LOCUS       c00334_gnlPro..         5528 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NEOIGKPM_334.
ACCESSION   c00334_gnlPro..
VERSION     c00334_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 04:44:08
            Original ID  :: gnl|Prokka|NEOIGKPM_334
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5528
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5528
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:4370]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..4370
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..5528
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5528
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             2..193
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg334_1"
                     /sec_met_domain="ILVD_EDD (E-value: 8.3e-15, bitscore:
                     42.9, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="KIVDGDIIEIDIPARTINVRLTDEELAARPMTPVTRDRYVPKSLK
                     AYASMVSSADKGAVRLID"
     CDS             241..1938
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg334_2"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.9e-47, bitscore:
                     149.6, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKDLITGAEAMMRSLEHQGVTTIFGYPGGSIMPTFDALYDHQNT
                     LNHILVRHEQGAAHAAQGYARVSGKVGVCLVTSGPGATNTITGIADAMIDSTPIVVIAG
                     QVGTGFLGTDAFQEVDLVGITQPIAKWSYQIRRAEDVAWAIARAFYIASSGRPGPVVLD
                     FAKNAQVEKTKYEPTQQEFIRSYVPVPDTDEESVKAAAELINNAERPLVLVGQGVELGS
                     AQEELRIFIEKADMPAGCTLLGLSALPTDHPLNKGMLGMHGNLGPNINTNKCDVLIAVG
                     MRFDDRVTGNLATYAKQAKVIHFDIDPAEVNKNVKVDIAVLGDCKKTLAAVTGLLKKNR
                     HTEWVDSFKEYEAVEEEKVIRPELHPATDSLSMGEVVRAVSEATRHEAILVTDVGQNQM
                     ISARYFKYTRERSIVTSGGLGTMGFGLPAAIGATFGRPDRTVCVFMGDGGLQMNIQELG
                     TIMEQKAPVKIICLNNNYLGNVRQWQAMFFNRRYSFTPMLNPDYMKIASAYDIPSKRVF
                     SREELKAAIDEMLSTDGAFLLEACVVEEGNVLPMTPPGGSVNQMLLEC"
     CDS             1957..2520
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg334_3"
                     /sec_met_domain="ALS_ss_C (E-value: 3.3e-20, bitscore:
                     60.9, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDKTLYTIIVHSENIAGLLNQVTAVFTRRQINIESLNVSASSIK
                     GVHKYTITAWTDKDTIEKVVKQIEKKIDVIQAHYFTEDEIYFHEIALYKVSTPAFQETP
                     EASKVIRRYNARIVEVNPVFSIVEKNGMSEEITSLYEELRALKCVLQFVRSGRVAITTS
                     CFERVNEFLDGQEAKYKQSKKEQE"
     CDS             2520..3263
                     /locus_tag="ctg334_4"
                     /transl_table=11
                     /translation="MSEENKIGTYQFVAEPFHVDFNGRLTMGVLGNHLLNCAGFHASDR
                     GFGIATLNEDNYTWVLSRLAIELDEMPYQYEKFSVQTWVENVYRLFTDRNFAVIDKDGK
                     KIGYARSVWAMINLNTRKPADLLALHGGSIVDYICDEPCPIEKPSRIKVTSNQPVATLT
                     AKYSDIDINGHVNSIRYIEHILDLFPIELYQTKRIRRFEMAYVAESYFGNELSFFCDEV
                     SENEFHVEVKKNGSEVVCRSKVIFE"
     CDS             3327..4370
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg334_5"
                     /sec_met_domain="IlvN (E-value: 2e-47, bitscore: 149.6,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 1.1e-40, bitscore: 128.0,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQLNFGGTVENVVIRDEFPLEKAREVLKNETIAVIGYGVQGPGQ
                     ALNLRDNGFNVIVGQRQGKTYDKAVADGWVPGETLFGIEEACEKGTIIMCLLSDAAVMS
                     VWPTIKPYLTAGKALYFSHGFAITWSDRTGVVPPADIDVIMVAPKGSGTSLRTMFLEGR
                     GLNSSYAIYQDATGNAMDRTIALGIGIGSGYLFETTFIREATSDLTGERGSLMGAIQGL
                     LLAQYEVLRENGHTPSEAFNETVEELTQSLMPLFAKNGMDWMYANCSTTAQRGALDWMG
                     PFHDAIKPVVEKLYHSVKTGNEAQISIDSNSKPDYREKLEEELKALRESEMWQTAVTVR
                     KLRPENN"
     CDS             complement(4903..5526)
                     /locus_tag="ctg334_6"
                     /transl_table=11
                     /translation="AVSLLKVQYRMHEAIMRFPSEWFYNGELEAAPEVRNRGILDFDTP
                     MNWIDTSEMDFHEEFVGESFGRINKQEANLLLQELEAYISRIGKARILDESIDFGLISP
                     YKAQVQYLRSKIRGSSFLRPFRSLITVNTVDGFQGQERDVVFISLVRANEDGQIGFLND
                     LRRMNVAITRARMKLVILGDATTLTKHAFYRKLIQYIRQEAVSS"
ORIGIN
        1 aaaaatagta gatggagata tcatcgaaat cgacattccc gcccgaacga ttaacgtgcg
       61 actaacagat gaagaactgg ctgcccgccc gatgactccc gtcacccgtg accgttacgt
      121 gccgaagagc ctgaaagcat acgccagcat ggtaagctct gccgataagg gagcagtgag
      181 attaatagac tgatccactt cattatccat tatgaatcat ccattatcca ttaaagaact
      241 atgagtaagg acttaataac aggcgcagag gcaatgatgc gctctctgga acatcaggga
      301 gtgactacca ttttcggtta tcccggcggt tccatcatgc ctacgttcga tgccctgtac
      361 gaccatcaaa atacattgaa ccatatcttg gttcgccatg aacagggagc tgcccatgcg
      421 gcacaaggct acgcccgtgt atcgggtaaa gtcggtgttt gcttggtgac aagcggcccc
      481 ggagcaacca atacgatcac aggtattgca gatgcgatga tagacagtac gcctatcgtt
      541 gtcattgcag gacaggtggg aacaggcttt cttggaacag acgcttttca ggaagtcgat
      601 ctcgtaggta tcactcagcc aatcgctaaa tggagctatc agattcgccg tgccgaagac
      661 gtagcctggg cgatagcacg tgctttctac attgccagta gtggtcgtcc cggtccggtg
      721 gtactcgact ttgccaagaa tgcgcaggtt gaaaaaacaa aatacgagcc gacgcagcag
      781 gagtttatcc gcagctatgt tccggttccc gatacagacg aagaatcagt aaaggctgcc
      841 gccgaactga tcaataacgc cgaacgcccg cttgtcttgg taggacaggg agtggaactg
      901 ggaagcgctc aggaagaact gcgtatcttt atcgaaaagg cggatatgcc cgcaggttgt
      961 accttgctcg gactttccgc attgcctacc gaccatccgt tgaataaagg aatgctgggt
     1021 atgcacggaa atctgggacc gaatatcaat actaacaaat gtgatgtact gattgctgtc
     1081 ggtatgcgtt tcgacgaccg tgtgaccggt aatctggcta cttacgccaa acaagcgaaa
     1141 gtgatccatt ttgacattga tccggcggaa gtaaataaga atgtaaaggt agacattgcc
     1201 gttttgggcg actgcaaaaa gacgctggct gctgtgaccg gactgctgaa aaagaaccgg
     1261 cacaccgagt gggtcgatag tttcaaggaa tatgaagcgg tagaggaaga aaaagtcatt
     1321 cgtcccgaac ttcatcctgc aacggattca ctgagtatgg gcgaagtggt gcgtgcggta
     1381 agcgaagcga cccgtcacga agccatattg gtgacagatg taggtcagaa tcagatgatc
     1441 tctgcccgct actttaaata taccagggaa cgtagtatcg ttacttcggg tggacttggc
     1501 acgatgggtt ttggccttcc ggcagctatc ggagctactt tcggccgtcc cgaccgcact
     1561 gtatgtgtat ttatgggtga cggcggactg caaatgaaca ttcaggaact gggtacgatc
     1621 atggaacaga aagctccggt gaagattatc tgcctgaaca acaattatct cggcaacgtg
     1681 cgccaatggc aggctatgtt ctttaatcgc cgttattcat ttactccgat gctgaatccg
     1741 gattacatga aaatcgcttc cgcctacgat attccttcca aacgtgtctt ttcacgggaa
     1801 gaattgaaag cggccatcga cgaaatgctc tcgacagacg gggcgttcct gctcgaagcc
     1861 tgtgtggtag aagaaggcaa tgtcctcccg atgacccctc cgggcggttc agtcaatcag
     1921 atgttgctgg aatgttaatc cttaatttag aaagttatga gtgataagac attatatacg
     1981 attatagttc attctgaaaa tatagccgga ttgctgaatc aggtgaccgc tgtatttacc
     2041 cgtcggcaaa taaacatcga gagtttgaac gtttctgctt cttccattaa aggagtacat
     2101 aaatatacga ttacagcgtg gactgataag gacactatcg aaaaggtggt gaagcagatc
     2161 gaaaagaaga ttgatgtgat tcaggcgcac tattttacgg aagacgagat ttatttccat
     2221 gagatagcct tatataaggt atctactcct gcttttcagg agactccgga agcatctaaa
     2281 gtgatccgca gatataatgc ccgcattgta gaggtgaatc cggtattctc tatcgtcgag
     2341 aagaacggca tgagtgaaga gattacttcc ctttacgaag aacttcgggc tttgaaatgt
     2401 gtgctgcaat ttgtccgttc gggccgtgtg gctattacta ccagctgttt cgaacgtgtg
     2461 aatgagttcc tggacggaca ggaagcgaag tataagcaaa gtaaaaagga acaggagtaa
     2521 tgagtgaaga aaataaaata gggacttatc agtttgtggc agagccgttt cacgtagatt
     2581 ttaacggccg cctgaccatg ggcgtgctgg ggaatcatct gctgaattgc gcaggttttc
     2641 atgccagcga ccgtggattt ggtattgcta cgctgaacga ggacaattac acatgggtgc
     2701 tttcccgtct ggcgatcgag ctggacgaga tgccttacca atacgagaag ttctcggttc
     2761 agacatgggt ggagaatgtc tatcgtctct ttaccgaccg taactttgcc gtcattgaca
     2821 aggatggaaa gaagatcggc tatgcccgtt ccgtatgggc gatgatcaat ctgaatactc
     2881 gtaagcctgc cgatctgttg gcattgcatg gaggaagcat cgtcgattat atatgcgatg
     2941 aaccctgccc gatagaaaag ccttcgcgta tcaaggtgac aagcaatcag cctgttgcga
     3001 cgctgacggc aaaatacagt gatatcgata ttaacggaca cgtaaacagt attcgttata
     3061 tcgagcacat acttgacttg tttccgatag aactgtatca gacgaaacgt atccgccggt
     3121 ttgaaatggc gtatgtggct gaaagttatt tcggaaatga actctctttc ttctgtgatg
     3181 aggtgagtga aaacgagttt catgtagagg tgaagaaaaa cggcagcgag gtagtatgcc
     3241 gttccaaagt gatatttgaa taatatagag tataaaatca attaataaca gatggcgcaa
     3301 gtcatcaaaa caaaaaaaca aaaaaaatgg cacagttgaa ttttggcgga actgtagaaa
     3361 atgtagttat ccgtgatgaa tttccattgg aaaaagctcg tgaagtattg aaaaatgaaa
     3421 caatcgctgt aatcggttat ggcgtacaag gtcccggaca ggctctgaac cttcgtgata
     3481 acggtttcaa tgtaatcgtt ggtcaacgcc agggaaagac atatgacaaa gcggttgctg
     3541 acggatgggt tccgggtgaa actttgttcg gtattgaaga agcttgcgaa aaaggtacga
     3601 tcattatgtg cctgttgtct gatgcagcag taatgtctgt atggcctact atcaagcctt
     3661 acctgactgc aggaaaagct ctttatttct ctcatggttt tgctattaca tggagtgacc
     3721 gcacaggtgt agttcctcct gcagatatcg acgtaatcat ggttgctcct aaaggttcgg
     3781 gtacatcctt gcgtactatg ttccttgaag gtcgcgggtt gaactcttca tacgctatct
     3841 atcaggatgc aacaggcaac gctatggaca gaacaatcgc attgggtatc ggtatcggtt
     3901 caggttattt gttcgaaaca actttcatcc gcgaagctac ttccgacctg acaggcgaac
     3961 gtggttcatt gatgggagct atccagggtc tgttgctggc acaatacgaa gtgttacgtg
     4021 aaaacggtca cactccttcc gaagcattca acgaaactgt agaagagctg actcagtcat
     4081 tgatgccgtt gtttgcaaag aacggtatgg actggatgta tgctaactgc tctactacag
     4141 ctcaacgtgg tgctctcgac tggatgggcc ccttccacga tgctatcaaa ccggtcgttg
     4201 aaaagttgta tcacagtgtg aagactggta acgaagcaca gatttcaatc gactctaact
     4261 ccaaaccgga ttatcgtgag aaactggaag aagaactgaa agcattgcgc gaaagcgaaa
     4321 tgtggcagac tgccgtgaca gttcgtaaac ttcgtccgga aaataattaa tcagacttat
     4381 actatataag tttttcaaaa ggcgggaaaa tcatcttttc ccgcctcttt tatgtgtatg
     4441 ccactctttt gaatgacaga ataaggatga aagtagcaaa aaagattaaa agatactgat
     4501 aatataggtg aaaaagagca tttatgatta cctttacggc aacaaataaa acagaatagg
     4561 caatgaagaa actttttact tacagaaaat cgatacgtat gaagatgatt tcttctttaa
     4621 tgaatagtat atcagaatgt tacattaaaa gatatttaag ttattctcta ccgaatggta
     4681 gctgtaatac catttcaagt cctttttata tttcgtgttc cattagtgat tcgttgatat
     4741 gatgcagaat tttattctgt agcaggaaaa agtatgcgtt aaattgaatt taagacagag
     4801 cttttggaac agaatttagt gggaaaataa cattgtttgt tttgtttgta aatgggagtc
     4861 ttctttcgtg agaaaggggg cttctttttt tatcccgatg attcaggagg ataccgcttc
     4921 ctgccggata tactgtatca gtttccggta gaacgcatgc ttggtaagag tggttgcatc
     4981 tcccaaaatc acaagtttca tccgggcgcg ggtaatggct acgttcattc gtcttaaatc
     5041 attcagaaaa ccgatctgtc cgtcctcgtt cgcccggacg aggctgataa agacgacatc
     5101 ccgttcctgt ccctgaaagc cgtctaccgt gttgaccgtg atgaggctgc ggaaagggcg
     5161 gagaaaactg cttcctctga ttttacttct caggtattgc acttgcgcct tgtagggcga
     5221 aatcagacca aagtcgatgc tctcgtccag gatgcgtgct ttgccgatcc ggctgatata
     5281 ggcttccagt tcttgcagta acaagttggc ttcctgcttg ttgatgcgcc cgaagctctc
     5341 gcctacaaac tcttcgtgga agtccatttc cgaggtatct atccagttca tgggagtgtc
     5401 gaagtcaagg ataccccggt tgcgcacttc cggtgcggct tccagttctc cgttgtagaa
     5461 ccattcggag ggaaagcgca tgattgcctc gtgcatccgg tattgcactt tgagcagcga
     5521 gacggcag
//
