LOCUS       c00195_gnlPro..         5114 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JFGECHMD_195.
ACCESSION   c00195_gnlPro..
VERSION     c00195_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 02:34:20
            Original ID  :: gnl|Prokka|JFGECHMD_195
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5114
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5114
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2887:5114]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2888..5114
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5114
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5114
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..1076)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg195_1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 9.3e-20, bitscore:
                     59.4, seeds: 65, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDSSMRNQLSGIFSGLTNEYIFDIQVSPEHESRKELLELLNDVA
                     SCSDKITVQVADGEGLEFSLLKNGEKTGIKFRGVPNGHEFTSLLLAILNSDGKGKNIPD
                     ESICNRVKALNGPVKLTTYVSLTCTNCPDVVQALNAMATLNPEISHEMVDGAINQEEVA
                     AMKVQGVPSVFADGKLIHVGRGDFGELLGKLEAQYGVKEENLPVVEKNYDVIIVGGGPA
                     GSAAAIYTARKGLAVAVVADRIGGQVKETVGIENMISVSQTTGQQLALDLMSHMKDYPI
                     DILEHRRIEKVEVEGREKLLTTSTGEKLIAPAVIVATGASWRRLNVPGESEYIGRGIAF
                     CPHCDGPFYKGKHVAVVG"
     CDS             complement(1170..1736)
                     /locus_tag="ctg195_2"
                     /transl_table=11
                     /translation="MEPIINSQAPEFKVQAYHNGSFKTVSSDDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYDQFQAMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGV
                     LSRAFGVMIEEDGMAYRGTFLVNPEGKIKLAEINDNGIGRNADELLRKVEAAQFIAEHP
                     SEVCPAKWKKGGETLKPSIDLVGKI"
     CDS             complement(1897..2799)
                     /locus_tag="ctg195_3"
                     /transl_table=11
                     /translation="MEQHHHDHHHHEITSLNKAFIIGISLNLAFVVAEFTAGFWYNSLG
                     LLSDAGHNLGDVASLVLAMLAFRLAKVHPTCSYTYGYKKSTVLVSLLNAVILLVAVGII
                     IAESIGKLLHPRPVEGDAIAWVAGIGVVINALTAWLFMKDKDKDLNVKGAYLHMAADAL
                     VSVGVVVSGIVIAHTGWYIIDPIIGLLIAFVIIVSTWGLLRDSIRLSLDGVPAGTDIDK
                     VEKTIHSVEGVEGVHHLHIWAISTTETALTAHVSVADISEMERIKQEIKHDLQHVGIQH
                     ATLEFETSGFKCDGHCCSD"
     CDS             complement(2888..3616)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72; E-value:
                     2e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg195_4"
                     /sec_met_domain="Radical_SAM (E-value: 7e-36, bitscore:
                     112.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LEIKGNIHSLESFGTVDGPGIRFVIFMQGCPLRCLYCHNPDTWDS
                     KGLAKYQMTPEELLAEVLRYKNFIAKGGVTVTGGEPLLQAEFLREFFRLCREAGIHTAL
                     DTSGYICTPKALAVLEYADLVLLDIKTIDPDLHPRLTAVKLDNTLRFLDELEKRGVPVW
                     IRHVIVPELTDNDEALDKLAEYISHYTVVQKAELLPYHTMGTYKYEAQGLDYKLKEVEP
                     LSAERLANAKEIFKKHGITI"
     CDS             complement(3624..5114)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg195_5"
                     /sec_met_domain="Gly_radical (E-value: 7e-46, bitscore:
                     143.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4e-180, bitscore:
                     588.5, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="YLGYLAAVKDQDGAAMSLGRTSTFLDIYIEKDIREGKLTEEEAQE
                     LIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSLVTKTSYRYLHTLYN
                     LGPAPEPNLTVLWFNNAPENWKRFCAKVSIDTSAIQYENDDLMRPDYGDDYGIACCVSP
                     MKIGKQMQYFGARANLAKCLLYAINGGRDERSGVQVAPRFEPITSEYLDYNEVMEKYEQ
                     MMRWLAKVYVNALKIIHYMHDKYAYEAFEMALHDGSVERIRATGIAGLSIVADSLAAIR
                     DTKVKVIRDERGLAVGFEREGDYVPFGNNDDRTDSIAVDITEKFMEYLRQHQTYRNATP
                     TQSILTITSNVVYGKKTGATPDGREGGTPFAPGANPMNGRDTKGAVAALASVAKLPFQH
                     AHDGISYTFAVSPATLGKERDIQIANMVGLLDGYFTPDGGQHLNVNVFDKELLLDAMEH
                     PENYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
ORIGIN
        1 ccgcctacaa cagccacatg cttgccttta tagaacggac cgtcgcaatg cggacagaaa
       61 gcgataccgc gtccgatata ttcggattct ccggggacat tcaacctacg ccagctagct
      121 ccggttgcta cgataacggc cggggctatc agtttttcgc cggtagaggt tgtcagcagc
      181 ttttcgcgtc cttcgacttc caccttctcg atacggcggt gttcaaggat atcgatcgga
      241 taatctttca tatgcgacat caggtcgaga gccagctgct gccccgtcgt ctgcgaaaca
      301 gagatcatat tctcgatacc gacagtttcc ttcacctgtc cgccgatacg gtcggccaca
      361 acagccaccg ccaaaccttt acgggcagta tagatagctg ccgcacttcc ggcaggacct
      421 ccgccgacga tgataacatc gtagttcttt tctaccaccg gcaggttctc ctctttcacg
      481 ccatactgcg cttcgagttt gccaagcagt tcgccaaagt ctccacgacc tacatggatc
      541 agtttaccgt cggcaaatac ggaaggtact ccctgcactt tcatggcagc aacctcttcc
      601 tggttgatcg caccgtccac catttcgtga gagatttccg ggttcagcgt agccatggca
      661 ttcaaagcct gtacaacgtc aggacagttg gtacaggtca aagaaacata tgtagtcagt
      721 ttgactgggc cattcagggc cttcacacgg ttacaaatac tttcgtcggg aatatttttg
      781 cctttgccgt cgctgttcag gatagccagc agcaacgaag taaattcgtg tccgttggga
      841 acgccccgga atttaatacc ggttttttcc ccgtttttca gcagagagaa ctccaggcct
      901 tcgccgtctg ccacctgtac ggtgatctta tcggaacagg aagccacatc gttcagcaat
      961 tccaataatt ccttcctgct ttcatgttca ggagaaactt gtatatcaaa tatatattcg
     1021 ttcgtcaatc cgctaaagat accggacaac tgatttctca ttgatgaatc taacatagtt
     1081 gaatccttta ttttaattat gaatagaaca actgatattt gtttagataa aaaaggccga
     1141 caatatgttg ccggcctcca gttttactat tagatcttac cgaccagatc gatgctcggt
     1201 ttcaatgttt cgccaccttt tttccatttg gcggggcaaa cttcgctcgg atgttcggcg
     1261 atgaactggg cagcttctac tttacgaagc agttcgtcgg cgttacggcc gataccgtta
     1321 tcgttgattt ctgccagttt gatctttcct tccggattta ccaggaatgt accgcggtaa
     1381 gccataccgt cttcttcgat cattacaccg aatgcacggc tcaatacgcc tgtcgggtct
     1441 gccaacatcg ggtatttgat cttacgaatc gtttcagaag catcgtgcca tgctttgtgt
     1501 acgaagtgtg aatctgtact tacagagtaa acttctacac ccattgcctg gaactggtca
     1561 tatttgtcag ccatatctac caactcggtc ggacaaacaa atgtaaagtc agccgggtag
     1621 aaaaagaaaa tcgcccattt gcctttgatg tcgtcgctgc ttacagtttt gaaactaccg
     1681 ttgtggtatg cctgaacttt aaactcgggt gcttgtgaat tgataatagg ttccatagaa
     1741 tttttatttt aatcgttatt actattttgt ttttgttact gatgcaaaga taagttgccg
     1801 attcgaggta agaacaaaaa atccaatcga aaggttttat gcagtcatag acaaaatctt
     1861 tgagtatcag taaatcaggt ttataatata catgtttcag tctgagcaac agtgcccatc
     1921 gcacttgaat cccgacgttt caaattcgag cgtagcatgc tggattccca catgctgaag
     1981 gtcgtgtttt atctcctgtt tgatacgttc catttctgaa atatccgcaa cggaaacatg
     2041 ggcggtaagt gccgtttcgg tcgtactgat cgcccagata tggagatgat ggacgccttc
     2101 aacgccttcg accgaatgta tcgtcttttc caccttatcg atgtccgttc ccgccggcac
     2161 accgtccaga gagagacgga tactgtcgcg cagcagtccc caggtagaaa caatgataac
     2221 gaacgcaatc aacaggccga taatcgggtc gatgatatac catccggtat gggcaataac
     2281 aattccggag acaaccacgc cgaccgatac cagtgcatcg gcagccatat gcaagtaagc
     2341 acctttcaca ttcaagtctt tatccttatc cttcatgaaa agccatgccg taagtgcatt
     2401 tataacgact ccgatacctg ccacccaagc gatcgcatca ccctcgaccg gacgcggatg
     2461 cagcagtttt cctatacttt cagcaataat aatcccgacg gccaccaaga ggatgaccgc
     2521 attcagaagg gatacgagta cagtgctttt cttatatcca taggtatagc tgcaagtcgg
     2581 gtgcacctta gccagccgga aagccagcat agccagtacc aggctggcca catctcccag
     2641 gttatgccct gcgtccgaca gaagtcctaa tgaattatac cagaagccag ccgtaaattc
     2701 ggctacgaca aaagccaggt tcagggagat acctataatg aaagccttgt tcaaagaggt
     2761 tatttcgtgg tggtgatgat catgatgatg ttgttccata ttcattccta cttttatttg
     2821 agtaacaata tgggacaaaa ctatgttttt tagagcaata aaaatatacc tgattatagg
     2881 taaattatca gatggttata ccgtgcttct taaatatttc tttggcattt gccagacgtt
     2941 cggccgataa aggttctacc tctttcagtt tgtagtcgag accttgagcc tcgtatttat
     3001 atgtgcccat cgtgtgataa ggcaagagtt ccgccttctg tacaaccgta tagtggctga
     3061 tgtattcggc cagtttatcc aaggcttcgt cattatccgt cagttcgggc acaatgacat
     3121 ggcgtatcca aaccgggaca ccgcgttttt ccagttcgtc cagaaaacgt aacgtattat
     3181 ccagtttcac agccgttaga cggggatgca ggtcgggatc gatggttttt atatccaata
     3241 agaccaggtc ggcatattcc agaaccgcca gggctttcgg tgtgcagata tagccggatg
     3301 tatcgagcgc cgtatgaata ccggcttccc ggcaaagacg gaaaaactcc ctgagaaatt
     3361 cagcttgcaa taagggttct cccccggtga cagtcacgcc acccttggcg ataaagttct
     3421 tatatcgtaa aacctcagcc aataattctt caggcgtcat ctgatatttg gcaagccctt
     3481 tgctatccca ggtatccgga ttgtggcaat acaggcaacg gagcggacac ccctgcataa
     3541 agataacaaa acgaattccg ggaccatcaa cggtcccgaa actctctagt gaatgtatgt
     3601 ttcctttaat ttccaacttt caattacaaa gaatgattga ttgttcttga aatcacatcc
     3661 agctgctgtt cgcgtgtcag tttcacaaag tttactgcat aacccgatac acggattgtc
     3721 aactgcggat agttttccgg atgctccata gcatcgagca acagctcctt gtcgaatacg
     3781 ttcacattca gatgctgtcc gccgtcagga gtaaagtaac cgtccagcag gccgaccata
     3841 ttggcgatct ggatatcacg ttctttccct aacgttgcag gagaaacagc gaatgtataa
     3901 gagataccgt cgtgtgcatg ctggaacggc agcttagcga cagaagccag tgcagcaaca
     3961 gcgcctttgg tatcacgtcc gttcatcggg ttcgcacccg gagcaaacgg cgtaccgcct
     4021 tcgcgaccgt cgggagttgc acctgttttc tttccatata cgacattaga cgtaatagtc
     4081 aggatagact gtgtcggagt ggcgttgcgg tatgtctggt gctgacgcag atattccatg
     4141 aacttttcag tgatatcgac agcaatacta tctgtacgat cgtcattgtt accgaaagga
     4201 acataatcgc cttcacgttc gaaaccgaca gccaggccac gttcgtcgcg gatcactttt
     4261 actttcgtat cgcggatagc agccaatgag tcggcaacga tagaaaggcc ggcaatacct
     4321 gttgcgcgga tacgttctac cgaaccgtca tgcaaagcca tctcgaatgc ttcatatgca
     4381 tatttatcgt gcatgtaatg gatgatcttc aatgcgttca catagacttt agccagccag
     4441 cgcatcatct gttcgtattt ctccatcact tcgttgtaat ccaggtattc gctggtgatc
     4501 ggttcaaacc tcggagccac ctgtacacct gatctttcat cgcgccctcc gttgatggca
     4561 tacagcaaac atttagccag gttggcacgt gcgccgaaat actgcatctg cttaccgatc
     4621 ttcatcggag acacgcagca ggcaatacca taatcatctc cataatccgg acgcatcaga
     4681 tcgtcgttct catactggat agcggaagta tcgatagaaa ccttcgcaca gaaacgtttc
     4741 cagttttcag gagcgttgtt gaaccacagt accgttaagt tgggttccgg tgccgggccg
     4801 aggttatata atgtatgcag ataacggtaa gacgtcttcg tcaccaatga acggccgtcg
     4861 acaccctgtc cgcccaaaga ctcggttacc caaacagggt caccggagaa caggtcgtta
     4921 tattccggag tacgaaggaa acggacgata cgcagtttga taataaactg atcgatcagc
     4981 tcctgagctt cttcttccgt cagcttgcct tcacggatat ctttttcaat ataaatatcg
     5041 aggaaagtag aagtacgtcc caacgacatg gcagccccat cctggtcctt caccgcagcc
     5101 agataaccca gata
//
