LOCUS       c00297_gnlPro..         5612 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AIOMLBID_297.
ACCESSION   c00297_gnlPro..
VERSION     c00297_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 00:53:45
            Original ID  :: gnl|Prokka|AIOMLBID_297
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5612
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5612
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:3492]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..3492
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..5612
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5612
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             2..1069
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg297_1"
                     /sec_met_domain="TPP_enzyme_C (E-value: 6.5e-52, bitscore:
                     163.8, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="CLVGQGVILGGAEEELKEFLLKNDIPAGSTVLGLSALPSDFPLNK
                     GMLGMHGNVGPNRKTNECDVLIAIGMRFDDRVTGDLKTYAKQAKIIHLDIDNSEIGKNV
                     PVDVKVLGNAKHTIPMITALLEERKRPEWNAEFEPDEKEEYNKVIEKELYPTEGQLKMG
                     EVVRKVSEATDNDAILVTDVGQNQMMGVRYFKYKQTRSVVTSGGLGTMGFGLPAAIGAK
                     LGAPDRTVCFFTGDGGIQMTIQELGTIMQENLNVKIIVLNNNFLGMVRQWQELFFNERY
                     SNTIMKNPDFVAIAKAFGIASRAVDKREDLDEAIAEMLNHDGAYMLVANVETCGMVYPM
                     VPAGGSVTNMILGDK"
     CDS             1082..1636
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg297_2"
                     /sec_met_domain="ALS_ss_C (E-value: 5.6e-19, bitscore:
                     56.7, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKQLYTVIIFSENTVGLLNQITVIFTRRQLNIETLSVSPSAIK
                     GIHKFTITTFADEDTIDKVVKQIDKRVDILKAYYNTDEDLVYQEIALYKLSTELFIKMG
                     TVEDLIRKYNARILEMNEDCVVLEKSGHYDETQALFKELSETIGVLQFIRSGRVAITKS
                     KVERLSDMLSAMEKKLQEKHV"
     CDS             1673..2404
                     /locus_tag="ctg297_3"
                     /transl_table=11
                     /translation="MEKNKVGEFHFVTESYLMDFRGRITIPMIGTYLLHAASCHAADRG
                     FGYNDMTERHTAWVLSRLAIEMTSYPAMSESVTLYTWVDEVGKLFTSRCFELVNGEGKT
                     FGFARSIWAAIDMETRRPTLLDVEGLSAYITDRPCPIEKPGKIAPVENNTSGEPYRVKY
                     SDLDVNGHLNSIKYMEHLLDLFDISLFREKEISRFEIAYQSEGKYGMELTLHNQEVSPG
                     KYNMAICNDGKAISRAAVTWI"
     CDS             2446..3492
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg297_4"
                     /sec_met_domain="IlvN (E-value: 6.1e-49, bitscore: 154.2,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 1.4e-44, bitscore: 140.3,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVMNFGGVDENVVTREEFPLEKAREVLKDETIAVIGYGVQGPGQ
                     SLNLRDNGFNVIVGQRKGGKTWEKAVADGWVPGETLFDIEEACEKATIIQYLLSDAAQI
                     EVWPRIKKYLTPGKALYFSHGFGITYKERTNIIPPADVDVILVAPKGSGTSLRRMFLQG
                     RGLNSSYAIFQDATGKAWDRVIALGIGVGSGYLFETTFKKEVYSDLTGERGTLMGAIQG
                     LLLAQYETLRENGHEPSEAFNETVEELTQSLMPLFAENGMDWMYANCSTTAQRGALDWM
                     GPFHDATKPVFQKLYSEVACGNEAQRSIDTNSKPDYREGLEKELAALRESEMWRAGAVV
                     RKLRPENN"
     CDS             3994..5610
                     /locus_tag="ctg297_5"
                     /transl_table=11
                     /translation="MISVLLGAAGAIAANPTGPETMVVQQGNKVTGLVEDQMGPVAGAS
                     VVVKGTTIGTITDIDGKFTLDNVRRGAVIVISFVGLATQEITWNGQSILNVKLSDATQD
                     LDEVVVVAYGTAKKSTFTGSASVVKAEKLEKIMGSGFAEALQGMSAGVNVVNVQGNPGA
                     DARVEIRGIASMSGKADPLYIVDGMPYDGKLNQINPTDIESMTVLKDAAATSLYGSRAA
                     NGVVMITTKRGKSEKPRINFRGGWGTSDNAVKNPKKADPYQQLENTWYALYYDALLYDG
                     MNMQQAGDYASSQALTKQVKATKDSNGNLIYVTPFRHINENYVLHDGNGNPYMNPNLEY
                     VWDEDDWDVYKAIFSRKLRQDYSVDISGQANGGKTAYFFSGGYLDDQGYGNRQYYKRYS
                     FRTNLSTELFDWWKVAGSLSYSRHRQNLSGGASRAANFTTTLSSPWLRNEDNTGWVVSE
                     KTGERMYSYGKYTNNFFGAHILNNSGDYWDNPNDDSFDNNMGHILSAQVNTEFTLPFNL
                     KFRSALNIDDYWYRTMQYTSAV"
ORIGIN
        1 ctgtctggta gggcaggggg ttatcctggg aggagccgaa gaagaactga aagaattctt
       61 gttgaaaaac gatataccag ccggttctac cgtattgggc ttgtctgcat taccttcgga
      121 cttcccatta aataaaggta tgttggggat gcatggaaat gtcggaccga accgcaagac
      181 aaacgaatgt gacgtgctga ttgctatcgg tatgcgtttc gatgaccgtg taaccggcga
      241 cctgaagact tatgccaagc aggcaaagat tatccacctg gatatcgaca attcggagat
      301 cggaaagaat gtgccggtcg acgtgaaagt gctgggaaat gccaagcata cgattccgat
      361 gattacggct ttactggaag aacggaagcg tccggaatgg aatgctgaat tcgaaccgga
      421 tgaaaaagag gaatataata aggtaataga aaaagaactc tatcctaccg aaggccaact
      481 aaaaatgggc gaagtagtcc gtaaggtttc cgaagcaacc gataatgatg cgattctggt
      541 tacggatgta ggtcagaacc agatgatggg tgttcgctat ttcaaatata aacaaacacg
      601 cagcgtagtt acttcaggag gtttgggaac gatgggcttt ggccttcccg ccgccattgg
      661 agcgaagctg ggagcgcctg accgtacggt ctgtttcttt accggcgacg gtggtatcca
      721 gatgactatc caggagttgg gaactatcat gcaggaaaat ctgaatgtaa agatcatcgt
      781 tcttaacaac aatttcctgg gaatggtacg ccagtggcag gagctgttct tcaacgaacg
      841 gtattcgaat accatcatga agaatccgga ttttgtagcg atagccaagg cttttggtat
      901 tgcttcccgt gcggtagata aacgggaaga cttggatgag gcgattgctg aaatgttgaa
      961 tcacgatggt gcctatatgt tggttgccaa tgtggaaact tgtggtatgg tatatccgat
     1021 ggttccggcc ggaggaagcg tgacaaacat gattttgggt gataagtaag gaggagaaac
     1081 tatgaataca aaacaattat ataccgttat tatcttttcg gaaaacactg ttggtcttct
     1141 gaatcagata actgttatct ttacgcgccg gcaactgaac attgagacgc tttctgtttc
     1201 tccctctgct ataaagggaa tacataagtt tacaattact acctttgccg acgaggatac
     1261 gatcgacaag gtcgtaaaac agatcgacaa gcgtgtggat atcctgaaag cctattataa
     1321 tacggatgaa gacctggttt accaggaaat tgctctttat aaactgagta ccgaactctt
     1381 tatcaagatg gggacggttg aggatctgat ccgtaagtac aatgcccgta tccttgaaat
     1441 gaatgaggat tgtgtggtgt tggaaaagag tggtcattat gatgagacac aggctttgtt
     1501 taaagaattg agtgagacga tcggtgtcct gcaatttatc cgttccggac gcgttgctat
     1561 aacgaagagt aaggtagaac gtctgagcga catgttatct gcaatggaaa agaagttgca
     1621 ggaaaaacat gtctgagaat aataaggaaa cattttgtaa ataaagtaaa ttatggagaa
     1681 gaacaaagta ggagaatttc atttcgtaac agagtcttat ctgatggact tccggggacg
     1741 tattacaatc cctatgatcg gaacttattt gttgcatgct gcttcttgcc atgccgccga
     1801 ccgtggattc ggatataacg atatgaccga aaggcatacc gcctgggttc tttcccgtct
     1861 ggctatcgag atgacttctt atcctgctat gtctgaatct gtaactttat atacctgggt
     1921 ggatgaggtc ggtaagttgt ttaccagccg ttgctttgaa ctggtgaacg gcgaaggcaa
     1981 gacctttggc tttgcccgct ctatctgggc ggctatcgat atggaaaccc gccgcccgac
     2041 cttgctcgat gtggagggcc tgagtgctta tatcaccgac cgtccctgtc cgattgaaaa
     2101 accgggtaaa attgctccgg tggaaaacaa cacttccggc gaaccttacc gtgtgaagta
     2161 cagtgacctg gacgtaaacg gccacctgaa cagtatcaaa tacatggaac acctgctcga
     2221 cttgttcgat atcagcctgt tccgagaaaa agagataagc cgtttcgaaa tagcttatca
     2281 gtccgaagga aagtatggga tggagctgac tctccataac caggaagtat cccccggtaa
     2341 atataatatg gcaatctgta acgacggcaa agccatcagc cgcgctgccg ttacctggat
     2401 ctgaaaaaca aattatttta tatacatttt aatagtaaaa agaaaatggc agtaatgaat
     2461 tttggcggcg ttgacgaaaa cgtagtaacc cgcgaagaat ttccgttgga aaaggcaaga
     2521 gaagtattga aagacgaaac aatcgctgta ataggttacg gcgtacaagg tcctggccag
     2581 agcctgaacc tgcgcgacaa cggttttaac gtaattgtag gacaacgtaa aggtggtaag
     2641 acctgggaaa aggccgttgc tgacggttgg gtaccgggcg aaacactgtt cgatatcgaa
     2701 gaagcatgcg aaaaagcaac tatcatccag tatctgcttt cagatgctgc tcagattgaa
     2761 gtttggccgc gcatcaaaaa atacctgact ccgggtaagg ctttgtattt ctctcacgga
     2821 ttcggtatca cttacaaaga acgtacaaac atcatccctc ctgctgatgt agacgtaatc
     2881 ctggttgctc cgaaaggttc aggtacttca ctgcgccgta tgttcttgca gggtcgtggt
     2941 ctgaactcaa gctacgcaat cttccaggat gcaacaggca aagcatggga tcgcgtgatt
     3001 gcactgggta tcggtgttgg ttctggttac ttgtttgaaa caacattcaa gaaagaagtt
     3061 tattctgacc tgaccggcga acgtggtact ttgatgggag ctatccaggg attactgttg
     3121 gctcagtacg aaactttgcg tgaaaacggt cacgaacctt ctgaagcatt caatgaaaca
     3181 gtagaagaac tgactcagtc attgatgccg ttgttcgcag aaaacggtat ggactggatg
     3241 tatgcaaact gttcaacaac tgcacagcgt ggtgctttgg actggatggg cccgttccac
     3301 gatgctacaa aaccggtgtt ccagaagttg tatagcgaag ttgcctgcgg taacgaagca
     3361 cagcgttcta tcgacacaaa cagcaagcct gactatcgtg aaggtctgga aaaagaactg
     3421 gctgctcttc gcgaaagtga aatgtggcgt gcaggtgctg ttgttcgtaa acttcgtcct
     3481 gaaaacaact aataaatggt gatacattaa tagttaaaca ttttgtgata taaaaacggc
     3541 aatccggctt ttcgggttgc cgttttttta tggtttatgt aatgcgttaa tggttaaaaa
     3601 atgtttttga ttgcaactgt tattgatttt tcatcccaat atgtatgtca ttgtttgaat
     3661 gtggatttta gataaaaaaa tcaattaaat ggtggttaaa atatatttat tagagcaata
     3721 aatatacata attatgcttg tattaaataa tgataagtta tatttgctga tggagatact
     3781 gtagtttgta gagtggaaat atagaaggat gttgtgtgta atgatcagta tatctttgtt
     3841 ttagttaact ttattttatt gaaatgtatg ccagtcactt ttatcttaga aaagtgtaac
     3901 ctctaatgaa atgaacgcta ttgtgattaa ttttatttaa ttaaagactt gaatttagct
     3961 atgtggacca actcaaaact aattagatca ctaatgattt ctgtattatt aggtgcggca
     4021 ggtgcgattg ctgccaatcc tacaggtccg gaaacgatgg tagtacaaca gggtaataag
     4081 gtaaccggcc tggtagagga ccagatggga cctgtcgccg gagcttctgt tgtagtgaaa
     4141 ggaaccacta tcggtacgat aaccgatatc gatggaaagt ttacgctgga taatgttcgg
     4201 cggggagcgg ttattgttat ttcctttgtc ggcctggcca ctcaggagat cacttggaac
     4261 ggacaatcta ttttaaatgt aaaattaagt gatgcgacac aggacctgga tgaagtcgtt
     4321 gttgtagcct atggaacggc taagaaatca accttcaccg gatcggcctc cgttgtaaaa
     4381 gctgaaaaac tcgaaaagat catgggctcc ggctttgcgg aagcattgca ggggatgagt
     4441 gccggtgtga atgttgtgaa tgtacagggt aaccccggag ctgatgcccg tgtggagatc
     4501 cgtggtattg cttccatgtc cggaaaggcc gatccgttat atattgtgga cgggatgcct
     4561 tacgatggaa aactgaatca gatcaatcct acggacatag aatcgatgac ggtgctgaag
     4621 gatgcggcgg ccacttcgct ttacggttcg cgtgcagcaa acggcgtcgt aatgattaca
     4681 accaagcggg gtaaatcaga aaaaccgcgt attaacttcc gcggaggttg gggtacttcg
     4741 gataatgctg tcaagaatcc gaaaaaagcc gatccgtacc agcagctgga aaatacctgg
     4801 tatgccttgt attatgatgc ccttctttat gacggcatga atatgcaaca ggcgggcgat
     4861 tatgcttcgt cacaggcatt gaccaaacag gtaaaggcta caaaagattc gaatggtaat
     4921 ctgatttatg tcactccgtt cagacatatc aatgaaaatt acgtattgca tgacggcaat
     4981 gggaatcctt acatgaatcc taatctggaa tatgtctggg atgaagatga ttgggatgtc
     5041 tacaaagcta tcttctcccg taaactgcgc caggattata gtgtggatat aagtggtcag
     5101 gccaatggtg gaaaaacagc ctatttcttc tccggcggtt atctggacga ccagggatat
     5161 ggtaaccggc agtattataa acgttattct ttccgtacca acctgagtac agagttgttc
     5221 gattggtgga aagtggccgg tagcctttct tattcgcgtc atcgccagaa cctttccggt
     5281 ggtgccagcc gtgcggctaa ctttacgacg actttgtctt caccctggtt acgtaatgaa
     5341 gataataccg gttgggtcgt ttctgaaaaa acaggggaac ggatgtacag ttacggtaaa
     5401 tataccaata acttctttgg tgcccatatc ctgaataact cgggtgatta ttgggataat
     5461 ccgaacgatg acagtttcga taacaatatg gggcatatcc tgtctgccca ggtaaataca
     5521 gagtttactc ttcctttcaa cctgaaattc cgttcggctt tgaatattga cgattactgg
     5581 tatcgtacca tgcaatatac ttccgccgta ca
//
