LOCUS gnlProkkaFNOABGIA_196 3941 bp DNA linear UNK 01-JAN-1980 DEFINITION gnl|Prokka|FNOABGIA_196. ACCESSION gnlProkkaFNOABGIA_196 VERSION gnlProkkaFNOABGIA_196 KEYWORDS . SOURCE ORGANISM . COMMENT ##antiSMASH-Data-START## Version :: 7.1.0 Run date :: 2024-11-17 23:46:51 Original ID :: gnl|Prokka|FNOABGIA_196 NOTE: This is a single region extracted from a larger record! Orig. start :: 0 Orig. end :: 3941 ##antiSMASH-Data-END## FEATURES Location/Qualifiers CDS 1..1008 /gene_functions="biosynthetic-additional (smcogs) SMCOG1025:diguanylate cyclase (Score: 141.8; E-value: 6.8e-43)" /gene_kind="biosynthetic-additional" /locus_tag="ctg196_1" /transl_table=11 /translation="MYELSYKYLEQAERQLDEEVEEMYHYTVLIFKCRLLWQMGKGDYV YEYMEDLLASGEKDSNASDYLNDMKDLCQLFRDMKEYEYWKQTISAVETYTIDQKTVFF QLFLTELWMDYYQTVGNEERYVELCVAHADLYKRQKAIDVKDRATAIDIKVQLREKEAE RKRVETMSVTDALTGLGNRYSLEREAVHIIRDAGGERITVGVLDIDCFKQLNDTYGHIQ GDRCLKVVADILKEAVMDCGHVYRFGGDEFVMLFPAGMENRIEEIAEAILQKIAEAGIA NEHSVVQPNLTISQGYACFVPEGTESGARLIEHADKALYYVKRNSRNAYYIIRE" protocluster 1..3941 /aStool="rule-based-clusters" /category="RiPP" /contig_edge="True" /core_location="[2028:2286]" /cutoff="10000" /detection_rule="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /neighbourhood="10000" /product="RRE-containing" /protocluster_number="1" /tool="antismash" proto_core 2029..2286 /aStool="rule-based-clusters" /tool="antismash" /cutoff="10000" /detection_rule="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /neighbourhood="10000" /product="RRE-containing" /protocluster_number="1" cand_cluster 1..3941 /candidate_cluster_number="1" /contig_edge="True" /detection_rules="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /kind="single" /product="RRE-containing" /protoclusters="1" /tool="antismash" region 1..3941 /candidate_cluster_numbers="1" /contig_edge="True" /product="RRE-containing" /region_number="1" /rules="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /tool="antismash" CDS 1106..1903 /gene_functions="regulatory (smcogs) SMCOG1008:response regulator (Score: 89.4; E-value: 4.1e-27)" /gene_kind="regulatory" /locus_tag="ctg196_2" /transl_table=11 /translation="MEKLNVAIADDNERMLRLLGDIIESDDELNVVGVARDGEEAYHVI KAKEPDVVLLDIVMPKLDGLGVLDRVSQDTSIKKHPAFIMISAIGQEKITEDAFARGAD YYIMKPFDNDIVLSRIKNVRRPAAGRGAEVRKVNAYEKAEETKERNLEADVTDIIHEIG VPAHIKGYQYLRDAIVMSVNDMDMLNSITKILYPTIAKKYQTTSSRVERAIRHAIEVAW SRGKMDTIDEMFGYTIHNGKGKPTNSEFIALITDRIRLEYKMH" CDS 2029..2286 /gene_functions="biosynthetic (rule-based-clusters) RRE-containing: Stand_Alone_Lasso_RRE" /gene_functions="biosynthetic-additional (rule-based-clusters) PF05402" /gene_kind="biosynthetic" /locus_tag="ctg196_3" /sec_met_domain="PF05402 (E-value: 1.7e-13, bitscore: 38.9, seeds: 461, tool: rule-based-clusters)" /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 1.1e-10, bitscore: 29.6, seeds: 25, tool: rule-based-clusters)" /transl_table=11 /translation="MQKNRYQLRCAAGKYWLLDMEQDGETYRPPLMLNESGAYIFRQYM AGNTADKIAEELATMYGGTADDMRTDVMQFIRQMELQGIL" CDS 2290..3939 /gene_functions="biosynthetic-additional (rule-based-clusters) RmlD_sub_bind" /gene_functions="biosynthetic-additional (smcogs) SMCOG1010:NAD-dependent epimerase/dehydratase (Score: 63.9; E-value: 2.8e-19)" /gene_kind="biosynthetic-additional" /locus_tag="ctg196_4" /sec_met_domain="RmlD_sub_bind (E-value: 1.7e-14, bitscore: 41.7, seeds: 37, tool: rule-based-clusters)" /transl_table=11 /translation="MNILLVGSKCRFLQLLTDKLDKEGHRVFLLTAEDKSVRTSKKIFE TYHFAYDNPCIREVLEGVRPDVTVFMGAYDTGFLWGKGSSTASAYTSGLFQLLMNETAG NVGRFLYLSSEEVFGGEYTQDISCEENCAAYTVRAQAIAAGEELCRSYFNMGYETIVLR LDHLYGEPLTGAEARDICARMSVEGLETGEIRVNPHNRVALLHYSDAVEFLYAVITAKK MTYGVYQISSGETISVRSAAEFVAAELEGVKVVEDTGRERMEPVLSNQRFAEEFGIRIV HKPETEIGREAAYIRQHASSFVRTQSRKKGWSRFGERSREAVRAMIPFAENLLCFLPFF LLNNWMAGSAYFQNIDFYLLYVLLFAITYGQQQATFSSILAVAGYLAVQMYQRSGFDVV LDVNTYIWIAQLLILGLVIGYMRDRLHAVEEEERHEVDFLSRQVEDISDVNGSNVRIKE ILSNQIVNQNDSFGKLYEITSSLDKYEPSEVLFYAAEVLAKLMDSQDVAIYTVANHSYA RLFSATSPKARMLGNSIHYVQMEELYEKLREKK" ORIGIN 1 ctgtacgagc tttcctacaa atatttagag caggcagagc ggcagcttga cgaggaagtg 61 gaggagatgt atcattatac ggtactgatc ttcaaatgta ggctgctctg gcagatggga 121 aagggggact atgtgtatga gtacatggag gatcttctgg cgagcggtga aaaagacagc 181 aatgcgtcgg attatctgaa cgatatgaag gatctgtgtc agctgttccg cgacatgaaa 241 gagtatgaat actggaagca gaccatatct gcggtcgaga cctatacgat cgatcagaaa 301 accgtctttt tccagctgtt tctgacggaa ttgtggatgg actactatca gactgtgggg 361 aacgaagagc gctatgtgga actgtgtgtg gcacatgcgg atctgtataa gcgccagaaa 421 gcaatcgatg tgaaggatcg tgcaacagcg atcgacatca aggtccagct gcgggaaaaa 481 gaggcggagc gcaagcgcgt ggagaccatg tctgtgacgg atgcgctgac cggactgggg 541 aaccgctatt ctcttgagcg tgaggcggtg cacatcataa gggatgcggg cggtgagcgg 601 atcacggtcg gcgttttgga catcgactgc tttaagcagc tgaacgatac ctacggacat 661 attcagggag accgctgcct gaaggtggtg gcggatattc tgaaggaggc agtgatggac 721 tgcggacatg tctaccggtt tggcggggat gagttcgtga tgttgtttcc ggcagggatg 781 gagaaccgga tcgaggagat cgcggaggcg atcctgcaga agattgcgga ggcgggaatc 841 gcaaacgagc actcggttgt gcagccgaat cttaccatct cccaggggta tgcctgcttt 901 gtgccggagg gcacagagag cggagcacgt ctgattgagc acgcggacaa ggcgctttac 961 tatgtgaaga gaaacagcag aaacgcatat tatatcatac gcgaatgatg catatacttc 1021 agttgacgac tagcttcgac aaattttctt gcgttggaat cagtatcatc agtaagcaaa 1081 gagttttgtc acaggagggg ctaaaatgga aaaactgaat gttgcaatag cagatgataa 1141 tgagagaatg ctgcgattat taggggatat tatcgaaagt gatgacgaac tgaatgttgt 1201 aggagtcgcc cgcgacggcg aggaagcata ccatgtgata aaggcgaaag aaccggatgt 1261 cgtgctgctc gacattgtga tgccgaagct tgacggattg ggggttttag accgcgtgag 1321 tcaggacacc tccataaaaa agcatccggc gtttattatg atcagcgcga tcggtcagga 1381 gaaaatcacg gaagatgcgt ttgcgcgcgg cgcagattat tacattatga agccgtttga 1441 caatgatatc gtgttaagcc gcatcaagaa tgtgagaaga cctgcggcgg gacgcggtgc 1501 ggaagtgcgc aaggtcaatg cctatgagaa ggcggaggag acaaaggagc gcaacctgga 1561 agcggatgtc accgatatta ttcacgagat cggagttccg gcgcatatca aagggtatca 1621 gtatctgcgg gatgcgattg tcatgtccgt caatgacatg gatatgctca actcgatcac 1681 gaaaattctt tatcccacga tcgcgaagaa gtaccagaca acctccagcc gtgtggagcg 1741 ggcaatccgg catgcgatcg aggtggcgtg gagcagaggc aagatggata ccattgatga 1801 gatgttcggg tataccatcc acaacggaaa aggaaaaccg acgaattcgg agtttatcgc 1861 actgattacc gaccggatac gtctggaata taagatgcat taacgatgag gacatatttt 1921 tcaggaggcg caaagccgaa gaagagtatg tcctcatttt cggataaccg gttccttttg 1981 agcgtcagat gtgctataat tatacgatga tttttcggag gatggcagat gcagaaaaac 2041 aggtatcagc tcaggtgcgc ggcaggaaaa tactggctgt tagatatgga gcaggatgga 2101 gaaacgtatc ggccgccgct catgttaaat gagagcgggg cttatatctt caggcagtat 2161 atggcaggaa acacggcgga taaaatcgcg gaggagcttg ccactatgta cggggggacc 2221 gcagacgata tgcggacaga tgtgatgcag tttatcagac aaatggaact acaggggatc 2281 ttgtaaacga tgaatatatt attggtggga agcaaatgcc gcttccttca gcttttgacg 2341 gacaaactgg acaaagaagg gcaccgtgtg tttttgctga cggcagaaga caaatcggtc 2401 agaacgtcca aaaagatatt tgagacgtat cattttgcat acgacaatcc gtgcatcaga 2461 gaggtgcttg agggagtgcg tccggatgtg acagtcttta tgggcgccta tgatacaggc 2521 tttctctggg ggaaggggag cagcacggcg tcggcgtaca cttcaggcct gtttcagctg 2581 ctgatgaatg agacggctgg taatgtggga cgctttttgt atctctcgtc ggaggaagtc 2641 ttcgggggag agtatacaca ggacatttcg tgcgaggaaa actgtgcggc ttatacggta 2701 cgcgcgcagg cgattgcggc gggagaggaa ctctgccgca gttactttaa catgggctat 2761 gagacgatcg tgctgcggct ggatcatctc tacggggaac cgcttaccgg ggcggaagcg 2821 cgtgatatct gcgcccgcat gagtgtggag gggctggaaa ccggtgagat ccgggtaaat 2881 ccccacaacc gtgtggcgct gttacactat tcggatgccg tcgaattttt gtatgcggtg 2941 attacggcaa aaaagatgac gtacggcgtc tatcagattt cttccggaga gacgatttcc 3001 gtgcgaagcg cagcggagtt tgtggcggcg gaactggaag gtgtgaaagt cgtagaggac 3061 accggaagag agcggatgga gccggttttg tccaaccaga gatttgcgga ggagtttggg 3121 atacgcattg tacataagcc ggagaccgag attggcaggg aggcggcgta catccggcag 3181 cacgcatctt cttttgtgcg gacacagagc agaaaaaagg gatggagccg gttcggagag 3241 cggtcgagag aggccgtaag agcgatgatt ccgttcgcgg agaatctgct ttgtttcctt 3301 ccgtttttcc tgttaaataa ctggatggcg gggagcgcgt atttccagaa cattgatttt 3361 taccttctgt atgtgctgct gttcgccatt acctacgggc agcagcaggc gacattctcc 3421 tcgattcttg cggtggcggg gtatcttgcg gtgcagatgt atcagcggag cggttttgat 3481 gtggtgctgg atgtcaacac gtacatatgg attgcacagc tgctgattct gggacttgtg 3541 atcggctata tgcgcgaccg gctgcatgcg gtggaggagg aggagcgcca tgaggtggat 3601 ttcctctcca gacaggtgga ggacatctcg gatgtcaacg gcagcaacgt gcgcatcaag 3661 gagattctgt caaatcagat cgtcaaccag aacgacagct tcggaaagct gtatgagatc 3721 acgtccagcc tggacaagta cgagccgagt gaagtgttgt tctatgccgc agaggtgctg 3781 gcaaagctga tggacagtca ggatgtcgca atctatacgg tggcaaacca ctcctatgcg 3841 cgtctgttct cggcaacttc gccgaaggca cgcatgctcg gaaattccat tcactatgtg 3901 cagatggaag aactctatga aaagctgcgg gagaaaaaag t //