LOCUS       c00140_gnlPro..         5748 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MFJLCDGJ_140.
ACCESSION   c00140_gnlPro..
VERSION     c00140_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 05:06:35
            Original ID  :: gnl|Prokka|MFJLCDGJ_140
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5748
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5748
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3028:5746]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3029..5746
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..5748
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5748
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             41..1567
                     /locus_tag="ctg140_1"
                     /transl_table=11
                     /translation="MRTKRAFKEFCVLGGLASSVCGVAQERPNIIVFLVDDMGLMDTSV
                     PFIADESGQPVRHPLNDWYHTPNMERLAKQGICFSTFYAQSVSSPSRASIMTGQNATRH
                     GVTNWINAESNNRNPFGPPQWNWKGLRKDMPTMPRVLQQAGYKTIHVGKAHFGCMGSEG
                     ENPLNIGFDVNIAGSGIGHPGSYYGEWGYGHIKGQKIRAVPDLEKYHGTDTFLSEALTI
                     EANREITKAVEEKRPFYLNMAHYAVHSPFQADKRFLSRYTDPDKNEQARAFATLIEGMD
                     KSLGDIMDQLEKLGIAENTLILFLGDNGGDAPLGDERGYGSSAPLRGKKGTEFEGGMRV
                     PFIAAWAKPEKKSKVQKNLPIGVGSMQTQLGTIMDIYPTVLSVAGCEVPQNYVIDGFDL
                     KKQLSGKVDKKRPESFLMHFPHAHRGSYFTTYRMGDWKLIYYYLPETPKQPKALLYNLK
                     DDPEERNELSAAHPDQCREMIREMSARLEKEGALYPVDKQGNELKPFVYF"
     CDS             complement(1639..2868)
                     /locus_tag="ctg140_2"
                     /transl_table=11
                     /translation="MNKLLLIWIVVLLTSCITNQENARDTLFVNLTDATDSQSLKLSDF
                     GNTVRYVPLETNEVCLIGNNPHIALLEDKIVIATKDQCFLFHKQTGKYICSIGHIGDDP
                     SGYSSTNYWIDDAGLFYFFRAPDQLLKYNQKGEMTGKIQIPHIPAAPDFFAFSDSTIIA
                     HCNSSIGLESSNSLLFLNSFGEKLDSIPNLLKSPSIPSPDNISSLSIIKRGAGFFGNLG
                     RRGVMIIKDKEQGELLLPLYNPSLWSSENEVRFRETFTDTIYTIKNRKLYPYMVFHTDT
                     DHSSANPLWYSNPQSIYVAYVLENTHSVFFQYVKNKQVYNGLYNKETQQTKFAKCQQFI
                     VDDLTSEQELKVDLSALCSYKGEYGFILEAASIPDASQKQSDNKNENIPHWMSQLDEDA
                     NPVIAIVSDK"
     CDS             complement(3029..3784)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer4_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg140_3"
                     /sec_met_domain="Fer4_7 (E-value: 2.9e-13, bitscore: 38.5,
                     seeds: 208, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_3 (E-value: 2.1e-26, bitscore: 80.3,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.3e-07, bitscore: 20.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKNISFTLKVWRQAGPKAKGAFETYQMKDIPGDTSFLEMLDILN
                     EQLISERKEPVVFDHDCREGICGMCSLYINGHPHGPATGATTCQIYMRRFNDGDTITVE
                     PWRSAGFPVIKDLMVDRTAYDKIMQAGGYVSVRTGAPQDANAILIAKPIADEAMDAASC
                     IGCGACVAACKNGSAMLFVSAKVSQLNLLPQGKPEALRRAKAMLSKMDELGFGNCTNTR
                     ACEAECPKNISISNIARLNRDFIIAKLKD"
     CDS             complement(3821..5746)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 200.7; E-value:
                     1.4e-61)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg140_4"
                     /sec_met_domain="FAD_binding_2 (E-value: 9.1e-63, bitscore:
                     200.7, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 4.3e-38,
                     bitscore: 118.4, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="NYKAHQKLVNPANKRRLDIIVVGTGLAGASAAASLGEMGFRVFNF
                     CIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTVKGGDYRAREANVYRLAEVSN
                     AIIDQCVAQGVPFAREYGGTLDNRSFGGAQVSRTFYAKGQTGQQLLLGAYSALSRQVNV
                     GTVKLFTRYEMQDVVIIDGRARGIIAKNLITGELERFAAHAVVIATGGYGNAYFLSTNA
                     MGCNCTAAISCYRKGAVFANPAYVQIHPTCIPVHGDKQSKLTLMSESLRNDGRIWVPKK
                     KEDAIKLQKGEIKGSDIPEEDRDYYLERRYPAFGNLVPRDVASRAAKERCDAGFGVNNT
                     GLAVFLDFSEAINRLGIDVVLQRYGNLFDMYEEITDVNPGELAKEISGVKYYNPMMIYP
                     AIHYTMGGIWVDYELQTTIKGLFAIGECNFSDHGANRLGASALMQGLADGYFVLPYTIQ
                     NYLADQITVPRFSTDLPEFAEAEKAVQAKIDKFMSIQGKESVDSIHKKLGHIMWEYVGM
                     GRTAEGLKKGIAELKAVRKEFETNLFIPGSKEGMNVELDKAIRLYDFITMGELVAYDAL
                     NRNESCGGHFREEYQTEEGEAKRDDENFFYVACWEYQGDDEKAPVLHKEPLVYEAIKVQ
                     TRNYKS"
ORIGIN
        1 cacgtacaat aactaggata taaatcataa atactatgta atgagaacaa aacgagcttt
       61 taaggaattt tgtgtattgg gtggtttggc atcttccgtt tgcggagtag ctcaggagcg
      121 cccgaatatt attgttttcc ttgtagatga catgggactt atggatacgt cggttccttt
      181 tatcgcagat gagagtggtc agccggtgcg tcatccgctg aatgactggt atcatacccc
      241 gaatatggag agactggcta aacagggtat ttgtttctct actttttacg cacagagcgt
      301 gagttcccct tccagagctt ccatcatgac cggacagaat gcgaccaggc atggagtcac
      361 taactggatt aatgctgaga gcaacaaccg gaatcctttc ggaccgcccc aatggaactg
      421 gaaaggtctc agaaaagata tgcctaccat gccgagagtc ctgcaacaag ccggatataa
      481 aaccattcat gtaggaaagg cacatttcgg ctgtatggga agcgaaggag agaatccgct
      541 gaatatcggc tttgatgtaa atatcgcagg ttccggcatc ggccatcccg gcagctatta
      601 tggcgaatgg ggatacgggc atatcaaggg acagaagatt agagcagttc ctgacctgga
      661 gaaatatcac ggtacagata ccttcttgag tgaagcgctg actatagaag cgaatcggga
      721 gatcaccaaa gccgtagaag aaaaacgacc tttctatctg aatatggccc attacgcagt
      781 tcattcacct ttccaggcag acaaacgttt cttgtcacgc tacaccgacc cggataagaa
      841 cgagcaggca agagcttttg ctacattgat agaaggcatg gataaatcat taggcgacat
      901 catggaccaa ctcgaaaagc tgggaattgc cgaaaatacg ctgattctct ttttaggaga
      961 taacggtggt gatgctccat tgggagatga acgtggctat ggttcttccg ctccgctgag
     1021 aggtaaaaag gggacggagt ttgaaggtgg tatgcgtgtt cctttcatcg cagcttgggc
     1081 aaagcctgag aagaagagca aagtacagaa gaacttgcct attggagtag gaagtatgca
     1141 gacacagttg ggcacaatca tggatatcta tccgactgtt ctgtctgtgg caggatgcga
     1201 agttccccaa aactacgtga tagacggatt cgatctgaaa aagcaactga gcggtaaggt
     1261 ggacaagaag cgtcccgagt ctttcctgat gcacttccct cacgcacacc gcggcagcta
     1321 cttcaccacc tatcgcatgg gcgactggaa actgatttat tattatcttc ccgagactcc
     1381 gaaacaaccc aaagccttgc tttacaatct gaaagacgat ccggaagaaa ggaatgagct
     1441 gtctgccgca catccggatc aatgcagaga gatgattcgg gaaatgtccg cccggctaga
     1501 aaaggaagga gctctttatc cggtggataa acaaggaaat gagttgaagc cttttgttta
     1561 cttttaaata ggtgctacta tttcaactgt ctgttttgcc aaagcatatc tattaggcaa
     1621 aacagacaga attatatatc atttgtcaga tacaattgct atgaccggat tggcatcttc
     1681 atccaattgg ctcatccaat ggggaatatt ctcattttta ttgtcagatt gtttttgaga
     1741 agcatcagga atactggcag cttccagaat aaatccatat tctcctttat aactgcacaa
     1801 tgccgacagg tcaactttca gttcctgctc tgaagtgaga tcgtcaacaa tgaattgctg
     1861 gcactttgca aacttagttt gctgtgtttc tttattatac aaaccattat agacttgttt
     1921 gttttttacg tattgaaaaa atacagaatg tgtgttttcc aatacataag ctacgtagat
     1981 actttgcgga ttactatacc ataaaggatt ggcggatgaa tgatctgtat ctgtatgaaa
     2041 aaccatatat ggatatagtt tcctgttttt aattgtgtat atcgtgtctg tgaaggtttc
     2101 tctgaaacgt acttcattct cggacgacca taggctggga ttatagagag gcagcagtaa
     2161 ttctccttgc tccttatctt tgataatcat tacccctctt cttcccagat ttccaaagaa
     2221 acctgctcct ctttttatga tagaaaggct ggatatatta tccggagaag ggatagaggg
     2281 cgattttaat aaattgggaa ttgaatccag cttttctcca aaggagttga ggaaaagcaa
     2341 agagttgctg gattccaatc ctatactact attacaatgg gcaataatgg tagagtcgga
     2401 aaacgcgaaa aagtcgggtg cggcaggtat atgtggaatt tgaattttcc cggtcatttc
     2461 tcctttctga ttatatttta gcaactggtc cggtgcgcga aagaagtaaa aaagacctgc
     2521 atcatctatc cagtaatttg tcgagctata tccacttgga tcatcaccga tatggcctat
     2581 actgcatata tacttcccgg tctgtttgtg gaataagaaa cattggtctt tagtagcaat
     2641 cacaatcttg tcttccagca gcgctatatg gggattgttt cctatcaggc atacttcgtt
     2701 ggtttccaaa ggtacataac gtacagtatt tccgaagtca gataacttta ggctttggga
     2761 atcagttgca tccgtaagat ttacaaataa tgtatccctg gcattctcct gatttgtgat
     2821 gcatgaggtt agtaagacta ctatccatat cagcaataat ttattcatcg tatttaattt
     2881 atttgattgt ctggccttat gtcacaaaga aaagaataaa aaacggataa tgaacaagct
     2941 atctataaat aattggtcag taaaaagaaa tcccccgcca gctaacgccg acgggggatt
     3001 tatgctatta atcaaatcaa gctcacgctt agtctttcag tttagcgatg atgaagtcgc
     3061 ggttcaggcg ggcgatgttg ctgatagaga tgttcttcgg acattcagcc tcgcaagcgc
     3121 gggtgttggt acagttaccg aatccgagtt cgtccatctt ggacaacata gctttcgcac
     3181 ggcgcagagc ttccggttta ccttgcggaa gcaagttcaa ctggctgacc tttgccgaaa
     3241 cgaacaacat ggcagaacca ttcttacaag cagctacaca agcaccgcaa ccgatacaag
     3301 aagcggcatc catagcttcg tcagcgatag gcttcgcgat caggatagcg ttagcatcct
     3361 gcggagcacc tgtgcgtacg cttacataac caccagcctg cataatcttg tcgtatgccg
     3421 tacggtctac catcaagtcc ttgataaccg ggaaaccggc cgaacgccaa ggctcaacag
     3481 tgatggtgtc accatcgttg aaacgacgca tatagatttg gcaagtagta gcacctgttg
     3541 caggaccgtg cgggtgtccg ttgatgtaaa gagaacacat accgcagata ccttcgcggc
     3601 agtcatggtc gaatactacc ggttcttttc tttcgctgat gagctgttcg ttcagaatat
     3661 ccagcatttc gaggaaggaa gtatcaccgg ggatatcttt catttggtag gtttcaaaag
     3721 cacctttagc tttcggacca gcttggcgcc ataccttcag tgtaaatgat atatttttat
     3781 ccattttctt ctaattcttt taaatgttta acttcaccga ttagctcttg tagttacgag
     3841 tctgaacctt gatagcttcg tataccagcg gttctttgtg caataccgga gccttttcat
     3901 cgtcaccttg atattcccag catgctacat agaagaagtt ttcgtcatca cgttttgctt
     3961 ctccttcttc agtctggtat tcttcacgga agtgaccacc acaactttcg ttacggttca
     4021 atgcgtcgta agcaacgagc tcgcccatag tgatgaagtc atacagacgg atagccttgt
     4081 cgagctctac gttcatacct tccttagaac cggggataaa cagattggtt tcgaattcct
     4141 tgcggactgc tttcagttcg gcgatacctt tcttcaggcc ttctgccgta cgtcccatac
     4201 ctacgtattc ccacatgatg tgacccagtt tcttgtggat agaatcaact gattctttac
     4261 cctggatgct catgaacttg tcgatcttag cctgaactgc cttttctgct tcggcaaatt
     4321 ccggaagatc ggtagagaaa cgaggaacag taatctgatc tgccagatag ttctggatag
     4381 tgtaaggcaa tacgaagtaa ccgtcggcca gaccttgcat caatgcggaa gcaccgaggc
     4441 ggtttgcacc gtggtcggag aagttacatt caccgatagc gaacagaccc ttgatagtgg
     4501 tttgcagttc gtagtctacc cagataccac ccattgtgta gtggatagcc ggataaatca
     4561 tcatcggatt ataatatttc acaccactga tttcttttgc cagttcgccc ggattaacgt
     4621 cagtgatttc ttcatacatg tcgaagaggt taccataacg ctgaagcact acatcgatgc
     4681 ccaggcggtt gatagcttca gagaaatcga ggaatacggc cagacctgtg ttgttcacac
     4741 cgaaacctgc gtcgcaacgt tctttagctg cacggctggc aacgtcacgc ggaaccaggt
     4801 taccgaatgc cggataacgg cgttccaggt agtagtcgcg gtcttcttca ggaatatcgc
     4861 ttcccttgat ttcgcctttc tgcaatttga tagcgtcttc tttcttcttc ggaacccaga
     4921 tacgaccgtc gttacgcaga gattcggaca tcagagtcag tttggactgc ttgtcaccgt
     4981 gtaccgggat acaagtcggg tgaatctgaa cgtatgcggg gttagcgaat acagcaccct
     5041 tgcggtagca agagatagca gctgtacagt tacaacccat agcgttggta gacaggaagt
     5101 aagcgttacc gtaaccaccg gtagcgatta ctacggcgtg agcagcgaaa cgttccagtt
     5161 cgccggtgat taagttcttg gcgatgatac cgcgagcacg tccgtcgatg atcacaacgt
     5221 cctgcatttc ataacgggtg aacagcttca ccgtacctac gtttacctga cggcttagtg
     5281 cggagtaagc acccagcaac agctgctgac cggtctggcc tttagcgtag aacgtacgtg
     5341 atacctgtgc accaccgaaa gaacggttgt ccagtgtacc gccgtattcg cgtgcaaaag
     5401 gaacaccctg ggctacgcat tggtcgatga tagcgttgga tacttcggcc agacggtaaa
     5461 cgttggcttc acgtgcacga tagtcaccac cttttactgt atcgtaaaac agacggtaaa
     5521 cggagtcacc atcattctga taattctttg cagcattgat accaccctgt gcagcgatag
     5581 agtgtgcacg gcgcggagag tcctgaatac agaagttgaa tactctgaaa cccatttcac
     5641 cgagggatgc agcagcagaa gcacctgcca gacccgtacc cacaacgatg atatccaaac
     5701 ggcgtttatt agcggggttc accaatttct ggtgagcttt atagttgg
//
