LOCUS       c00061_gnlPro..         5211 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NAKBJIOO_61.
ACCESSION   c00061_gnlPro..
VERSION     c00061_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 01:52:26
            Original ID  :: gnl|Prokka|NAKBJIOO_61
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5211
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..972)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg61_1"
                     /sec_met_domain="OAD_beta (E-value: 4.4e-122, bitscore:
                     396.0, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQESFLTFLGENLQLFLTYTGVYNATPGHLIMILVGLLFIFLAIK
                     YEFEPMLLIPIGFGILIGNIPFKDAGLQVGIYEQGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYTIALQMGFEPNQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPIIQPPFMRLLTTKKERLIRMKPPRAVSSTEKIIFP
                     IVGLLLTCFLVPSGLPLLGMLFFGNLLKESGVTRRLAETARGPLIDTITILLGITVGAS
                     TQATQFLTLNSIKIFGLGALSFVIATCAGVLFVKFFNLFLKEG"
     protocluster    1..5211
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:4339]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..4339
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..5211
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5211
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(977..1408)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 64.8; E-value:
                     1.6e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg61_2"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1.5e-21, bitscore:
                     64.8, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSFKYTINGNVYKVHINSVVDDIADVEVNGTPYQVKLEKPAKKQ
                     MVTLKRPAQAPTTASGDPVVTRPAASTTQGAVKTPLPGVILQVKCNVGDTVKRGQTLII
                     LEAMKMENNINADRDGKIIEIKVHKGDSVLEGADLVVIG"
     CDS             complement(1430..2317)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg61_3"
                     /sec_met_domain="OAD_gamma (E-value: 1.4e-13, bitscore:
                     40.1, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINKKIGVLLLFALLISFGAKAQRATSMRINEVLVINEDNFVDDY
                     GKRHGWIELFNTSAGTVNIAGCYLTDDKNNPMKYPIPKGDVLTQIQPRQHTLFWADGQP
                     GRGTFHVNFVLDPSKENYIALYDADGKTLIDEVTIPAGQMADISYGRVIDGKDEWAQLK
                     KVTPSTNNLTLDSNEKIDNFKQNDSLGIGMTITAMAVVFLGLFLLYIIFKQIGRLSISA
                     SKRNAQKAAGTTSVSVDAGQESGEIFAAIATALYEMSDDNHDIEHTVLTIHKVQRTYSP
                     WSSKLYGLRQTPHK"
     CDS             complement(2339..3892)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg61_4"
                     /sec_met_domain="CT_Btheta (E-value: 5e-293, bitscore:
                     960.5, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKVKELIALREQARLGGGEKRIESQHKKGKYTARERIAML
                     LDEGSFEEFDMFVQHRCTNFGIEKTKYLGDGIVTGYGTIDGRLVYIFAQDFTVFGGALS
                     EALAMKICKVMDQAMKMGAPVIGLNDSGGARIQEGVNALAGYAEIFQRNILASGVVPQI
                     SGIFGPCAGGAVYSPALTDFNIMTRGTSYMFLTGPKVVKTVTGEDVTQEQLGGASVHTT
                     KSGVAHFAVDTEEDGLQLIRKLLSFLPQNNLEETPLVECKDPIDRLDDVLNDIIPDNAN
                     QSYDMYEVIGTIVDNNEFLEVHADYAKNIIVGFARFNGQSVGIVANQPKVMAGCLDSNA
                     SRKAGRFVRFCDAFNIPLVTLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKITV
                     TLRKSYGGAYCVMSSKHLRGDMNYAWPTAEIAVMGPSGAVEVIFAKEVAAAEDPKACAA
                     EKEAEYKKAFANPYNAAQYGYIDDVIEPRNTRFRIIRALQQLQTKKLSNPAKKHDNLPL
                     "
     CDS             complement(3929..4339)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg61_5"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.3e-27, bitscore:
                     85.0, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELTHIEHLGIAVKSIEACLPYYEGVLGLKCYNIEVVADQKVKTA
                     FFKVGQTKIELLEPTSEDSTIAKFIEKKGEGIHHIAFAVPDVQAALNEAEEKGVKLIDK
                     APRGGAEGLNIAFLHPKSTCSVLTELCMPGNK"
     CDS             complement(4468..5211)
                     /locus_tag="ctg61_6"
                     /transl_table=11
                     /translation="RRVDKDKNGNNITVLNKDLKSQRSIHFILGGDYTFRAVDRNFKVT
                     AEMYYKKLDNLNPYTVDNVKIRYYGENCAKGYAMGLDVKFFGEFVPGTDSWISFSLMKA
                     QQTIRETTTVPMANSQGYNISLFFQDYFPGYKRVKLNLKGVLSGGLPQTIPGKGYEAGY
                     FRTPAYKRVDIGLSYQLAGGTDAIMDRGFFRHLKNIWLGLDVFNILDIKNVSSYYWITD
                     VYNVQYAVPNYLTGRQLNVRLIVDF"
ORIGIN
        1 accttccttc aggaacaagt tgaagaactt tacgaataaa actccggcac aagttgcgat
       61 cacgaatgat aatgcaccca gaccgaagat cttgatagag ttcaatgtca agaactgtgt
      121 agcttgggta gaagcaccta ccgtgatacc caataagatc gtgattgtat cgatcagcgg
      181 accacgagca gtctctgcca aacgacgagt cacgccactt tctttcaata agtttccgaa
      241 gaatagcata cctaacaatg gtaaaccgga cggaaccaag aagcaagtaa gcaacaaacc
      301 aacgatcggg aagataattt tttccgtgga agaaacggct ctcggcggtt tcatacggat
      361 caaacgctct ttcttagtcg taagcaatct catgaacggc ggttggataa taggaaccaa
      421 cgccatgtat gagtaagcgg ataccgcgat cgcacccatt aagtttggcg ctaacttaga
      481 agacaggaag atagccgtcg gaccgtcagc accaccgata ataccgatag cacctgcttg
      541 gttcggctca aatcccattt gaagagcaat cgtataagca ccaaagatac cgaactgagc
      601 cgctgcaccg atcaacatca acttaggatt ggagatcaat gcggagaagt cggtcatggc
      661 cccgataccg aggaagatca atggcggata ccatccagaa gtcacaccct gatagaggat
      721 gttcagtaca gacccttgct cataaatacc tacctgaaga cctgcgtcct tgaaagggat
      781 attaccgatc aagataccaa atccgatagg gatcaaaagc atcggttcaa actcatattt
      841 aatcgccagg aatataaaca acagacctac cagaatcatg atgagatgtc ccggagtagc
      901 gttgtataca cctgtatagg taaggaacag ttgtaagttc tcacctaaga atgttaaaaa
      961 gctctcttgc atagtcttat ccgattacta cgagatcagc accttctagc acagagtctc
     1021 ccttgtgtac tttaatttct ataatcttac cgtcacggtc tgcgttgatg ttgttctcca
     1081 tcttcatagc ctcaaggata atcaatgttt gacctctctt cacggtatca cctacgttgc
     1141 acttaacctg caagataaca cctggcagcg gagtcttaac agcaccttgt gtagtagagg
     1201 ctgccggacg agtaactaca gggtcgccac ttgctgttgt cggtgcttga gccggacgtt
     1261 tcaatgtaac catttgtttc ttcgccggtt tctctaattt tacttgataa ggtgtaccat
     1321 ttacttcaac atctgcgatg tcatccacta cagaattgat gtgtacttta tatacgttac
     1381 cgttaatggt atatttaaaa cttttcattc tttgttcgtt tttagataat tacttatgtg
     1441 gggtttgacg caaaccgtag agttttgaac tccaaggaga ataagtcctt tgtaccttgt
     1501 ggatcgtaag aaccgtgtgc tcgatgtcat gattgtcatc actcatctcg tacaaagcgg
     1561 tagcgatagc ggcaaagatc tcaccagact cttgtccggc gtcaaccgat acacttgttg
     1621 ttcccgcagc tttttgagcg ttacgtttgc tcgcggatat agacaatcta ccgatctgct
     1681 taaagatgat gtataacaaa aataatccca agaacactac agccatagcg gtaatggtca
     1741 taccgatacc taaagagtca ttctgtttga aattatcgat tttctcatta gaatccaatg
     1801 ttaggttatt cgtactggga gtaactttct tcagctgtgc ccattcatct ttaccatcga
     1861 tcactctacc gtaactgata tcagccattt gtccggcagg gatcgtaact tcgtcgatca
     1921 atgttttacc atctgcatca tacagagcta tataattctc cttagaggga tctaatacga
     1981 agttaacgtg gaatgtacct ctgcccggct gtccgtcagc ccaaaatagt gtgtgctggc
     2041 ggggttggat ctgagtcaat acgtctccct tcgggatcgg atatttcatc ggattgtttt
     2101 tgtcgtctgt caaataacat cccgcgatat ttaccgttcc ggccgaagtg ttgaacaact
     2161 caatccagcc atgacgtttg ccatagtcgt caacgaagtt atcctcgttg ataaccaata
     2221 cttcattaat acgcatggag gttgcgcgct gtgcctttgc cccgaatgaa atgagcaaag
     2281 cgaacagtag caatacccct attttcttat ttatcatatt catctcctgt tttacggatt
     2341 atagaggcag gttatcatgt ttcttagcag gattgcttaa cttcttagtc tgcaattgct
     2401 gcaaagcacg aataatacgg aaacgagtgt tacgcggttc gataacatcg tcgatataac
     2461 cgtattgagc tgcgttataa ggattagcga atgctttctt atattcagct tctttctctg
     2521 ctgcgcatgc tttcggatct tcagcggctg ctacttcctt ggcgaagata acctctacgg
     2581 caccgctcgg gcccataacg gcgatctcgg ctgtcggcca tgcgtaattc atatcaccac
     2641 gtaaatgttt agaactcatc acgcaataag ctccaccgta agacttacgc aatgtaacgg
     2701 taatcttagg aacggtagcc tcaccataag cgtaaagcaa tttagcgccg tgaaggatta
     2761 caccgttgta ttcctgacct gtgcccggaa ggaatcccgg aacgtcaacc aatgttacca
     2821 aaggaatatt gaaagcgtcg cagaaacgaa cgaagcgacc tgctttacga gatgcgttgc
     2881 tatccaagca tcctgccata accttcggct ggttagctac gataccgacg gattgtccgt
     2941 tgaaacgagc gaaacctacg atgatattct tagcgtagtc cgcatggacc tctaggaact
     3001 cgttgttatc gacgatcgta ccgataacct catacatatc gtaagattgg ttggcgttat
     3061 cgggaatgat atcgttcaat acatcatcca gacgatcgat cgggtctttg cactcaacta
     3121 acggcgtttc ctctaagttg ttctgaggta agaaactgag caatttacga atcaattgca
     3181 aaccatcttc ttctgtatcg acagcgaaat gggctacacc cgatttggta gtgtgtacgc
     3241 tggctccacc caactgctct tgggttacat cctcacctgt tacggttttt acgactttcg
     3301 gaccagtcaa gaacatatag ctggtaccac gtgtcatgat gttgaagtct gtcaaagccg
     3361 gagaataaac ggctccaccg gcgcaagggc cgaagatacc ggaaatctga ggaacaacac
     3421 cggaagccaa gatgttacgt tggaaaatct cagcgtatcc tgccaaggca tttactcctt
     3481 cttggatacg agctccaccg gagtcgttca aaccgataac gggagcgccc atcttcatgg
     3541 cttgatccat tactttacag atcttcatag ccaaagcctc agacaaggct ccaccgaata
     3601 cggtgaaatc ttgagcgaag atatatacca aacgtccgtc gatcgtacca taacctgtta
     3661 cgataccatc gcctaaatat ttagttttct cgataccgaa gtttgtacaa cggtgttgaa
     3721 caaacatgtc gaattcctcg aaactacctt cgtcaagcaa catggcgata cgctcacgag
     3781 cggtatattt gccttttttg tgctgtgatt cgattctttt ctctcctcca cctaagcgag
     3841 cttgctcacg aagtgcgatg agctctttta ctttttcaag ctggttactc attgtatttt
     3901 gatttgttgt tagaaatgaa cggattaatt atttgttgcc cggcatgcaa agctctgtca
     3961 atacgctgca agttgatttc gggtgaagga aagcgatgtt caagccttcc gcgccaccac
     4021 ggggagcttt atcgattaat ttaacgcctt tttcctcagc ctcgttcaaa gctgcctgta
     4081 catcaggtac cgcgaatgcg atatggtgga taccttcacc ttttttctcg ataaattttg
     4141 cgatcgtgct atcttcgctg gtcggctcta gtaattcaat cttagtctgt cctaccttga
     4201 aaaatgcggt ttttactttt tggtctgcta ctacttcgat attgtagcat tttaatccaa
     4261 gaacgccttc ataatagggt aaacaagcct cgatactctt cactgcgata cctaaatgtt
     4321 cgatatgtgt aagttccatg tcaataataa tttaaagatt tagtaaatgt ttgtttctat
     4381 tgtgtgcaaa tgtacactac tttttatgaa tgaggaaata attttttgtt tttatctccc
     4441 agtaaatgtg tttaaagata cattttatta aaaatcgaca atcagacgaa cgttcaattg
     4501 acgtccggtc agatagtttg gaactgcgta ttggacatta tatacatctg ttatccaata
     4561 gtaggaacta acgtttttga tgtctaatat gttaaataca tccagaccta gccaaatgtt
     4621 tttcaaatgg cggaagaagc ccctgtccat gatagcgtcc gtgcctcctg ccaattgata
     4681 ggagaggccg atatctacac gtttgtaggc gggcgtacgg aaatagccgg cttcgtatcc
     4741 tttgcccggg attgtctggg gaagtcctcc ggatagaact cctttcagat ttagcttgac
     4801 tcgtttatag cccgggaaat aatcttggaa gaagagtgag atgttgtagc cttggctatt
     4861 ggccatagga acggtcgttg tttcccggat ggtttgctgg gctttcatca aggagaagct
     4921 gatccatgag tcggtacccg gtacgaattc tccgaagaat ttgacatcga gtcccatggc
     4981 gtaacctttg gcgcagttct caccgtaata gcggatcttg acgttatcta cggtgtaggg
     5041 attcagattg tccagctttt tatagtacat ctcggcggtc accttgaaat tccggtctac
     5101 ggcacggaac gtataatctc ctcccaatat gaaatggatg gagcgttgtg actttagatc
     5161 tttgttcaat accgtgatat tattgccgtt cttatctttg tctactctac g
//
