LOCUS       c00258_gnlPro..         7065 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LNOAIPPO_258.
ACCESSION   c00258_gnlPro..
VERSION     c00258_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 00:40:37
            Original ID  :: gnl|Prokka|LNOAIPPO_258
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7065
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7065
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3977:6950]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3978..6950
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7065
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7065
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(132..2249)
                     /locus_tag="ctg258_1"
                     /transl_table=11
                     /translation="MDHFTIFQDFYRVIAEMTEEEIVSAISNGTYRETVEDVRRIFAEQ
                     GEKAANEKKIELPEITFSANYRGGRSNATLVKYLGYIVVDIDHQTQEALARILALAKKC
                     AHTRIAFISPKGMGLKIVVRVCRTDGTLPETIQEIEDFHHAAYHKIASFYAQLCGVEVD
                     TSGQDVSRTCLFSYDPDIYFNPDATAVIVEQPPMFFKSQKKKRASGKKKKTTAPDNNPL
                     TEQVALNYHSSHASLMVTLNYYHNKSEKYVTGNRNNYLHCLACMYNRYGVPQEEAAAFI
                     KSQFTDLPADEMDALIGSAYGHNEEFDTRKLNSTQKRILQIEQHIKENYDTRYNEVLHI
                     MEYRRRKTDTEQPEPFHILDEMMENSIWMEMNELGYSCTVKTIQNLIYSDFSSTYHPIR
                     EYLDSLPEWDGTDYIGILANSVHTSHQKFWVECLERYLVGMCAAATQDDVVNHTVLLLC
                     SEIQNIGKTTFINNLLPPELRAYLSTGLINPNNKDDLAKIAQAMLINLDEFEGMNGREL
                     NIFKDLVTRKVISIRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHVNSME
                     FIKINYAQLYAQIKYLLNKPGYQYWFTQSENSRIEENNEDFIFHSPEEELVLTHIRKPE
                     RFEKVHYLTVTEIAELIRERTGYQYSHGTKAQLGKVMSKHGFEFHKGKNGRRYTVFIID
                     TEQVKSNRLYE"
     CDS             complement(2337..2567)
                     /locus_tag="ctg258_2"
                     /transl_table=11
                     /translation="MYYPDQPFHIRTYKKSELAHLYNPNVCLKVALQILRRWIVYNLPL
                     LQELEQEGYRARNRLLSPRQVATIIRYLGEP"
     CDS             2761..3264
                     /locus_tag="ctg258_3"
                     /transl_table=11
                     /translation="MAMTVTYSVVPRKNPAKKDESAKYYAQAQASGELDFEELCEGITS
                     RSTCTETDVRAAISGILYEAKRALKAGRIVRLGDLGSLQIGLNSEGAVSVKEFSSSLIT
                     AAHIIFRPGKTLADITKILSYQQVTTRAVAQTGGSGNEGEDDDKGSGGGSDGGGSGEAP
                     DPAA"
     CDS             3261..3452
                     /locus_tag="ctg258_4"
                     /transl_table=11
                     /translation="MITLILDQMKEIVTKILDVIMFLVPFFGKRKRNRIVREVRFNATH
                     KEVCNVKTTEREKDDEKD"
     CDS             3439..3885
                     /locus_tag="ctg258_5"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDRDFTAKDVDTAHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDENGKAADTRTSRQKEAMHRLVSELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIEESES"
     CDS             complement(3978..4706)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg258_6"
                     /sec_met_domain="Radical_SAM (E-value: 3e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAVEVFKEYFTTVVVN"
     CDS             complement(4722..6950)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg258_7"
                     /sec_met_domain="Gly_radical (E-value: 3.4e-47, bitscore:
                     148.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1172.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLRNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     NFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             complement(6962..7063)
                     /locus_tag="ctg258_8"
                     /transl_table=11
                     /translation="FYVEKHKIEHIYADYMKKRCNFVRFKEKDNYNL"
ORIGIN
        1 acactcgact agtcgtcggc agcgtcagat gtgtataaga gacaggaata gaaataagaa
       61 atataaaaat gaaaagttgc aaaagtaccc tcaccaaccc tcaccttttt gcgtgcgaag
      121 gagatgaaat cttattcata tagcctgttt gatttcacct gttccgtatc aataatgaat
      181 acagtgtatc ttctcccgtt ttttccctta tgaaattcga agccgtgttt ggacatcact
      241 tttcctagtt gggctttggt tccgtgagaa tattgatacc ccgtccgttc gcgaatcaat
      301 tccgcaattt cagtgaccgt cagataatgc accttttcga aacgttccgg tttgcggata
      361 tgtgtcaaca ccagttcttc ttccggtgag tggaaaatga aatcctcgtt gttctcttca
      421 atccgtgagt tttccgattg ggtgaaccaa tactgatacc ccggcttgtt gagcagatac
      481 ttgatttgtg catacaactg cgcgtagttt attttgataa actccatcga gttgacatga
      541 aagcagagaa aacggcggtt acctgtggtg tcgtgaagca cttcctgata attgcatgtg
      601 ccggcaaaag aagccgtgtg agggaagttc tgtgaacgcc gcgcgtatgg caggcggatg
      661 ctgattactt tgcgggtaac caaatccttg aagatattca gttcccgtcc gttcatccct
      721 tcaaactcat ccagattgat aagcatcgcc tgcgctattt tggccagatc atccttgttg
      781 ttcgggttga taagaccggt agaaagatag gcgcgcaatt cgggcggcag caggttgttg
      841 ataaacgtag tctttccgat gttctgtatt tcgctgcaaa gcaacaaaac agtgtggttc
      901 accacatcgt cttgcgtggc ggcagcgcac attcccacca gataacgttc cagacattct
      961 acccaaaatt tctggtgact agtatgcacg ctgttggcaa ggataccgat ataatccgtt
     1021 ccgtcccatt caggcagcga gtctaaatac tcgcgaatag gatggtaggt gctgctgaag
     1081 tcggagtaga tcagattctg aatcgtcttg acggtgcacg aatagcccag ttcgttcatc
     1141 tccatccaaa tgctgttttc catcatttcg tcgagaatat ggaaaggttc cggttgctcc
     1201 gtgtccgttt tccttctgcg atattccatg atatgaagca cttcgttgta gcgtgtatcg
     1261 taattttctt tgatatgttg ctcaatctgt aatattcgct tctgcgtact attcagcttg
     1321 cgggtgtcga attcctcatt gtgtccgtaa gcactcccga tcagtgcgtc catttcatcc
     1381 gcgggaagat cggtgaactg gctcttgata aaagctgccg cctcttcctg ggggacgccg
     1441 tagcgattgt acatacaagc caggcaatgc aggtagttgt tccggttgcc ggtgacgtac
     1501 ttttcggatt tattgtgata atagttcaga gtcaccatta aggaagcgtg cgaggaatgg
     1561 tagttgagtg ccacttgttc ggtgagggga ttgttgtctg gtgctgtcgt ctttttcttt
     1621 ttgccggatg cccttttctt cttttgagac ttgaagaaca tcggtggctg ttccacgatg
     1681 acagctgtgg catcgggatt gaaataaata tccggatcgt aagagaacag gcaagtgcgg
     1741 ctgacatcct gtccggaagt gtcgacctca actccgcaga gttgggcata aaaggaagcg
     1801 attttgtgat aagcggcatg atggaaatct tcaatttcct gaatcgtttc gggcaatgtc
     1861 ccgtccgtcc ggcatacacg taccacgatt ttcagtccca ttcctttggg actgatgaaa
     1921 gcgatgcggg tgtgggcaca cttttttgcc agtgcgagga tgcgtgccaa tgcttcctgt
     1981 gtctgatggt cgatgtctac cacgatgtat cccagatact tgacgagagt tgcgtttgaa
     2041 cgtcctcccc ggtagttggc tgaaaaggtg atttccggta gttctatttt cttttcgttg
     2101 gctgctttct cgccttgttc cgcgaagatt cggcgcacgt cctccactgt ttcccggtat
     2161 gtgccgttgc tgatggccga tacgatttct tcttctgtca tttcggctat gacgcggtaa
     2221 aagtcttgaa agatggtaaa atgatccatg ttggtttgtt ctttttaatt tgcggttgca
     2281 aagaaatttg tttgtaaaat aatagaatgg aacttttcgt ttttttgatt aagttcctag
     2341 ggttctccta aatagcgtat gatcgttgcc acttgccggg gagagagaag ccggttgcgg
     2401 gcacgatagc cttcttgttc gagttcttgc aggagcggaa ggttgtagac aatccaacga
     2461 cgtagtattt gtaaggctac tttaaggcat acgttggggt tgtaaagatg agctagttcc
     2521 gactttttat aagtgcggat atgaaacggc tgatccggat aatacatgat gaatgatatt
     2581 tggtttatgc tataaagtta ctgaaaatta atggtttaat aaaagaataa cgtcattatt
     2641 attcatagag tatcaggaaa cttcgttggg gaatccggag ggtgaaaaat gtgttatctt
     2701 tatgtcgtta acaaataagg aacgcgttcg aaatgtgtga aatcttaaaa ttattttatt
     2761 atggcaatga cagtaactta ttcagtagtg ccgcgcaaga atcctgccaa aaaggatgag
     2821 tcggcaaagt attacgcgca ggcacaggct tcgggagagt tggattttga agagttgtgc
     2881 gagggaatca ccagtcggtc tacctgtacg gagacagatg tgcgggccgc catttcgggt
     2941 attctttatg aggcgaagcg tgcgttgaaa gcgggaagaa tagtccggct gggtgatttg
     3001 ggcagtttgc agatcgggtt gaacagtgaa ggagctgtgt cggtcaagga gttttcaagt
     3061 tcgctgatta cggctgccca tattattttc cgtcccggaa agacgttggc ggacatcacg
     3121 aagattctta gctaccagca ggtaacgacg cgtgcggtgg cccaaacggg cggtagcggc
     3181 aatgaaggag aagacgatga caaaggttcc ggtggcggtt cagacggtgg tggaagcgga
     3241 gaagctccgg acccggcagc atgataactt taatattgga tcaaatgaaa gaaattgtaa
     3301 ctaagatttt ggatgtaatt atgttccttg tgcctttctt cggaaaaaga aagcggaaca
     3361 gaattgtgag agaggtgcgc ttcaatgcca cccataagga agtgtgtaat gtgaaaacga
     3421 cggaaaggga gaaggacgat gagaaagatt agtctgattg tgattcactg ctctgctacc
     3481 cgtgtcgatc gcgacttcac ggcgaaggat gtggacactg cccatcgctt tcggggcttt
     3541 tcgtgttggg ggtatcatta ttatatccgc aaatcgggac agatagaacc gatgagggat
     3601 gaagatacgg tgggtgctca tgcacgcggt tttaatgcga ttagtttagg ggtgtgctat
     3661 gaaggcgggc tggatgagaa cggaaaggca gcggatacac gtacttcccg ccagaaagag
     3721 gcgatgcatc gtctggtaag cgagttgttg cagcgttatc cggatgcaaa ggtggtcggt
     3781 caccgggact tgagccctga cactaattat aatggaattg tagacccctg ggaacggatc
     3841 aaagagtgtc cctgtttcga ggtgatcgaa gaatctgaga gttgagaatg gagagttgag
     3901 agttggctgc gcactgttat actgcatagt taactctcaa ttctcaactc tcaattctca
     3961 attctcaact tcttcaacta attgaccacc acagtagtga aatattcttt aaatacctcc
     4021 acggcttttt caagttgttc gggggtattt tcttttacgt ctttcagctt gtattcttgc
     4081 tccatcgctt catacttatg tacgcctaat gtatgataag gcaagatttc cactcgttgc
     4141 accatcttat attttcccag agcctctcct aaacggcgga tgtcttcctc gaaatcgctg
     4201 tatccgggca ctaatacata acgcagccag aaaggtttct cgttttcttc cagccatgct
     4261 gccgtgcgga tggtttgctc gttacttctt ccggtcagtg cctgatggcg tgccggattg
     4321 aactctttaa tatccagcaa tacaagatcc gtcagtttga aaagttcttc tacttcttcg
     4381 ttccagatac caccgttgct gtctatgcat acatgtattc ccttttcttt cagttcacgc
     4441 actaaaggaa caagtgcttt ggcctgaaac gtaggttctc ctccggagaa agtgactcct
     4501 ccgcgtttcc cgaaaaaagg acgttggctc atagccatgc ggacgatttc ttccggtgga
     4561 gtaggtgtgc ctcctttccc ggctattgtg tcagggttgg cgcaatacag gcagcggaaa
     4621 ttgcatcctt gaagaaaaac gacgagccgt aagcccggcc cgtcgaatgt tcccatactt
     4681 tcgtatgaat gtacgtttat cgtcatattg agtagatctg attacatacg ttcgtggaag
     4741 ctacggctga taacttccaa ttgatgttcg cggctcaact tcacgaagtt tacggcataa
     4801 ccggaaacac ggatggtgag ttgcggatat ttttccggat gttccatggc gtcgtaaagc
     4861 atttcacggt tcagtacgtt cacgttcagg tggtgagcgc ctttggtgaa gtaaccatcc
     4921 atcattgtca ccagattttc tacgcgatct tcttcagtag ctcccagtga tttcggaacg
     4981 atagagaagg tgttgctgat accgtcttgt gagtcgcggt aacgtaattt cgctacggaa
     5041 ctcaaagagg cgatagcacc gttcttgtca cgtccgtgca tcggattggc tcccggagca
     5101 aaagcgacac ctttggcgcg gccgtcggga gtagcacctg tcttcttgcc atacatcacg
     5161 ttggaagtga tggtgagcaa agacagagta ggacgggcat tcttgtaaac cggaagtttc
     5221 ttcaattctt cgctgaagaa atagaccagg tcaacaccca gatggtctac cttgtcgttg
     5281 tcattaccga agcaagggaa ttcgccttga atatcgaaac cttctgtcag accgatgtcg
     5341 ttgcggcgtg cagtcacttt ggcatatttg atggccgaca gtgaatcgag ggcgatagaa
     5401 agtccggcta caccgtaagc aaggttgatg cgtgggttgg tatctacaag agccatttgg
     5461 gctttttcgt agtaatactt atcatgcata taatggatga tgttcatcgc ttcgttgtaa
     5521 acacgggcga tttctgtcaa taccttcttg tagttgctca ttacttcttc gaagttcagt
     5581 gtgtcgctgg ttagtacggg gatatttttc accattaccg taccggtgtt ttcgcaacgt
     5641 ccgccgttga tggcaagcag cagggctttt gccaggttgc aacgtgcgcc gaagaattgg
     5701 atttgttttc cgatttcctg ataagatacg cagcacgcga ttccgtagtc gtcagactgg
     5761 cgcacttcac gcatcaggtc gtcgttttca tattggatgg aagaagtgtc gatagatact
     5821 tttgcgcaga attctttgaa tccttccgga agttccgggc tccacaatac agtcaggttc
     5881 ggttccggtg aagggccgag gttgtagagt gtttgcagga agcggaaaga agtctttgtc
     5941 acctttgtgc gtccgtcgtt gaggcgtccg cccagagatt cggttaccca tgtcgggtcg
     6001 cccgcaaaga tgtcgttgta agattgcata cgcaggtggc gcaccatacg cagtttgatg
     6061 acaaactggt cgatcagctc ctgtgcgaaa gattctgtga tagttccttt gctcaattca
     6121 tattccatat aaatgtcgag gaacgaagag acgttaccca gtgacatggc agcaccgtct
     6181 tgttctttta cggcagcaag gtaagccatg tacacccatt gtactgcttc ttgtgcggtg
     6241 taggcaggac ggctcaagtc gagtccgtag tattcgccca ttaccttcat gtccttcaat
     6301 gccttgattt gctctgccac ttcttcgcgc aggcggatgc gggcttcggt catcggaccg
     6361 gtaaggttac gcaaatcttc tttttttgcc tcaatcagtc ggtcgatacc atagagagcc
     6421 atacggcggt agtcgccgat gatacgtccg cgggcatagt tgtcaggaag tccggtgagg
     6481 aatcccagag aacggaacga acggatttct tcggtatata cgtcaaatac tccgtcattg
     6541 tgcgtcttgc ggtagtgagt gaatatatct tttacacggt cgtccacttc cacgccgttt
     6601 tcgtgacagg ctttgctcac cacgttgata cctccgaaag gtttgattgc acgtttcaga
     6661 agttcgtctg tttgcagacc gacgatcagt tcgttttctt tgtcgatata tccggcttta
     6721 tgagaagtga tggttgagac ggtgacatta tccagtgagc gtactccgtt attggctctt
     6781 tcttctgcca gtgcttcgag gcagcggttc catacagctt tagtgcgttc ggtaggtcct
     6841 tcgaggaaag aagcatctcc ataatacgga gtgatgttgt gactaacgaa atctcttacg
     6901 ttgatttctg tgctccaaag accgtcttta aagatcttat ttaattccat aaatgataaa
     6961 attagaggtt gtagttatcc ttttctttaa agcgcacaaa gttacaacgt tttttcatat
     7021 aatcagcata aatatgctct attttatgct tttcaacata aaata
//
