LOCUS       gnlProkkaJFGECHMD_224   4781 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JFGECHMD_224.
ACCESSION   gnlProkkaJFGECHMD_224
VERSION     gnlProkkaJFGECHMD_224
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-18 00:12:13
            Original ID  :: gnl|Prokka|JFGECHMD_224
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4781
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..546
                     /NRPS_PKS="Domain: PKS_KR (19-109). E-value: 1.4e-08.
                     Score: 26.7. Matches aSDomain:
                     nrpspksdomains_ctg224_1_PKS_KR.1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     159.3; E-value: 2e-48)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg224_1"
                     /transl_table=11
                     /translation="MAAAVTAALNGWTEAHPDAYIEVLINNAGIRKDNLLLWMAGEEWH
                     RVLDISLSGFFNVTQPLLKDMLVKRFGRIVNIVSLSGIKGMPGQTNYSAAKGGIIAATK
                     ALAQEVAKKKVTVNAVAPGFIRTDMTGDLDEAELKKLVPAGRFGEPEEVADLVAFLVSP
                     AASYITGEVISINGGLYT"
     protocluster    1..4781
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[582:1782]"
                     /cutoff="20000"
                     /detection_rule="(APE_KS1 or APE_KS2)"
                     /neighbourhood="20000"
                     /product="arylpolyene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      583..1782
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(APE_KS1 or APE_KS2)"
                     /neighbourhood="20000"
                     /product="arylpolyene"
                     /protocluster_number="1"
     cand_cluster    1..4781
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(APE_KS1 or APE_KS2)"
                     /kind="single"
                     /product="arylpolyene"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..4781
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="arylpolyene"
                     /region_number="1"
                     /rules="(APE_KS1 or APE_KS2)"
                     /tool="antismash"
     aSDomain        58..327
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg224_1_PKS_KR.1"
                     /evalue="1.40E-08"
                     /label="ctg224_1_PKS_KR.1"
                     /locus_tag="ctg224_1"
                     /protein_end="109"
                     /protein_start="19"
                     /score="26.7"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="YIEVLINNAGIRKDNLLLWMAGEEWHRVLDISLSGFFNVTQPLLK
                     DMLVKRFGRIVNIVSLSGIKGMPGQTNYSAAKGGIIAATKALAQE"
     CDS_motif       235..285
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg224_1_0001"
                     /evalue="2.50E-05"
                     /label="PKSI-KR_m4"
                     /locus_tag="ctg224_1"
                     /protein_end="95"
                     /protein_start="78"
                     /score="16.8"
                     /tool="antismash"
                     /translation="SLSGIKGMPGQTNYSAA"
     CDS             583..1782
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (70-397). E-value:
                     1.1e-51. Score: 168.0. Matches aSDomain:
                     nrpspksdomains_ctg224_2_PKS_KS.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     arylpolyene: APE_KS1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 289.5; E-value:
                     7.3e-88)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg224_2"
                     /sec_met_domain="APE_KS1 (E-value: 1.3e-157, bitscore:
                     513.5, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGIYSCIGKNLEEVKTSLYKGKSGIGIDPVRKEFGYRSALTGLLD
                     KPNLKGVLDRRQRIGLPEQGEYAYLATAEAFAQAGIDEEYLLNHEVGILYGNDSSTAPV
                     INAVDIIREKRNTALVGSGSIFQSMNSTVTMNLSVIFKLRGVNFTISGACASGSHAIGM
                     GYFLIRSGIQDCIVCGGAQEVNMYSVGSFDGLAAFSVREDEPGKASRPFDKHRDGLVPS
                     GGGATVIIESYESAKRRGANILAEVVGYGFSSNGEHISVPNVDGPRRSLEMALKDSGLA
                     ARDIQYVNAHATSTPLGDLNEAKAIAAVFGETSPYVTSTKSMTGHEMWMAGASEVIYSM
                     LMMQNGFIAPNLNFTEPDEASALLNIPAETKQLEFDTFLSNSFGFGGTNSTLIIRKFKS
                     "
     aSDomain        793..1773
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg224_2_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="1.10E-51"
                     /label="ctg224_2_PKS_KS.1"
                     /locus_tag="ctg224_2"
                     /protein_end="397"
                     /protein_start="70"
                     /score="168.0"
                     /tool="antismash"
                     /translation="ATAEAFAQAGIDEEYLLNHEVGILYGNDSSTAPVINAVDIIREKR
                     NTALVGSGSIFQSMNSTVTMNLSVIFKLRGVNFTISGACASGSHAIGMGYFLIRSGIQD
                     CIVCGGAQEVNMYSVGSFDGLAAFSVREDEPGKASRPFDKHRDGLVPSGGGATVIIESY
                     ESAKRRGANILAEVVGYGFSSNGEHISVPNVDGPRRSLEMALKDSGLAARDIQYVNAHA
                     TSTPLGDLNEAKAIAAVFGETSPYVTSTKSMTGHEMWMAGASEVIYSMLMMQNGFIAPN
                     LNFTEPDEASALLNIPAETKQLEFDTFLSNSFGFGGTNSTLIIRKF"
     CDS             1801..2046
                     /NRPS_PKS="Domain: PP-binding (8-75). E-value: 2.1e-11.
                     Score: 36.1. Matches aSDomain:
                     nrpspksdomains_ctg224_3_PP-binding.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg224_3"
                     /sec_met_domain="PP-binding (E-value: 2.7e-12, bitscore:
                     36.1, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASTELIQKINEKLSEEFEIDIELIQPDAPLMQTLELDSLDLVDM
                     VVLVEHNFGFTLKAQDFIGIKTFQDFYDFIDSRVNG"
     aSDomain        1825..2025
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg224_3_PP-binding.1"
                     /evalue="2.10E-11"
                     /label="ctg224_3_PP-binding.1"
                     /locus_tag="ctg224_3"
                     /protein_end="75"
                     /protein_start="8"
                     /score="36.1"
                     /tool="antismash"
                     /translation="KINEKLSEEFEIDIELIQPDAPLMQTLELDSLDLVDMVVLVEHNF
                     GFTLKAQDFIGIKTFQDFYDFI"
     CDS             2039..2938
                     /locus_tag="ctg224_4"
                     /transl_table=11
                     /translation="MGDSNDTGWKGKTRGGSFGYLFFILLIKGLGVKAAYAFLCLVVVY
                     FVPFAPKATRAIWKYARQRLGHSRFRSACFLLKNYYRLGQTLIDKVAVGAGMAKQYTFD
                     FGAQYPGFLEVLNGNEGVVMIGAHVGNWEIGAPFFDDYGKKMNIVMFDAEYQKIKELLE
                     KNTNGKNYKVIPVNEDNLTHIFRIRDVLANGEYVCFQGDRYVEGAQTFKETFLGAEASF
                     PAGPFLLASRLGVPVVFYFAVREKGMHYKFNFVLAQPEKRVKGVVPGQGLLKQYVSALE
                     SVVKQYPEQWFNYYDFWK"
     CDS             2959..4044
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042:O-methyltransferase (Score: 133.5; E-value:
                     1.7e-40)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg224_5"
                     /transl_table=11
                     /translation="MELSSPQKEIYAKDRFSAVQAQRLAQEIAFGPVVFQVSRLMVKFG
                     IFGMLREAQDGLTMDEIAEKTGLSHYAVQVLLESSLTIGTILLKDGHFHLGKAGWFLLN
                     DEMARVNMDFNHDVNYLGLFRLEEALLNGKPEGLKVFGNWPTIYEGLSSLPEKVQASWF
                     GFDHFYSDNSFKQALEIVFANPVRTLLDVGGNTGRWAMQCVAYSPEVEVTIMDLPQQLE
                     MMREQTRELEGAARIHGHAADLLDDGTSFPQPFDAIWMSQFLDCFSEEQVLSILSRAAR
                     SMEAGSRLYIKETFWDRQRFETASYCLAQTSLYFTAMANGNSKMYYSADMARLVEKAGL
                     AIEQIHDGLGLGHSIMQCKLK"
     CDS             4047..4496
                     /locus_tag="ctg224_6"
                     /transl_table=11
                     /translation="MASISFDTPLLSGDGLLDLIPQRAPIVMVDRFFGMDDYGSYTGLE
                     VEDSNLFCQDGQLDECGITEHIAQSAAIRVGYLYRCAGEKIPVGFIGSVNKMNFYALPQ
                     VGDELHTTIRVEQEIFDITLISATVQVNGTTVADGQLKIFLKKDA"
     CDS             4571..4780
                     /locus_tag="ctg224_7"
                     /transl_table=11
                     /translation="MEVVWHGEYVRYMEDGREAFGRQYGIGYTEIRDAGYVVPIVELNI
                     QYKQSLKYGESAIVETRYIRTEAAK"
ORIGIN
        1 gatgccgcag cagtgactgc tgcccttaac ggatggacgg aagcccatcc ggatgcctat
       61 atcgaagtgc tgatcaataa cgcaggcatt cgcaaagata acctcctgct ctggatggcc
      121 ggagaggagt ggcaccgtgt gctcgatatc agcctgtcgg gtttctttaa cgttacgcag
      181 cctttattga aagacatgct ggtaaaacgt ttcgggcgga ttgtcaatat cgtttccctg
      241 tctgggataa aggggatgcc gggacagacc aattattcgg cggctaaagg tggtattatt
      301 gcggcaacca aagcgctggc gcaggaagtt gccaagaaga aggtcacggt aaatgctgtt
      361 gctcccggtt ttatccgtac ggatatgacc ggcgatctgg atgaagcgga gctgaaaaaa
      421 ctggtgcctg caggccgctt cggcgaaccg gaagaggtgg ctgacctggt agcttttctt
      481 gtttcgcctg ccgcctcgta tattactggc gaagtgattt caataaacgg aggattatat
      541 acctgataat aaagatatat gaatagagtt gttattacag gaatggggat ttactcctgc
      601 atcggaaaga acctggagga ggtaaagact tcattatata aagggaaatc gggtatcggt
      661 atcgatccgg ttcgcaaaga attcggatac cgctcggcat taacgggact attggataaa
      721 ccgaacctga aaggcgtact ggatcgccgc cagcgtatcg gtcttcccga acagggcgaa
      781 tatgcttatc tggcaacggc tgaggctttt gcacaggcgg ggatagacga agagtatctg
      841 ctgaaccacg aagttggtat cctttacgga aacgacagca gtacggctcc tgtgatcaat
      901 gcggtggata tcatccgtga gaaaagaaat acggcgctgg tcggttccgg ctctattttt
      961 cagagcatga attcgacggt gacgatgaac ctgtcggtta ttttcaagct gcggggtgtc
     1021 aactttacga tctccggtgc gtgtgccagc ggttctcatg ccattgggat gggatatttc
     1081 ctgatccgtt cgggtataca ggattgtatc gtatgtggcg gggcgcagga agtgaatatg
     1141 tattcggtcg gcagtttcga cgggctggcg gctttctctg tcagggagga cgagcccgga
     1201 aaggcatcgc gcccgtttga caaacaccgt gacggattgg ttcccagtgg gggaggggcg
     1261 accgttatca ttgaaagtta cgagtcggct aaacggcggg gggcgaatat cctggcggaa
     1321 gtggttggct atggcttttc ttccaacggc gaacatattt ccgtcccgaa cgtagacggg
     1381 ccacgccgct cactcgaaat ggcattgaaa gattcgggat tggctgcccg cgatatacaa
     1441 tatgtaaatg cacatgctac atccactccg ctgggcgacc tgaatgaggc gaaggcgatt
     1501 gctgccgtct tcggagaaac atccccgtat gtcacctcta ccaaatcgat gaccgggcat
     1561 gagatgtgga tggccggggc gagcgaagtg atctattcca tgctgatgat gcagaacggg
     1621 tttatcgctc ccaatctaaa ctttacggaa cccgacgagg cttctgcctt gttgaacatt
     1681 ccggcggaaa cgaaacaact ggagttcgat acgttcctgt ccaactcgtt tggctttggg
     1741 ggaaccaact ctacattaat aatcaggaaa ttcaaatctt aacagacaat aaacaaacaa
     1801 atggcaagca cagaattaat tcaaaagatc aacgagaaac tatcggagga gttcgaaata
     1861 gatatagaac tgatacaacc cgatgccccg ttgatgcaga cactcgaact cgatagcctc
     1921 gacctggtgg atatggtcgt actggtagag cacaacttcg gttttaccct gaaagcgcag
     1981 gactttatcg ggattaaaac gttccaggac ttctacgact ttattgactc acgtgtgaat
     2041 gggtgatagt aacgatacgg gctggaaagg taaaacgcgc ggaggttctt tcggttacct
     2101 cttcttcatt ctcttgataa aagggctggg ggtaaaggcg gcgtatgcgt ttctttgtct
     2161 ggtcgtggtc tattttgtac cgtttgcccc caaggcgacc cgtgcgatct ggaaatatgc
     2221 gcggcaaagg ctgggtcaca gccgtttccg ttccgcctgt ttcctgttga agaattatta
     2281 ccggttggga caaaccctga tcgataaagt ggcggtcggt gccgggatgg ctaaacaata
     2341 tacttttgac ttcggcgcgc aatatcccgg ttttcttgaa gtattgaacg ggaacgaggg
     2401 agtagtgatg atcggtgccc atgttggcaa ctgggagatc ggtgcccctt tcttcgacga
     2461 ttacgggaag aagatgaata ttgtgatgtt cgatgccgaa tatcagaaga taaaagaatt
     2521 actggagaag aatacgaacg gaaagaacta caaggttatc cctgtaaatg aagataatct
     2581 gacacatata ttccggatca gggatgtcct ggccaacggg gaatatgttt gtttccaggg
     2641 agaccgttat gtggagggtg cgcagacttt taaggaaact tttctcggtg cggaagcctc
     2701 ttttccggcg ggcccttttc tgctggcatc ccgcctgggg gtacctgtcg tattttattt
     2761 cgctgtgcgt gagaaaggaa tgcactataa gtttaacttt gtactggcgc aaccggagaa
     2821 gcgcgtcaaa ggggtggttc ccggacaggg attgctgaaa cagtatgtct cagcgttgga
     2881 atcggttgtg aaacaatatc cggaacaatg gtttaattac tatgacttct ggaaataaca
     2941 gatagaataa gagagaacat ggaattatca tctcctcaaa aagaaatata cgcaaaggat
     3001 cgtttttcgg ctgtacaggc gcaacgtctg gcacaggaga tcgcttttgg cccggtagtc
     3061 ttccaggtgt cgcgcctgat ggttaaattc gggatcttcg gaatgctccg cgaagcacag
     3121 gacggtctga cgatggatga gattgctgaa aagacaggcc tttcccatta tgccgtacag
     3181 gtgttgttgg aatcgtcgtt gacgatcggt acgatcctgt tgaaagacgg gcatttccat
     3241 ctgggaaaag ccggatggtt cctgctgaac gatgaaatgg cccgggtcaa tatggatttc
     3301 aaccacgatg tgaactatct gggactgttc cggttggaag aggctttgct gaatggcaag
     3361 cccgaaggcc tgaaggtttt cggtaactgg ccgaccatct acgagggctt gtccagcctg
     3421 ccggaaaagg tgcaggcgag ctggttcggt ttcgaccatt tctattccga caactccttt
     3481 aagcaagctc tggagattgt ttttgccaat ccggtgcgta cgttgctgga tgtaggcggc
     3541 aataccgggc gttgggcgat gcaatgcgtg gcttattcgc cggaggtgga agtgaccatt
     3601 atggatctgc cccagcagtt ggagatgatg cgtgaacaga cccgggaact ggaaggggct
     3661 gcccgtattc atggtcatgc agcggatttg ttggacgacg gaacaagttt cccgcagcca
     3721 ttcgatgcca tctggatgag ccagttcctc gattgtttct cggaggagca ggtactgagc
     3781 atcctgtcac gtgccgcccg gtcgatggaa gcaggcagcc gcctgtatat caaggagact
     3841 ttctgggatc gtcagcgttt cgagaccgct tcttattgcc tggcgcagac tagcctttat
     3901 tttacggcaa tggctaacgg caacagcaaa atgtattatt ccgctgatat ggcccgcctg
     3961 gtagaaaagg ccggactggc gatcgaacag atacatgacg gcctggggct gggacatagt
     4021 attatgcaat gtaaactgaa atagatatgg catcaatctc gttcgatacc cctttactgt
     4081 ctggtgacgg gctactggat ctgatcccgc agcgtgcgcc gatcgtgatg gttgaccgtt
     4141 tcttcgggat ggatgactac gggtcttata ccgggttgga ggtagaggac tcgaacctgt
     4201 tttgccagga cggccaactg gatgaatgcg gcataacgga acatatcgcc caatcggctg
     4261 ccataagggt gggctatctc tatcggtgtg cgggcgaaaa gatcccggtc ggttttatcg
     4321 gctcggtgaa taagatgaac ttctatgctc tgccgcaggt gggggacgaa ttgcatacca
     4381 cgatccgggt agagcaggag atttttgata taacgcttat ctcggccacc gtacaggtga
     4441 atggcactac ggttgccgat ggtcagctta agatatttct aaagaaagac gcatgagacg
     4501 gatgaaaata caggaagcgg cattgaccaa ccgcacgact ttgcgggtca ggttcagcga
     4561 ggtagattcg atggaagtcg tttggcatgg cgaatatgtc cgctatatgg aagacgggcg
     4621 ggaagctttc ggtcgccagt acggtatcgg ctatacggag atacgggatg ccggctatgt
     4681 agttccgatc gtcgaactga acatccagta taagcaatcg ctgaaatacg gtgagtcggc
     4741 tattgtcgag acacggtata tccggacgga agcggctaag a
//
