LOCUS       c00336_gnlPro..         5378 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NECHEGGL_336.
ACCESSION   c00336_gnlPro..
VERSION     c00336_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 02:09:36
            Original ID  :: gnl|Prokka|NECHEGGL_336
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5378
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5378
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1138:5367]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1139..5367
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..5378
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5378
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             complement(2..328)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg336_1"
                     /sec_met_domain="IlvN (E-value: 3.5e-21, bitscore: 64.2,
                     seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQLNFGGTTETVVIRDEFPLEKAREVLKNETIAVIGYGVQGPGQ
                     ALNLRDNGFNVIVGQREGKTYDKAVADGWVPGETLFGIEEACEKGTIVMCLLSDAAVMS
                     VWPTI"
     CDS             complement(396..1139)
                     /locus_tag="ctg336_2"
                     /transl_table=11
                     /translation="MSEENKIGTYQFVAEPFHVDFNGRLTMGVLGNHLLNCAGFHASDR
                     GFGIATLNEDNYTWVLSRLAIELDEMPYQYEDFSVQTWVENVYRLFTDRNFAIIDKDGK
                     KIGYARSVWAMINLNTRKPADLLTLHGGSIVDYVCDEPCPIEKPSRIKVTSDQPMATLT
                     AKYSDIDINGHVNSIRYIEHILDLFPIELYKTKRIRRFEMAYVAESYFGDELSFFCDEV
                     NANEFHVEVKKNGSEVVCRSKVIFE"
     CDS             complement(1139..1702)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg336_3"
                     /sec_met_domain="ALS_ss_C (E-value: 3e-19, bitscore: 57.9,
                     seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDKTLYTIIVHSENIAGLLNQVTAVFTRRQINIESLNVSASSIK
                     GVHKYTITALTDKDTIEKVVKQIEKKIDVIQAHYFTEDEIYFHEIALYKVSTPAFQETP
                     EASKLIRRYNARVVEVNPVFSIVEKNGMSEDITSLYGELKALNCVLQFVRSGRVAITTS
                     CFERVNEFLDGREAMYNQSKNQQE"
     CDS             complement(1759..3453)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg336_4"
                     /sec_met_domain="TPP_enzyme_C (E-value: 5.4e-48, bitscore:
                     151.4, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDLITGAEALMRSLEHQGVTTIFGYPGGSIMPVFDALYDHQNIL
                     NHILVRHEQGAAHAAQGYARVSGDVGVCLVTSGPGATNTVTGIADAMIDSTPIVVIAGQ
                     VGTGFLGTDAFQEVDLVGITQPITKWSYQIRRAEDVAWAVARAFYIAHSGRPGPVVLDF
                     AKNAQVEKTKYEPTKVDFIRSYVPVPDTDEETVQAAAELINNAERPLVLVGQGVELGNA
                     QSELREFIEKADMPAGCTLLGLSALPTEHPLNKGMLGMHGNLGPNINTNKCDVLIAVGM
                     RFDDRVTGNLATYAKQAKVIHFDIDPAEVNKNVKVDVAVLGDCKETLASVTKLLKKNTH
                     TEWIDSFKEYEKVEEEKVIRPELHPATNSLSMGEVVRAVSDATHHEAVLVTDVGQNQMI
                     SARYFKYTKERSIITSGGLGTMGFGLPAAIGATFGAPERTVCVFMGDGGLQMNIQELGT
                     IMEQKAPVKIICLNNNYLGNVRQWQAMFFNRRYSFTPMLNPDYMKIASAYDIPSKRAFT
                     REELKEAIAEMLATDGPFLLEACVVEEGNVLPMTPPGGSVNQMLLEC"
     CDS             3494..3643
                     /locus_tag="ctg336_5"
                     /transl_table=11
                     /translation="MTVENEWFTIIALSAAQLTITYQLYYIILTAPLSAELTMLAYAFR
                     LFGT"
     CDS             complement(3568..5367)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg336_6"
                     /sec_met_domain="ILVD_EDD (E-value: 2.1e-217, bitscore:
                     711.4, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKQLRSSFSTQGRRMAGARALWAANGMKKNQMGKPIIAIVNSFT
                     QFVPGHVHLHEIGQLVKAEIEKLGCFAAEFNTIAIDDGIAMGHDGMLYSLPSRDIIADS
                     VEYMVNAHKADAMICISNCDKITPGMLMAAMRLNIPAVFVSGGPMEAGEWNGQHLDLID
                     AMIKSADESVSDQEVANIEQNACPTCGCCSGMFTANSMNCLNEAIGLALPGNGTIVATH
                     ENRTQLFKDAAELIVKNAKLYYEEGDESVLPRSIATRQAFLNAMTLDIAMGGSTNTVLH
                     LLAIAHEAEVDFKMDDIDMLSRKAPCLCKVAPNTQKYHIQDVNRAGGIIAIMDELAKGG
                     LIDTSVRRVDGMSLAEAINEYSITSPNVSEKAIKKYSSAAGNRFNLVLGSQGMYYKELD
                     KDRANGCIRDLEHAYSKDGGLAVLKGNIAQDGCVVKTAGVDESIWKFTGPAKVFDSQEA
                     ACEGILGGRVVSGDVVVITHEGPKGGPGMQEMLYPTSYIKSRHLGKECALITDGRFSGG
                     TSGLSIGHISPEAAAGGNIGKIVDGDIIEIDIPARKINVRLTDEELAARPMTPVTRDRY
                     VPKSLKAYASMVSSADKGAVRII"
ORIGIN
        1 tgatagtagg ccatacagac attactgctg cgtcagacaa caagcacatt acgatagtac
       61 ctttctcgca agcttcttca atgccgaaca aagtttcgcc cggaacccat ccgtctgcta
      121 ctgctttatc gtatgttttt ccttcacgtt gaccaacgat tacattgaaa ccgttatcac
      181 gcaagttcag tgcctgtcca ggtccctgta cgccataacc gattacagca attgtttcat
      241 ttttcaatac ttcacgagct ttttccaatg gaaattcgtc acggattact acagtttcag
      301 tagtacctcc aaaattcaac tgtgccattt tctttttgtt tttagttttt gatgacttac
      361 gtcatccgtt attaattaat tttgtattct cacatttatt caaatatcac tttcgaacgg
      421 caaacaactt cgctgccatt cttctttact tctacgtgaa actcgtttgc attcacttca
      481 tcgcagaaga aagagagctc atcaccaaaa taactttcgg ctacgtacgc catctcgaaa
      541 cggcggatac gtttggtctt gtatagctct atcgggaaca aatcaaggat atgctcgatg
      601 taacggatgc tgttcacatg accgttaata tcaatatcac tgtattttgc cgtcagggta
      661 gccattggct ggtcgcttgt cactttaata cgtgaaggct tttcaatagg acaaggttcg
      721 tcacagacat aatctacgat acttcctccg tgcaaggtca gtaaatcggc cggtttgcgg
      781 gtattgagat taatcatcgc ccatacggaa cgcgcataac caatcttttt cccatcctta
      841 tcaataatag cgaagttacg gtcagtaaac aagcggtaaa cattttccac ccatgtttga
      901 actgaaaaat cctcatattg ataaggcatt tcatccaact caatggccag acgcgaaagt
      961 acccaagtgt aattatcctc gttcagtgtt gcaataccaa agccacggtc acttgcgtga
     1021 aaaccggcac aattcaacaa atgatttccc agtacgccca tagtcagacg tccgttaaag
     1081 tcaacatgga acggttctgc cacaaactga taagttccta ttttattttc ttcactcatt
     1141 attcttgttg gtttttactc tggttataca tggcttcccg tccatcgaga aactcgttca
     1201 cccgttcaaa gcaactggtg gtaatcgcta cgcgtcccga ccgtacaaac tgcaaaacac
     1261 aatttaaagc cttcagttca ccgtagagtg aggttatatc ttcgctcata ccatttttct
     1321 cgacaataga aaataccgga ttaacttcca ctacacgggc attgtatctg cggatcaact
     1381 tggatgcttc cggagtttcc tgaaaagcag gagtagatac cttatatagt gcaatctcat
     1441 ggaagtatat ttcatcctcg gtgaaataat gtgcctgaat cacatcgatc ttcttttcga
     1501 tctgtttcac taccttctca atggtgtcct tatccgtcaa ggctgtaatc gtatacttgt
     1561 gtacaccttt aatagaagaa gcagatacat tcagactctc gatgttgatt tgccgacgag
     1621 taaatacagc agttacctga ttcagcaatc cggctatatt ttcggaatga actattatcg
     1681 tatataatgt cttgtcactc ataatcctaa attttaattg cgaactttca atcttccatt
     1741 ctcaatattc aacttcccct agcattccaa cagcatctga ttgaccgaac cacccggagg
     1801 agtcatcggc aaaacattgc cttcttccac tacgcacgct tcgagcagga acggaccatc
     1861 tgtcgccagc atttctgcaa tagcttcttt cagttcttca cgggtgaaag cacgtttgga
     1921 aggaatatca taagccgaag caatcttcat gtaatccgga ttcaacatcg gggtaaacga
     1981 atagcgacgg ttgaaaaaca tagcttgcca ctgacgtacg tttccgaggt aattattgtt
     2041 caggcagata atcttcaccg gagccttctg ctccataatc gtacccagtt cctgaatatt
     2101 catttgcagt ccgccatctc ccataaatac gcatacggta cgctccggtg ccccgaaagt
     2161 tgccccgata gctgcgggaa gcccgaagcc cattgttcca agtcctccgg aagtaatgat
     2221 actgcgttct ttcgtatatt taaagtaacg tgcagatatc atctgatttt gacctacatc
     2281 tgttaccaaa acagcttcat ggtgagtagc atcactaacc gcacgtacca cttcccccat
     2341 actcaacgag tttgtggcag gatgaagttc aggacggatt actttttcct cctctacttt
     2401 ctcatattct ttgaagctat caatccattc ggtatgagtg ttctttttca ggagcttcgt
     2461 cacagaagcc agtgtttcct tgcaatctcc caatacagct acatctacct ttacattctt
     2521 attaacttcc gcaggatcga tatcaaaatg aatcactttt gcctgtttgg cataagtagc
     2581 caaattaccg gtcacacggt cgtcaaaacg cataccgaca gcaatcagca catcacattt
     2641 attggtattg atattcggtc ccagattgcc gtgcataccc aacatacctt tattcaacgg
     2701 atgttctgtc ggcaaagccg aaagtccgag cagcgtacaa cccgcaggca tatccgcttt
     2761 ttcgataaac tcgcgcaatt cgctctgtgc atttcccagt tcaacacctt gtccaaccaa
     2821 tacaagcgga cgttcagcgt tattaatcag ttcggcagca gcctgtacag tctcctcgtc
     2881 cgtatcggga acaggaacat aactacggat aaaatcaact ttcgttggtt cgtatttcgt
     2941 tttctccact tgcgcatttt tggcaaagtc cagtaccacc ggccccggac gaccactatg
     3001 ggcaatgtaa aacgcacgtg ctaccgccca ggctacatct tccgcacggc gaatctgata
     3061 actccattta gtgattggct gggtaatacc taccaagtca acttcctgaa aagcatcggt
     3121 cccaagaaag cctgttccta cctgtccggc aatcaccacg ataggcgtac tatcgatcat
     3181 cgcatccgca atgcctgtta ccgtattggt tgctccggga ccgctcgtca ccaggcaaac
     3241 acctacatca cccgatacac gggcataacc ttgtgcggca tgggcggctc cctgctcgtg
     3301 acgaaccaag atatggttca atatattttg atgatcgtac aaggcatcga ataccggcat
     3361 aatagaacca ccggggtaac caaagatggt agtcactccc tgatgttcca aggagcgcat
     3421 caaagcctct gcacctgtta ttaaatcttt catattagtt gatagttgat aaatgataaa
     3481 tgatagttga gaaatgacag ttgaaaatga atggtttaca atcattgcgc taagtgctgc
     3541 gcagctaact attacctatc aactatatta tataattctc actgctccct tatcggcaga
     3601 gcttaccatg ctggcatacg ccttcaggct cttcggtaca taacggtcac gagtgacagg
     3661 agtcatcgga cgggccgcca gttcttcatc cgtcagtcgc acgttaatct tccgggccgg
     3721 aatatcgatt tcgataatgt ctccgtctac aatttttccg atattgcctc cagctgccgc
     3781 ttcaggagaa atatgtccga tactcaatcc ggaagttcca ccactgaaac gtccgtcggt
     3841 aatcaaagca cattcttttc cgagatgacg agatttgata taagaggtag gataaagcat
     3901 ttcctgcata ccgggaccac ccttcggacc ttcgtgcgta atgacgacta catcgccact
     3961 gacgactcgg cctccgagga tgccttcaca ggctgcttct tgcgaatcaa atactttggc
     4021 aggtccggta aatttccaga tactttcgtc tacacctgct gtttttacca cacaaccgtc
     4081 ttgggcaata ttgcctttca atacggccaa tccaccgtct ttgctataag catgttccaa
     4141 atcacggata catccgtttg cacggtcttt atccagttcc ttatagtaca tgccctgtga
     4201 accaagtacg agattgaatc tatttccggc tgcactcgaa tattttttaa ttgccttttc
     4261 gcttacattc gggctggtga ttgaatattc attaatagct tccgccaatg acattccgtc
     4321 tacccgtcga acagatgtat ctatcagacc gccttttgcc agttcatcca tgatagcaat
     4381 aataccaccg gcacggttta catcctgaat atgatatttc tgtgtattgg gagccacctt
     4441 gcagagacaa ggagccttgc gggaaagcat atcgatatca tccatcttga aatctacctc
     4501 tgcttcatga gcaatagcca acaggtgaag aactgtatta gtagaaccac ccattgctat
     4561 atccagcgtc atggcattca agaaagcctg acgggtagca atgctacgag gaagcacact
     4621 ttcgtctcct tcttcataat ataactttgc attttttacg atcagttctg ctgcatcttt
     4681 gaaaagctgc gtacgattct cgtgtgtagc tacgatggtt ccgtttccgg gaagagccag
     4741 tccgattgct tcattcaggc agttcattga attggcggta aacataccgg aacaacatcc
     4801 gcaagtagga caagcatttt gctcgatatt cgctacttcc tgatcgctga cactttcatc
     4861 agccgattta atcatcgcgt cgatcaaatc caggtgctga ccattccatt ctccggcttc
     4921 catcggaccg cccgatacaa atacggcagg aatattcaaa cgcatagccg ccatcaacat
     4981 tccgggagta atcttatcgc aattgctgat acatatcatt gcatctgcct tgtgcgcatt
     5041 aaccatatat tctacactgt ctgcaatgat gtcacgtgaa ggaagcgagt aaagcatacc
     5101 atcatgtccc attgctatac cgtcgtcgat agctattgta ttgaattccg cagcgaagca
     5161 tcccagcttc tcaatctctg ccttcaccag ctgaccgatt tcatgcagat gcacatgtcc
     5221 cggaacaaac tgggtgaacg aattgacgat agcaataatg ggtttaccca tctgattctt
     5281 cttcatgccg ttggctgccc ataacgcacg ggctcccgcc atccgacggc cttgtgtact
     5341 aaacgaactg cgtaattgct ttttcatttc aatctatc
//
