LOCUS       c00021_gnlPro..         6498 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EMCJDHFM_21.
ACCESSION   c00021_gnlPro..
VERSION     c00021_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 05:07:58
            Original ID  :: gnl|Prokka|EMCJDHFM_21
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6498
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6498
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2809:6299]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2810..6299
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..6498
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6498
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             116..823
                     /locus_tag="ctg21_1"
                     /transl_table=11
                     /translation="MNKLLSTLILSAGMSLSATAATSLLDSSIVGYSDFASQTADFGTL
                     KDSVSITSDDSNLTLSGITFNGYGANNRDVMSVTMVLDLSKINTPEAYTALFNAKNSSV
                     SWGVGLNTDRKLQGLWNNTSYNGPVVDTPLGTEGSLTISVVTGDLGTRIYFGDSATYYS
                     NSGLKFGNQQIDQILMDAGLAESIEQLYIHDSLLSQEQVGQLMSEIASIPEPATASLGL
                     LGLAALMMRRRRA"
     CDS             1252..1485
                     /locus_tag="ctg21_2"
                     /transl_table=11
                     /translation="MNLQKQQLLLRLFRYFKSRKGEPRFFNKRNICLLAAAGAYILTPD
                     EFLFDGIGRLGLLDDAAVILGAVLTVFFPDGD"
     CDS             complement(1519..2304)
                     /locus_tag="ctg21_3"
                     /transl_table=11
                     /translation="MVCRTTDEYRAMAQAGCLAVGEPAFWAGYDRSSADGFRDYFVQLT
                     ECEPARAAKFGMDHYTWLCINPKEAQDVAFARDVMKLIPEFIDRPNVLGIGEIGLNRNT
                     SHEMTIFEEHLDLAVRLNQLVLIHTPHLEDKLKGTVMILSALKNRPDLDPCRVLVDHCE
                     EHTFPLVKEAGYWAGLTLYPTSKLNPKRAADILELYGMDRVWANSAADWGDSDPLALTA
                     LACELRRRGFSRQETERLLLENPETFMGQSPKFRKVSSR"
     CDS             complement(2337..2609)
                     /locus_tag="ctg21_4"
                     /transl_table=11
                     /translation="MKRSKKEVLDADFAAARAGVLDVAAFLDRVDRGEGEADFRYHALM
                     DVLPLLSASGPGRVRAILEALSYRDDTVPETAAAKAACGAPNLSK"
     CDS             2810..3511
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg21_5"
                     /sec_met_domain="SucD_FumR (E-value: 2.9e-14, bitscore:
                     42.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNALVKTICTFVTSSIGRKIIVALTGLCLVLFLAGHLAGNLLIFG
                     GPEWINTYAHGLHSMPEAALWGIRAGLAVIFIIHVWLTIQLKLENHAAREPYVFKNTIK
                     ATLSSRYMIYTGLTVLVFLIYHLYQYTLRVGYDPAQFTTFISDGTVETFDVYKMIVTGF
                     SNVWCSAFYILAVLMLFSHLRHGVQSIFQTVGVDSRKIRPFYNFIAIAYGAIICLGFIS
                     VPVSVLLGIIK"
     CDS             3533..5491
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 176.1; E-value:
                     4.2e-54)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg21_6"
                     /sec_met_domain="FAD_binding_2 (E-value: 4.7e-55, bitscore:
                     176.1, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 1.3e-38,
                     bitscore: 120.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINFPVSDWMPDAKIPSGPIQDKWSKYKLESKLINPNNKRKYTVL
                     VVGSGLAGASAAATLAELGYNVKCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRL
                     FYDTVKGGDFRAREANVYRLAEVSSNIIDQCVAQGVPFGREYGGLLDNRSFGGAQLKRT
                     FYARGQTGQQLLLGAYQALEKEVAKGHIEMFSRHEMMDLVLVDGEAKGIVTRNLVTGEI
                     KSYAGDTVLLCTGGYGNVFFLSTNAMGCNVTAAYKAYKRGAFFANPCFTQIHPTCIPVK
                     GDYQSKLTLMSESLRNSGRIWVPKSRETAEKIRKKEIKPSDVPEEERDYYLERKYPSFG
                     NLAPRDISSRAAKEACDDGRGVAVTGKGVFLDFADEIKRMGREWIDGQYGNLFDMYEEI
                     TDDDPHETPMMIYPASHYTMGGLWVDYNLMSNVKGLHVLGEANFSDHGANRLGASALMQ
                     GLSDGYFVIPATLPAYLTTTKPGAVTTAAPEFKAAEDEVKERINKLLNVKGTKTVDSFH
                     RELGLIMWEDCGMARTEESLTHAIERIPQLRKEFWENVRVTGTADGINAELEKACRVAD
                     FLEFAQLLCYDALNRQESCGAHFRVEYQLPDGEAKRNDDEYAYVAAWEYTGVDSLPVLH
                     KEPLTFENVHLAIRSYK"
     CDS             5532..6299
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Succinate dehydrogenase-2Fe-2S iron-sulfur
                     cluster binding domain (Score: -1.5; E-value: 8.6)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg21_7"
                     /sec_met_domain="Fer2_3 (E-value: 2.5e-24, bitscore: 74.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 8.4e-12, bitscore: 34.5,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 4.8e-07, bitscore: 19.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTLNLTLKVWRQENREAKGRIETYAAKDIPVDASFLEMLDIVN
                     EGLVKEGKEPIHFDHDCREGICGMCSLTINGIPHGPERQVTTCQLHMRKFKDGDTVWIE
                     PFRAKAFPILRDLMVDRSALDRIIAAGGYIDVRTGSAPNANNIAVEKVKADAAFDAAAC
                     IGCGACVASCKNASAMLFTSAKIAHLNLLPQGQPERSKRVLAMMKQMEAEGFGNCTNQY
                     ECEAACPKGISVDNIARLNRDFMRANAKEGLCF"
ORIGIN
        1 ttataaacac aatatactaa ctcttaatat ttttaattgc ttgcggattc caaattcgtt
       61 attcaagcac aagtacatac attgaacctt gaatcatttt attaagatac tgaccatgaa
      121 taaattatta tccaccctga ttctttcggc cggcatgagc ctgagcgcta ccgcggctac
      181 ctctctgctg gatagctcca tcgtgggata ttcggacttt gcctcccaaa cggcggattt
      241 cggcaccctg aaagattccg tttccatcac gtctgacgac agcaatctga cgttatccgg
      301 gataaccttc aacggatacg gtgccaacaa ccgcgacgtt atgtctgtga cgatggttct
      361 tgacctgtcc aaaatcaaca ctccggaggc ctataccgct ctgtttaacg ccaaaaacag
      421 cagtgtaagc tggggcgtgg gcctgaacac ggaccggaaa ctccagggat tatggaacaa
      481 cacctcatac aacgggcccg tcgttgacac cccgctgggc acggaaggtt ccctgaccat
      541 ttccgtagtc accggagatc ttggcacccg catctatttt ggcgacagcg ctacgtacta
      601 ttccaacagc ggcctgaaat tcggcaacca gcaaattgat cagattctca tggacgcagg
      661 actggctgaa tccatcgaac agctctacat ccacgactcc ctcctttccc aggaacaagt
      721 tggtcagctg atgagtgaaa tcgccagcat tccggaaccc gccacggctt ccctgggcct
      781 gctgggactg gccgcgctga tgatgcgccg ccgcagggca taagcctgta ccgtccgcct
      841 ccctatattc aaaggccggc tcacaaaatc gtgagccggc cttttcctta tccgttcaaa
      901 catctgccga aaaaacagag gggaaaagct tccatttgct gcggcaacct cacggccagc
      961 acttgacgga aaaatggcat tcagtctccc ccggtagggt catttcaaga gcttccggcc
     1021 ccgccccact tccgtacacg cccctttttc ccatgccggg acagccggaa ccggagcatg
     1081 caaagcccgg cttccctcag catccggaac agcccatcct ttcagcaatc catttccccg
     1141 cacggtccgg atcgctgcgg ttccgtccgt cggcaacaga ctccttcccc gaaacaggga
     1201 aaaggagtgg gaaaatggct gagcttgaac ctttatcccc cgttcccgtc catgaacctt
     1261 caaaaacagc agctgctact gcgcctgttc cggtatttca aaagcagaaa aggagaaccg
     1321 cgttttttca acaaacggaa tatatgcctt ctggccgccg ccggcgccta catcctgact
     1381 cccgacgaat tcctgtttga cggaatcggc aggctgggcc ttctggatga cgcggcggtc
     1441 atcctgggcg ccgtcctcac cgtctttttc ccggacgggg actagagggc cgtaccctcc
     1501 gccgcgcctc gggcggaatt agcgggacga cactttccgg aacttcgggg actgtcccat
     1561 gaatgtctcc ggattttcca ggagaagacg ttccgtttcc tgccgggaaa aaccgcgcct
     1621 gcgtagctcg caggccaggg ccgtcagcgc caggggatcg gaatcccccc agtccgccgc
     1681 ggaattggcc cagacacggt ccattccgta caattccaga atatccgccg cccgcttggg
     1741 attcagcttg ctggtgggat aaagggtcag cccggcccag taaccggcct ccttcaccag
     1801 cgggaaggta tgttcctcac agtgatccac cagcacgcgg caagggtcca gatccggacg
     1861 gtttttcagg gcggaaagaa tcattacggt ccccttaagc ttgtcttcca gatggggagt
     1921 gtgaatcagc acaagctggt tgaggcgcac cgccagatcc agatgctctt caaaaatagt
     1981 catttcatgg gaagtattcc ggttcagccc tatttccccg atgccgagga cattgggccg
     2041 gtcaatgaac tccggaatca gtttcatcac atcccgcgcg aaagccacgt cctgcgcctc
     2101 cttgggattg atgcacagcc atgtgtaatg gtccatgccg aacttggccg ccctggcggg
     2161 ttcgcattcc gtaagctgta cgaaataatc ccggaaaccg tccgcggacg aacggtcgta
     2221 accggcccag aacgccggtt cccctaccgc caggcagccg gcctgcgcca tggcccggta
     2281 ttcgtccgtg gtgcggcaca ccatatgtat atgaggttct atgaaagcca tcattcttat
     2341 ttgctgaggt tcggggctcc gcaggccgct ttggctgcgg cggtttccgg cacggtgtca
     2401 tccctgtagg aaagggcttc cagaatggcc cggacgcgtc cggggccgga tgcggacaag
     2461 aggggaagaa catccatcag ggcatggtag cggaaatccg cctccccttc cccgcggtcc
     2521 acccggtcca gaaatgcggc cacatccaga acgccggcgc gcgccgctgc gaaatccgcg
     2581 tccagcacct cctttttact tcttttcatg cacagacgat acgcctatgc ggaaagcctc
     2641 gtcaagccgg aacaggcaac aaaatgataa ccattccaaa ctgataaaca gcaacctctt
     2701 tccccggttt ttcaaaaatc cgaaaagcgc cggaaattca agaagaggct tgccatccgg
     2761 gcttgaaccg ggcatcctgt gaagacgcat ttatttcatt agagcgccta tgaatgcact
     2821 agtaaaaacc atatgtacat tcgtgacatc ctccatcggc cgcaaaatca ttgtggctct
     2881 gacggggtta tgtttggtct tgttccttgc gggacacctg gcaggcaacc ttctcatctt
     2941 cggaggccct gaatggatca acacctacgc ccacggcctg cactccatgc cggaagcagc
     3001 cctctgggga atccgcgcgg ggcttgcggt gatcttcatc atccacgtct ggctgaccat
     3061 ccagctgaag ctggaaaacc atgcggcccg cgaaccctac gtgttcaaga acaccattaa
     3121 ggcaacgctc tcctcccgct acatgatcta taccggtttg accgtcctgg tgttcctgat
     3181 ctaccacctg taccagtaca ccctgcgcgt gggatacgac cccgcccagt tcaccacctt
     3241 catttccgac ggtacggtgg aaacctttga cgtgtacaag atgatcgtga ccggattctc
     3301 caacgtatgg tgctccgcct tctacatcct ggccgttctg atgctgttca gccatctgcg
     3361 ccacggggta cagtccatct tccagactgt gggcgtcgat tcccgcaaaa tccggccctt
     3421 ctacaacttt atcgccatcg cctacggcgc gattatctgc ctgggcttca tctccgtgcc
     3481 ggtctcggtt cttctgggta tcatcaaata accatcaact cctctcaaag tcatgattaa
     3541 ttttcccgta agcgactgga tgccggatgc caaaattccc tccggcccta ttcaggacaa
     3601 atggtccaag tacaaacttg agtccaaact gatcaatccg aacaacaagc gcaagtacac
     3661 ggtgcttgtg gtaggttccg gcctggctgg cgcctccgct gccgccacgc ttgccgaact
     3721 tggctacaac gtcaaatgct tctgctacca ggacagcccc cgccgcgccc actccattgc
     3781 ggcccagggc ggcattaacg cagccaagaa ctaccagaac gacggcgact ccgtttaccg
     3841 cctgttttac gacaccgtta aaggcggcga cttccgcgcc cgtgaagcca atgtctaccg
     3901 tctggcggaa gtctcctcca acatcattga ccagtgtgtg gcccagggcg ttcccttcgg
     3961 ccgtgaatac ggcggcctgc tggacaaccg ctccttcggc ggcgcccagc tcaagcgaac
     4021 cttctacgca cgcggacaga cgggccagca gctcctgctg ggtgcctacc aggccctgga
     4081 aaaggaagtg gccaagggac acattgaaat gttctcccgc catgaaatga tggatctggt
     4141 gctggtggac ggcgaagcca agggcatcgt cacccgcaat ctggttaccg gcgaaatcaa
     4201 atcctacgca ggggataccg tcctgctctg cacgggcggc tacggcaacg tgttcttcct
     4261 gtccaccaac gcgatgggct gcaacgtcac tgccgcctac aaggcctaca agcgcggtgc
     4321 cttcttcgcc aacccctgct tcacgcaaat ccaccccacc tgcattccgg tcaagggcga
     4381 ctaccagtcc aaactgacgc tgatgtcgga atccctccgc aacagtggcc gcatctgggt
     4441 gcccaagtcc cgtgaaaccg cagaaaaaat ccgtaaaaag gaaatcaagc cttccgatgt
     4501 tcccgaagaa gaacgggact actacctgga acgcaagtat ccctccttcg gcaacctggc
     4561 tccgcgcgac atttcctccc gcgccgccaa ggaagcctgc gacgacggcc gcggcgtggc
     4621 cgtcaccggc aagggcgtct tcctggattt tgcggatgaa atcaagcgca tgggccgcga
     4681 atggattgac ggccaatacg gcaatctgtt tgacatgtac gaagaaatca cggacgacga
     4741 cccccatgaa acgcccatga tgatctaccc cgcctcccac tacaccatgg gcggcctgtg
     4801 ggtggactac aacctcatgt ccaacgtgaa ggggctgcac gtcctcggtg aagccaactt
     4861 ctccgaccac ggcgccaacc gcctgggagc ctccgccctg atgcagggcc tgtctgacgg
     4921 ctacttcgtc attcccgcca cgctgcccgc ttacctgacg accaccaagc ccggcgccgt
     4981 caccaccgcc gccccggaat tcaaggctgc ggaagacgaa gtgaaggaac gcatcaacaa
     5041 gctcctgaat gtgaagggca ccaagacggt ggactccttc caccgggaac tgggcctcat
     5101 catgtgggaa gactgcggca tggcccgcac ggaagaaagc ctgacccacg ccattgaacg
     5161 cattccccag ctccgcaagg aattctggga aaacgtgcgc gtaaccggca cggcggacgg
     5221 catcaacgcg gaactggaaa aagcctgccg cgtggccgac ttcctggaat tcgcccagct
     5281 gctctgctat gacgccctga accgccagga atcctgcggc gcccacttcc gcgtggaata
     5341 ccagctgccg gacggcgaag ctaagcgcaa cgacgatgaa tacgcctatg tagccgcctg
     5401 ggaatacact ggcgtggaca gcctgcctgt tctccacaag gaaccgctga ccttcgagaa
     5461 cgtgcacctt gccatccgca gctacaagta aagatagcca atacattaac actcaaaaga
     5521 aagattataa catggctaaa acactcaatc tcactctcaa ggtctggcgc caggaaaacc
     5581 gggaagccaa gggccgcatt gagacctatg ccgccaagga cattccggtg gacgcctcct
     5641 tcctggaaat gctggacatc gtcaatgaag gcctggtcaa ggaaggcaag gaacccatcc
     5701 attttgacca cgactgccgg gaaggcatct gcggcatgtg ctccctgacc atcaacggca
     5761 ttccccacgg gccggaacgc caggtgacca cttgccagct tcacatgcgc aagttcaagg
     5821 atggcgatac cgtctggatt gaacccttcc gcgcgaaggc tttcccaatc ctgcgggacc
     5881 tgatggtgga ccgctccgcc ctggaccgca tcatcgccgc tggaggctat attgacgtgc
     5941 gtaccggttc cgcgccgaac gccaacaaca tcgccgtgga aaaagtgaag gcggacgccg
     6001 cctttgacgc ggccgcctgc atcggctgcg gcgcctgcgt ggcctcctgt aaaaacgctt
     6061 ccgccatgct gttcacctcc gccaagatcg cccatctcaa tctgctgccg cagggccagc
     6121 cggaacgttc caagcgcgtc ctggccatga tgaagcagat ggaagcggaa ggctttggca
     6181 actgcacgaa ccagtatgaa tgtgaagccg cctgcccgaa aggcatcagc gtggacaaca
     6241 tcgcccgcct caaccgcgac ttcatgcggg ccaacgccaa ggaaggcctc tgtttctaaa
     6301 gaagattcct tcttaagacg ctctgtaaaa actcccggcg gcatcggctg tcgggagttt
     6361 tttcattacc agatttcctt gaaccaggaa taaaatacca gacagttgct tcctcatcgt
     6421 cgcgcagcgt aaaaaattgt tacaccggaa ggcacccagt aatccaggag accgtacttg
     6481 gaaatgccgg acgaaacc
//
