LOCUS       c00230_gnlPro..         7414 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JCOLNFCF_230.
ACCESSION   c00230_gnlPro..
VERSION     c00230_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 02:35:13
            Original ID  :: gnl|Prokka|JCOLNFCF_230
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7414
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7414
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[5130:7413]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      5131..7413
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7414
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7414
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             41..175
                     /locus_tag="ctg230_1"
                     /transl_table=11
                     /translation="MENSCNDELFCSLKNELREVTAQINVHSEKLMELLDQKKESSVN"
     CDS             184..429
                     /locus_tag="ctg230_2"
                     /transl_table=11
                     /translation="MSHLTPVTIEYRGNPKQYVSVVLDAINRGRLTYDGIANCEQTFRA
                     LASVVDVISPKNGKTLSVETLVSYEKKKRAGEFEEK"
     CDS             486..818
                     /locus_tag="ctg230_3"
                     /transl_table=11
                     /translation="MKREIIAYKGYFKEFFDRLDTGTQDKILYVLMLLQTQDRIPLKFM
                     KLIEEGLYELRIEYQSNIYRIFFCFDEGRIVILFNGFQKKTEKTPKKEIEKAKILRKEY
                     YGSKNK"
     CDS             799..1104
                     /locus_tag="ctg230_4"
                     /transl_table=11
                     /translation="MEAKTNEEFFNVSALIDERFGKEGTVTRAEAEEKAYAFYTGQIIE
                     DARKKAKITQAELARRIGSDRSYISRVESGQTEPKVSTFYRIMNALGCRIEFSMSL"
     CDS             1122..1211
                     /locus_tag="ctg230_5"
                     /transl_table=11
                     /translation="MVGNLKKLTVFFKFPTVFLRKKEEEKGKR"
     CDS             complement(1353..3470)
                     /locus_tag="ctg230_6"
                     /transl_table=11
                     /translation="MDHFTIFQDFYRVIAEMTEEEIVSAISNGTYRETVEDVRRIFAEQ
                     GEKAANEKKIELPEITFSANYRGGRSNATLVKYLGYIVVDIDHQTQEALARILALAKKC
                     AHTRIAFISPKGMGLKIVVRVCRTDGTLPETIQEIEDFHHAAYHKIASFYAQLCGVEVD
                     TSGQDVSRTCLFSYDPDIYFNPDATAVIVEQPPMFFKSQKKKRASGKKKKTTAPDNNPL
                     TEQVALNYHSSHTSLMVTLNYYHNKSEKYVTGNRNNYLHCLACMYNRYGVPQEEAAAFI
                     KSQFTDLPADEMDALIGSAYGHNEEFDTRKLNSTQKRMLQIEQHIKENYDTRYNEVLHI
                     MEYRRRKTDTEQPEPFHILDEMMENSIWMEMNELGYSCTVKTIQNLIYSDFSITYHPIR
                     EYLDSLPEWDGTDYIGILANSVHTSHQKFWVECLERYLVGMCAAATQDDVVNHTVLLLC
                     SEIQNIGKTTFINNLLPPELRAYLSTGLINPSNKDDLAKIAQAMLINLDEFEGMSGREL
                     NIFKDLVTRKVISIRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHVDSME
                     FIKINYAQLYAQIKYLLNKPGYQYWFTQSENSRIEENNEDFIFHSPEEELVLTHIRKPE
                     RFEKVHYLTVTEIAELIRERTGYQYSHGTKAQLGKVMSKHGFEFHKGKNGRRYTVFIID
                     TEQVKSNRLYE"
     CDS             complement(3558..3788)
                     /locus_tag="ctg230_7"
                     /transl_table=11
                     /translation="MYYPDQPFHIRTYKKSELAHLYNPNVCLKVALQILRRWIVYNPPL
                     LQELEQEGYRARNRLLSPRQVATIIRYLGEP"
     CDS             3982..4485
                     /locus_tag="ctg230_8"
                     /transl_table=11
                     /translation="MAMTVTYSVVPRKNPAKKDESAKYYAQAQASGELDFEELCEGITS
                     RSTCTETDVRAAISGILYEAKRALKAGRIVRLGDLGSLQIGLNSEGAVSVKEFSSSLIT
                     AAHIIFRPGKTLADITKILSYQQVTTRAVAQTGGSGNEGEDDDKGSGGGSDGGGSGEAP
                     DPAA"
     CDS             4482..4673
                     /locus_tag="ctg230_9"
                     /transl_table=11
                     /translation="MITLILDQMKEIVTKILDVIMFLVPFFGKRKRNRIVREVRFNATH
                     KEVCNVKTTEREKDDEKD"
     CDS             4660..5103
                     /locus_tag="ctg230_10"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRADRDFTAKDVDTAHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDEDGKAADTRTSRQKESLHRLVRELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVKAETW"
     CDS             complement(5131..5859)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg230_11"
                     /sec_met_domain="Radical_SAM (E-value: 4.5e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(5875..7413)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg230_12"
                     /sec_met_domain="Gly_radical (E-value: 2.9e-47, bitscore:
                     149.4, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 3.3e-233, bitscore:
                     764.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYM
                     EYELSKGTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGR
                     TKVTKTSFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLM
                     REVRQSDDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPV
                     LTSDTLNFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLA
                     YGVAGLSIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVY
                     FFSEELKKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDK
                     NGAIASLSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHL
                     NVNVLNREMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 ccggaatggc ttttttataa tcaattaaat gaaaagtttt atggaaaatt catgtaatga
       61 tgaattgttt tgttcattga aaaatgaact tcgtgaagta accgcacaaa tcaatgtgca
      121 tagtgaaaaa ttaatggagc tgctcgacca aaaaaaagaa tcgtcagtca actgactcga
      181 gagatgtcgc atctcacgcc tgtcaccatt gagtatcggg gaaatccgaa gcaatatgtc
      241 tctgttgtgc tcgatgccat caatcggggt aggttgacgt atgacggaat agcaaattgc
      301 gaacagactt ttcgtgcgtt agcaagcgtt gtagatgtga taagccctaa gaatgggaaa
      361 acgcttagtg tagagacgct ggtgtcttat gagaagaaaa agcgtgccgg agaatttgag
      421 gaaaaataat ataaaataaa aagacgaatg ttgtatagtt gaacaacaat tcatatattt
      481 gcagtatgaa aagagaaata atagcatata aaggatattt caaagagttc tttgatcgtc
      541 tggatactgg cacccaagat aagatattat atgtgttaat gctattgcaa acgcaggaca
      601 gaataccttt gaagtttatg aagttgatag aagaaggact ttatgaactt cgtattgagt
      661 accagagtaa tatatataga atattctttt gtttcgatga aggacgtatt gtgatactct
      721 ttaacggatt ccaaaagaaa acagaaaaga ccccaaagaa agaaattgaa aaagcaaaga
      781 tattaagaaa ggagtattat ggaagcaaaa acaaatgagg aattctttaa cgtcagcgca
      841 ttaatagatg agcgttttgg taaggaagga actgttactc gtgcggaagc agaagaaaaa
      901 gcgtatgctt tctacaccgg gcaaattatc gaagacgcaa ggaaaaaggc taagattact
      961 caagcagagt tagcccgacg tatcggttcc gaccgttctt atatttcaag agtggaaagt
     1021 ggtcagacag agcctaaggt ttctaccttc tatcgcatca tgaatgcgtt aggttgtagg
     1081 atcgaatttt cgatgtcttt ataagaagcg attaaaaaaa catggtggga aatttgaaaa
     1141 agttaactgt ctttttcaaa tttcccaccg tttttttgag gaagaaagag gaagaaaaag
     1201 gaaaaaggtg agggtaggtg agggtaaatt tctaacattc gtattttttt ttagaagaaa
     1261 atagaagaat agaaataaga aatataaaaa tgaaaagttg caaaagtacc ctcaccaacc
     1321 ctcacctttt tgcgtgcgaa ggagatgaaa tcttattcat atagcctgtt tgatttcacc
     1381 tgttccgtat caataatgaa tacagtgtat cttctcccgt tttttccctt atgaaattcg
     1441 aagccgtgtt tggacatcac ttttcctagt tgggctttgg ttccgtgaga atattgatac
     1501 cccgtccgtt cgcgaatcaa ttccgcaatt tcagtgaccg tcagataatg caccttttcg
     1561 aaacgttccg gtttgcggat atgtgtcaac accagttctt cttccggtga gtggaaaatg
     1621 aaatcctcgt tgttctcttc aatccgtgag ttttccgact gggtgaacca atactgatac
     1681 cccggcttgt tgagtagata cttgatttgt gcatacaact gcgcgtagtt tattttgata
     1741 aactccatcg agtcgacatg aaagcagaga aaacggcggt tacctgtggt gtcgtgaagc
     1801 acttcctggt aattgcatgt gccggcaaaa gaagccgtat gaggaaagtt ctgcgaacgc
     1861 cgcgcgtatg gcaggcggat gctgattact ttgcgggtga ctaaatcctt gaagatattc
     1921 agttcccgtc cgctcatccc ttcaaactca tccagattga taagcatcgc ctgcgctatt
     1981 ttagccagat catctttgtt gctcgggttg ataagaccgg tagaaagata ggcgcgcaat
     2041 tcgggcggca gcaggttgtt gataaacgta gtctttccga tgttctgtat ttcgctgcaa
     2101 agcaacaaaa cagtgtggtt caccacatca tcttgtgtgg cggcagcgca cattcccacc
     2161 agataacgtt ccagacattc tacccaaaat ttctggtgac tagtatgcac gctgttggca
     2221 aggataccga tataatccgt tccgtcccat tcaggcagcg agtctaaata ctcgcgaata
     2281 ggatggtagg tgatgctgaa gtcggagtag atcagattct gaatcgtctt gacggtgcac
     2341 gaatagccca gttcgttcat ctccatccaa atgctgtttt ccatcatttc gtcgagaata
     2401 tggaaaggtt ccggttgctc cgtgtccgtt ttccttctgc gatattccat gatatgaagc
     2461 acttcgttgt agcgtgtatc gtaattttct ttgatatgtt gctcaatctg taacattcgc
     2521 ttctgcgtac tattcagctt gcgggtgtcg aattcctcgt tgtgtccgta agcactcccg
     2581 atcagtgcgt ccatttcatc cgcgggaaga tcggtgaact ggctcttgat aaaagctgcc
     2641 gcctcttcct gggggacgcc gtagcgattg tacatacaag ccaggcaatg caggtagttg
     2701 ttccggttgc cggtgacgta cttttcggat ttattgtgat aatagttcag agtcaccatt
     2761 aaggaagtgt gcgaggaatg gtagttgagg gccacttgtt cggtgagggg attgttgtct
     2821 ggtgctgtcg tctttttctt tttgccggat gcccttttct tcttttgaga cttgaagaac
     2881 atcggtggct gttccacgat gacagctgtg gcatcgggat tgaaataaat atccggatcg
     2941 taagagaaca ggcaagtgcg gctaacatcc tgcccggaag tgtcgacctc aactccgcag
     3001 agctgggcat aaaaggaagc gattttgtga taagcagcat gatggaaatc ttcaatttcc
     3061 tgaattgttt cgggcaatgt cccgtctgtc cggcatacac gtaccacgat tttcagtccc
     3121 attcctttgg ggctgatgaa agcgatgcgg gtgtgggcac acttttttgc cagtgcgagg
     3181 atgcgtgcca atgcttcctg tgtctgatgg tcgatgtcta ccacgatgta tcccagatac
     3241 ttgacgagag ttgcgtttga acgtcctccc cggtagttgg ctgaaaaggt gatttctggt
     3301 agttctattt tcttttcgtt ggctgctttc tcgccttgtt ccgcgaagat tcggcgcacg
     3361 tcctccaccg tttcccggta tgtgccgttg ctgatggccg atacgatttc ttcttctgtc
     3421 atttcggcta taacgcggta aaagtcttga aagatggtaa aatgatccat gttggtttgt
     3481 tctttttaat ttgcggttgc aaagaaattt gtttgtaaaa taatagaatg gaacttttcg
     3541 tttttttgat taagttccta gggttctccc aaatagcgta tgatcgttgc cacttgccgg
     3601 ggagagagaa ggcggttgcg ggcacgatag ccttcttgtt cgagttcttg caggagcgga
     3661 gggttgtaga caatccaacg acgtagtatt tgtaaggcta ctttaaggca tacgttgggg
     3721 ttgtaaagat gagctagttc cgacttttta taagtgcgga tatgaaacgg ctggtccgga
     3781 taatacatga tgaataatgt ttggtttatg ctataaagtt actgaaaatt aatggtttat
     3841 taaaagaata acgtcattat tattcataga gtatcaggaa acttcgttgg ggaatccgga
     3901 ggatggaaaa tgtgttatct ttatgtcgtt aacaaataag gaacgcgttc gaaatgtgtg
     3961 aaatcttaaa attattttat tatggcaatg acagtaactt attcagtagt gccgcgcaag
     4021 aatcctgcca aaaaggatga gtcggcaaag tattacgcgc aggcacaggc ttcgggagag
     4081 ttggattttg aagagttgtg cgaaggaatc accagtcggt ctacctgtac ggagacagat
     4141 gtgcgtgccg ccatttcggg tattctttat gaggcgaagc gtgcgttgaa agcgggaaga
     4201 atagtccggc tgggtgattt gggcagtttg cagatcgggt tgaacagtga aggagctgtg
     4261 tcggtcaagg agttttcaag ttcgctgatt acggctgccc atattatttt ccgtcccgga
     4321 aagacgttgg cggacatcac gaagattctt agctaccagc aggtaacgac gcgtgcggtg
     4381 gctcaaacgg gcggtagcgg caatgaagga gaagacgatg acaaaggttc cggtggtggt
     4441 tcagacggtg gtggaagcgg agaagctccg gatccggcag catgataact ttaatattgg
     4501 atcaaatgaa agaaattgta actaagattt tggatgtaat tatgttcctt gtgcctttct
     4561 tcggaaaaag aaagcggaac agaattgtga gagaggtgcg cttcaatgct acccataagg
     4621 aagtgtgtaa tgtgaaaacg acggaaaggg agaaggacga tgagaaagat tagtctgatt
     4681 gtgattcact gctctgctac ccgtgctgat cgcgacttca cggcgaagga tgtggacact
     4741 gcccatcgct ttcggggctt ttcgtgttgg gggtatcatt actacattcg caagtcggga
     4801 cagatagagc cgatgaggga tgaagatacg gtgggtgctc atgcacgcgg ttttaatgcg
     4861 attagtttag gggtgtgtta tgaaggcgga ctggatgagg acggaaaggc agcggatacg
     4921 cgtacttccc gccagaaaga atctttgcat cgcctggtgc gcgaactgtt gcagcgttat
     4981 ccggatgcaa aagtggtggg acaccgtgat ttgagtcctg acaccaatta taatggaatt
     5041 gttgaccctt gggagcggat caaagagtgt ccctgtttcg aggtgaaagc agagacatgg
     5101 tgatttttat cctatttatt actgtggtgg ttaattgacc accacagtag tgaaatactc
     5161 tttaaatacc tccgcagctt tttcaagttg ttcgggggta ttttctttta cgtctttcag
     5221 cttgtattcc tgctccatcg cttcatactt atgtacgcct aatgtatgat aaggcaggat
     5281 ttccactcgt tgcaccatct tatattttcc cagagcttct cctaaacggc ggatgtcttc
     5341 ctcgaaatcg ctgtatccgg gcactaatac ataacgcagc cagaaaggtt tctcgttttc
     5401 ttccagccat gctgccgtgc ggatggtttg ctcgttactt cttccggtca gcgcttgatg
     5461 gcgtgccgga ttgaactctt taatatccag caatacaaga tccgtcagtt tgaaaagttc
     5521 ttcaacttct tcgttccaga taccgccgtt gctgtctatg catacatgta ttcccttttc
     5581 tttcagttca cgcactaaag ggactagtgc tttggcctga aacgtaggtt ctcctccgga
     5641 gaaagtgact cctccgcgtt tcccgaaaaa aggacgttgg ctcatagcca tgcggacgat
     5701 ttcttccggt ggagtaggtg tgcctccttt cccggctatt gtgtcagggt tggcgcaata
     5761 caggcagcgg aaattgcatc cttgaagaaa aacgacgagc cgtaagcccg gcccgtcgaa
     5821 tgttcccata ctttcgtatg aatgtacgtt tatcgtcata ttgagtagat ctgattacat
     5881 acgttcgtgg aagctacggc tgataacttc caattgatgt tcgcggctca acttcacgaa
     5941 gtttacggca taaccggaaa cacggatggt gagttgcgga tatttttccg gatgttccat
     6001 ggcatcgtaa agcatttcac ggttcagtac gttcacgttc aggtggtgag cgcctttggt
     6061 gaaatagcca tccatcattg tcaccagatt ttctacgcga tcctcttcag tagctcccag
     6121 tgatttgggg acgatggaga aagtgttgct gataccgtcc tgtgagtcgc ggtaacgtaa
     6181 tttcgctacg gaactcaaag aggcgatggc accgttcttg tcacgtccgt gcatagggtt
     6241 ggctcccgga gcaaaagcga cacctttggc gcgtccgtcg ggagtagcac ctgtcttctt
     6301 gccatacatc acgttggaag tgatggtgag caaagataaa gtaggacggg catttttgta
     6361 aaccggaagt ttcttcaatt cttcgctgaa gaaatagacc aggtcaacac ccaggtggtc
     6421 taccttgtcg ttgtcattac cgaagcaagg gaattcgcct tgaatatcga aaccttctgt
     6481 cagaccgatg tcgttgcggc gtgcagttac tttggcatat ttgatggccg acagtgaatc
     6541 gagggcgata gaaagtccgg ctacaccata agcaaggttg atgcgtgggt tggtatctac
     6601 aagagccatt tgggcttttt cgtagtaata cttatcatgc atataatgga tgatgttcat
     6661 cgcttcgttg taaacacggg cgatttctgt caataccttc ttgtagttgc tcattacttc
     6721 ttcgaagttc agtgtgtcgc tggttagtac ggggatattt ttcaccatta ccgtaccggt
     6781 gttttcgcaa cgtccgccgt tgatggcaag cagcagggct tttgccaggt tgcaacgtgc
     6841 gccgaagaat tggatttgtt ttccgatttc ctgataagat acgcagcatg cgattccgta
     6901 gtcgtcagac tggcgtactt cacgcatcag gtcgtcgttt tcatattgga tggaagaagt
     6961 gtcgatagat acttttgcgc agaattcttt gaatccttcc ggaagttccg ggctccacaa
     7021 tacagtcagg ttcggttccg gtgaagggcc gaggttgtag agtgtttgca ggaagcggaa
     7081 agaagtcttt gtcaccttcg tgcgtccgtc gttgaggcgt ccgcccaggg attcggttac
     7141 ccatgtcggg tcgccggcaa agatgtcgtt gtaagattgc atacgcaggt ggcgcaccat
     7201 acgcagtttg atgacaaatt ggtcgatcag ctcctgtgcg aaagattctg tgatggttcc
     7261 tttgctcaat tcatattcca tataaatgtc gaggaacgaa gagacgttac ccagtgacat
     7321 ggcagcaccg tcttgttctt ttacggcagc aaggtaagcc atgtataccc attgtactgc
     7381 ttcttgtgcg gtgtaggcag gacggctcaa gtcg
//
