LOCUS       c00278_gnlPro..         4799 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AGCANJKK_278.
ACCESSION   c00278_gnlPro..
VERSION     c00278_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 01:58:37
            Original ID  :: gnl|Prokka|AGCANJKK_278
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4799
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..213)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg278_1"
                     /sec_met_domain="OAD_beta (E-value: 7.6e-16, bitscore:
                     46.6, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDFINFLGNNLADFWTYTGFANATGGHIAMIIIGLGFIYLAVAK
                     EFEPMLLIPIGFGILIGNIPFNMDAG"
     protocluster    1..4799
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:3614]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..3614
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..4799
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4799
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(215..649)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 64.8; E-value:
                     1.7e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg278_2"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1.6e-21, bitscore:
                     64.8, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEYKYKINGNSYKVTIGDIEDNIAHVEVNGTHYKVEMEKQPKAT
                     PKPAVVRPMPNSPAAPTTPVVKPAAPSTGKSGVKSQLPGVILDIKVNVGDTVKRGQTII
                     ILEAMKMENNINADKDGKVTAINVNKGDSVLEGNDLVIIE"
     CDS             complement(674..1594)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg278_3"
                     /sec_met_domain="OAD_gamma (E-value: 1.7e-15, bitscore:
                     46.2, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTKIGIFLSLLLLIGLTSCGEQKSNNKLLLNEVLIDNQNNFQD
                     DYGLHSAWIEIFNKSYGSADLAACLLKVSSQPGDTVTYFIPKGDILTLVKPRQHALFWA
                     DGEPNRGTFHTSFKLNPETANWVGLFDSGRKLLDQVVIPAGTLGPNQSYARISDGAADW
                     EVKGGSKDKYVTPSTNNKTLDSNAKMEKFEEHDSVGIGMSISAMSVVFCGLILLFIAFK
                     VVGKVAVNLSKRNAMKAKGIDKVEAKELSQAPGEVYAAISMALHEMQDEVHDVEETVLT
                     ITRVKRSYSPWSSKIYTLRENPNRK"
     CDS             complement(1626..3179)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg278_4"
                     /sec_met_domain="CT_Btheta (E-value: 2e-291, bitscore:
                     955.2, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKIKELIERRAVARIGGGEKAIAKQHEKGKYTARERIAML
                     LDEGSFEEMDMFVEHRCTNFGMEKKHYPGDGVVTGCGTIEGRLVYLFAQDFTVTAGSLS
                     ETMSLKICKIMDQAMKMGAPCIGINDSGGARIQEGINALAGYAEIFQRNILASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMMEGTSYMFLTGPKVVKTVTGEDVSQENLGGASVHST
                     KSGVTHFTAKTEEEGLALIRTLLSYIPQNNLEEAPYVDCTDPIDRLEDSLNDIIPDSPN
                     KPYDMYEVISAIVDNGEFLEIQKDYAKNIIIGFARFNGQSVGIVANQPKFLAGVLDSNA
                     SRKGARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTI
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVEVLYAREAKDQENPAQFLA
                     EKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRVIRALQQLQTKKLSNPAKKHGNIPL
                     "
     CDS             complement(3210..3614)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg278_5"
                     /sec_met_domain="Glyoxalase_4 (E-value: 6.4e-25, bitscore:
                     76.4, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKISHIEHLGIAVKSIEEALPYYENVLGLKCYNIETVEDQKVRTA
                     FLKVGETKIELLEPTCPESTIAKFIENKGAGVHHVAFAVEDGVANALAEAESKEIRLID
                     KAPRKGAEGLNIAFLHPKSTLGVLTELCEH"
     CDS             complement(3788..4798)
                     /locus_tag="ctg278_6"
                     /transl_table=11
                     /translation="ICIWMPLKLTTYYRGKDIPDLPGTNTFHSKELFQIYESTPGYTPL
                     LIVATEEGRPVARLLAAIRKTKKWLPSSLVKQCVVYGAGEFLDTSLPASKEKEEEIFGE
                     MLEHLTQEASRTCILIEFRNLDNSMFGYRFFRKNDFFPVNWLRVRNSLHSTQKAEDRFS
                     PSRIRQIRKGLKNGAKVVEAHTVEEIKEFSRMLHKVYSSRIRRYFPANDFFRHMNSMLI
                     RGKQAKIFIVKYKEKIIGGSVCIYSGENAFIWFSGGMRKTYALQSPGILAVWKALEDAH
                     ERGFRHMEFMDVGLPFRKHGYRDFVLRFGGKQSSTRRWFRISWPWLNNLLVKFYV"
ORIGIN
        1 tccggcgtcc atattgaaag gtatattacc gatcagaata ccgaatccga taggaattaa
       61 caacatcggc tcgaactctt tggctacagc taagtaaata aagcctaaac cgataatgat
      121 catagcaata tgtccacctg tcgcgttggc aaatcccgtg taggtccaga aatcggcaag
      181 gttatttcct aaaaagttaa taaaatctcc catattattc aataattacg aggtcattac
      241 cttcaagaac agaatctccc ttgttgacat tgatggcagt cactttaccg tctttgtctg
      301 cattgatatt gttttccatc ttcatggctt ccaatataat gatagtctgt cctcttttca
      361 ctgtatcacc tacatttact ttgatgtcaa ggataacgcc tggcagttgg gacttaacgc
      421 ctgatttgcc ggtagaagga gcagccggtt ttactaccgg tgtggtagga gcagccggtg
      481 agttaggcat agggcgtact actgccggtt tgggagtagc tttcggttgt ttctccattt
      541 ctactttgta atgcgtaccg ttcacttcta catgagcgat attatcttca atatctccga
      601 tggttacttt gtatgagtta ccgttgattt tatatttata ttctttcatc gttttatctt
      661 tttagggaat gatttacttt ctgttaggat tttcacgcaa ggtgtaaatc ttcgaactcc
      721 acggtgaata actgcgcttc acacgagtga tggtcagcac cgtttcttct acgtcgtgta
      781 cctcatcctg catttcgtgt aatgccattg aaatagcagc ataaacttca cccggagcct
      841 gtgaaagctc tttcgcttcg actttatcga tgcctttagc cttcatcgca ttgcgtttgc
      901 tcaggttgac agcaacttta cctaccacct tgaaggcaat gaagagaaga atcaatccgc
      961 agaatactac gctcatggct gaaatagaca taccgatacc gactgagtca tgttcttcga
     1021 acttttccat cttggcatta ctgtcaaggg ttttgttgtt cgtgctcggg gttacgtatt
     1081 tgtctttgct tccgcctttt acttcccagt ctgctgctcc gtcgcttatg cgtgcataag
     1141 actggttggg acctaaagtt cctgctggta tgacaacttg atcaagtagc tttctgcctg
     1201 aatcaaaaag accgacccaa ttggctgttt cgggattcag tttgaagctg gtatggaagg
     1261 ttccacgatt aggttcgcca tctgcccaaa ataacgcatg ttggcgtggc tttactaatg
     1321 taagtatgtc accttttggg ataaaatagg ttactgtatc gcccggctgg ctgcttactt
     1381 tcaataaaca agctgctaag tctgcgctac cgtatgattt gttgaatatt tcaatccatg
     1441 cactgtgcaa gccgtagtca tcctgaaagt tgttttgatt gtctatcagg acttcattca
     1501 gcaatagctt attgttcgac ttttgctctc cgcaggaagt caatccaatc agcaacagca
     1561 gagaaaggaa tattccgatt ttggttttgt tcataatcgt tcttgtttta atgtgaaaag
     1621 ttggattaca acggaatatt accatgtttc ttcgccggat tagacagttt cttagtctgc
     1681 aactgctgca aagcacggat cacgcggaaa cgtgtgttac ggggttcgat gacatcatcg
     1741 atgtaaccat acttagctgc attataaggg ttggcaaaca gttttgtata ttctgcttct
     1801 ttttcggcaa ggaactgagc cggattttcc tggtctttgg cttcacgcgc gtacaatact
     1861 tctactgcac ctgcaccacc cattacagcg atttcagctg ttggccaagc atagttcatg
     1921 tcaccacgaa gttgtttaca actcatcacg atgtgagaac caccgtaaga tttacgcaat
     1981 gtgattgtta ctttaggtac agtagcttca ccgtaagcat acagcagttt ggcaccatga
     2041 aggatgacgc cattgtattc ctgacctgtt cccggaagga atcccggtac gtctaccaac
     2101 gacacgatag ggatattgaa agcatcgcag aaacgaacga aacgtgcgcc tttgcgagat
     2161 gcgttgctgt cgagtacacc ggcaaggaat ttaggctgat tggctacaat acctacagac
     2221 tgaccgttga aacgggcaaa accgataatg atattcttgg catagtcttt ctggatttcc
     2281 aggaattcgc cgttgtctac gatagcgctg ataacctcgt acatatcata cggtttgttg
     2341 gggctgtcgg ggataatatc gttcagagaa tcttccagac ggtcgatcgg atctgtgcaa
     2401 tccacgtatg gagcttcttc gaggttgttt tgaggaatat agctcagaag tgtgcggatc
     2461 aatgccagac cttcttcttc tgttttggca gtgaaatgag taacacccga tttggtagag
     2521 tgaacgcttg ctccgccgag gttttcctgg cttacgtctt caccggttac agttttcact
     2581 actttcggac ctgtaaggaa catataagaa gtaccttcca tcatcagtgt gaagtctgtc
     2641 agtgccggag aataaacagc accgccggca caaggcccga agataccgga aatctgcgga
     2701 atgacacccg aagcgaggat attgcgctgg aagatttctg cgtaacctgc caaagcgtta
     2761 ataccttcct ggatacgtgc accgcccgag tcattgatac cgatacaagg agcacccatc
     2821 ttcattgcct gatccataat cttacagatc ttcagtgaca ttgtttccga cagtgaacct
     2881 gcagtaacgg taaagtcctg tgcaaaaaga tatacgagac gaccttcgat agtaccgcaa
     2941 ccggttacta caccatcacc cggatagtgc tttttctcca tgccgaagtt cgtgcatctg
     3001 tgctcaacga acatgtccat ttcttcgaag ctaccttcat ccaacagcat ggctatacgc
     3061 tcacgcgctg tgtatttccc tttttcatgt tgcttcgcaa ttgctttttc gccgcctccg
     3121 atgcgtgcaa cggcacggcg ttcaataagc tctttaattt tttcaagttg attactcatg
     3181 agttcttgtt gtttttttag agtttatgat tagtgttcgc agagttctgt cagcacaccc
     3241 agtgttgatt tcgggtgaag gaaagcgatg ttcagacctt cggcaccttt gcggggagcc
     3301 ttgtcaatca gacggatttc tttgctttct gcttctgcca aagcattagc tacgccatct
     3361 tctacggcaa atgctacgtg atgaacacct gcacctttgt tttcaataaa tttagcaatg
     3421 gtgctttcgg ggcaagtcgg ctccaacaat tcgatttttg tttcacctac ctttaaaaaa
     3481 gcggttctta ctttttgatc ttctactgtt tcgatgttgt aacatttcag acccaatacg
     3541 ttttcgtagt aaggaagggc ttcttcgatg ctttttacag caatgcccag atgctcaatg
     3601 tgtgaaatct tcataactat atttttttat atggtttaat tatgtgctta tatggcataa
     3661 aacagcacaa agaaagacat ttcttgtcta ataaagaaat atttctgcat ttaattattg
     3721 gggaataaag cataaaaaat gctccggatg ggtgacaaag acgggttttg taatcttgag
     3781 attacattta gacataaaac ttgaccagaa gattattcag ccatggccag ctgatacgga
     3841 accagcggcg ggtactgctt tgtttgcctc cgaaacggag tacgaaatca cgataaccat
     3901 gcttgcggaa gggaagtcct acatccatga actccatgtg acggaatccc cgttcatggg
     3961 catcttcaag tgctttccat actgccagta tgccgggtga ctgcagtgca taggtctttc
     4021 gcattcctcc tgaaaaccag ataaaagcat tttcgccgga atagatgcag acggagccgc
     4081 cgataatctt ttccttgtat tttacgatga atatttttgc ctgttttcct ctgataagca
     4141 tgctgttcat gtggcggaaa aagtcattgg cagggaaata tctccgtatt cgtgaagaat
     4201 aaactttgtg cagcatccgc gagaattcct taatctcttc caccgtatgg gcttctacta
     4261 ctttggctcc gtttttgagc cctttccgga tctgacggat acgtgatggg ctgaaacggt
     4321 cttccgcttt ctgcgtacta tgcagagagt tacggacacg cagccagttg accggaaaaa
     4381 agtcgttctt tcggaagaaa cggtaaccga acatggaatt atccagattg cggaattcaa
     4441 taagaatgca ggtgcgtgat gcttcttgtg tgagatgctc cagcatttca ccgaaaattt
     4501 cttcctcctt ctctttactt gccggtagag aagtgtccag aaattcacct gcaccatata
     4561 ccacacattg tttgacaaga gaggagggaa gccacttttt ggtcttgcgt atggcggcaa
     4621 gcaggcgtgc tacaggtctg ccttcttctg ttgccactat cagtagcggg gtatatcccg
     4681 gagtcgattc atagatctga aacaactcct tggaatgaaa agtattggtt cccggtaaat
     4741 caggaatgtc ttttccgcga taatatgttg ttagtttaag tggcatccaa atacaaatt
//
