LOCUS       c00137_gnlPro..         5155 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LGNFGKJK_137.
ACCESSION   c00137_gnlPro..
VERSION     c00137_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 06:00:26
            Original ID  :: gnl|Prokka|LGNFGKJK_137
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5155
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5155
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[298:3278]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      299..3278
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5155
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5155
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..296
                     /locus_tag="ctg137_1"
                     /transl_table=11
                     /translation="SLYNTFSTSLLLVFSAWLSSCVSQIPLTKEKPVNNSTYQVEYLFE
                     HEGCKVYRFRDMGHYIYFTNCEGDVTSIESDSVRTVNRISRKPVTIREQTLD"
     CDS             complement(299..1021)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73.4;
                     E-value: 7.1e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg137_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.3e-36, bitscore:
                     114.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGRIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWEVKR
                     ETPYLLEPEALLAEVLRYKNFIAKGGVTVTGGEPLLQPEFLKEFFRLCRENGIHTALDT
                     SGYICSGKALEVLEYVDLVLLDIKTIDAGLHPRLTAVKLDNTLRFLDELEKRGIPVWVR
                     HVVVPGLTDNDEDLEKLARYVSRYKVVQKAELLPYHTMGAYKYEAQGLDYKLKGVEPLS
                     AERLTNAKAIFSKHGITV"
     CDS             complement(1038..3278)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg137_3"
                     /sec_met_domain="Gly_radical (E-value: 2.7e-46, bitscore:
                     144.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 6e-283, bitscore:
                     927.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGETWKKEINVRDFIQNNYKPYNGDDTFLVPSSE
                     KTKKVWNKLTEMFKVERKKGVYDAETKLPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTDEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVPLYGTQILIEEKKRFQKRLDIQELTEEIIQSREEITEQIKAL
                     KAFEKMCAGYGFDVTRPAKDAREAVQFLYLAYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DICEGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPDNWKNFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDEKSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMGLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRKATPTQSILTITSNVVYGKKTGTTPDGRPGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQVNNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLLDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINSSL"
     CDS             complement(3705..5153)
                     /locus_tag="ctg137_4"
                     /transl_table=11
                     /translation="IDGNDVNGDVTFGNGVKWATTKIALNPVTVEDKFASFVNREEGAG
                     LVTFALAKNDEAAPEFYVGAKRNAKGLMTGEIFSYSDALDAAQFELEKSEKFLTALNTY
                     MYVKNGRPITSTEKDTVAFYTYKVKAFDADQDNMYLKWGSNNFDLGATGLNFVIKENID
                     GSVSLIDYNTTNKLEGNSLKVADKLKDGKTAPWATASAYDLTTLLSEGLKTYMVEESPL
                     VSLEAVPQHISFEAVRGGFMTMDENKDARLAIANEASEDLTFWVDTVHSNLNIPSFYIT
                     KGGNFLYNAVDSASYYAARKNYRFNLENKATGDAKLIFKAGELVSSDTLRTIVDGKSVL
                     VAEKDNAPKKIKGGLKNFQFQIVRAEEGSDEYVIRKDGQYVCQYNNYFYMKADKSEAYR
                     FVIEKQSAPTANEGIATSEVKVIAGEGNVTIAGAAGKKVVISNILGQVVANTVISSDNA
                     TIAAPAGVVVVAVEGEAAVKAIVK"
ORIGIN
        1 gtagtttgta taatacattt tcaacaagtc tattgctcgt tttcagtgca tggctttctt
       61 catgcgtctc acaaataccc cttacaaagg aaaaaccggt aaacaattcc acttatcaag
      121 tcgaatacct attcgaacac gaagggtgta aggtctatcg tttcagggat atgggacatt
      181 acatctattt caccaactgc gagggagatg tgaccagtat tgaaagcgac agcgtccgca
      241 cagtcaaccg gatcagccgt aaaccagtca ccatcagaga gcagacactc gattaggctt
      301 acacggttat cccgtgttta ctaaagatcg ctttagcatt ggtcagacgt tctgccgaaa
      361 gcggttccac acctttcagc ttataatcca ggccctgcgc ctcatactta taggctccca
      421 ttgtgtgata aggcaaaagc tcggctttct gaaccacttt atagcgactg acgtatctgg
      481 ctaatttttc cagatcctca tcattatccg tcaggccggg aacgaccaca tggcggaccc
      541 atacgggaat gcctcgtttc tccagttcat caagaaaacg caaggtgtta tcaagcttca
      601 cagcagtcag acggggatga agcccggcgt cgatcgtctt gatgtccaac aaaaccaaat
      661 cgacatattc aaggacttcc aatgctttac cggaacagat atagccggaa gtgtccaacg
      721 ccgtatgaat accgttctca cggcaaaggc ggaaaaactc tttaaggaat tcaggttgta
      781 acaaaggctc gccaccggtc acggtaacac cacctttagc gataaaattc ttatagcgta
      841 gcacttcggc aagcaacgct tcaggttcca gcagataagg cgtttcacgc tttacctccc
      901 aagtatccgg attgtggcaa tacagacaac gaagcggaca tccctgcata aaaacgacaa
      961 agcgaattcc gggaccgtca acggtcccga aactctctaa agaatgtata cgccccaaca
     1021 tattaatcaa tggacaatta taaactgctg ttgatggtac gcgagatgac gtcgagctgc
     1081 tgttcgcgtg tcagcttcac gaagttgacg gcatagcccg aaacacggat cgtaagctgc
     1141 gggtatttct ccggatgttc catcgcatct aacagcaagt ctttatcgaa tacgttcaca
     1201 ttcagatgct ggccgccatc gggcgtgaaa taaccatcta agaggctcac taagttgttc
     1261 acctggatat cgcgttcctt acccaacgtt gcaggagaaa cagcgaatgt ataggaaata
     1321 ccgtcatgcg catgctgaaa aggcagtttg gctacagaag ccaaagcggc gacagcccct
     1381 ttcgtatcgc gtccgttcat cgggttagct cccggagcga acggcgtacc gcccggacgg
     1441 ccgtcgggag tcgttccggt tttctttccg taaaccacgt ttgatgtgat cgtcaggata
     1501 gattgtgtcg gagttgcctt gcggtatgtc tgatgctggc gcagatattc catgaacttc
     1561 tcggtaatgt caacagcaat actgtccgtg cggtcgtcat tgtttccgaa aggtacatat
     1621 tcgccttcgc gttcgaagtc gacagccaaa ccacgttcgt cacggatcac tttcactttc
     1681 gtatcacgga tggcagccag cgagtcagca acgatagaaa ggccggcgat accggttgcg
     1741 cggatacgtt ccacatctcc gtcatgaagc cccatctcga atgcttcata cgcatatttg
     1801 tcgtgcatgt agtgaatgat cttcagagca ttgacataaa cttttgccaa ccagcgcatc
     1861 atctgctcgt atttctccat cacttcgttg tagtccagat actcgctggt gatcggttcg
     1921 aaacggggag ccacctgcac acccgatttc tcgtcacgtc cgccattgat cgcatacaac
     1981 aagcacttgg ccaggttggc acgagctccg aaatactgca tctgcttacc gatcttcatc
     2041 ggagacacac agcaggcgat accgtaatcg tcgccatagt ccggacgcat cagatcgtcg
     2101 ttctcgtact ggatggcaga ggtatcgatc gatacttttg cgcagaagtt tttccagtta
     2161 tccggagcat tgttgaacca aagcaccgtc aggttgggtt cgggtgccgg gccgaggtta
     2221 tacaaggtat gcagataacg gtaagatgtc tttgtcacca gcgaacggcc gtcaacacct
     2281 tgtccgccca gcgattcagt cacccaaaca gggtcgccgg agaacaggtc gttgtattcc
     2341 ggagtacgca ggaaacggac aatacgcagt ttgataataa actggtcgat caattcctgt
     2401 gcctcttctt ccgtcagctt gccttcgcaa atatcttttt cgatatagat atcgaggaaa
     2461 gtagaagtac gtccgagaga catggctgct ccatcctgat cctttacggc tgccaggtaa
     2521 gccagataca agaactgcac ggcttcgcgg gcatcctttg ccggacgtgt cacatcgaaa
     2581 ccataaccgg cacacatttt ctcaaatgct ttcagcgctt tgatctgctc cgttatctcc
     2641 tcacgactct gaatgatttc ttctgtcagt tcctgtatat cgagacgttt ctggaaacgt
     2701 ttcttttctt cgatcaggat ctgggttccg tacagaggta cacgacggta atcaccgatg
     2761 atacgtccgc gtccgtaggc atcaggcaac ccggtaatga tggcggaacg acgtgccgca
     2821 atcatctcgt ctgtataggc tgaaaaaacg ccttcgttgt gcgtctttct gtatttggtg
     2881 aagatttctt ttgtcatcgg gtccagatga tagccatagg cttccagact gttttcgacc
     2941 atgcggatac cgcctttcgg gaagataccg cgtttcaacg gggcgtctgt ctgcaagccg
     3001 acgattactt cgttttcctt atcgatgtaa ccgggtccgt atgtgtcaat cccctgggga
     3061 agcttcgttt cggcatcata tactcctttc ttcctttcaa ccttaaacat ctcggttagt
     3121 ttgttccaca ctttcttcgt tttctccgaa gagggtacga ggaacgtatc gtctccgtta
     3181 taaggtttgt aattgttctg gataaagtct cttacattga tctccttttt ccaggtttct
     3241 cctttgaact gcgcccagtt ctcgttttca aacttcataa gcttatcttg tctatttcgg
     3301 tttaggcacg caaagatacg tcttttcccc ctatctgaaa aggattgtaa aggacgaggg
     3361 atgttaaacc ggagaaaata cctacaaata gggataaggg aaataaaaac gaagccccgg
     3421 cagtacgaat accgccgggg cctgtttttt gtctgccgtc cggtcttcac agagggaaaa
     3481 gcagtagaac ttttaatttc attactttag tattattaat attaaatcca accgcttgtt
     3541 cgcctgtcgc agggtaattg aaaccataga tcaagaaaac ctcttacata cggtttcacg
     3601 caggttgtct agaattattt gattataaaa tgtcattgtg cctgtgtaag ggtggttcgc
     3661 taaccaccct tacgatagac agtattgttt tttagtatca atcgttactt aacgatagct
     3721 tttacagcag cttcaccttc aacagctaca actactacac ctgccggagc agcgattgtt
     3781 gcgttgtcag aagagattac tgtgttagct actacctggc ctaagatgtt gctgataaca
     3841 actttcttac cggcagcgcc tgcgattgtt acgttacctt cgccagcgat cactttcact
     3901 tcagatgttg cgatgccttc gttggctgta ggagcagact gtttttcgat gacgaagcga
     3961 taagcttcag acttgtcagc cttcatgtag aagtagttgt tgtactggca tacatactgg
     4021 ccatctttac ggatcacata ttcgtcacta ccttcttcgg cacgtacgat ctggaactgg
     4081 aagttcttca aaccaccctt gatcttcttc ggagcattat ctttttctgc aaccaaaaca
     4141 gacttgccgt caacgatagt acgcaaagta tcggaagaaa ccaattcacc tgctttgaag
     4201 atcaacttgg catcacctgt cgctttgttt tccaagttaa agcgatagtt cttgcgagca
     4261 gcgtagtagc ttgcagagtc tacggcattg tacaagaagt ttccaccctt cgtgatatag
     4321 aatgacggga tgttcaagtt cgaatgaacc gtgtcaaccc agaatgtcaa gtcttcgctt
     4381 gcttcattgg caatagccag gcgagcatct ttgttttcat ccattgtcat gaaacctcca
     4441 cgaacagctt cgaaagatat atgctgcgga acagcttcca aacttactaa cggagattct
     4501 tctaccatgt aagtcttcag accttcgcta agcagagttg tcaagtcgta agccgaagca
     4561 gtagcccaag gagcagtctt gccatctttc aatttatcgg caaccttcaa gctattacct
     4621 tcgagtttat tagtagtgtt ataatcaatc aagctaacag aaccatcaat attttctttg
     4681 ataacgaaat tcaaaccagt tgcacctaaa tcgaagttgt tagaacccca tttcaaatac
     4741 atattgtctt gatcagcatc aaaggcttta accttatatg tatagaaagc aaccgtgtcc
     4801 ttttctgtag aagtgatagg acggccgttc ttcacataca tatatgtatt cagagcagtc
     4861 aagaacttct cgctcttttc caattcgaac tgagcagcgt ccaaagcatc actataagag
     4921 aatatttcac cggtcatcaa acctttagca tttctcttgg cacccacata gaattcagga
     4981 gcagcttcat cgttcttagc caaggcgaat gtcaccagac ctgcaccttc ttcacggttt
     5041 acgaaagaag caaacttgtc ttctacagtt accggattca acgcaatctt ggttgtagcc
     5101 catttaacac cattaccgaa tgtaacgtct ccgtttacat cattaccatc aatat
//
