LOCUS       c00318_gnlPro..         6458 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HJPLAJIA_318.
ACCESSION   c00318_gnlPro..
VERSION     c00318_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 05:01:52
            Original ID  :: gnl|Prokka|HJPLAJIA_318
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6458
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6458
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:2546]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..2546
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6458
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6458
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..1802
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg318_1"
                     /sec_met_domain="Gly_radical (E-value: 2.2e-47, bitscore:
                     149.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 4.7e-287, bitscore:
                     941.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VYTEEIRSFRSLGFLTGLPDNYARGRIIGDYRRMALYGIDRLIEA
                     KKEDLRNLTGPMTEARIRLREEVAEQIKALKDMKVMGEYYGLDLSRPAYTAQEAVQWVY
                     MAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSKGTITESFAQELIDQFVIKLRMVRHL
                     RMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKTSFRFLQTLYNLGPSPEPNLTVLWSS
                     ELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQSDDYGIACCVSYQEIGKQIQFFGARC
                     NLAKALLLAINGGRCENTGTVMVKNIPVLTSDTLNFEEVMSNYKKVLTEIARVYNEAMN
                     IIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTARRNDIGLT
                     EGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVMYG
                     KKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISNTFSIVPK
                     SLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLNREMLYDAMEHPEKYPQLTIRVSGYA
                     VNFVKLSREHQLEVISRSFHERM"
     CDS             1818..2546
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg318_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.6e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(2618..3064)
                     /locus_tag="ctg318_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDRDFTAKDVDTAHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDENGKAADTRTSRQKEAMHRLVSELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIEEFES"
     CDS             complement(3051..3218)
                     /locus_tag="ctg318_4"
                     /transl_table=11
                     /translation="MKEIVTKILDVIMFLVPFFGKRKRNRIVREVRFNATHKEVCNVKT
                     TEREKDDEKD"
     CDS             complement(3239..3742)
                     /locus_tag="ctg318_5"
                     /transl_table=11
                     /translation="MAMTVTYSVVPRKNPAKKDESAKYYAQAQASGELDFEELCEGITS
                     RSTCTETDVRAAISGILYEAKRALKAGRIVRLGDLGSLQIGLNSEGAVSVKEFSSSLIT
                     AAHIIFRPGKTLADITKILSYQQVTTRAVAQTGGSGNEGEDDDKGSGGGSDGGGSGEAP
                     DPAA"
     CDS             3936..4166
                     /locus_tag="ctg318_6"
                     /transl_table=11
                     /translation="MYYPDQPFHIRTYKKSELAHLYNPNVCLKVALQILRRWIVYNLPL
                     LQELEQEGYRARNRLLSPRQVATIIRYLGEP"
     CDS             4254..6371
                     /locus_tag="ctg318_7"
                     /transl_table=11
                     /translation="MEHFTIFQDFYRVIAEMTEEEIVSAISNGTYRETVEDVRRIFAEQ
                     GEKAANEKKIELPEITFSANYRGGRSNATLVKYLGYIVVDIDHQTQEALARILALAKKC
                     AHTRIAFISPKGMGLKIVVRVCRTDGTLPETIQEIEDFHHAAYHKIASFYAQLCGVEVD
                     TSGQDVSRTCLFSYDPDIYFNPDATAVIVEQPPMFFKSQKKKRASGKKKKTTAPDNNPL
                     TEQVALNYHSSHASLMVTLNYYHNKSEKYVTGNRNNYLHCLACMYNRYGVPQEEAAAFI
                     KSQFTDLPADEMDALIGSAYGHNEEFDTRKLNSTQKRILQIEQHIKENYDTRYNEVLHI
                     MEYRRRKTDTEQPEPFHILDEMMENSIWMEMNELGYSCTVKTIQNLIYSDFSSTYHPIR
                     EYLDSLPEWDGTDYIGILANSVHTSHQKFWVECLERYLVGMCAAATQDDVVNHTVLLLC
                     SEIQNIGKTTFINNLLPPELRAYLSTGLINPNNKDDLAKIAQAMLINLDEFEGMNGREL
                     NIFKDLVTRKVISIRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHVDSME
                     FIKINYAQLYAQIKYLLNKPGYQYWFTQSENSRIEENNEDFIFHSPEEELVLTHIRKPE
                     RFEKVHYLTVTEIAELIRERTGYQYSHGTKAQLGKVMSKHGFEFHKGKNGRRYTVFIID
                     TEQVKSNRLYE"
ORIGIN
        1 acgtatatac cgaagaaatc cgttcgttcc gttctctggg attcctcacc ggacttcctg
       61 acaactatgc ccgcggacgt atcatcggcg actaccgccg tatggctctc tatggtatcg
      121 accgactgat tgaggcaaaa aaagaagatt tgcgtaacct taccggtccg atgaccgaag
      181 cccgcatccg cctgcgcgaa gaagtggcag agcaaatcaa ggcattgaag gacatgaagg
      241 taatgggcga atactacgga ctcgacttga gccgtcctgc ctacaccgca caagaagcag
      301 tacaatgggt gtacatggct taccttgctg ccgtaaaaga acaagacggt gctgccatgt
      361 cactaggtaa cgtctcttcg ttcctcgaca tttatatgga atatgaattg agcaaaggaa
      421 ccatcacaga atctttcgca caggagctga tcgaccagtt tgtcatcaaa ctgcgtatgg
      481 tgcgccacct gcgtatgcaa tcttacaacg acatctttgc cggcgacccg acatgggtaa
      541 ccgaatctct gggcggacgc ctcaacgacg gacgcaccaa ggtgacaaag acttctttcc
      601 gcttcctgca aacactctac aacctcggcc cttcaccgga accgaacctg actgtattgt
      661 ggagctcgga actgccggaa ggattcaaag aattctgcgc aaaagtatct atcgacactt
      721 cttccatcca atatgaaaac gacgacctga tgcgtgaagt acgccagtct gacgactacg
      781 gaatcgcgtg ctgcgtatct tatcaggaaa tcggaaaaca aatccagttc ttcggcgcac
      841 gttgcaacct ggctaaagcc ctgctgcttg ccatcaacgg cggacgttgc gaaaacaccg
      901 gcacggtaat ggtgaaaaac atccccgtac taaccagcga cacactgaac ttcgaagaag
      961 taatgagcaa ctacaagaag gtattgacag aaatcgcccg tgtttacaac gaagcgatga
     1021 acatcatcca ttatatgcat gataagtatt actacgaaaa agcccaaatg gctcttgtag
     1081 ataccaaccc gcgcatcaac cttgcttacg gtgtagccgg actttctatc gccctcgatt
     1141 cactgtcggc catcaaatat gccaaagtga ctgcacgccg caatgacatc ggtctgacag
     1201 aaggtttcga tattcaaggc gaattccctt gcttcggtaa tgacaacgac aaggtagacc
     1261 acctgggtgt tgacctggtc tatttcttca gcgaagaatt gaagaaactt ccggtttaca
     1321 aaaatgcccg tcctactctg tctttgctca ccatcacttc caacgtgatg tatggcaaga
     1381 aaacaggtgc tactcccgac ggacgcgcca aaggtgtcgc ttttgctccg ggagccaacc
     1441 cgatgcacgg acgtgacaag aacggtgcta tcgcctcttt gagttccgta gcgaaattgc
     1501 gttaccgcga ctcacaagac ggtatcagca acaccttctc catcgttccg aaatcactgg
     1561 gagctactga agaggatcgc gtagaaaatc tggtgacaat gatggatggt tacttcacca
     1621 aaggcgctca ccacttgaac gtaaatgtgc tgaaccgtga gatgctttac gacgccatgg
     1681 agcatccgga aaaatatccg cagcttacca tccgtgtttc cggttatgcc gtaaacttcg
     1741 tgaagttgag ccgcgaacat caattggaag taatcagccg tagcttccac gaacgtatgt
     1801 aatcagatct actcaatatg acgataaacg tacattcata cgaaagtatg ggaacattcg
     1861 acgggccggg cttacggctc gtcgtttttc ttcaaggatg caatttccgc tgcctgtatt
     1921 gtgccaatcc cgacacgata gccggaaaag gaggtacacc taccccaccg gaagaaatcg
     1981 tccgcatggc tatgagccaa cgtccttttt tcgggaaacg cggaggagtc actttctccg
     2041 gaggagaacc tacgtttcag gccaaagcac tggttcccct ggtgcgtgaa ctgaaagaaa
     2101 agggaataca tgtatgcata gacagcaacg gcggtatctg gaacgaagaa gtagaagaac
     2161 ttttcaaact gacggatctt gtattgctgg atattaaaga gttcaatccg gcacgccatc
     2221 aggcactgac cggaagaagt aacgagcaaa ccatccgcac ggcagcatgg ctggaagaaa
     2281 acgagaaacc tttctggctg cgttatgtat tagtgcccgg atacagcgat ttcgaagaag
     2341 acatccgccg tttaggagag gctctgggaa aatataagat ggtgcaacga gtggaaatcc
     2401 tgccttatca tacattaggc gtacataagt atgaagcgat ggagcaggaa tacaaactga
     2461 aagacgtaaa agaaaatacc cccgaacaac ttgaaaaagc cgcggaggta tttaaagagt
     2521 atttcactac tgtggtggtc aattagttga agaagttgag aattgagagt taactatgca
     2581 gtataacagt gcgcagccaa ctctcaactc tccattctca actctcaaat tcttcaatca
     2641 cctcaaaaca gggacattct ttgatccgtt cccaggggtc tacaattccg ttgtagttgg
     2701 tgtcagggct caagtcccgg tgaccgacca cctttgcatc cggataacgc tgcaacaact
     2761 cgcttaccag acgatgcatc gcctctttct ggcgggaagt acgtgtatcc gctgcctttc
     2821 cgttctcatc cagcccgcct tcatagcaca cccctaaact aatcgcatta aaaccgcgtg
     2881 catgagcacc caccgtatct tcatccctca tcggttctat ctgtcccgac ttgcggatat
     2941 aataatgata cccccaacac gaaaagcccc gaaagcgatg ggcagtgtcc acatccttcg
     3001 ccgtgaagtc gcgatcgaca cgggtagcag agcagtgaat cacaatcaga ctaatctttc
     3061 tcatcgtcct tctccctttc cgtcgttttc acattacaca cttccttatg ggtagcattg
     3121 aagcgcacct ctctcacaat tctgttccgc tttctttttc cgaagaaagg cacaaggaac
     3181 atgattacat ccaaaatctt agttacaatt tctttcattt gatctaatat taaagttatc
     3241 atgctgccgg atccggagct tctccgcttc caccaccgtc tgaaccgcca ccggaacctt
     3301 tgtcatcgtc ttctccttca ttgccgctac cgcccgtttg ggccaccgca cgcgtcgtta
     3361 cttgctggta gctaagaatc ttcgtgatgt ccgccaacgt ctttccggga cggaaaataa
     3421 tatgggcagc cgtaatcagc gaacttgaaa actccttgac cgacacagct ccttcactgt
     3481 tcaacccgat ctgcaaactg cccaaatcac ccagccggac tattcttccc gctttcaacg
     3541 cacgcttcgc ctcataaaga atacccgaaa tggcggcacg cacatctgtc tccgtacagg
     3601 tagaccgact ggtgattccc tcgcacaact cttcaaaatc caactctccc gaagcctgtg
     3661 cctgcgcgta atactttgcc gactcatcct ttttggcagg attcttgcgc ggcactactg
     3721 aataagttac tgtcattgcc ataataaaat aattttaaat tttcatacat ttcgagcgcg
     3781 ttccttattt gttaacgaca taaagataac acatttttca ccctccggat tccccaacga
     3841 agtttcctga tattctatga ataataatga cgttattctt ttaataaacc attaattttc
     3901 agtaacttta tagcataaac caaacatcat tcatcatgta ttatccggat cagccgtttc
     3961 atatccgcac ttataaaaag tcggaactag ctcatcttta caaccccaac gtatgcctca
     4021 aagtagcctt acaaatacta cgtcgttgga ttgtctacaa ccttccgctc ctgcaagaac
     4081 tcgaacaaga aggctatcgt gcccgcaacc gccttctctc tccccggcaa gtggcaacga
     4141 tcatacgcta tttgggagaa ccctaggaac ttaatcaaaa aaacgaaaag ttccattcta
     4201 ttattttaca aacaaatttc tttgcaaccg caaattaaaa agaacaaacc aacatggagc
     4261 attttaccat ctttcaagac ttttaccgcg tcatagccga aatgacagaa gaagaaatcg
     4321 tatcggccat cagcaacggc acataccggg aaacagtgga ggacgtgcgc cgaatcttcg
     4381 cggaacaagg cgagaaagca gcaaacgaaa agaaaataga actaccggaa atcacctttt
     4441 cagccaacta ccggggagga cgttcaaacg caactctcgt caagtatctg ggatacatcg
     4501 tggtagacat cgaccatcag acacaggaag cattggcacg catcctcgca ctggcaaaaa
     4561 agtgtgccca cacccgcatc gctttcatca gtcccaaagg aatgggactg aaaatcgtgg
     4621 tacgtgtatg ccggacggac gggacattgc ccgaaacgat tcaggaaatt gaagatttcc
     4681 atcatgccgc ttatcacaaa atcgcttcct tttatgccca gctctgcgga gttgaggtcg
     4741 acacttccgg acaggatgtc agccgcactt gcctgttctc ttacgatccg gatatttatt
     4801 tcaatcccga tgccacagct gtcatcgtgg aacagccacc gatgttcttc aagtctcaaa
     4861 agaagaaaag ggcatccggc aaaaagaaaa agacgacagc accagacaac aatcccctca
     4921 ccgaacaagt ggcactcaac taccattcct cgcacgcttc cttaatggtg actctgaact
     4981 attatcacaa taaatccgaa aagtacgtca ccggcaaccg gaacaactac ctgcattgcc
     5041 tggcttgtat gtacaatcgc tacggtgtcc cccaggaaga ggcggcagct tttatcaaga
     5101 gccagttcac cgatcttccc gcggatgaaa tggacgcact gatcgggagt gcttacggac
     5161 acaatgagga attcgacacc cgcaagctga atagtacgca gaagcgaata ttacagattg
     5221 agcaacatat caaagaaaat tacgatacac gctacaacga agtgcttcat atcatggaat
     5281 atcgcagaag gaaaacggac acggagcaac cggaaccttt ccatattctc gacgaaatga
     5341 tggaaaacag catttggatg gagatgaacg aactaggcta ttcgtgcacc gtcaaaacaa
     5401 ttcaaaatct gatctactcc gacttcagca gcacttacca tcctattcgc gaatatttag
     5461 actcgctgcc tgaatgggac ggaacggatt atatcggtat ccttgccaac agcgtgcata
     5521 ctagtcacca gaaattttgg gtagaatgtc tggaacgtta tctggtggga atgtgcgctg
     5581 ccgccacaca agacgatgtg gtgaaccaca ctgttttgtt gctttgcagc gaaatacaga
     5641 acatcggaaa gactacgttt atcaacaacc tgctgccgcc cgaattgcgc gcctatcttt
     5701 ctaccggtct tatcaacccg aacaacaagg atgatctggc caaaatagcg caggcgatgc
     5761 ttatcaatct ggatgagttt gaagggatga acggacggga actgaatatc ttcaaggatt
     5821 tggttacccg caaagtaatc agcatccgcc tgccatacgc gcggcgttca cagaactttc
     5881 ctcacacggc ttcttttgcc ggcacatgca attatcagga agtgcttcac gacaccactg
     5941 gtaaccgccg ttttctctgc tttcatgtcg actcgatgga atttatcaaa ataaactacg
     6001 cgcagttgta tgcacaaatc aagtatctac tcaacaagcc ggggtatcag tattggttca
     6061 cccagtcgga aaactcacgg attgaagaga acaacgagga tttcattttc cactcaccgg
     6121 aagaagaact ggtgttgaca catatccgca aaccggaacg tttcgaaaag gtgcattatc
     6181 tgacggtcac tgaaattgcg gaattgattc gcgaacggac ggggtatcaa tattctcacg
     6241 gaaccaaagc ccaactagga aaagtgatgt ccaaacacgg cttcgaattt cataagggaa
     6301 aaaacgggag aagatacact gtattcatta ttgatacgga acaggtgaaa tcaaacaggc
     6361 tatatgaata agatttcatc tccttcgcac gcaaaaaggt gagggttggt gactgtctct
     6421 tatacacatc tgacgctgcc gacgaataga gaggtgta
//
