LOCUS       c00342_gnlPro..         3543 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PAPGJCNG_342.
ACCESSION   c00342_gnlPro..
VERSION     c00342_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 02:17:07
            Original ID  :: gnl|Prokka|PAPGJCNG_342
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3543
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3543
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:2564]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..2564
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3543
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3543
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..323)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.3;
                     E-value: 5.5e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg342_1"
                     /sec_met_domain="Radical_SAM (E-value: 1.5e-23, bitscore:
                     72.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSG"
     CDS             complement(324..2564)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg342_2"
                     /sec_met_domain="Gly_radical (E-value: 6.2e-46, bitscore:
                     143.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.7e-282, bitscore:
                     926.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DVREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     ISALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             complement(2735..3370)
                     /locus_tag="ctg342_3"
                     /transl_table=11
                     /translation="MSHEIRTPLNAIVGFSELMVTCDDPEEKKEYINIIQSNNELLLRL
                     INDILDLSKIESGIIERRKENFNLAKVCNELFVTIQAKMTNPNVELRLDGPNSECWVLL
                     DRNRLKQVWMNFLTNAVKCTKSGYIKMGYGIERKGLRIYVEDTGIGIPDDLHDKVFTRF
                     EKLNEFSQGTGLGLTISRAIVEAAGGEVGFTSKLGVGSTFWAWLPLRP"
     CDS             complement(3401..3541)
                     /locus_tag="ctg342_4"
                     /transl_table=11
                     /translation="TLLVTGIQVERDKEGHVTRYTGITINNTKWEKMIQQLKELKEKAE
                     L"
ORIGIN
        1 aaaccagatg tgtccaaagc agtgtgaaga ccttgctctt ggcacaaacg gaaaaactct
       61 ttcagaaact caggttgcaa taaaggttcc ccaccggtaa cagtcacccc cccacgggcg
      121 ataaaactct tataccgtaa gacttccgtc aatagctcgc cgggagtcat ctgatatttc
      181 accttccctt tcgggttcca agtatccggg ttatggcaat acaggcaacg caacgggcag
      241 ccctgcataa aaacgacaaa gcgaattccg ggaccatcta cggtcccgaa actctctaat
      301 gaatgtatct taccttttat cacttacagt gaatggttaa tggttcttga aataacatcc
      361 aactgttgtt cacgagtcag tttcacgaag tttaccgcat atccggatac acggatcgta
      421 agttgcggat atttctcggg atgttccata gcgtcgatca acaaatcctt atcaaatacg
      481 ttaacattca gatgctgacc tccatccgga gtaaaatagc catccaataa gctaaccaga
      541 ttattgatct gcacttcacg ctcctttccc aacgtagccg gagaaacagc gaaagtataa
      601 gagataccgt cgtgcgcatg ctggaatgga agcttcgcta ccgaagctag cgcagagata
      661 gcacctttcg tatcacgacc gttcataggg ttcgcgcccg gagcgaaagg agttccaccg
      721 atacggccat ccggtgtcgt accagtcttc ttaccatata ccacattaga ggtgatcgta
      781 aggatggact gcgtaggttt cgcatggcga taggtctcgt gctgacgcaa atattccatg
      841 aacttctcgg tgatctctac agcgatacta tccgtacgat cgtcgttatt accgaaagga
      901 acatattcgc cctcacgttc gaaatcaacc gccaagccac gctcgtcacg gatcacacgt
      961 actttcgtat cacggatagc agccaacgaa tcggcaacga tagaaagacc ggcgatacct
     1021 gtcgcacgga tacgctctac atccccatcg tgaagcgaca tctcgaatgc ctcatacgcg
     1081 tatttatcat gcatataatg gattatcttc aaggcgttca catacacttt cgccaaccag
     1141 cgcatcattt gctcatactt agccatcacc tcatcgtatt ccagatactc gccgcgaacc
     1201 ggctcgaaca tcggggctac ctgtacgccg ctgcgctcat cccgtcctcc gttgatcgca
     1261 tataataaac atttcgccaa attcgcacgg gctccgaaga actgcatctg cttaccgatc
     1321 ttcataggag aaacgcaaca agcgatacca taatcatctc cataatccgg acgcatcaaa
     1381 tcgtcgttct catattggat agccgacgta tcgatagaga ccttggcaca gaaacgtttc
     1441 cagttctcgg gagcgttctt aaaccacaga accgtcaaat tgggttctgg tgccggacct
     1501 agattatata atgtatggag ataacgatag gaagttcttg ttaccaaaga acggccatcc
     1561 actccttggc cgcccaagga ctctgttacc caaaccggat caccggagaa caaatcattg
     1621 tattccggcg tacgtaagaa acgaacgata cgcaacttga tgatcagctg gtctaccaat
     1681 tcttgcgcct cctcttcggt caacttaccc tcacggacgt ctttctcgat ataaatatcc
     1741 aaaaacgtag aagtacgtcc gatagacatc gccgcgccat cttggtcttt taccgccgcc
     1801 agataagcta agtacacaaa ctgaaccgcc tcacgagcgt tttgtgccgg acgagtcaca
     1861 tcaaagccat agctagcgca cattttctcg aacgctttca aagccttgat ctgttcggaa
     1921 atctcctcac ggctctggat gatctcttcc gtgatctctt ggatatccaa acgattcaag
     1981 aaacgctttt tctcctcgat caagattgcc gtaccatata aagcgacacg acgatagtca
     2041 ccaataatac gtcctctacc atacgcgtcg ggcagaccgg tgataatagc cgaacgacgt
     2101 gcggcaatca tctcttccgt atatgcggag aaaacgccct cgttatgagt tttacggtac
     2161 ttcgtgaaga tctctttcgt catcgggtcc aaatgataac cataagcttc caagctattc
     2221 tcaaccatac ggataccgcc cttcgggaag ataccacgtt tcagcggagc atcggtttgt
     2281 aacccaacga tcacctcatt ctccttatca atatagcccg ggccatatac atcgatccct
     2341 tgaggtaatt ttgtctcggc gtcataaact cccttttccc tctcgacctt aaacatctcg
     2401 gtcagcttgt tccagacctt acgggtcttc tcggaagaag cgactaaaaa ggaatcatcc
     2461 ccttcataag gcgtatagtt actctggata aaatctctta cattgatctc tcttttccag
     2521 gtctcaccct taaagtgatc ccagcactca ttctcaaatt tcataaacaa tcttatctaa
     2581 taccacattt ttaccgccgc aaaggtagga gaattatcat catatttctg tcacatatgc
     2641 tacaccagtt ctaaaatccc tataaattgc gataaaaaag cttctcatcc ttcactattt
     2701 taaaaggaca ttaccaataa atggggagtc agacctaagg gcgaagaggc aaccatgccc
     2761 aaaaagtaga tccgacacca agttttgaag taaagcccac ttctccccct gccgcttcta
     2821 cgatagctct ggagatcgtc aagccaagtc cggtaccctg cgagaactca ttcaatttct
     2881 caaaacgggt aaatactttg tcatgcaaat catccggtat accgattccc gtatcctcaa
     2941 catagatacg aagacctttc ctttcaatgc catatcccat tttaatatat ccggatttgg
     3001 tgcattttac cgcattagtg aggaaattca tccatacttg cttaagccgg tttctatcca
     3061 gaagtaccca gcactccgag ttgggaccat ctaaacgtaa ctcgacattc ggatttgtca
     3121 tcttcgcctg aatcgtaaca aataactcat tacaaacctt agccagattg aaattctcct
     3181 tcctacgttc aattatgcct gactctatct tggaaagatc aaggatatcg ttgatcaatc
     3241 gcaataacaa ctcattattg gattggatga tatttatata ttcttttttt tcttccggat
     3301 catcacaagt taccattaac tctgaaaaac caacaatagc gtttaacggg gtacgtatct
     3361 cgtgactcat attagcgaga aacgcagact tgagacgatc ctaaagttcc gctttctcct
     3421 ttaattcctt caattgctga atcatcttct cccacttcgt attattgatc gtaataccgg
     3481 tatagcgagt cacatggcct tctttatctc tttcaacctg gattccagta accaataagg
     3541 tct
//
