LOCUS       gnlProkkaLHDHOLGL_7    13569 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LHDHOLGL_7.
ACCESSION   gnlProkkaLHDHOLGL_7
VERSION     gnlProkkaLHDHOLGL_7
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-01 18:19:27
            Original ID  :: gnl|Prokka|LHDHOLGL_7
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 117169
            Orig. end    :: 130738
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..13569
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:8569]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..8569
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..13569
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..13569
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             complement(30..1166)
                     /NRPS_PKS="Domain: NAD_binding_4 (99-186). E-value:
                     3.8e-09. Score: 27.9. Matches aSDomain:
                     nrpspksdomains_ctg7_104_NAD_binding_4.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score: 86.6;
                     E-value: 3.4e-26)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg7_104"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 5.2e-09,
                     bitscore: 28.5, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGARGFVGRNLVSQLHNIRDGKARNYGIASEELAIYEYDMDSDP
                     AELDVYCRQADFVFNLAGVNRPQDASEFMKGNFGFASTLLDTLRKHGNTCPVMISSSTQ
                     AALDNPYGESKRAGEQLLFEYSKETGAKVLVYRFPNVFGKWCRPNYNSAVATFCHNIAH
                     DQPIRVNDPSVVMNLVYIDDVVDELVSAMGGREHRNGDYCEVPVVHTITLGGIVELIRS
                     FRQMPGTLSVPDLSDAFTKKLYSTYLSYLPEERFSYPLKMNVDDRGSFTEIIRTSDRGQ
                     FSVNISKPGVTKGQHWHHTKNEKFVVVSGHGLIQLRKIGTEEVVSFEVNGGRMEVVEMI
                     PGYTHNIVNLSETEDLVTFMWCNECFDPARPDTYFEKV"
     aSDomain        complement(609..869)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg7_104_NAD_binding_4.1"
                     /evalue="3.80E-09"
                     /label="ctg7_104_NAD_binding_4.1"
                     /locus_tag="ctg7_104"
                     /protein_end="186"
                     /protein_start="99"
                     /score="27.9"
                     /tool="antismash"
                     /translation="SSSTQAALDNPYGESKRAGEQLLFEYSKETGAKVLVYRFPNVFGK
                     WCRPNYNSAVATFCHNIAHDQPIRVNDPSVVMNLVYIDDVVD"
     CDS             complement(1219..1623)
                     /locus_tag="ctg7_105"
                     /transl_table=11
                     /translation="MNRIVLDTNSLIQCISPKSRYRKIWDCFLDGTNQLCVSDEILNEY
                     EEILERLAGVEIAKLVIDVILNSPYTLFFTPYYNFNLIKSDPDDNKFVDCAVVANAKFI
                     VTEDHHFHILKQYNFPKVSFIGLDDFCHSL"
     CDS             complement(1620..1841)
                     /locus_tag="ctg7_106"
                     /transl_table=11
                     /translation="MAATVLNSVQQHLLKMFAFDGSEERLNEVKEVLTKYFSQKLDDRL
                     NDLWDSGVLNQEKLDELRKIDVRTLPKR"
     CDS             complement(1942..3015)
                     /NRPS_PKS="Domain: NAD_binding_4 (10-144). E-value:
                     4.5e-07. Score: 21.1. Matches aSDomain:
                     nrpspksdomains_ctg7_107_NAD_binding_4.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     105.1; E-value: 7.9e-32)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg7_107"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 4.9e-108,
                     bitscore: 353.6, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.3e-07, bitscore:
                     23.9, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVFKDKVLLITGGTGSFGNAVLRRFLDSDIKEIRIFSRDEKKQD
                     DMRHHLQNPKVKFYIGNVRDRQSVDGAMMGVDYVFAAAALKQVPSCEFFPLQAVQTNVI
                     GTDNVLESAIAHGVKNVVVLSTDKAAYPINAMGISKAMMEKVAIAKGRALGEEAGTTIC
                     CTRYGNVMASRGSVIPLWVEQIKNGRPITVTDPNMTRFMMTLDDAVDLVIYAFEHGHNG
                     DLFVQKAPAATLDVLAQALKETYAQVDPKYGETEVKTIGTRHGEKLYETLVTREEMAKA
                     VDMGDYYRIPCDTRDLNYDKFFTEGNEDIARIEDYHSHNTARLDVEGMKKLLLKLRFIR
                     EDLGMEEKAKSAEIKSE"
     aSDomain        complement(2584..2985)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg7_107_NAD_binding_4.1"
                     /evalue="4.50E-07"
                     /label="ctg7_107_NAD_binding_4.1"
                     /locus_tag="ctg7_107"
                     /protein_end="144"
                     /protein_start="10"
                     /score="21.1"
                     /tool="antismash"
                     /translation="ITGGTGSFGNAVLRRFLDSDIKEIRIFSRDEKKQDDMRHHLQNPK
                     VKFYIGNVRDRQSVDGAMMGVDYVFAAAALKQVPSCEFFPLQAVQTNVIGTDNVLESAI
                     AHGVKNVVVLSTDKAAYPINAMGISKAMME"
     CDS_motif       complement(2932..2988)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg7_107_0001"
                     /evalue="1.70E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="ctg7_107"
                     /protein_end="28"
                     /protein_start="9"
                     /score="11.1"
                     /tool="antismash"
                     /translation="LITGGTGSFGNAVLRRFLD"
     CDS             complement(3030..4145)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg7_108"
                     /sec_met_domain="Glycos_transf_1 (E-value: 6e-12, bitscore:
                     38.5, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNILINCSNLKKGGGLQVADSVCSCLNTIPQHDFFLVLSSCMDDT
                     ADRVSEYKNVTVVRHDIRNSWYTLFWGRDKAMDNLVTRHNIGGVLTIFGPARWKPKCKH
                     LCGFARSHLVLNDSPYFQRMSWKELMIEKARNLYLYYYFKRGVTAFWTENPYISEKVKH
                     LFPTIKAYTVSNYYNQVFDDENTQIEHALPSFDGCTLLSINAPYPHKNLAIAIEVARYL
                     KKHYSEFSFRFVFTVTENDLPPIPSYLREHFCLIDKVSVAECPSLYRQADIMFQPTLLE
                     CFTATYVEAMRMQVPILTTDLPFAHGLCGNAAVYYSPLDAEDCAKSIYKMAVDTSFQDY
                     LVGEGIKQLKSFDNYNRRAEKLIDLISNLDD"
     CDS             complement(4300..5016)
                     /locus_tag="ctg7_109"
                     /transl_table=11
                     /translation="MSRGISEKEQRWLLVLSQVIEVLDKNNLLYFIDTGTLLGAVRDKM
                     FIPWDNDIDLAVIGGDTSTTNLLKVSKLLYALGFYVTLTKKEIAICSRDGVNLGIKLYK
                     KQASHYEACMGKVYRHRLFYSINLYLSGEVLYKKGFSFKYKAISLFVKFSQVFKLKLSC
                     IWQMKLLFWAKYDEKTVIIPFEFVDAIIDYNFYNIIVKVPNKPERYLAYRYGENWKVPK
                     QDYDYMIDDKSIINID"
     CDS             complement(5001..6095)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Fe-ADH"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg7_110"
                     /sec_met_domain="Fe-ADH (E-value: 1.5e-75, bitscore: 247.2,
                     seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQRFFLGESSIKELIPILQEYTPDHIFLVRGKKSYEKCGAQAVL
                     ESVFVKLACGVTEFFDFEANPKIEDMEKGLSLLKEYPTNMIIAIGGGSVLDMAKLLRFF
                     YSYSGNPTGGEFEKKRQLIPLITLPTTAGTGSEATHFSVLYKDKVKYSVEHDAILPDVA
                     IVYPKFTYGNSKYLTSCTGFDALAQAIEAYWNVNATEESDKYAAKAIKLLWPNLPLVVN
                     SPTAEARNKVSEGSYWAGRAINITKTTAPHAFSYSFTTDYGYPHGHAVALTFPFFMRYN
                     IGNSDILLNPNINPYLYKIKMQKMLQWLNMECVDVAIAMKEYIRNLGLDIDISQIDISK
                     IVSSVNIQRLINNPYKVEAICLEE"
     CDS             complement(6103..7233)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 69;
                     E-value: 6e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg7_111"
                     /sec_met_domain="TPP_enzyme_N (E-value: 1.8e-16, bitscore:
                     53.2, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 3e-20, bitscore:
                     65.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISPNIFVDSLGAMQMDFFTGVPDSLLKNICAYLTDHLASEHNII
                     AANEGAAIGLATGYYLATRKIPVVYMQNSGLGNAVNPLMSLTDKEVYNIPLLLLIGWRG
                     EPGVKDEPQHIKQGKVTLPLLEAMGIRYAVLSQDEKKFISQLNVAKSYMDETREPFAFV
                     IQKDTFLEYSSENCEDERLSLKREAAIHFIAASLTEKDIIISTTGMISRELFEYRESTS
                     LEHERDFLTVGSMGHASQIALGIALKKRDCKVYCFDGDGSTLMHMGSLAIVGSLHPENY
                     VHVVFNNGAHDSVGGQPTVALDIDLCKIAQACGYEIVLSVSDNEGLCEALKKIQQIRGP
                     ILLEVKVRKGARKNLGRPANTPIQNKEAFMLFLDNL"
     CDS             complement(7265..8569)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 202.6;
                     E-value: 1.3e-61)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg7_112"
                     /sec_met_domain="CTP_transf_like (E-value: 3.2e-12,
                     bitscore: 39.8, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 8.2e-119, bitscore:
                     388.9, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 2.7e-63, bitscore:
                     206.6, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKKKVYVGMSADIIHPGHLNIIHEASKLGHVTVGVLTDSAIASY
                     KRLPYLNFDQRSLIVLNLKGVDEVIPQTTLDYVPNLELIRPDFVVHGDDWKEGIQQETR
                     QRVIDTISKWGGKVIDVPYTKGISSTLLNTRLKEIGTTPEIRLKRLKRLIAAKSIVRIC
                     ESHSGLTGLIIENTSVEVNGVKREFDGMWSSSLTDSTSKGKPDIEAVDLTTRLHDLNDA
                     LECTTKPVIFDGDTGGKIEHFVFTVRTLERLGISAVIIEDKIGLKKNSLFGTDAVQTQD
                     TIEGFCDKIQAGKRAQITDDFMIIARIESFIAGKGLDDALGRALAYIKAGADGIMIHSK
                     DKSGEDIKNFCLALRKIHKSIPIVVVPTTYNHISEDELASWGVNVVIYANHMLRSAYPA
                     MLNTAKSILANARSYEANDLCMPIKEILELIPGTK"
     CDS             complement(8532..9911)
                     /locus_tag="ctg7_113"
                     /transl_table=11
                     /translation="MIAIIFLILFLIQILISNRLIIDSFSRKISFIYLLWWGTAIVIST
                     FDPAELYSVSMKTYMLLLINVWAFNWGFLCFGKIKKIRLKVTTDNSFYNLVIGNNKMIL
                     LLMLACIVTYYYAMKFALVIELYGAGEGRMMRFEAGELFTSNLEVYLYDFLIKPFTQFV
                     MLIVACMLLSKIKRRILLLSVLFLFLYASVGNGRGGLIVFLFYCFIIYYLRRELYYINL
                     LASKRKKWNFGVVSFVCLFVGVGIIVVVISYMTAQRMGFDTFSAEVVKIGFEELKSQAV
                     WYFLGPFRALDYSFRANYESITGFWYGRATMGGIDNLLKLVIHYLGGSYTIAHDEIGHL
                     LQNSKISVGNGIIFNYAYTNIIFHYLDFGYVGVLLFPFLYGFIVRYSIYKFTQKPTLLT
                     LGIMIYLVYISMFNIFSWEFQKPSSIIYVLLLLFFQYVYSHKFVILKLNGKKKGLCRHE
                     C"
     CDS             complement(9908..10840)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg7_114"
                     /sec_met_domain="Glycos_transf_2 (E-value: 7e-25, bitscore:
                     80.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNGSITVSICCLTYNHEKYIRQCLDGLIMQKTEFDFEILVHDDAS
                     SDATQSIINEYVVAYPNLIKPILQIRNQYSQGISPLRQILFPLVKGKYVALCEGDDYWT
                     DPLKLQKQVNAMESDHSLAGCAHQSLVFYENGTPSHMFHHVKKQILKTKDLLDVRLFHT
                     ASFLFKKEVLDYIKDFNSSLYAGDRFLFLACSLKGNILYMPDVMCCYRKNAMGVTSRVS
                     YSLHVLDLKVPEYFHNKDLKFPYYRYLAFVHRAIFSESLDISLKKYIYHFTLFVKYSFS
                     YFPKNLKDIYKALYVSFWWLTKGQKYRIK"
     CDS             complement(10841..11836)
                     /NRPS_PKS="Domain: NAD_binding_4 (91-233). E-value: 3e-07.
                     Score: 21.7. Matches aSDomain:
                     nrpspksdomains_ctg7_115_NAD_binding_4.1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     114.5; E-value: 1.1e-34)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg7_115"
                     /transl_table=11
                     /translation="MKKKIIIFGATGNTGSYLLEYALNFFNKEEYEIIASGRRETTFFS
                     KRGIPYYSVDITKEEDFDKLPMENIHAVMLLSAQIPSYMDTYCPIKYLDSIVIGGFNVL
                     EYCRKCHVDRIIYTQTVFDISLYSHDTILKPDMKPNFSYTGDHAIYVISKNTALDLIEH
                     YYQEYGLKKFIFRLPTIYSYSPYPYYFPNGVKTKRPIYQMIENAQKGLPIEIWGDPHYA
                     KDMVHVNDFSQMLCKAVEVEQESGFYNVGTGMPITLEEQIRTIISVFSPKESPSEVIYR
                     PDKVCGGGYIMNIENAKKELGYVPQYDCRKLFEDYKSEMEIKRFAELRLK"
     aSDomain        complement(11138..11563)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg7_115_NAD_binding_4.1"
                     /evalue="3.00E-07"
                     /label="ctg7_115_NAD_binding_4.1"
                     /locus_tag="ctg7_115"
                     /protein_end="233"
                     /protein_start="91"
                     /score="21.7"
                     /tool="antismash"
                     /translation="YLDSIVIGGFNVLEYCRKCHVDRIIYTQTVFDISLYSHDTILKPD
                     MKPNFSYTGDHAIYVISKNTALDLIEHYYQEYGLKKFIFRLPTIYSYSPYPYYFPNGVK
                     TKRPIYQMIENAQKGLPIEIWGDPHYAKDMVHVNDFSQ"
     CDS             complement(11833..12795)
                     /locus_tag="ctg7_116"
                     /transl_table=11
                     /translation="MSIGGYFELEIREGEHFHQGAIKLSTARQCFEYILKARRYTKVYI
                     PYYTCDVIIEPLMKLNVKYEYYHINSLLDPVFDKKIESSEAFLYTNYFGLKQDTVLYLS
                     RFYPNLIVDNAQAFFAKPIEGVDTFYSARKFFGVPDGAYLYTTALLDIDFPIYNPIDNI
                     NYLIGRITDSAEGYYKDFQESEERMSNQEIHRMSVLSDKILSSLDYVSIIERRRFNYHL
                     LYSALTAFNKFDFSISENQNVPLIYPFYTSKAGLRQQLILEHVYVPQYWQNVVNKIDAK
                     CLEYSIANNMIALPIDQRYDEKTMNILIEKISNLNNHLS"
     CDS             complement(12795..13484)
                     /locus_tag="ctg7_117"
                     /transl_table=11
                     /translation="MKLGIMQPYFMPYIGYFQLIKAVDKYVVYDDVNYIKGGWVNRNNI
                     LVNGEKKMFTITLKGASAYKHFNEIEIGDDFRKFMKTLELNYSKAPFYHETMELLYRII
                     MFPNRQLALFLANSFKEILDYLNIQTEILLSSDLPKDCSLKGKDKVLHICKLLSADTYY
                     NAIGGKQIYNKEDFMQNGINLKFVDTIIKRYPQQSKEFVPYLSIIDVLMNNSKEETCML
                     LDSFSLQ"
ORIGIN
        1 ccatctgttc ctatattctt taactcgttt cagacttttt caaaataagt gtccggacgt
       61 gccgggtcga aacactcgtt gcaccacatg aaggtcacca ggtcttccgt ttccgaaagg
      121 ttgacaatgt tgtgcgtata gccgggaatc atctccacga cctccatcct cccgccgttt
      181 acctcgaagc tgacaacctc ctccgtacct atcttgcgga gctgtatcag accgtgcccg
      241 ctcaccacca caaacttctc gttcttggta tggtgccagt gctggccttt cgtaacgccc
      301 ggcttggaga tgttcaccga gaactgcccg cggtcggaag tacggataat ctccgtaaaa
      361 ctgccgcggt cgtccacatt cattttcagg ggatagctga accgttcttc aggcaggtat
      421 gagaggtatg tggaatagag tttctttgta aaggcatcgc tcaaatccgg gacggacaag
      481 gtaccgggca tttgtctgaa agaccttatc agttccacaa tgccgcccaa ggtaatggta
      541 tgtactacgg gcacttcgca ataatcaccg ttgcggtgtt ccctgccgcc catggcagag
      601 acaagctcgt ccaccacatc gtctatgtaa accaggttca tcaccaccga agggtcgttc
      661 acccggatgg gctggtcatg ggcgatgtta tggcagaaag tggccaccgc gctgttatag
      721 ttggggcggc accatttgcc gaaaacattc gggaagcggt acaccagtac cttcgccccc
      781 gtttccttgg agtattcgaa caacagttgc tcccctgccc gcttcgattc accgtaaggg
      841 ttgtcgagtg cggcctgcgt ggaagaggag atcattaccg gacaggtatt gccgtgtttc
      901 ctcaatgtgt cgagcagcgt ggaggcgaag ccgaagttcc ctttcataaa ctcagacgca
      961 tcctggggac ggttcacgcc cgccagattg aagacaaagt cagcctgcct gcaatagaca
     1021 tccagttcgg cagggtcact gtccatgtca tactcgtaaa tggcaagttc ctcacttgca
     1081 atgccgtaat tgcgggcttt cccgtcacgg atgttgtgca gctgggatac aaggttccgg
     1141 ccgacgaacc ctcgggcgcc ggtcactaag attttcataa actaatgggt tctgtttgtc
     1201 acagaaaaga gaagatattc atagtgaatg acagaaatca tccaaaccta taaaacttac
     1261 ttttgggaaa ttatattgct tcaatatatg aaaatgatga tcttccgtaa caataaactt
     1321 ggcattagcc acaactgcac agtccacaaa cttattatca tccggatctg attttatcag
     1381 attgaagtta taataaggag taaagaacaa agtataaggg ctattgagaa tgacatcaat
     1441 caccagctta gctatttcca cacctgccaa acgttctaat atttcctcat attcatttag
     1501 aatttcatca gaaacgcata actgattcgt tccatccaga aagcaatccc aaatcttacg
     1561 gtacctgctt ttgggagaaa tgcactgtat caggctattt gtatctaata caatcctatt
     1621 catctcttcg gaagtgtgcg gacatcaatt tttctcagtt catcaagttt ttcttggttt
     1681 aaaactcccg aatcccataa gtcattcaag cggtcatcta acttctgaga aaaatatttg
     1741 gtcaacactt ctttaacttc attcaatcgc tcctccgaac catcaaaagc aaacatcttc
     1801 aaaagatgtt gctggaccga atttaatact gttgctgcca tattctcaat tattagtttc
     1861 aatatggcaa agatagtttt tttttctaaa ggagcaatac attccctctt tctttcctgt
     1921 tgtattattc aaattaaaga cttattcact cttgatttcc gcactcttcg ccttttcctc
     1981 catcccgagg tcttcgcgga tgaagcggag tttcaaaaga agctttttca tgccctccac
     2041 atccaggcgg gcggtgttat ggctgtgata gtcctctata cgggcaatat cctcattgcc
     2101 ttccgtgaag aacttgtcat agttcaggtc acgggtgtcg caagggatgc ggtagtagtc
     2161 gcccatgtcg acagcctttg ccatctcttc gcgggttacc agtgtctcat acagtttctc
     2221 gccgtggcgc gtgccgatgg tcttcacctc cgtttcaccg tatttcggat caacctgcgc
     2281 gtatgtttcc ttcaaggcct gcgccagtac atccaaggtg gcggcaggag ccttctgcac
     2341 gaacaggtca ccgttatggc cgtgctcaaa cgcatagatt accagatcca cggcatcgtc
     2401 cagcgtcatc atgaagcggg tcatgttggg gtccgttacc gtaatgggcc tgccgttctt
     2461 tatctgctcc acccacaagg gaattacaga accgcggctt gccatcacat tgccgtaacg
     2521 ggtacagcag atggtcgtac cggcttcctc acccaacgca cggcccttgg ctatggcaac
     2581 cttctccatc atggccttgc tgatacccat ggcattgatg ggataggcgg ccttgtcggt
     2641 agaaagcacc accacattct ttacaccatg cgctatggca gattccagta cattgtccgt
     2701 accgatcaca ttggtctgca cagcctgcaa cgggaagaac tcgcaactgg gaacctgctt
     2761 caaggcggca gcggcaaata cataatccac tcccatcatg gcaccgtcta cagactggcg
     2821 gtcacggaca ttaccaatgt aaaattttac tttgggattt tgcaggtgat gacgcatgtc
     2881 atcctgcttt ttctcgtcac gactaaaaat acgaatctcc ttgatatcag aatcaaggaa
     2941 acgacgcaat acagcgttac caaaactgcc ggtacctccg gttataagga ggactttgtc
     3001 tttaaatact gacataaata taatatgtat taatcatcca aattcgatat caaatcgatt
     3061 aatttttctg ctctccgatt ataattatcg aaggacttaa gttgttttat tccttctccg
     3121 accagataat cttgaaagga tgtgtctaca gccattttat aaattgattt ggcacagtct
     3181 tctgcatcta acggcgaata gtacacagct gcatttccac ataatccatg agcaaatggc
     3241 aaatctgtag ttaaaattgg cacttgcatt cgcatcgctt cgacatatgt ggcagtgaaa
     3301 cattcaagta aagttggttg aaacattata tctgcctgac gatataagga agggcattct
     3361 gctacactaa ctttatcaat cagacagaaa tgctctcgca aataagaagg aataggtggc
     3421 aagtcattct ctgtcaccgt aaaaacgaat cgaaaactaa attcggaata atgtttttta
     3481 agatatcgag cgacttcgat agcaattgct aaattcttat gaggataagg agcatttatg
     3541 gacagtaaag tacaaccgtc aaaagacggt aaggcatgtt ctatttgagt attttcatca
     3601 tcgaaaacct gattataata attcgataca gtataggctt ttatggtagg gaacaagtgc
     3661 tttactttct ctgaaatata aggattctca gtccaaaaag cagtcacacc acgtttgaag
     3721 taataatata aataaagatt tctcgccttt tctatcatta gttccttcca agacatacgt
     3781 tgaaaatagg gagaatcgtt caaaacgaga tgggatctgg caaatccaca tagatgctta
     3841 catttcggct tccatcttgc aggtccgaat atggtaagaa ctccaccaat attgtgtctg
     3901 gttaccaaat tatccatagc cttatcacgt ccccaaaaca atgtatacca actgtttcgt
     3961 atatcatgac gcactacagt gacatttttg tattccgata ccctatcagc agtatcatcc
     4021 atacatgagg ataatactaa aaaaaaatca tgttggggaa ttgtattcaa acaagaacaa
     4081 accgaatcag cgacttgaag ccctccacct ttcttgagat tggagcaatt tattagaatg
     4141 ttcatttgtt ccgagaatat aatatataag tgcccaattt tggtgtataa atgaaagtgc
     4201 tatttataaa taccatctat attgtctaaa gaaaaagttg attataattt tattaattac
     4261 aataatactc cttatgttga aatttgtttt catatttttt taatcaatat ttataataga
     4321 cttgtcatca atcatataat catagtcttg tttgggaacc ttccaatttt caccatatct
     4381 atatgctaga tatctttctg gcttatttgg cactttcaca attatattat aaaaattata
     4441 atcaataatt gcatctacaa actcaaaagg aataatcacc gtcttttcat catacttagc
     4501 ccaaaacaat aacttcattt gccaaataca agacaatttt aacttaaaga cttgactaaa
     4561 cttcacaaag agtgagatag ctttatactt aaaagaaaag ccttttttat acaggacctc
     4621 tccagagagg tataaattta tagaataaaa tagtctatgt ctataaactt ttcccataca
     4681 tgcttcataa tgacttgctt gctttttata taatttaatt cctaaattta ccccgtctct
     4741 agaacagata gcaatttctt tctttgtcaa agtgacgtaa aagcctaaag catataacaa
     4801 ttttgacact ttaagcaaat tagtagtaga tgtatctcct ccaataactg ctaaatcaat
     4861 atcattatcc caaggaataa acattttatc tctaacagct cctaaaagag taccagtatc
     4921 aataaaatat aataaattat ttttatcaag aacttcaata acttgtgata atactaacaa
     4981 ccatcgttgc tccttttcac ttattcctct agacatatcg cttcaacttt atatggattg
     5041 tttattaatc tttgaatatt aacagaagat acgattttcg atatgtcaat ttgagatatg
     5101 tcaatatcta atcccaagtt tcttatatac tctttcatag cgattgcaac atctacgcat
     5161 tccatattta accactgtaa cattttttgc attttaatct tatataagta aggatttata
     5221 ttaggattca ataagatatc cgaatttcca atattatatc tcataaaaaa gggaaatgtt
     5281 aatgctactg catgtccatg cggatatcca taatcagttg taaatgaata cgaaaaagca
     5341 tgtggagctg tagtcttagt tatattaatt gcacgaccag cccaataaga gccttccgaa
     5401 accttattcc tagcctccgc tgtaggagaa tttacaacca atggcaaatt aggccataag
     5461 agtttaattg ctttcgccgc atatttatcc gactcttccg tagcattcac attccaataa
     5521 gcttctatcg cctgcgctag agcatcaaag cctgtacaag atgttagata ttttgaattt
     5581 ccataagtaa attttggata tacaatcgct acatccggaa gaatagcatc atgttctact
     5641 gaatatttta ctttatcttt atataaaaca gaaaaatgag ttgcttcact acctgtacca
     5701 gctgtagtag gaagtgttat taatggaatt aattgtcgct tcttttcaaa ctcccccccc
     5761 gtaggattac cagaatagga ataaaaaaaa cgaagcaatt ttgccatatc taatacacta
     5821 cctccaccta tagcaataat catatttgtc ggatattctt tcaataatga aagtcctttt
     5881 tccatatctt caattttagg atttgcttca aaatcaaaga attccgtcac gccacaagct
     5941 aatttaacaa aaacagattc taacacagcc tgtgcaccac atttctcata agactttttt
     6001 cctcttacaa gaaatatatg atcaggagta tattcttgca agataggaat caattctttt
     6061 atagaagact ctcctaaaaa aaaacgctgt tccataatta ctctataaat tatctaagaa
     6121 aagcatgaat gcctctttat tttgtatagg agtgttggct ggtcttccca aattcttacg
     6181 agctcccttt cgaaccttta cttccaacaa aatagggccg cgtatttgct gtatcttttt
     6241 aagagcttca cataaacctt cgttatcaga aacagacaat actatttcat aaccacaagc
     6301 ttgagcaatt ttacataaat caatatctaa agcgactgtt ggttgtcccc ccacagaatc
     6361 gtgagcgcca ttattaaaga ctacgtgtac atagttttcc ggatgtaaag aaccgacaat
     6421 tgctaacgaa cccatatgca tcaaagtgga accatctcca tcaaagcaat aaactttaca
     6481 gtctctcttt ttaagagcta ttcccaatgc tatctgcgac gcgtgcccca tagaacctac
     6541 agtcaaaaaa tctctctcat gttctaaaga agtagattca cgatattcaa ataattcacg
     6601 tgatatcata cctgttgtcg atattataat atctttttct gttaatgaag ctgctatgaa
     6661 atgaatagca gcttcgcgtt tcaaagacaa ccgttcatct tcacaatttt cagaactata
     6721 ctctaagaaa gtgtctttct gaattacaaa agcaaaaggc tctcttgtct catccatgta
     6781 agattttgca acattaagtt gagatataaa cttcttttca tcttgagata aaacagcata
     6841 tctgataccc attgcctcaa gtaatggaag tgtgactttt ccctgcttaa tatgttgtgg
     6901 ctcatctttc acaccaggtt caccacgcca accaattagc aataacaatg gaatattata
     6961 aacttcttta tccgtcaatg acattaaagg atttactgca tttccaagac ctgaattctg
     7021 catataaaca actggaattt tcctcgtagc caaataatat cctgtagcca aacctatagc
     7081 agcaccttca ttagcggcaa taatgttatg ttcagaagca aggtgatctg ttaaatatgc
     7141 acatatattc ttcaataaag aatcgggaac tccagtaaaa aaatccatct gcatagcgcc
     7201 aagagagtct acaaaaatat taggagaaat cataataata agaatttaat tttaaagagt
     7261 ctatttactt agtccctgga atcaattcca gaatttcttt aataggcatg cataagtcat
     7321 tggcttcata agagcgtgca ttggcaagaa tggacttcgc cgtattaagc atagcgggat
     7381 atgcacttct aagcatatga tttgcatata ttacaacatt tactccccaa gaagccaatt
     7441 catcttctga tatatggtta taagtagttg gaacaaccac tatcggaatg gatttatgta
     7501 ttttgcgtaa agcaagacag aagttcttta tatcctcacc agacttatct ttgctatgga
     7561 tcataatccc atccgcacct gctttaatat atgctaatgc acgtcctaaa gcatcatcta
     7621 aaccttttcc agcaataaaa ctttcaatac gagctattat cataaaatca tccgttatct
     7681 gagcacgttt acccgcttga attttatcac aaaacccttc aattgtatcc tgggtctgaa
     7741 cagcgtctgt tccaaaaagc gagttcttct tcagacctat tttatcctcg ataataacag
     7801 ctgatatccc taaacgttcc agcgtgcgaa cagtaaatac aaaatgttct attttaccac
     7861 ctgtatcacc atcaaaaatt actggtttgg tagtacactc caaagcatca ttcaaatcat
     7921 gaagacgagt tgtcaagtca acggcttcaa tatcaggctt acctttagaa gtcgaatcag
     7981 taagactact ggaccacatc ccatcaaatt cacgcttaac tccgttgact tctacactgg
     8041 tattttctat tatcaaacct gttaacccac tatgagattc acagatacga acaatacttt
     8101 tagcagcaat taagcgttta agtcgcttca aacgaatttc gggagtggtt cctatttcct
     8161 tcaacctagt attcaataaa gtggaagaaa tacctttcgt ataaggaaca tctatcactt
     8221 ttccacccca tttagagata gtatcaatca cacgctggcg agtttcttgt tgaataccct
     8281 ctttccagtc atcaccatgt actacaaagt caggcctaat caattccaaa ttcggaacat
     8341 aatcaagagt agtttgaggt attacttcat caacaccctt caaattcaga actatcaatg
     8401 agcgctgatc aaaattcaag taaggcaaac gcttgtaact ggcaatagct gaatctgtta
     8461 atacaccaac cgttacatgc cccaattttg aagcctcatg aatgatattc aaatgacctg
     8521 gatgaataat atcagcactc atgccgacat aaaccttttt tttttccatt taattttaat
     8581 attacgaatt tatgagaata aacatattga aaaaatagca acaacaaaac ataaataatt
     8641 gaggaaggct tctgaaattc ccacgaaaaa atattgaaca ttgatatata gaccaaatat
     8701 atcataatac caagagtcag taatgttggt ttttgagtaa atttataaat actatatcgt
     8761 acgataaagc catataaaaa tgggaataaa agaacgccaa cgtaaccaaa atccaaataa
     8821 tgaaatatta tattagtgta tgcataatta aagatgattc cattcccaac tgaaattttt
     8881 gaattttgca aaagatgacc tatttcatca tgagcaatgg tataacttcc tcctaaataa
     8941 tggattacaa gttttaataa attatcaatt ccacccattg ttgcccttcc ataccaaaac
     9001 ccggttatcg attcataatt agctctaaat gaatagtcta aagctctaaa tggacctaaa
     9061 aaataccata cggcctgact ttttagttct tcaaaaccta tttttacgac ctcagcacta
     9121 aatgtatcaa atcccattct ttgagctgtc atatatgaaa taaccacaac tattattcca
     9181 acgccaacga acaagcatac aaaagaaact acaccaaaat tccatttttt ccttttgctg
     9241 gctaacaaat tgatatagta caattctctt cttaaataat aaatgataaa acaataaaat
     9301 agaaaaacaa tcaatccccc acgcccatta ccaacagatg cgtataaaaa cagaaaaagc
     9361 accgatagca acagaattct acgtttaatc ttagaaagta gcatacacgc tacaattagc
     9421 ataacaaact gtgtaaaagg ttttattaga aaatcatata gatatacttc taaattactc
     9481 gtaaaaagtt caccagcctc aaatctcatc atccgacctt cgcccgctcc atatagttca
     9541 ataactagag caaacttcat tgcgtaataa taagtaacaa tacatgccaa cataagaagt
     9601 aatatcatct tattattgcc aataaccaga ttataaaaac tattatctgt tgtaaccttc
     9661 aatcgtattt tttttatttt tccaaaacaa agaaatcccc aattaaacgc ccaaacattg
     9721 atcaataata acatatatgt tttcattgac acagaatata gttcagctgg atcaaaagta
     9781 gaaataacaa tagcagttcc ccaccacaat aaatatatga aacttatttt tcttgaaaaa
     9841 gaatcaatta taagcctatt agatatcaaa atctgaatta agaatagaat aagaaaaatt
     9901 atagcaatca tttgattcta tacttttgtc cctttgtcaa ccaccagaaa gaaacatata
     9961 aagccttata gatatctttc agattcttag gaaaataaga aaaagaatat ttcacaaata
    10021 aagtaaaatg atatatatat ttctttaagg aaatgtcaag tgattctgaa aatatagctc
    10081 tatgaacaaa tgccaaatat ctgtaataag gaaatttcaa atctttatta tgaaaatatt
    10141 caggaacttt taggtctaat acatgtaatg aataagaaac tctagaagta actcccattg
    10201 cattcttcct ataacaacac attacatctg gcatatataa aatatttcct ttcaacgaac
    10261 aagccaaaaa cagaaaacga tctccagcat ataaactact attgaaatct ttaatataat
    10321 caagtacttc ttttttaaaa aggaaagacg ctgtatgaaa aagtcttaca tccaataagt
    10381 ctttagtttt taaaatctgt tttttcacat gatgaaacat atgggatgga gttccattct
    10441 cataaaaaac taaagattga tgcgcacaac ctgctaacga atgatcactc tccattgcat
    10501 ttacttgttt ttgtaacttc aacggatcag tccaataatc atccccctca caaagcgcaa
    10561 catatttacc ttttactaaa ggaaataata tttgacgtaa cggagaaata ccttgagaat
    10621 actggtttcg aatctgaaga attggtttaa taagattagg atatgccaca acatattcat
    10681 taataatact ctgagtagca tcagaagacg catcatcatg aacaagaatt tcaaaatcaa
    10741 attctgtttt ttgcattata aggccatcta aacactgcct tatatatttt tcatgattat
    10801 acgtcaagca acatatgcta actgttattg aaccgttcat ttattttaat cttaattcag
    10861 caaaacgctt tatttccatt tcacttttat aatcctcaaa caacttacga caatcatatt
    10921 gaggcacata tcctaattct ttctttgcat tttcaatgtt cattatgtac ccaccaccac
    10981 aaactttgtc tggtctatat ataacttcag atggtgactc cttaggcgaa aaaacactaa
    11041 taatagtgcg aatttgttct tctaatgtaa ttggcattcc tgtacccaca ttataaaagc
    11101 cactttcttg ttccacctca acagctttac acaacatttg actaaaatcg ttaacatgaa
    11161 ccatatcctt tgcataatga ggatctcccc atatctctat aggtagtcct ttctgagcat
    11221 tttcaatcat ttggtaaata ggtcgcttcg tcttgactcc attaggaaag tagtacggat
    11281 aagggctata gctatatatt gttggaagcc taaaaatgaa ttttttcaaa ccgtattctt
    11341 gataataatg ctctatcaag tctaaagcag tatttttgga aattacatat atagcatgat
    11401 cacccgtata gctaaaattt ggtttcatat cgggttttaa aatagtatca tgagaataca
    11461 atgaaatatc aaaaacggtt tgagtataaa ttatcctatc tacatgacat ttgcggcaat
    11521 actctaatac attaaatcct cctattacaa tagaatctaa atactttatg gggcaataag
    11581 tatccatata agaaggtatt tgagcagaga gtagcataac tgcatgtata ttttccattg
    11641 gaagcttatc aaaatcttct tccttcgtaa tgtccacaga ataatatggt attccccttt
    11701 tggaaaaaaa agtagtttcg cgacgtcctg aagctattat ttcatactct tccttattaa
    11761 agaaatttaa tgcgtattct aacaaataag accctgtatt accggttgct ccaaaaataa
    11821 taattttctt tttcatgata aatggttatt taagttagat attttttcaa ttaatatatt
    11881 cattgttttt tcatcatatc tctgatctat aggcaatgca atcatattat tcgcgattga
    11941 gtattccaga catttagcgt ctatcttatt cactacgttt tgccaatatt gaggtacata
    12001 tacgtgctct aatattaatt gttggcgaag cccagctttg ctcgtataaa aaggatatat
    12061 caacgggaca ttttgatttt cagaaatact aaaatcaaat ttattaaatg cagttaatgc
    12121 actgtagaga agatgataat taaaacgacg cctttcaatt attgagacgt aatccaaaga
    12181 agacaaaatt ttatcagaca atacagacat tctatgtatc tcttgatttg acatcctttc
    12241 ttcagattct tgaaaatctt tataataccc ctctgcagag tcagttatac gccctatcaa
    12301 gtagttaata ttatcaatag gattataaat cgggaaatca atatccagta acgcagttgt
    12361 gtacaaataa gcgccatctg gaactccaaa aaatttacgg gcagaataaa atgtatctac
    12421 cccttcaatt ggttttgcaa aaaaagcttg tgcattatca actattaagt taggataaaa
    12481 ccgagataaa taaagaacag tatcttgttt taagccgaaa taattagtat atagaaaagc
    12541 ctctgagctt tctatttttt tatcaaacac aggatccagt aaactattta tatgataata
    12601 ttcatacttt acatttaatt tcatgagagg ttctattatc acatcacaag tgtaatatgg
    12661 aatatacact tttgtatacc gtctagcttt taatatatac tcaaagcatt gacgagcagt
    12721 actcagtttt attgcacctt gatgaaaatg ttctccttca cgtatttcca gttcaaaata
    12781 gccacctatt gacatcattg taaactaaat gaatctaata acatacaagt ttcttcttta
    12841 gaattattca ttagtacatc tattattgat aaatatggaa caaattcttt agattgctgg
    12901 ggataccttt ttattatcgt atctacaaat ttcaaattaa taccattttg cataaagtct
    12961 tccttattat agatttgttt tccacctata gcattataat aagtatctgc cgatagtaac
    13021 ttacaaatat gaagaacttt atcctttcct tttaaagagc aatctttagg taaatctgaa
    13081 gatagcaata tttcagtctg aatatttaga taatctaaaa tttctttaaa actattagcc
    13141 aagaagagag ccaattgtct atttggaaac ataattatcc tatacaacaa ttccattgtt
    13201 tcatgataga aaggagcttt agagtaattc aattccaatg ttttcataaa tttacgaaaa
    13261 tcatctccta tctctatttc attgaaatgt ttataggcac tagccccctt caaagtgata
    13321 gtaaacattt tcttttcacc atttaccaat atattgttac gattcaccca ccctccttta
    13381 atataattaa catcatcgta tacaacgtat ttatcaacag ccttaattag ctggaaataa
    13441 cctatataag gcataaaata aggttgcatt atacctaatt tcatttcaat atcagattta
    13501 aaattctttc aacatcttca ttcgacaatc cacaatacat tggcaagcag attacacttt
    13561 ttgccattt
//
