LOCUS       c00127_gnlPro..         6689 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FNOABGIA_127.
ACCESSION   c00127_gnlPro..
VERSION     c00127_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 01:31:15
            Original ID  :: gnl|Prokka|FNOABGIA_127
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6689
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6689
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[48:5336]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      49..5336
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..6689
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6689
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(49..1467)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: HMGL-like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: PYC_OADA"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg127_1"
                     /sec_met_domain="HMGL-like (E-value: 4.6e-46, bitscore:
                     146.1, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="PYC_OADA (E-value: 5.2e-60, bitscore:
                     191.3, seeds: 434, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEVVKKPLKITETVLRDAHQSLIATRMTTEQMLPIVDKMDKVGY
                     NAVECWGGATFDACLRFLKEDPWDRLRKLRDGFKNTKLQMLFRGQNILGYRPYADDVVE
                     YFVQKSIANGIDIIRIFDCLNDVRNLQTAVTACNKEKGHAQVALSYTLGDAYTLDYWME
                     MAKKVEDMGADSLCIKDMAGLLVPTKATELIQALKSATNLPIELHTHYTSGVASMTYMK
                     AVEAGCDIIDTAMSPFSMGTSQPATEVMVEAFKGTEFDTGLDQNLLAEIADYFRPMREE
                     ALESGLMNPKVLGVNIKTLLYQVPGGMLSNLLSQLKEQGKEDKFEEVLAEVPRVRKDLG
                     EPPLVTPSSQIVGTQAVFNVLMGERYKVATKETKDVLLGKYGQTVKPFNPEIVDKVLGE
                     EKKNAITCRPADLLEPELDKIEAEMKQWKQQDEDVLSYALFPQVATDFFKYREAQQKKV
                     DASVADTKNGAYPV"
     CDS             complement(1533..2681)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg127_2"
                     /sec_met_domain="OAD_beta (E-value: 1.3e-157, bitscore:
                     512.8, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYFSETMGNLLHQTAFFNLTWGNFVMIIVACFFLYLAIAKGFEP
                     LLLVPIAFGMLLVNIYPDIFADPEQMSNGVGGLFHYFYVLDEWSILPSLIFLGVGAMTD
                     FGPLIANPISFLLGAAAQFGIFGAYFLAIMMGFNDKAAAAVSIIGGADGPTSIFLAGKL
                     GQSGLMGPIAVAAYSYMALVPIIQPPIMKLFTTKKERAIKMENLRPVSKLEKILFPIVV
                     TVVVCTILPTTAPLVGMLMLGNLFRESGVVRQLTDTASNALMYIVVILLGTSVGASTSA
                     EAFLNTTTLKIVALGLIAFMFGTAAGVLFGKLLCFLTKGKINPLIGSAGVSAVPMAARV
                     SQKVGAEEDPTNFLLMHAMGPNVAGVIGTAVAAGVFMAIFGI"
     CDS             complement(2697..3062)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 61.7; E-value:
                     1.5e-19)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg127_3"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1.4e-20, bitscore:
                     61.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSYTITVNGNVYDVTVEENGAVSAPAAAPRRAAAPTAAPAAKPA
                     AAAGAGSVKIEAGAAGKVFKLEKSVGDAVKKGDSIMVLEIMKMETPVVATQDGTVASIN
                     VSVGDMVEAGALLATLN"
     CDS             complement(3104..3856)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg127_4"
                     /sec_met_domain="OAD_gamma (E-value: 2.4e-26, bitscore:
                     81.0, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQNRKVKFMKKKLSLILCLCFMALGLAACGTDPTTVDYFGMTYD
                     ELQSSMEQEVSALVGLTDDNRTYIQSYGSDTAMKLVESWDEATSDVGAYQGLGEFNITK
                     AQKTLTVDQYVEYQNREVIVSYIYTYNYETEQPELTDASADKVYTLGEKMEKAALNTLM
                     GMGTVFVVLILISLIIYCFRFISVIQNKAAGKGKKNEAEAGAVVEQIGQREEQLTDDLE
                     LVAVISAAVAASTGASADSFVVRSIRRR"
     CDS             complement(3843..5336)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg127_5"
                     /sec_met_domain="CT_Vparvula (E-value: 1.3e-125, bitscore:
                     408.5, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPHPKWMWTTIYRWRAKRMNSSNDSLAMQRITRLVDENSFIEIG
                     SLVTARSTDFNLAATDTPSDGVITGHGLIDGNLVFIYSQDASVLNGTIGEMHAKKIAAV
                     YDMAMKMGAPVIGFIDCAGVRLQESVDALDGFGLIYAKEVAASGVVPQISAVFGSCGGG
                     LAVVPALSDFAFIEESKGKMFVNSPNAIDGNRIEKCDTSSAAFQADENGCIDATGTEDE
                     ILADIRELVGMLPLNNEGDVYTDECADDLNRACESMDAMKGDPRYLLSEISDSHTFFET
                     KAGYARDMVIGFIKLNGMTVGAVANCTTVYDEEGKESEKFDNVLSAKGCEKAAEFVSFC
                     DAFEIPVLTLTNVKGYKACKCSEKRLAKALAHLTSAFAGATCPKVNLITGEAYGTAYVA
                     MNSKSIGADFVYAWPDAKVGMMDADLAVKIMYADASADELAEKAKEYDALQGSVMTAAR
                     RGYVDLIVDPADTRKYLVDAFELLYTKCAYTPDKKHGTK"
     CDS             complement(5465..6688)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S11"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg127_6"
                     /sec_met_domain="Peptidase_S11 (E-value: 1e-51, bitscore:
                     164.3, seeds: 11, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="FWENGMYRRFLSLLLSMSLLIFSPVRVRAQDVPAESELYAKSAVL
                     MDADSGRILYEKNGHEAMANASTTKILTCIVALENCDPASVVTVSKNAASQPKVHLGMH
                     EGQQFYLRDLLYGLMLESYNDCAVAIAEFAAGEVQGFAALMNAKAEELGCEDSYFITPN
                     GLDAKDDAGFHHTTAADLAKIMKYCIKESLKSDEFLEITRTAQYSFSDAEGKCTYQCTN
                     HNAFLQMMEGALSGKTGFTGTAGYCYVGALQREDKTLIVALLACGWPNNKTYKWSDTRK
                     LMNYGLEQFKKLNLLQIEPDEAELAPIEVRDGQGGQIGETVYLKPVVRNYAGEESILAP
                     LSSEVTLKYEIADRLYAPVKSGDYIGKIRYMLGEETLAERCVVAGETIGKIDYPWCLGQ
                     VLRLLWIE"
ORIGIN
        1 aaaaagcagg tgactgctca cccgcttttt gttataactg ttttttgatt ataccggata
       61 tgctccgttc ttggtatctg caacgcttgc atcgactttc ttctgctgtg cctcacggta
      121 cttgaagaaa tcggttgcaa cctgtgggaa cagtgcgtag gacaatacgt cctcgtcctg
      181 ctgcttccac tgcttcatct cagcctcgat cttatccagc tccggctcaa gcagatctgc
      241 cggacggcag gtaatggcgt tcttcttctc ctcgccaagt accttgtcta cgatctccgg
      301 gttgaacggt tttacagtct gaccgtactt tccaagcagt acgtctttgg tctccttggt
      361 tgccactttg tagcgctctc ccatcagtac gttgaacaca gcctgtgtac caacgatctg
      421 tgaggacggt gtaacaagtg gcggctcacc gaggtcttta cgcacacgcg gaacctctgc
      481 aagaacctcc tcgaacttgt cttccttgcc ctgctctttt aactgagaca gaaggttgga
      541 taacatacca cccggtacct gatataacag agtcttgatg ttaacgccga gaaccttcgg
      601 gttcatcaga ccgctctcta acgcttcctc acgcatcgga cggaagtagt cagcgatctc
      661 agctaacagg ttctgatcca gaccggtatc gaactctgtg cccttgaatg cctctaccat
      721 aacctcggtt gccggctggc tggttcccat ggagaacgga gacattgctg tatcgataat
      781 gtcacagcct gcctcaaccg ccttcatgta tgtcatggaa gcgacaccag aagtatagtg
      841 tgtatggagt tcgatcggaa ggttggttgc agactttaat gcctgaatca gctcggttgc
      901 cttcgtcgga accaaaagac ctgccatatc cttgatacat aaggaatcag cacccatatc
      961 ctctactttc ttcgccatct ccatccagta atccagagtg taggcatcgc caagtgtata
     1021 ggataatgcg acctgtgcat gtcctttctc tttgttacat gcggtaacgg ctgtctgcag
     1081 gttgcggaca tcgtttaagc agtcgaaaat acgaatgata tcaataccgt ttgcgatcga
     1141 tttctgtacg aaatactcta ccacatcatc cgcatacgga cgatatccta agatgttctg
     1201 tccacgaaac agcatctgaa gcttggtgtt cttgaatccg tctcttaact tgcggagacg
     1261 atcccacgga tcttctttca ggaaacgaag gcatgcatcg aaggtagcac cgccccagca
     1321 ctcaaccgca ttgtatccga ccttatccat cttgtctacg atcggaagca tctgctcggt
     1381 tgtcattctt gtcgcaatca gggactggtg cgcatcacgc agcaccgtct ctgttatttt
     1441 taaaggtttt ttaacaactt ctgacatttt ttcctcctta tcagcggata ctttgatacc
     1501 gctatccgtc tatgacggca tttgctcttt cactaaattc cgaaaattgc catgaataca
     1561 ccggctgcta ctgcagtacc gataacgccg gcaacgttcg gtcccatcgc atgcatcagc
     1621 aggaagttcg tcggatcttc ctctgcaccg actttctggg atacacgggc tgccatagga
     1681 acggcagata caccggcaga tccgatcaga ggattgatct tgccctttgt gaggaagcat
     1741 aacagcttac caaacaggac accggctgct gtaccgaaca taaatgcaat cagtccaagt
     1801 gcaacgatct ttaatgtcgt tgtatttaag aacgcctccg cactcgtaga tgcacctacg
     1861 gaagtaccga gcaggataac tacgatgtac atcaatgcgt tggatgcggt atcggtcagc
     1921 tggcggacca caccgctctc gcggaacagg ttaccgagca tcagcatacc aaccagcggc
     1981 gctgtcgtcg gaagaatcgt acatacaacc accgtaacca cgatcgggaa gagaatcttc
     2041 tccagcttgg aaaccggtct taagttctcc atcttgatgg cgcgctcttt tttggttgta
     2101 aacagcttca taattggcgg ctggatgatc ggcacaagcg ccatatagga atatgccgcc
     2161 acagcgatcg gtcccatcag tcccgactgt cctaactttc cggcaaggaa aatggaggtc
     2221 ggtccatcgg ctccaccgat gatggaaaca gcagctgctg ccttatcatt aaagcccatc
     2281 atgattgcaa ggaagtacgc tccgaaaata ccgaactgag ctgcagctcc aagcaggaaa
     2341 ctgatcgggt ttgcgatcag cggtccgaaa tctgtcatag caccgactcc aaggaaaatc
     2401 agagacggta agattgacca ctcgtctaac acataaaagt agtggaataa accacctaca
     2461 ccattgctca tctgctctgg gtctgcgaaa atatccggat agatatttac aagcagcata
     2521 ccgaatgcga tcggaacaag aagcaatggc tcaaacccct tcgcgatagc cagatacagg
     2581 aaaaagcatg cgacaatgat catcacaaaa tttccccagg taaggttaaa gaaagcggtc
     2641 tgatgcagga ggttccccat tgtctcacta aaatagctca ttgtcttcct cctaaattag
     2701 tttaatgttg caagtaatgc gcccgcttct accatatctc caacacttac gttgatactt
     2761 gctaccgtac cgtcctgcgt tgcaacgacc ggagtctcca tcttcatgat ctcaagaacc
     2821 atgatgctgt ctcccttctt aacggcatca ccgacagact tctcaagttt gaataccttt
     2881 cctgctgcgc ctgcctcgat cttaacgctg cctgcgcctg ctgcagcagc cggctttgct
     2941 gcaggtgctg ccgtcggtgc tgctgcacgt ctcggtgctg ctgccggtgc gctcactgcg
     3001 ccgttctctt ctacggttac atcatataca ttgccgttta ctgtaattgt atagcttttc
     3061 attgaaatat cctcctaata tgatttcttt tcatttatac gaattagcgt ctgcggatcg
     3121 atctcacaac gaaagaatcc gcggatgcac cggtgcttgc cgcaaccgct gcggaaataa
     3181 ctgccacaag ttcaagatca tcggtaagct gctcttcacg ctgtccgatc tgctctacca
     3241 cagcgcctgc ctctgcctcg ttcttcttgc ccttgccggc cgccttgttc tgtattacag
     3301 agataaagcg gaaacagtaa ataataaggc tgatcaggat cagaaccacg aatacggttc
     3361 ccattcccat cagggtattt aacgcagcct tctccatctt ctcacccagc gtatatacct
     3421 tgtccgcgga tgcatcagta agctccggct gctcggtctc gtaattgtat gtatagatgt
     3481 aagaaacgat gacttcacga ttctgatact ctacatactg atctactgtt aaggttttct
     3541 gtgcctttgt gatattgaat tctccaagtc cctgatatgc gccgacatca cttgtcgcct
     3601 catcccaact ctcgacaagt ttcattgccg tatcgctgcc gtaggactgg atataggttc
     3661 tgttgtcgtc cgtaagacca accagcgcgg aaacttcctg ttccatgctg ctctgcagtt
     3721 catcgtaagt cattccgaaa taatcaaccg ttgtcggatc tgttccgcat gctgcaagtc
     3781 ccagagccat aaagcaaaga caaagtataa gagataattt tttcttcata aacttcacct
     3841 ttctattttg ttccatgttt cttgtccggt gtatatgcac actttgtgta taacagttca
     3901 aacgcatcta ccagatactt tctcgtatct gccggatcaa cgatcagatc cacatagcct
     3961 cttctggctg ctgtcataac gctgccctgc agtgcatcat attcctttgc cttctctgca
     4021 agctcatccg cagatgcatc cgcgtacatg atctttactg caagatccgc atccatcatg
     4081 ccgacctttg cgtccggcca tgcatatacg aaatccgcac cgatagactt ggaattcata
     4141 gcaacataag cggtaccgta tgcctctccc gtgatcaggt tcaccttcgg acatgtagct
     4201 cccgcaaatg cggaggtcag atgagcaagt gcctttgcaa gacgcttttc agagcacttg
     4261 catgccttgt agcccttgac attcgtcaga gtcagtaccg ggatctcgaa tgcgtcgcag
     4321 aaagatacga actctgctgc cttctcacat ccctttgcgg aaagaacatt gtcaaacttc
     4381 tcgctctctt tgccctcttc gtcgtatacc gttgtgcagt ttgcaactgc gccgaccgtc
     4441 ataccgttta acttgataaa tccgatcacc atatctctgg catagcctgc ctttgtctcg
     4501 aagaatgtat ggctgtccga aatctcactg agaagatatc tcggatcacc cttcatggca
     4561 tccatgctct cgcatgcacg gttcaggtca tccgcacact cgtctgtgta gacatctccc
     4621 tcgttgttga gcggaagcat gccgaccagc tcacggatgt ctgcaaggat ctcatcctct
     4681 gttcccgtcg cgtcgatgca gccgttctca tctgcctgga atgccgcaga tgatgtgtcg
     4741 cacttctcga tgcggtttcc gtcaattgcg ttcggagaat tcacaaacat ctttcctttg
     4801 gattcttcga taaaagcaaa atcagaaagt gccggtacca ctgccagtcc accgccgcag
     4861 ctgccgaata cggctgaaat ctgcggaacc acgccggatg ctgcaacttc ttttgcatag
     4921 atcagtccga agccgtcaag cgcatctaca gactcctgta aacggactcc cgcgcaatcg
     4981 ataaagccga tcaccggtgc tcccatcttc atcgccatgt catacactgc agcgatcttc
     5041 ttcgcatgca tctcgccgat tgttccgttt aaaacagaag cgtcctggct gtaaatgaac
     5101 accagattgc cgtcaatcag gccgtgtcct gtgatgactc cgtcggaagg agtatccgtt
     5161 gctgcaagat taaaatctgt gcttcttgcg gttacgagag aaccgatctc aatgaaactg
     5221 ttctcgtcga ctaacctggt gatgcgctgc atagccaggc tatcattgct actattcatt
     5281 ctttttgccc tccatctata aattgttgtc cacatccatt tgggatgcgg cttcatagtg
     5341 aatgatgtaa atccatgcgt ctttgtataa aaattcaaca aattacgcta tgttaaatta
     5401 taattgcttt tctgcaatat ttcaagtaaa ttgttaattt tttgacgctt tttgtttgtg
     5461 ttttttattc gatccagaga agccgaagca cctgccccag acaccacgga taatcgatct
     5521 ttccgattgt ctccccggct accacacatc gctccgcgag cgtctcctcc cccagcatat
     5581 aacgaatctt cccgatgtaa tctccgctct tcaccggtgc atacagccgg tctgcaatct
     5641 catacttaag cgtaacctca ctgcttagcg gcgccaggat actctcctcc cctgcatagt
     5701 tcctgacaac cggttttaaa tacaccgttt cgccgatttg tccgccctgt ccgtcccgca
     5761 cctctatggg cgcaagctcc gcttcgtccg gctcaatctg tagcagattg agctttttga
     5821 actgctccaa tccgtaattc atgagttttc tggtgtcgga ccacttatag gtcttgttgt
     5881 tcggccatcc acaggcgagc agcgccacga tcagcgtttt gtcctcccgc tgcagcgctc
     5941 cgacatagca gtatccggcg gttccggtaa atcccgtttt tccggaaagc gccccttcca
     6001 tcatctgcaa aaatgcattg tggttcgtgc actggtacgt gcatttcccc tctgcatcag
     6061 aaaaagaata ctgcgccgtc cgtgtaatct cgagaaattc gtcgcttttt aacgattcct
     6121 taatacaata tttcataatc ttagcaaggt ctgccgccgt cgtgtgatga aacccagcat
     6181 catccttcgc atccagtccg ttcggtgtaa taaagtagct gtcctcacag ccgagttcct
     6241 ctgccttcgc attcattagc gcggcaaatc cctgcacctc ccccgccgca aattccgcga
     6301 tggccaccgc acagtcattg taggattcca gcatcaggcc atataaaaga tccctcagat
     6361 aaaactgttg tccctcgtgc atccccagat gtaccttcgg ctgggacgcc gcattttttg
     6421 acaccgtaac cacgctcgcc ggatcacagt tttccagtgc cacgatacag gttaaaatct
     6481 ttgtcgtact cgcattcgcc atcgcttcat gcccgttttt ctcatagaga attcttccgg
     6541 aatccgcatc catcaaaacg gcagattttg cgtacagttc cgactctgcc ggcacatcct
     6601 gcgcccggac acgcacaggc gaaaaaataa gcaagctcat acttaacaat agagaaagaa
     6661 agcgtcgata catgccattc tcccaaaac
//
