LOCUS       c00189_gnlPro..         3754 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AOJOFOOJ_189.
ACCESSION   c00189_gnlPro..
VERSION     c00189_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 07:08:18
            Original ID  :: gnl|Prokka|AOJOFOOJ_189
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3754
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3754
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1257:3753]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1258..3753
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3754
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3754
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..1009
                     /locus_tag="ctg189_1"
                     /transl_table=11
                     /translation="FAPQDKKTAALLENMELKPYRILVAVPRALDEALATVPLIRALKT
                     VRCDMQVNIVCPSAQLGIWKTVPEVTHVLPHDSLKQLREALAADEFYNDGPLDLGVMLD
                     GDMETLKALEPYGPMMFSGLDTHPGARKYKFRVKAPVLRAAPPAHRVQLYLQLGGLHGL
                     DAATPSLFPVKKAAPAEDAPILIAPFSRLGSASEWSWEQWRELVSLLPGRTVLAALEED
                     RERAASLAEHLNVELAVGAPEALFPVMDGAAAAVAVDGDLPSLCSFRGLPVVTLFSTRL
                     PDVYRPMGTFNRSLYSHQCCSPCFRKECDRDVPCNRHIAVRDVLEALREISGKE"
     CDS             complement(1258..1644)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg189_2"
                     /sec_met_domain="Radical_SAM (E-value: 9.2e-10, bitscore:
                     27.3, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLADTDLVLLDIKAWNPKRYRALTGGELHPTLEFAERLASLRKPV
                     WLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKWDELKLDYTLKDVP
                     EASAELAEQVRGIFRQGGFPDCVH"
     CDS             complement(2025..2789)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg189_3"
                     /sec_met_domain="Gly_radical (E-value: 6.3e-47, bitscore:
                     146.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 5.7e-48, bitscore:
                     151.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHDKYCYERIEMALHDPEILRTMATGIAGLSVAADSLSAIKYATV
                     KAIRNEEGLIVDFKTEGEFPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYRNSLPTQSI
                     LTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDRNGAVASMLSVAKLSYDDSLDG
                     ISYTFSIVPQALGKEERERRVKLVSLLDAYFAATGHHINVNVLERKTLLDAMDHPEKYP
                     QLTIRVSGYAVNFIKLTREQQQEVINRTFHTR"
     CDS             complement(2767..3753)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg189_4"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.5e-148, bitscore:
                     483.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VQTCALPILYGTDKLIAERRKDLKNREHSPLTDELIRLREEMSEQ
                     IRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGRVSTFFDI
                     YFTRDLEQGLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTESIGGMGED
                     GRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSSVQYENDD
                     LMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGKQVAPPSP
                     RYTEEILNYDEVMALYDKMQDWLAKTYIDALXXXAEHHPLHARQILL"
ORIGIN
        1 atttgccccg caggataaaa aaacggctgc gctcctggaa aatatggagc tgaagccgta
       61 ccgcattctg gtagccgttc ccagggcgct ggatgaagcc ctggcgactg ttcccctgat
      121 tcgagctttg aaaaccgtcc ggtgcgacat gcaggtgaac atcgtctgcc cgtctgccca
      181 gctggggata tggaagacgg tgccggaagt cacccatgtg ctgccgcacg actccctgaa
      241 gcagcttcgg gaagctttgg cagcagatga attttacaat gacggaccgc tggatctggg
      301 cgtcatgctg gatggagata tggaaaccct gaaagcgctg gaaccgtatg gccccatgat
      361 gttttccggg ctggacacgc atcccggtgc ccggaagtac aagttccggg taaaggcccc
      421 cgtcctgcgc gccgcccccc cggcgcaccg ggtgcagctt tatctgcaat tgggggggct
      481 gcatgggctg gacgccgcca ccccgtcttt gttccctgtg aaaaaagcgg ccccggcgga
      541 agatgccccc attttaatcg ctccgttttc ccgcctgggg agcgccagtg aatggagctg
      601 ggagcagtgg agggagctgg tttcccttct gccgggacgg acggtgctgg cggctctgga
      661 agaagaccgg gagcgcgccg cgtccctggc ggaacatttg aatgtggagc tggcggtggg
      721 tgcgccggaa gctctctttc ccgtcatgga cggcgctgcg gcggcagtag ccgtggatgg
      781 cgacttgccg tccctctgtt ctttccgggg gcttccggtg gtgactctgt tttccacccg
      841 gcttccggat gtttaccgcc ctatgggcac attcaaccgt tccctttaca gccaccagtg
      901 ctgttctccc tgcttccgga aggaatgtga ccgggatgtt ccctgcaacc gccacattgc
      961 cgtgcgggac gtactggaag ccttgcggga aatttccggg aaagaataat ctgggaaggg
     1021 acggaatgcc gtcccccggc gttggcggaa aaaacggcgg gaatgctgcc gttgccgtaa
     1081 agaatgatga aataacgaaa ggaattgcgg aaatgccgta ttccggagca attccagaaa
     1141 tgcgtcttcc tttttgcatg gattctgcag tggaggggag agggcccctc atccggaaga
     1201 gggggatgcc tgcgtccggt ttgctgcggt tggtggcgcc aaagcgcgaa aatccggtta
     1261 atgcacgcaa tccggaaagc cgccctgccg gaaaatgccg cggacctgtt ccgccagttc
     1321 tgcggacgct tccgggacgt ccttcaaggt atagtccagc ttcaattcat cccatttgaa
     1381 tctccccagc tggtggaaag ggagcacgtc cacgcgttcc acgttgccca gaagcgccgc
     1441 atggcgggag agttcctcaa tttcttccag attgtccgta acgccaggta ccagcacata
     1501 cctcagccac acgggcttgc ggagggaggc caggcgttcc gcaaactcca gtgtagggtg
     1561 cagttccccg cctgtcaggg cgcgataccg tttcggattc catgccttga tatccagcag
     1621 gaccagatcc gtatccgcca gcatttcttc gcccgcattc acgcccagat ggccggannn
     1681 nnnnnnnttt cagcacggca ccggcaaaat ccggctggaa gagggggtcc ccgccggaga
     1741 gggtgacgcc gccgcccgcc gcctgcaaaa aatcccggta acgggctatt tccttgagga
     1801 catcatctgc gctcctgagc cggcccctcc gcacgtagga ggtatccgga ttatggcagt
     1861 agcggcagcg caggctgcaa ccggacagga aaaggacgaa gcggatgccg gggccgtcca
     1921 cggtgccgca ggattccacg gaatgaacca aaccggtgac ggagctttct ccgtccggat
     1981 catggttttg tggaaaaacc tgcggcatcc gcttgcagtc cgggttaacg ggtgtggaaa
     2041 gtgcggttga tgacctcctg ctgctgttcc cgggtcagct tgatgaaatt cacggcatag
     2101 ccggaaacgc ggatggtaag ctgcgggtat ttttccgggt gatccatggc gtcgagaagc
     2161 gttttccgtt ccagtacgtt cacattaatg tggtgacctg tagcggcaaa gtaggcgtcc
     2221 agcagggaga cgagcttgac gcggcgttca cgttcttcct tgcccagggc ctgaggaacg
     2281 atggagaagg tgtaggagat gccgtccagg gagtcgtcat aggacagctt ggctacggag
     2341 agcatggagg ccacggctcc gttcctgtcc cttccgtgca tggggttggc tccgggggcg
     2401 aacggttccc cggctcggcg gccgtccggc gtgttgcccg tctttttgcc gtacaccacg
     2461 ttggaggtga tcgtcaggat ggactgggtg ggcagggaat tgcggtaagt gtggagcctg
     2521 cgcagttttt ccatgaaatt gctgaccagg ctgcatgcta tgtcgtccac gcgcgggtca
     2581 ttgttcccgt aacaggggaa ttccccttcc gtcttgaagt ccacaatcag cccttcttca
     2641 ttgcggatgg cttttacggt ggcatacttg atggcggaca gggagtccgc cgccacggaa
     2701 agcccggcga ttcccgtagc catcgtgcgc agaatttccg gatcatgcag cgccatttca
     2761 atgcgttcat agcagtattt gtcgtgcatg tagtggatga tgttcagcnn nnnnnnnnag
     2821 cgcgtcaatg taagttttgg cgagccagtc ctgcatcttg tcgtacaggg ccatcacctc
     2881 atcatagttg agaatttcct ccgtgtagcg gggggagggc ggggccacct gcttgccctt
     2941 gagttcatcc acgccgccgt tcagggcgta gagaaggcac ttggcgaggt tggcgcgcgc
     3001 tccgaagaac tgcatctgct tgccgatgcg catggcggac acgcagcatg cgatgccgta
     3061 gtcgtcgccc caatgcgggc gcatcaggtc gtcgttctcg tattggacgg aggacgtttc
     3121 aatggaaacc ttggcgcaga agcttttgaa ggcttccggc aggttccggg accacagaac
     3181 ggtcaggttc ggttccggag ccggccccag gttgtacagg gtctgcagca tgcggaagga
     3241 actgcgggtg acgagggtgc gcccgtcctc tcccatgccg ccgatggatt ccgtcaccca
     3301 ggtggggtct ccggagaaca ggttattgta atcaggcgtg cggatgaaac ggacgatgcg
     3361 cagcttcatc acgaactggt cgatgatttc ctgaacttcc tcttccgtga tgagcccctg
     3421 ttccaggtcg cgggtgaaat aaatgtcgaa gaaagtggaa acgcggccca gggacatggc
     3481 cgctccattc tgttccttca cggcggctag gtagcccagg tacgtccatt gcacggcttc
     3541 gcgggcgttt gcggcggggc gagtgaggtc gcagccgtag gaggcgccca gctgggcgag
     3601 ttcttccagg gcgcgtattt gttcgctcat ttcttcgcgc agacggatca gctcatccgt
     3661 aaggggggaa tgttcacggt ttttgaggtc cttgcggcgt tcggcaatca gcttatccgt
     3721 gccgtacaag atcggaagag cacacgtctg aact
//
