LOCUS       c00194_gnlPro..         6042 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PNBLHELC_194.
ACCESSION   c00194_gnlPro..
VERSION     c00194_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 06:33:00
            Original ID  :: gnl|Prokka|PNBLHELC_194
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6042
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6042
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[120:2848]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      121..2848
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..6042
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6042
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             121..2064
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 196.1; E-value:
                     3.7e-60)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg194_1"
                     /sec_met_domain="FAD_binding_2 (E-value: 2.4e-61, bitscore:
                     196.1, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 1.7e-40,
                     bitscore: 126.2, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQIDSKIPEGQLAEKWSNYKAHQKLVNPANKRRLDIIVVGTGLA
                     GASAAASLGEMGFRVFNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKG
                     GDYRAREANVYRLAEVSNAIIDQCVAQGVPFARDYGGTLDNRSFGGAQVSRTFYARGQT
                     GQQLLLGAYSALSRQVQKGTVKLFTRYEMLDLVIIEGRARGIIARNLVTGEIERFAAHA
                     VVIGTGGYGNAFFLSTNAMGSNGSVAIQCYKKGAYFANPCFAQIHPTCIPVHGDKQSKL
                     TLMSESLRNDGRIWVPKKIEDAKALQAGTKKPNDIPDEDRDFYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNNTGLAVFLDFKYAIDRLGEDVVRARYGNLFDMYEEITDENPYK
                     TPMMIFPAIHYTMGGIWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIQNYLADQIQVPRFSTDLPEFVAAEKAVKDKIQKIKAVNGKHSVDSIHKKLGHI
                     MWDFVGMARTKESLQKALSGIQEVKKDFWTNVRIPGEVNELNVELEKALRLIDFIEVGM
                     LMARDALNREESCGGHFRTEYQTPEGEALRDDKNFSYVSCWKYTGEDSEPELIKEDLNY
                     QFVKVQTRNYKS"
     CDS             2093..2848
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer4_7"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg194_2"
                     /sec_met_domain="Fer4_7 (E-value: 3.8e-13, bitscore: 38.1,
                     seeds: 208, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_3 (E-value: 5.9e-27, bitscore: 82.1,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.4e-07, bitscore: 20.0,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKNISFTLKIWRQKSPKAKGAFETYQMKDIPGDTSFLEMLDILN
                     EQIINDGGEPIVFDHDCREGICGMCSLYINGHPHGPATGATTCQMYMRRFKDGDTITVE
                     PWRSAGFPVIRDLMVDRSAYDKIMQAGGYVSVNTGAPQDANAILISKDIADEAMDAAAC
                     IGCGACVAACKNGSAMLFVSAKVSQLNLLPQGKVEAARRAKAMLSKMDELGFGNCTNTR
                     ACEAECPKNISISNIARLNRDFILAKLKD"
     CDS             complement(2945..3640)
                     /locus_tag="ctg194_3"
                     /transl_table=11
                     /translation="MITPSAVAGPFDIAIWWCGAKSASNTEKLTVSVKTVDAEEWTELG
                     TLSNDAYKNIRKQVISYEGTEPVLVKFASASENGKNNNAMIFDIKLLGEGEDPVLPVAT
                     PVITTEDTADGKMVTIACETEDAAVYYTLDGTEPTVESSLYAEPFETKSSCTVKAIAVK
                     EGFPDSEIAELDITIATSIISETASKVILSRSYFTVGGVKLEVPVKGLNIVRTVFEDGV
                     IEMSKVIVD"
     CDS             complement(3845..6040)
                     /locus_tag="ctg194_4"
                     /transl_table=11
                     /translation="ASDVYKRQRYKNLETVDAKNVLKANGVVIAETDGILFPALTKGNL
                     SLRINMGDDGIQLGSKNLEVTIKELKAGQHVTIILKSANATSGRGISAITNMTGTVGSD
                     TYKTGNDGENTYELVVAADGDVKFKYNEGVILKSIIVSEETVARKVAYIYDSSYAGYVL
                     DEDPVHAALIDAYEVTDIDVKSLDGSTVNELLGYDLVFSSEAVGGKHAYGLKLETVVNK
                     VPMLNLKSFYYSSDRWAWATGQNPDKGTNTMTILEAYRNHAIFNGVAIAEDGTCVIFTE
                     SDASNNQVQAYKDPGAIIADDIVMAKDGTGTYNTIHEHGTTNKYMLIPFASNLIRGIDA
                     NAIKLLLNAADYLIATKEVYVEPSDPVAAPVISDVVNGNAKTVTITTATEDAAIYYTLD
                     GTVPTIESILYTQPFDVLKPCSVTAFAVKEGMLDSEVVSKDIENENYIARNKTLLWANF
                     KDQPEEWGVTGDIIGSGKTDEKTIAGFIIGSKGQRVNLQATGVSEKIGDTYGPATEEDM
                     GATSYAMSFLTGSASGYIITPSVVAGPFDVAIWWCTAKSASYTEKLTISVKTADAEEWT
                     ELETISNKSYKSVRKQIVSYDGTEPVQVKITCASGNGSNNNAMIFDVKLLGEGADPVAT
                     PVISAEANGNAKTVTITTDTKDATIYYTINGTDPTIASTQYTEPFDILKPCTVKAFAVK
                     KDMADSDIASEDIENENYIARNKTLLWANFKNQPEEW"
ORIGIN
        1 ctattcaaca atcgttgtca tctgctttgc actggtagta gtcgtattct acgttcaatc
       61 gttatgcggc gcttcttgct aaattttaaa attgtaaatg attaaatcgt aaatagcatt
      121 atgactcaaa tagattctaa aattcctgaa ggccagttag ccgaaaaatg gagtaactat
      181 aaagctcacc aaaaacttgt taatcctgcc aacaaacgtc gtctcgacat tatcgttgta
      241 ggaaccggac ttgccggcgc atctgcagcc gcttctctgg gtgaaatggg attcagagta
      301 ttcaacttct gtatccagga ttctccacgc cgtgctcact ccatcgcagc acaaggtggt
      361 atcaacgcag ctaagaatta tcaaaatgac ggcgactctg tataccgtct tttctacgat
      421 acaatcaaag gtggtgacta tcgtgcacgc gaagccaacg tttaccgtct ggcagaagta
      481 tccaatgcca tcatcgacca atgtgtagct cagggtgttc cttttgcccg tgactacggt
      541 ggtacgctcg acaaccgttc tttcggtggt gcgcaggtat cacgtacatt ctatgcccgt
      601 ggccagaccg gacagcagtt gttgctgggc gcttattcag cattgagccg tcaggtacag
      661 aaaggtactg taaaattgtt cacccgttat gaaatgctcg accttgttat aatcgaagga
      721 cgcgcccgtg gtatcattgc ccgcaacctc gtaaccgggg aaattgaacg tttcgctgct
      781 catgccgtag ttatcggtac aggcggttac ggaaatgctt tcttcctttc aaccaatgca
      841 atgggctcca acggttctgt tgccatccag tgctacaaaa agggtgctta ttttgctaat
      901 ccttgttttg cacagatcca cccgacctgt atccctgttc acggcgacaa acagtctaag
      961 ctgacgctga tgtctgaatc tctccgtaac gacggtcgta tctgggttcc gaaaaagatc
     1021 gaagatgcca aagcattgca ggcaggaact aaaaaaccga atgatatacc cgacgaagat
     1081 cgtgacttct atctggaacg tcgttatccg gcattcggta acctcgtacc tcgcgacgtg
     1141 gcatcacgtg ctgccaaaga acgttgcgat gcaggtttcg gtgtaaacaa taccggcttg
     1201 gccgttttcc ttgatttcaa atatgccatc gatcgtctgg gtgaggatgt ggttcgtgcc
     1261 cgttatggta acctcttcga tatgtacgaa gaaatcactg acgagaatcc atataaaact
     1321 ccgatgatga tattccccgc tatccactac acaatgggtg gtatttgggt agattatgaa
     1381 ttaatgactt ctattccggg tctgttcgct atcggcgaag ccaacttctc cgatcacggc
     1441 gccaaccgtt tgggtgcatc cgcgttgatg cagggtctgg ccgacggata tttcgtattg
     1501 ccatacacta tccaaaacta tctggccgat caaatccagg ttccgcgttt ctctaccgac
     1561 cttccggaat tcgtagcagc cgaaaaagct gtcaaggata agattcagaa aatcaaagcc
     1621 gttaacggta aacactctgt tgactccatc cacaagaaat tgggtcacat catgtgggat
     1681 tttgtaggta tggctcgtac caaagaatct ttgcagaaag ctttgagcgg tatccaagaa
     1741 gtgaaaaaag acttctggac caacgtacgc atcccgggtg aagtaaacga actgaacgta
     1801 gaacttgaaa aggctctccg tctgatcgac tttatcgaag taggtatgtt gatggcccgc
     1861 gatgctctga accgtgaaga atcttgcggc ggtcacttcc gtactgagta tcagactccg
     1921 gaaggcgaag ctttgcgtga cgataagaac ttctcttacg tatcttgctg gaaatatacc
     1981 ggtgaagatt cagaaccgga attgatcaaa gaagatttga actatcagtt cgtgaaagtt
     2041 cagactcgta actacaaatc ttaatcggct aatcaattaa aggaaaacaa aaatggataa
     2101 aaatataagt ttcacactga agatatggcg tcagaaaagc ccgaaagcaa aaggtgcttt
     2161 tgaaacatat caaatgaaag atatcccggg cgatacttcg ttcctcgaaa tgctggatat
     2221 cctgaatgaa caaatcatta acgatggcgg tgaaccgatc gtattcgacc atgactgccg
     2281 cgaaggtatc tgtggtatgt gctctttgta catcaacgga catccgcatg gtcctgcaac
     2341 aggtgccact acctgccaga tgtatatgcg ccgtttcaaa gatggcgata caatcactgt
     2401 agaaccttgg cgttccgccg gcttcccggt aatccgcgac cttatggtag accgttctgc
     2461 ttatgacaaa atcatgcaag caggtggtta tgtaagcgta aataccggcg ctccacaaga
     2521 cgctaacgct atcttgattt ccaaagacat tgctgacgaa gctatggatg ctgctgcatg
     2581 tatcggttgc ggtgcttgtg tagctgcatg taagaacggt tcggccatgt tgttcgtttc
     2641 ggcaaaggtc agccagttaa acctgcttcc gcaaggaaag gtagaagctg cccgccgcgc
     2701 aaaagccatg ttgtctaaga tggacgaact cggattcggt aactgtacaa atacccgtgc
     2761 atgtgaagct gaatgtccga agaatatttc tatcagcaac atcgctcgtt tgaaccgcga
     2821 tttcatcctc gcaaaactga aagactaagc gtataactta gcatttgatt aataacagaa
     2881 aatccctgcc gggtcatccg gcagggattt ttatatatac ctattgaata agagaaattg
     2941 tgaactagtc cactattacc ttggacatct cgatcactcc gtcttcaaac accgttctta
     3001 cgatattcaa tcctttcacc ggaacttcaa gcttcactcc gccaacagtg aaataagagc
     3061 gggataagat aactttagaa gctgtttcag aaataatgga agtagcgata gtaatatcca
     3121 gttcggcaat ttccgaatcg gggaaaccct ctttcactgc gatagctttc actgtgcaag
     3181 atgatttcgt ctcaaacggt tctgcataaa gactactttc tactgtaggt tcagtgccat
     3241 ccaaagtgta gtagacagcc gcatcttctg tttcacaagc aatcgtgacc attttcccat
     3301 cggcagtatc ttctgtcgta atgaccggag tagccaccgg cagtaccgga tcttcacctt
     3361 cacctaataa tttaatatcg aatatcatcg cattattgtt ctttccgttt tccgaagcag
     3421 aagcaaactt aaccaatacc ggttctgttc cttcataaga aatcacttgt ttacggatat
     3481 tcttataagc atcattagaa agtgttccca gttctgtcca ttcttcagca tccaccgttt
     3541 tcaccgatac ggtcaacttc tccgtattgg aagctgattt tgcaccacac caccagatag
     3601 caatatcaaa cggtccggct acggcagaag gagtaatcat ataagcgctg gccgttcctg
     3661 tcaagaatga catggcataa cccgacgcac cggcatcagc ttctgtctcc gggccataat
     3721 ttccgcctat ttcttcagaa acaccggtag tctggaggtt tacacgctgg ccttcacttc
     3781 cgattataaa tcctgcaacc gttttctcat ccgtctttcc ggaagcgatt atatccccgg
     3841 tgagtcacca ctcttccggt tggttcttga agttggccca caacagggtt ttattccgtg
     3901 cgatataatt ttcattctca atatcttccg aagctatatc cgaatctgcc atatctttct
     3961 tcacggcaaa tgcttttact gtacaaggct tcaagatgtc aaaaggttct gtatattgcg
     4021 tgctggcaat cgtaggatct gttccattaa tcgtataata gatagtagca tctttcgtat
     4081 ccgtagtgat ggttactgtc ttggcatttc catttgcttc tgccgaaata accggagtag
     4141 ctaccggatc tgccccttcg cccaacaact taacatcgaa tatcattgca ttgttgttag
     4201 agccgtttcc ggaagcacaa gttatcttaa cttgtacagg ctctgttcca tcataagata
     4261 caatttgctt acgaacactc ttatagcttt tattagagat cgtttcaagt tctgtccact
     4321 cctctgcatc cgcagttttc acagatatag tcaatttctc tgtgtaagat gcactctttg
     4381 ccgtacacca ccaaatagca acatcaaaag gtccggctac tacagacgga gtaattatat
     4441 agccactggc agagcctgta aggaaagaca ttgcatagct tgtcgctccc atatcttctt
     4501 ctgtagcggg accgtaagta tcaccgatct tttcggacac acctgtagcc tgcagattaa
     4561 cacgctgccc cttgcttccg attataaatc ctgcgattgt cttttcatcc gtctttccgg
     4621 aaccgattat atccccggtt actccccatt cttccggctg atccttgaaa ttggcccata
     4681 acaacgtttt gtttcgtgca atataatttt cgttctcaat atccttcgac actacttctg
     4741 aatcgagcat tccttctttc acagcaaaag ccgttaccga acacggcttc aaaacatcaa
     4801 atggttgcgt gtataggata ctttcaatag taggaacagt accgtccaaa gtatagtaaa
     4861 tcgcggcatc ttctgttgca gtagtaatag tcactgtttt agcgtttcca ttaacaacat
     4921 ccgagataac cggagcagct accggatccg acggctcaac atatacttcc ttcgtggcaa
     4981 tcagataatc ggccgcattc agcaacagtt taattgcatt ggcatcaatc cccctgatca
     5041 gattactggc aaacggtatc aacatatact tatttgtcgt accatgttca tgaatggtat
     5101 tataagtgcc tgttccatct tttgccataa caatgtcatc agcaatgatt gcacccggat
     5161 ctttataagc ttgcacttga ttgttgctgg catcgctctc ggtaaaaata acacatgtac
     5221 cgtcttctgc gatggccact ccattgaaaa tagcatgatt acgataagct tccaatatag
     5281 tcatcgtatt agttccttta tccggattct gccccgtagc ccatgcccaa cggtccgacg
     5341 aataatagaa tgacttcaga ttgagcatag gcactttatt tacaacggtc tccaacttca
     5401 acccatacgc atgtttaccg ccaacagcct cagaagaaaa taccagatca taaccaagta
     5461 attcatttac cgtagaacca tctaaagatt tcacatcgat atccgttacc tcataggcgt
     5521 caatcaatgc agcatgtaca gggtcttcat cgagtacata accggcataa gaagaatcat
     5581 aaatataagc tactttacgt gcaaccgttt cttcggatac tataatactt ttcaagataa
     5641 caccttcatt gtatttgaac ttcacatcac catcagcagc gactactaac tcataagtat
     5701 tctcaccatc attgccggtc ttataagtgt cagatcccac cgtacctgtc atgttcgtta
     5761 tggcgctgat accgcgtcca cttgtcgcat tcgcactttt cagaataatg gtaacatgct
     5821 gtccagcttt cagctctttg attgtcactt ccagattctt acttcctaac tggataccat
     5881 catcacccat atttatacga agactcaaat ttcctttggt cagtgcagga aagagaatgc
     5941 catcggtctc tgcaatcact accccattag ctttcaggac atttttggca tccaccgtct
     6001 ctaaattctt ataacgctgt ctcttataca catctgacgc tg
//
