LOCUS       c00343_gnlPro..         2796 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NAKBJIOO_343.
ACCESSION   c00343_gnlPro..
VERSION     c00343_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 01:52:26
            Original ID  :: gnl|Prokka|NAKBJIOO_343
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2796
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2796
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[855:2796]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      856..2796
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..2796
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2796
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             complement(2..433)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg343_1"
                     /sec_met_domain="Radical_SAM (E-value: 6.3e-14, bitscore:
                     40.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKRTTSSFRAMSTERKLESIFLFVTGRCNAKCAMCFYAQEMDK
                     KQPDLTFDEIKKISETAGQFNRLWISGGEPTLREDLPEILELFYKNNHIKDVNIPTNGL
                     KPARIVEWVKRFRQNCPDCNINVSLSLDGFGTTHDTQREI"
     CDS             709..819
                     /locus_tag="ctg343_2"
                     /transl_table=11
                     /translation="MKKPRCKYTDFSIPVKGYNAKLCFTTYNKNKKAPFF"
     CDS             complement(856..1611)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg343_3"
                     /sec_met_domain="Fer2_3 (E-value: 4.5e-27, bitscore: 82.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 8.9e-13, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 9.5e-07, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDISVTLKVWRQRGPKEKGQFETYKIEKINQSVSFLEMLDILN
                     EQLVNEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFHDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFTSVNTGGVPDANAIPIPKKDADLAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(1642..2796)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 59.1; E-value:
                     1.4e-18)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg343_4"
                     /sec_met_domain="FAD_binding_2 (E-value: 9.2e-20, bitscore:
                     59.1, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 1.3e-39,
                     bitscore: 123.3, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VQIHPTCVPVKGDFQSKLTLMSESLRNDGRIWVPKKLEDAKALQA
                     GTKKGKDIPEADRDYYLERRYPAFGNLVPRDVASRAAKERCDAGFGVNSTGLAVFLDFS
                     DAINRLGKEVVKQKYGNLFDMYEEITNDNPYETPMMIYPALHYSMGGLWVDYELMTSIP
                     GLFAIGEANFSDHGANRLGASALMQGLADGYFVLPYTIQNYLSDQIQVPRFSTDLPEFV
                     EAEKAIKDRIQKLMNVKGKETVDTIHRKLGHIMWEHIGMARDAKGLQEAIQMLKDLKKE
                     FWSNVFIPGNADNLNTELEKALRVADFIEIGTLMAHDALDRAESCGGHFRTEHQTEEGE
                     ALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNYEFVVPQTRNYKK"
ORIGIN
        1 cgatctctct ctgtgtatcg tgggtcgtac cgaaaccatc caaggaaagg cttacgttaa
       61 tattacaatc cgggcagttc tggcggaacc gttttaccca ttcgacgatc cgggctggtt
      121 ttaagccgtt cgtagggatg ttcacatctt tgatatggtt gtttttgtag aataactcca
      181 atatttccgg tagatcctca cgcagggtag gctcacctcc ggatatccat aatcggttga
      241 attgcccggc ggtttctgat atcttcttga tttcatcgaa agtgaggtcg ggttgcttct
      301 tatccatttc ttgggcatag aaacacatcg cgcatttggc gttgcatcgg cctgttacga
      361 aaaggaagat cgactcgagt tttctttccg tgctcattgc cctgaaagaa ctggtggttc
      421 gttttttgtc cattttattt ttgttttacc atttgtctta tctggcgcat gattttttcc
      481 ttgtgtttga tcaacacccg gagaaaataa cataagaagc caaataaaat acttattagc
      541 atatactata tgatctttaa aggcaaagat cgacgataaa atgacgctaa acaatctcta
      601 tttgcagtat tttatggtcc atgtttaaaa ggtaggtcta taaacaatta aagcctgtct
      661 cacgacaagc cctaaccaaa ctttgttaac cttaaatcta atactattat gaaaaaacca
      721 cgatgcaaat atacggactt ttcaattcct gtcaagggct ataatgctaa attatgtttt
      781 acgacatata acaaaaacaa aaaggcgcct ttcttttaga gaaagacgcc ttttccatga
      841 tatactaatc aaatattagt cttgtaattt agcgcaaatg aactcgcggt tcaaacgagc
      901 gatgttgcta acggagatat tcttcggaca ttccatctcg caagcacgtg tgttcgtaca
      961 gttaccgaag ccaagctcat ccattttagc gaccatagct ttggcacggc gagcagcctc
     1021 gactttacct tgaggcaaca aagccagctg gctaacctta gcggatacga ataacatagc
     1081 ggaaccattc ttacaagcgg cagcacaagc gccacaaccg atacaagcgg cagcgtccat
     1141 ggctaagtct gcgtccttct ttgggatagg tatcgcatta gcgtcgggta caccacctgt
     1201 gttgacagat gtgaaaccac ccgcttggat gattttgtcg taagcgtagc ggtctaccat
     1261 caagtcgcgg ataacaggga aacctgcgga acgccacggt tcgatcgtga tcgtctctcc
     1321 atcgtggaaa cgacgcatat ataactggca agtcgtagct ccagttgccg gtccgtgcgg
     1381 gtgtccgttt acatacaatg aacacatgcc acagataccc tcgcggcagt cgtgatcgaa
     1441 tacgatcggt tccttaccct cgttgaccaa ctgctcgttc aagatatcca acatttccaa
     1501 gaaagagaca ctctgattga tcttctcaat tttgtatgtc tcgaactgtc ctttctcctt
     1561 cggacctctc tgacgccaaa ccttaagtgt tacacttata tctttatcca tgattctaat
     1621 tttttctgag attgatgatt attacttctt gtagttacgt gtttgaggaa caacgaactc
     1681 atagttcaaa tcctctttca acatgacagg atctttgtcc tcgccttggt actcccagca
     1741 agatacgtac atgaacttat cgtcatgacg caaggcctca ccttcttctg tctggtgctc
     1801 cgtacggaag tgaccaccac aagactcagc gcggtccaaa gcatcgtgtg ccatcaatgt
     1861 accgatctcg ataaagtcgg ctacacgtaa tgctttctct aactcggtat tcaagttgtc
     1921 tgcgttgcct gggatgaata cgttgctcca gaactctttc ttcaagtctt tcagcatctg
     1981 gatagcttct tgcaaacctt ttgcgtcgcg tgccataccg atatgttccc acatgatatg
     2041 acctagtttg cggtggatag tgtctacggt ttctttaccc ttgacgttca tcaatttttg
     2101 gatacgatcc ttgatggcct tttctgcctc gacgaactcc ggcagatccg tgctgaagcg
     2161 aggaacttgg atttggtcgg acaaatagtt ctggatcgtg tatggtaata cgaaataacc
     2221 atcggccaat ccctgcatca aagcggaagc acctaaacgg ttcgctccgt gatcggagaa
     2281 gtttgcctca ccgatagcga acagacccgg aatggaggtc atcaactcat agtcaaccca
     2341 caatccaccc attgagtagt gcaatgccgg gtagatcatc atcggagtct cgtatggatt
     2401 atcgttcgtg atctcttcgt acatatcgaa caagttaccg tatttctgct taacgacctc
     2461 tttacccaag cgattgatcg cgtcggagaa atccaagaat acggccaagc ccgtgctgtt
     2521 cacgccgaaa cctgcgtcgc aacgctcttt agcggcacga gaagccacgt cgcgaggaac
     2581 caagtttccg aatgccggat aacgacgctc caaataataa tcacggtctg cctcgggaat
     2641 atctttacct ttcttcgttc cggcctgtag agctttagcg tcttccagtt tcttcggaac
     2701 ccagatacgg ccgtcgttac gcaaggattc ggacatcaaa gtcaatttag attggaagtc
     2761 acctttcacc ggtacgcaag tcgggtggat ctgaac
//
