LOCUS       c00061_gnlPro..         5670 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ODPKDEEL_61.
ACCESSION   c00061_gnlPro..
VERSION     c00061_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 03:28:58
            Original ID  :: gnl|Prokka|ODPKDEEL_61
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5670
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..372)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 68.8;
                     E-value: 1.9e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg61_1"
                     /sec_met_domain="Radical_SAM (E-value: 1.4e-25, bitscore:
                     78.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLV"
     protocluster    1..5670
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2613]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2613
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5670
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5670
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(373..2613)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg61_2"
                     /sec_met_domain="Gly_radical (E-value: 1.6e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.3e-282, bitscore:
                     925.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRRVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             complement(2784..5087)
                     /locus_tag="ctg61_3"
                     /transl_table=11
                     /translation="MEQCEENSQENDDVIIEKEISKLIEEQRLFTESIYACLPVGIEIY
                     DAHGVLRSINDHALRMYGVDDRTSVVNIVNLYKSPFVDSELLARIQSGEDISLEFEYDF
                     DRVNKDAYYSSYNKDTMIYGVKVIPIWSRKKHLIGHILLANDMTSVKEVEFRTEESKKN
                     LEMAMKAANMASWVYDVNKKVFNPLYGLTVAKKNMPMEAVLDILHPQDRAPLIRLFSQL
                     IGNEIEQGQMTLRFYNEQEEMYRYYESRMRLSTEHRGKLQIIGTQMDITERLQMAKKAQ
                     DLIAKRDLAMKVSNIVHWDFDVNSQKFESYNDPINDYVSDRLLTVSEYMDVIYPEDRSV
                     FYDAMQSLIAGKNVTINFTCGIQTKYDETWQYCDFMGVPFDQDENGDIIRYTGFRQNIP
                     KIQQLNRELKERNYKIELSFKTVGMSYWDFDVKSLKFSAFNDPINDFYSEKTITPEDYL
                     NATHPDDVVFVRKYMEHMFRGIDKDFNVKYRSKTKWDDEWQTLLVTGIQVERDKEGHVT
                     RYTGITINNTKWEKMIQQLKELKEKAELSDRLKSAFLANMSHEIRTPLNAIVGFSELMV
                     TCDDPEEKKEYINIIQSNNELLLRLINDILDLSKIESGIIERRKENFNLAKVCNELFVT
                     IQAKMTNPNVELRLDGPNSECWVLLDRNRLKQVWMNFLTNAVKCTKSGYIKMGYGIERK
                     GLRIYVEDTGIGIPDDLHDKVFTRFEKLNEFSQGTGLGLTISRAIVEAAGGEVGFTSKL
                     GVGSTFWAWLPLRP"
     CDS             5244..5669
                     /locus_tag="ctg61_4"
                     /transl_table=11
                     /translation="MRFTRLILALTSFLILITQTDLFASTVLHKTSMVDLSNNAIICMH
                     QDPDGYLWIGTYDGLNLYNGKDTYVYRFELNNKNSLCSNIIHKISDAEPGFLWISTSLG
                     INKFSLKERKVTESFPGYMECDLLASDSSGITLAICKE"
ORIGIN
        1 cactaagtcc gcataatcca agacttccca agcttttgag gtacatacga aaccagatgt
       61 gtccaaagct gtgtgaagac cttgctcttg gcacaaacgg aaaaactctt tcagaaactc
      121 aggttgcaat aaaggttccc caccggtaac agtcaccccc ccacgggcga taaaactctt
      181 ataccgtaag acttccgtca atagctcgcc gggagtcatc tgatatttca ccttcccttt
      241 cgggttccaa gtatccgggt tatggcaata caggcaacgc aacgggcagc cctgcataaa
      301 aacgacaaag cgaattccgg gaccatctac ggtcccgaaa ctctctaatg aatgtatctt
      361 accttttatc acttacagtg aatggttaat ggttcttgaa ataacatcca actgttgttc
      421 acgagtcagt ttcacgaagt ttaccgcata tccggataca cggatcgtaa gttgcggata
      481 tttctcagga tgttccatag cgtcgatcaa caaatcctta tcaaatacgt taacattcag
      541 atgctgacct ccatccggag taaaatagcc atccaataag ctaaccagat tattgatctg
      601 cacttcacgc tcctttccca acgtagccgg agaaacagcg aaagtataag agataccgtc
      661 gtgcgcatgc tggaatggaa gcttcgctac cgaagctagc gcagcgatag cacctttcgt
      721 atcacgaccg ttcatagggt tcgcgcccgg agcgaaagga gttccaccga tacggccatc
      781 cggtgtcgta ccagtcttct taccatatac cacattagag gtgatcgtaa ggatggactg
      841 cgtaggtttc gcatggcgat aggtctcgtg ctgacgcaaa tattccatga acttctcggt
      901 gatctctaca gcgatactat ccgtacgatc gtcgttatta ccgaaaggaa catattcgcc
      961 ctcacgttcg aaatcaaccg ccaagccacg ctcgtcacgg atcacacgta ctttcgtatc
     1021 acggatagca gccaacgaat cggcaacgat agaaagaccg gcgatacctg tcgcacggat
     1081 acgctctaca tctccatcgt gaagcgacat ctcgaatgcc tcatatgcgt atttatcatg
     1141 catataatgg attatcttca aggcgttcac atacactttc gccaaccagc gcatcatttg
     1201 ctcatactta gccatcacct catcgtattc cagatactcg ccgcgaaccg gctcgaacat
     1261 cggggctacc tgtacgccgc tgcgctcatc ccgtcctccg ttgatcgcat ataataaaca
     1321 tttcgccaaa ttcgcacggg ctccgaagaa ctgcatctgc ttaccgatct tcataggaga
     1381 aacgcaacaa gcgataccat aatcatctcc ataatccgga cgcatcaaat cgtcgttctc
     1441 atattggata gccgatgtat cgatagagac cttggcacag aaacgtttcc agttctcggg
     1501 agcgttctta aaccacagaa ccgtcaaatt gggttctggt gccggaccta gattatataa
     1561 tgtatggaga taacgatagg aagttcttgt taccaaagaa cggccatcca ctccttggcc
     1621 gcccaaggac tctgttaccc aaaccggatc accggagaac aaatcattgt attccggcgt
     1681 acgtaagaaa cgaacgatac gcaacttgat gatcagctgg tctaccaatt cttgcgcctc
     1741 ctcttcggtc aacttaccct cacggatgtc tttctcgata taaatatcca aaaacgtaga
     1801 agtacgtccg atagacatcg ccgcgccatc ttggtctttt accgccgcca gataagctaa
     1861 gtacacaaac tgaactgcct cacgagcgtt ttgtgccgga cgagtcacat caaagccata
     1921 gctagcgcac attttctcga acgctttcaa agccttgatc tgttcggaaa tctcctcacg
     1981 gctctggatg atctcttccg tgatctcttg gatatccaaa cgattcaaga aacgcttttt
     2041 ctcctcgatc aagattgccg taccatataa agcgacacga cgatagtcac caataatacg
     2101 tcctctacca tacgcgtcgg gcagaccggt gataatagcc gaacgacgtg cggcaatcat
     2161 ctcttccgta tatgcggaga aaacgccttc gttatgagtt ttacggtact tcgtgaagat
     2221 ctctttcgtc atcgggtcca aatgataacc ataagcttcc aagctattct caaccatacg
     2281 gataccgccc ttcgggaaga taccacgttt cagcggagca tcggtttgta acccaacgat
     2341 cacctcattc tccttatcaa tatagcccgg gccatataca tcgatccctt gaggtaattt
     2401 tgtctcggcg tcataaactc ccttttccct ctcgacctta aacatctcgg tcagcttgtt
     2461 ccagacccta cgggtcttct cggaagaagc gactaaaaag gaatcatccc cttcataagg
     2521 cgtatagtta ctctggataa aatctcttac attgatctct cttttccagg tctcaccttt
     2581 aaagtgatcc cagcactcat tctcaaattt cataaacaat cttatctaat accacatttt
     2641 taccgccgca aaggtaggag aattatcgtc atatttctgt cacatatgct acaccagttc
     2701 taaaatccct ataaattgcg ataaaaaagc ttctcatcct tcactatttt aaaaggacat
     2761 taccaataaa tggggagtca gacctaaggg cgaagaggca accatgccca aaaagtagat
     2821 ccgacaccaa gttttgaagt aaagcccact tctccccctg ccgcttctac gatagctctg
     2881 gagatcgtca agccaagtcc ggtaccctgc gagaactcat tcaatttctc aaaacgggta
     2941 aatactttgt catgcaaatc atccggtata ccgattcccg tatcctcaac atagatacga
     3001 agacctttcc tttcaatgcc atatcccatt ttaatatatc cggatttggt gcattttacc
     3061 gcattagtga ggaaattcat ccatacttgc ttaagccggt ttctatccag aagtacccag
     3121 cactccgagt tgggaccatc taaacgtaac tcgacattcg gatttgtcat cttcgcctga
     3181 atcgtaacaa ataactcatt acaaacctta gccagattga aattctcctt cctacgttca
     3241 attatgcctg actctatctt ggaaagatca aggatatcgt tgatcaatcg caataacaac
     3301 tcattattgg attggatgat atttatatat tctttttttt cttccggatc atcacaagtt
     3361 accattaact ctgaaaaacc aacaatagcg tttaacgggg tacgtatctc gtgactcata
     3421 ttagcgagaa acgcagactt gagacgatcc gaaagttccg ctttctcctt taattccttc
     3481 aattgctgaa tcatcttctc ccacttcgta ttattgatcg taataccggt atagcgagtc
     3541 acatggcctt ctttatctct ttcaacctgg attccagtaa ccaataaggt ctgccactcg
     3601 tcatcccatt tggttttgga acgatattta acgttgaaat ctttatctat gccccggaac
     3661 atatgctcca tatatttccg cacaaaaacc acatcatccg gatgtgtagc gttcaaataa
     3721 tcctcgggag tgattgtctt ttccgaataa aaatcattaa taggatcatt aaaagcactg
     3781 aatttcaggc tttttacatc aaaatcccaa tacgacatac caaccgtctt gaaagaaagc
     3841 tctatcttgt aatttctttc tttaagttcc cgattcaatt gctggatctt aggaatgttt
     3901 tgcctgaatc ccgtatagcg tattatatct ccattttcat cttgatcgaa agggacaccc
     3961 ataaaatcac aatactgcca agtctcatca tacttggttt gtatgccaca ggtaaaattg
     4021 atggtgacat tctttccagc tatcagggat tgcattgcgt cgtaaaaaac agaccggtcc
     4081 tcggggtata taacatccat atactccgaa acggtcaata atcgatctga aacgtaatcg
     4141 ttgatcggat cattataaga ttcgaatttc tgggaattta catcaaaatc ccaatgaaca
     4201 atattactga ctttcatggc cagatcacgt tttgcgatca gatcctgcgc tttcttggcc
     4261 atttgcaacc tttcggttat atccatttgt gtcccgatga tctgcaattt tccacgatgt
     4321 tctgtcgata ggcgcatccg gctctcataa taacgataca tctcttcttg ctcgttatag
     4381 aaacgcaaag tcatttgtcc ttgttctatc tcatttccta tcaattgaga gaagagccgg
     4441 attaacggag ctcgatcttg gggatgcaaa atatccagta ccgcctccat tggcatattt
     4501 tttttggcta cggttagtcc atataatgga ttaaaaacct ttttattcac atcatatacc
     4561 caagaagcca tattagccgc tttcatggcc atctccagat ttttcttgct ttcttccgta
     4621 cggaattcca cctctttcac ggaagtcata tcatttgcga gtaatatatg tccgataaga
     4681 tgtttttttc tactccatat cgggataact ttaaccccat agatcatcgt gtctttatta
     4741 taggaagaat aatatgcatc cttattaacc cgatcaaaat catactcaaa ctccaaggaa
     4801 atatcttcac ccgattgtat ccgagctaaa agttctgaat caacaaatgg acttttgtaa
     4861 agattgacta tattcaccac cgatgtccgg tcatccacac catacatcct caacgcatgg
     4921 tcgtttatac tacgcagcac accatgtgcg tcataaattt caatacccac cggcagacaa
     4981 gcatatatac tctccgtaaa taaacgctgt tcttctatta atttagaaat ttctttctct
     5041 attatcacat cgtcattttc ttgagaattc tcttcacatt gttccatctg tcacttactt
     5101 tatttcgtat tatcatacgt cagacattct aatttatacg aaagccctgc tttttgcaaa
     5161 agtaaacaaa aaagtgaatt cttttctgtt cgttcataaa gatattatct ttgcgctgaa
     5221 aacaaacact aaatcttaaa tacatgagat tcacaaggct tattctagcg ttaaccagtt
     5281 tccttatatt gatcacgcaa acagacctat ttgcctcaac cgtattgcat aaaacttcta
     5341 tggttgatct ctcaaataac gctattattt gcatgcatca agatccggat ggctatttgt
     5401 ggatcgggac ttacgacgga cttaacttat acaacggaaa agatacgtat gtatatcgtt
     5461 tcgaattaaa taacaaaaac tctctttgta gtaatatcat tcacaaaata tccgacgcag
     5521 aacccggctt tttatggata tcaacctctt taggtatcaa taaattctca ctaaaagaac
     5581 ggaaagtcac cgagtctttc cccggataca tggaatgcga tctattggcc tccgattcat
     5641 ccggcatcac gctcgctatt tgtaaggaaa
//
