGTLEGSDLLLQAVVSEDGKTVKYYGENLSAFATGAKLKEITWTF
                     TETPVKDVAGVPNGYYYTLTSAASGKPLTVNQSGDLIEKLENCKLDAANHLYTQFSVLS
                     NDFTSRFLQGSGLYPYAGGAGLNPVSLTRAELTSIVGLTGSKLYLYKLKTQELVDQVAV
                     EAMNETMAGEGFNFDFTPKKKLDWANDVLSDVKLKAFYVDNDFDLGNDMKIPAGVYFAT
                     SYPEELVGTDKIADSDLFKQCTFIAIDPTGNYDINATDRDNGLGYELITMQGSEMNFFH
                     GAATSDNFSHAEEVFVGNACFVVEVPNAVTAPETYNIKAKNIHVFVTATDNAAKKHVAK
                     AGLYIGCIADQNKNYMVTTVVTDALAFTTTNTTLLDGDGLVGFLQEEDAPSVYTIQFVS
                     GEKNEGASEYEQYLTVNKPAGFELASTVDFNEADPMYQFVVTGVDKTNKTITLANRQTK
                     VDFTVSLYENEDGVYTVLPATGSKDKVYVEYINGTVGNNSKDEVVTFEAIDLTNMKIVL
                     TPYTVEDKFATFVNRADNMGLVQFELALNSDIAPAFYVGGEKDVDGNLAPNKDILAYAD
                     ADSMVQFELIKSKKPVNVLNNYIYLDGKRIRTSTERDTVAFYTYAVKAFDDANDRYLSN
                     TWELEVATDVSADEFIIKQNLDGSVSLIKAATTTDVNGAFAMVNDLKKDRETAWKDAYN
                     YELEHKIDKGLKTFMVDETPAVSLEAVPQHVSFALTRGGFLTNKDNDAFQSIATEASED
                     LTFWVDTVKSDKNIPTFYISKAGHFLYNAEDSVEYYEARGNYRFNLENQKGEAAKLIFN
                     AGELVSSDTLRTVVDGKSVLVAEKDNAPKKIKGGLDNFQFQIIKAEDGSDEYVIRQGRA
                     NYVQMINSYFYLEANKNTAVRFLIESQSTPTANEGVAVSEVKVLAGEGNVTIAGAAGKK
                     VVISNILGQVVANTVISSDNAVIAAPAGVVVVAVEGEAAVKAIVK"
     CDS             3843..6083
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg142_2"
                     /sec_met_domain="Gly_radical (E-value: 3.2e-46, bitscore:
                     144.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.2e-282, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGETWKKEINVRDFIQNNYKPYNGDDTFLVPSSE
                     KTKKVWNKLTEMFKVEREKGVYDAETKLPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTDEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVPLYGTQILIEEKKRFQKRLDIQELTEEIIQSREEITEQIKAL
                     KAFEKMCAGYGFDVTRPAKDAREAVQFLYLAYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DICEGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPDNWKNFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDEKSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMGLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRKATPTQSILTITSNVVYGKKTGTTPDGRPGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLVDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINSSL"
     CDS             6100..6822
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73; E-value:
                     9.4e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg142_3"
                     /sec_met_domain="Radical_SAM (E-value: 1.4e-36, bitscore:
                     114.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGRIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWEVKR
                     ETPYLLEPEALLAEVLRYKNFIAKGGVTVTGGEPLLQPEFLKEFFRLCRENGIHTALDT
                     SGYICSGKALEVLEQVDLVLLDIKTIDAGLHPRLTAVKLDNTLRFLDELEKRGIPVWIR
                     HVIVPGLTDDDEALSKLAEYISSYNVVQKAELLPYHTMGAYKYEAQGLDYKLKGVEPLS
                     AERLANAKAIFMKHGITV"
     CDS             complement(6825..7130)
                     /locus_tag="ctg142_4"
                     /transl_table=11
                     /translation="MRRASLYNTFSASLLLVCSAWLSSCVSQIPLTKEKPVNNSTYQVE
                     YLFEHEGCKVYRFMDMGHYIYFTNCEGDVTSIESDSVRTVNRISRKPIIIKEQTLD"
ORIGIN
        1 agatcatgcc tagtctcgtg ggctcggaga tgtgtataag agacaggtat gtggctgata
       61 atctttgttt taatgaagat gtaaagaggt ttggcgaatg attgaaaaca gttactttta
      121 tatatggctt tagctacttt tgggtgctat aaattgttgt taataagaag tataggtgta
      181 tgagaagaaa aatggtgaaa aaacgtacgt tttatctcgt caaaatacag gaatttggta
      241 aaaacctgtt aaacaggaag taagaacgat attcaatata catattatta ttaattaaac
      301 attttagatt atgaacaaga agttttctac tcttttgaca atgggcttgt tggctacaag
      361 ttcgttgtgt ggtagtgctt gggcgcagac tgctcctaca attaatggga ttgaattgga
      421 gaaggttgct gctcctgcca ataatgctgc atggacgggc ggtccttatg tgctggttgc
      481 tgatgaggat ggtacgcttg aaggctctga tcttttgctg caagcagtag ttagtgagga
      541 tggtaaaact gtaaagtact atggtgaaaa tctttcagct ttcgcaactg gtgctaagtt
      601 gaaagagatt acttggactt ttactgaaac acccgtgaaa gatgttgctg gtgtacctaa
      661 tggctattat tacactttga ctagcgctgc ttctggtaag ccgttgacag taaatcaatc
      721 aggagatctt atagaaaaat tagaaaattg taaacttgat gcggctaatc atttgtatac
      781 tcaattctca gtgctgtcta atgattttac aagccgcttt ttgcaaggtt caggattgta
      841 tccctatgca ggaggggcag ggttgaatcc tgtttcattg acacgtgcag aacttacctc
      901 tattgtaggt ttaactggaa gcaagttgta tctctacaaa cttaaaaccc aggaattggt
      961 tgaccaagta gctgttgaag ccatgaacga gactatggct ggcgaaggtt ttaacttcga
     1021 tttcactcct aaaaagaaat tggattgggc aaacgatgtt ctgtctgatg tgaaattgaa
     1081 agcgttttat gttgataacg attttgacct aggtaacgac atgaagattc ccgcaggtgt
     1141 ttactttgct acttcttatc cggaagaatt ggtcggaaca gataagattg ctgattcgga
     1201 tctgttcaaa cagtgcacgt tcattgctat cgacccgaca ggcaactatg acatcaacgc
     1261 gactgaccgt gataatggct taggttacga actgataact atgcagggta gtgaaatgaa
     1321 ctttttccat ggtgctgcta catctgacaa cttctctcat gcagaagagg tattcgtagg
     1381 caatgcttgt ttcgtagttg aagttcctaa tgcagtgact gctccggaaa catacaatat
     1441 caaagccaag aatattcacg tgtttgttac tgcaacagac aatgcagcta aaaagcacgt
     1501 cgccaaggct ggtctttata ttggttgtat cgctgatcag aataagaact acatggtaac
     1561 tactgttgtt actgatgctt tggctttcac tacgactaat actactctgt tggatggtga
     1621 tggtttagtt ggcttcttgc aggaagaaga tgcaccatct gtttacacaa tccagtttgt
     1681 aagcggtgaa aagaatgaag gtgcttctga gtatgaacaa tatctgactg tgaataaacc
     1741 ggctggtttc gaattagctt ctactgttga tttcaacgaa gcagacccga tgtaccagtt
     1801 tgttgtaaca ggtgttgaca agacaaacaa gacaatcact ttggctaacc gtcaaacaaa
     1861 agttgatttc actgtttcgc tgtatgaaaa cgaagatggt gtatatacag tacttccggc
     1921 aacaggcagc aaggataagg tatatgtaga atatatcaat ggtacagttg gaaataattc
     1981 aaaggatgaa gtcgttactt ttgaagctat tgacttgacg aacatgaaga tcgtcctgac
     2041 tccgtacact gtagaagaca agttcgctac attcgtgaac agagctgaca acatgggctt
     2101 ggttcagttc gaattggccc ttaacagcga tatcgctccg gcattctatg ttggtggcga
     2161 gaaagacgta gatggcaatc tggcaccaaa caaagacatc ttggcttatg ccgatgcaga
     2221 cagcatggtt cagtttgaac tgattaagag caaaaagccg gttaatgtat tgaacaatta
     2281 catctacttg gatggcaaac gtattcggac ttcaacagaa agagacacag tcgctttcta
     2341 tacttatgcg gttaaggcgt ttgatgatgc aaacgacaga tatctgagca acacttggga
     2401 acttgaagta gcaacagatg tgtctgcaga tgaattcatc atcaagcaga atttggatgg
     2461 ttccgtatca ttgatcaaag ctgcgactac aactgatgtt aatggagctt tcgcaatggt
     2521 taatgactta aagaaagaca gagaaactgc ttggaaagat gcatacaact acgagttgga
     2581 gcataagatc gacaaaggcc tgaagacctt catggttgac gaaactccgg ctgtcagcct
     2641 agaagctgtt ccgcagcacg tatctttcgc actgactcgt ggcggtttct tgacgaacaa
     2701 ggacaacgat gcgttccaga gcatcgcgac ggaagcaagt gaagacttga cattctgggt
     2761 agacacagtt aaatctgaca agaatattcc tacgttctac atttctaagg ctggtcactt
     2821 cttatacaat gcagaagact ctgttgagta ttacgaagct cgtggcaact accgcttcaa
     2881 cttggaaaac cagaaaggtg aagctgctaa gctgatcttc aatgctggtg aattggtctc
     2941 ttccgacaca ttgcgcactg tcgttgacgg aaaatctgtc ctggtagcag aaaaagacaa
     3001 tgctccgaag aaaatcaaag gcggtttgga caacttccag ttccagatca tcaaggctga
     3061 agatggtagc gatgaatatg tgatccgtca gggtcgtgca aactatgttc agatgatcaa
     3121 tagctacttc tatctggaag caaacaagaa cacggctgtt cgcttcctga tcgaatccca
     3181 gtctactccg acagccaacg aaggcgtagc cgtatctgaa gtgaaagttc tcgccggcga
     3241 aggcaatgtg acaatcgcag gtgctgccgg caagaaggtt gtcatcagca acatcttggg
     3301 tcaggtagta gcaaacacag taatctcttc tgacaacgct gtaatcgctg ctccggcagg
     3361 cgtagtagtt gtagctgttg aaggcgaagc tgctgtaaaa gctatcgtta agtaacgatt
     3421 gatactaaaa aaacaatact gtctatcgta agggtggtta gtgaaccacc cttacacagg
     3481 cacaatgaca ttttataatc aaataattct agacaacctg cgtgaaaccg tatgtaagag
     3541 gttttcttga tctacggttt caattaccct gcgacaggcg aacgagcggt tggatttaat
     3601 attaataata ctaaagtaat gaaattaaaa gttctactgc ttttccctct gtgaagaccg
     3661 gacggcagac aaaaaacagg cctcggcggt attcgtactg ccggggcttc gtttttattt
     3721 cccttatccc tatttgtagg tattttctcc ggtttaacat ccctcgtcct ttacaatcct
     3781 tttcagatag ggggaaaaga cgtatctttg cgtgcctaaa ccgaaataga caagataagc
     3841 ttatgaagtt tgaaaacgag aactgggcgc agttcaaagg agaaacctgg aaaaaggaga
     3901 tcaatgtaag agactttatc cagaacaatt acaaacctta taacggagac gatacgttcc
     3961 tcgtaccctc ttcggagaaa acgaagaaag tgtggaacaa actaaccgag atgtttaagg
     4021 ttgaaaggga gaaaggagta tatgatgccg aaacgaagct tccccagggg attgacacat
     4081 acggacccgg ttacatcgat aaggaaaacg aagtaatcgt cggcttgcag acagatgccc
     4141 cgttgaaacg tggtatcttc ccgaaaggcg gtatccgcat ggtcgaaaac agtctggaag
     4201 cctatggtta tcatctggac ccgatgacaa aagagatctt caccaaatac agaaagacgc
     4261 acaacgaagg cgttttttca gcctatacag acgagatgat tgcggcacgt cgttccgcca
     4321 tcattaccgg gttgcctgat gcttacggac gcggacgtat catcggtgat taccgtcgtg
     4381 tacctctgta cggaacccag atcctgatcg aagaaaagaa acgtttccaa aaacgtctcg
     4441 atatacagga actgacagaa gaaatcattc agagccgtga ggagataacg gagcagatca
     4501 aagcgctgaa agcatttgag aaaatgtgtg ccggttatgg tttcgatgtg acacgtccgg
     4561 caaaggatgc ccgcgaagcc gtgcagttct tgtatctggc ttacctggca gccgtaaagg
     4621 atcaggatgg agcagccatg tctctcggac gtacttctac tttcctcgat atctatatcg
     4681 aaaaagatat ttgcgaaggc aagctgacgg aagaagaggc acaggaattg atcgaccagt
     4741 ttattatcaa actgcgtatt gtccgtttcc tgcgtactcc ggaatacaac gacctgttct
     4801 ccggcgaccc tgtttgggtg actgaatcgc tgggcggaca aggtgttgac ggccgttcgc
     4861 tggtgacaaa gacatcttac cgttatctgc ataccttgta taacctcggc ccggcacccg
     4921 aacccaatct gacggtgctt tggttcaaca atgctccgga taactggaaa aacttctgcg
     4981 caaaagtatc gatcgatacc tctgccatcc agtacgagaa cgacgatctg atgcgtccgg
     5041 actatggcga cgattacggt atcgcctgct gtgtttctcc aatgaagatc ggtaagcaga
     5101 tgcagtattt cggagctcgt gccaacctgg caaagtgctt gttgtatgcg atcaatggcg
     5161 gacgtgacga gaaatcgggt gtgcaggtgg ctccccgttt cgaaccgatc accagcgagt
     5221 atctggacta caacgaagtg atggagaaat acgagcagat gatgcgctgg ttggcaaaag
     5281 tctatgtcaa tgcactgaag atcattcact acatgcacga caaatatgcg tatgaagcat
     5341 tcgagatggg gcttcatgac ggagatgtgg aacgtatccg cgcaaccggt atcgccggcc
     5401 tttctatcgt tgccgactcg ctggctgcca tccgtgacac gaaagtgaaa gtgatccgtg
     5461 acgaacgtgg tttggctgtc gacttcgaac gcgaaggcga atatgtacct ttcggaaaca
     5521 atgacgaccg cacggacagt attgctgtcg acattactga gaagtttatg gaatatctgc
     5581 gccagcatca gacataccgc aaggcaactc cgacacaatc tatcctgacg atcacatcaa
     5641 acgtggtcta cggaaagaaa accggaacga ctcccgacgg ccgtccgggc ggtacgccgt
     5701 ttgctccggg agccaacccg atgaacggac gcgatacgaa aggggctgtc gccgctttgg
     5761 cttctgtcgc caaactgcct tttcagcatg cgcatgacgg tatttcctat acattcgccg
     5821 tttcaccggc tacgttgggt aaggagcgcg atattcagat aaataacctg gtgagcctgt
     5881 tggatggcta tttcacgcct gacggcggcc agcatctgaa tgtgaacgtg ttcgataagg
     5941 acctgttggt cgatgcgatg gagcacccgg agaaataccc acagcttacg atccgtgttt
     6001 cgggctatgc cgtcaacttc gtgaagctga cacgcgaaca gcagcttgac gtcatctcgc
     6061 gcaccatcaa cagcagttta taattgtcca ttgattaata tgttggggcg tatacattct
     6121 ttagagagtt tcgggaccgt tgacggtccc ggaattcgct ttgttgtttt catgcaggga
     6181 tgtccgcttc gttgtctgta ttgccacaat ccggatacct gggaggtaaa gcgtgaaacg
     6241 ccttatctgc tggaacctga agcgttgctt gccgaagtgc tgcgctataa gaattttatc
     6301 gctaaaggtg gtgttaccgt gaccggtggc gagcctttgt tacaacctga attccttaaa
     6361 gagtttttcc gcctttgccg tgagaacggt attcatacgg cgttggacac ttccggctat
     6421 atctgttccg gtaaagcatt ggaggtgctc gaacaggtcg atctggttct gttagatatc
     6481 aagacgatcg acgccggtct tcatccccgt ttgacggccg taaaactcga taatacttta
     6541 cgctttcttg atgaactgga gaaacgcggc atccctgtat ggattcgcca cgtgatcgtg
     6601 cccggcctga cggacgatga tgaagccttg agcaagctgg ccgaatatat cagttcatat
     6661 aacgtggtcc agaaagccga acttctgcct tatcatacaa tgggagctta caaatatgag
     6721 gcacaaggcc tggactataa gctgaaaggt gtggaaccgc tttcagcaga gcgtctggcc
     6781 aatgcgaaag cgatctttat gaaacacggg ataaccgtat aagcctaatc gagtgtctgc
     6841 tctttgatga tgatcggttt acggctgatc cggttgactg tgcggacact gtcgctttca
     6901 atactggtaa catctccctc gcagttggtg aaatagatgt aatgtcccat atccatgaaa
     6961 cgatagacct tacacccttc gtgttcgaac agatattcga cttgataagt ggaattgttt
     7021 accggttttt cctttgtaag gggtatttgt gagacacatg aagaaagcca tgcgctgcaa
     7081 acgagcaata gacttgctga aaatgtatta tacaaactag ctcttctcat cttcgaatat
     7141 ttttaagtta acatccaaag gtaacaatta aagaatcaca tggtatgacc gaaaggtatt
     7201 ttacctacaa ttaggtatat ttttattgtt ccggaaaaca ttactttgct tcctattgtc
     7261 atagtagata agtatgcttg cagatagaag tccgtctatc ggcagataaa aattatcaat
     7321 ttgaaaaatt ctttcaatca gtatcttacg atttaccctg tctcttatac acatctccga
     7381 gcccacgaga ctaggcatga tctc
//
