LOCUS       c00258_gnlPro..         3976 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IMPIMKPL_258.
ACCESSION   c00258_gnlPro..
VERSION     c00258_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 03:15:37
            Original ID  :: gnl|Prokka|IMPIMKPL_258
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3976
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..582)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: ECH_1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg258_1"
                     /sec_met_domain="ECH_1 (E-value: 1.9e-62, bitscore: 198.9,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTQREWTTIREYDDILFDYYNGIARITINRERYRNAFTPTTTAE
                     MSDALRICREEADIDVIVITGAGDKAFCSGGDQNVKGRGGYIGKDGVPRLSVLDVQKQI
                     RSIPKPVIAAVNGFAIGGGHVLHVVCDLSIASENAIFGQTGPRVGSFDAGFGASYLARV
                     VGQKKAREIWFLCRKYNAQEALDMGLVNKVV"
     protocluster    1..3976
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:3975]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..3975
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..3976
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /kind="single"
                     /product="TPP_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3976
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_fatty_acids"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /tool="gutsmash"
     CDS             complement(586..2253)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg258_2"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.5e-08, bitscore:
                     22.7, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYTDKKNILQLVALLEAHGITKVVLCPGSRNTPIVHTLSNHPNFT
                     CYPVTDERSAGYFAIGLALNGGKPAAVCCTSGTALLNLHPAVAEAFYQNVPLVIISADR
                     PAAWIGQMDGQTVPQPGVFQTLVKKSVNLPEIHTEEDEWYCNRLVNEALLETNHHGKGP
                     VHINVPISEPLFQFTADSLPEVRVITRYQGLNVYDRDYNDLVDRMSKYQKRMIIIGQMN
                     LIYLFEKRYIKLLYKHFAWLTEHIGNQTVPGIPVKNFDAALYAMPEEKVDQMVPELLIT
                     YGGHVVSKRLKKFLRQHPPKEHWHVSPDGEVVDLYGSLTTVIEMDPFEFLEKIASLLDN
                     RTPEYPRVWENYCKTIPEPEFAYSEMSAVGALLKSLPESCALHLANSSVVRYAQLYSIP
                     STIEVCCNRGTSGIEGSLSTAVGYAAASDKLNFIAIGDLSFFYDMNALWNVNVRSNLRV
                     LLLNNGGGEIFHTLPGLDMSGTSHKFIAAVHKTSAKGWAEERGFLYLQAQNDEELAEAM
                     KTFTQPEAMEQPVLLEVFTNKNKDARMLKNYYHQLKQK"
     CDS             complement(2273..3388)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Chorismate_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg258_3"
                     /sec_met_domain="Chorismate_bind (E-value: 3.3e-58,
                     bitscore: 185.3, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDEEISNLTTIDAFIQRKQPFAVYRIPGEKVPRLLTQAEGAVRL
                     IYDLKELNGQRGFVIAPFQVSESCPVVLIQPDQWGQPLLVDDDTEEDREVALRLQGQES
                     FLTSSTEEYAACFHAFINALRDRTFDKLVLSRHLTVDKVADFSPLSIFRAACKRYIHSY
                     IYLCYTPQTGIWLGSTPEIILSGEKDEWHTVALAGTQPLQDGRLPQVWDEKNRKEQDYV
                     ASYIRRQLLSLDIHATENGPYPAYAGALSHLKTDFRFALKDNKGLGDLLKVLHPTPAVC
                     GLPKEEAYQFILQNEGYDRRYYSGFIGWLDPEGRTDIYVNLRCMHIEDKQLTLYAGGGL
                     LASSELNDEWLETEKKLQTIKRLIAVPPLKS"
     CDS             complement(3385..3975)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: HBT"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg258_4"
                     /sec_met_domain="HBT (E-value: 1.5e-19, bitscore: 58.4,
                     seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIAVGDGVCDVTMIQLAGLGVAMGHSQDSVKACADYVTASNEEDG
                     VAVAVEKAIIAEVRAAEIPLDQLNAQARHALMGNLGIQYTYADEDRVEATMPVDHRTRQ
                     PFGILHGGATLALAETVAGLGSMILCQPDEIVVGMQVSGNHISSAHEGDTVRAVGTVVH
                     KGRSSHVWNVDVFTSTNKLVSSIRVVNSVMKKR"
ORIGIN
        1 aactacttta ttcactaatc ccatgtccaa tgcttcttgt gcattatact tccggcagag
       61 gaaccagatt tcgcgtgctt tcttctgacc gaccacacgt gccagataag acgcaccgaa
      121 tccggcatca aagctaccta cacgaggccc cgtctgaccg aaaatagcat tttccgacgc
      181 aatggacaaa tcacatacca catgaagcac atgtccgcca ccaatagcaa aaccgttcac
      241 ggcagcaatc accggtttcg ggatgctgcg aatctgcttc tgcacatcca atacgctaag
      301 acgaggcaca ccgtcttttc cgatatatcc gccacgtcct ttcacattct ggtcgccacc
      361 ggaacagaat gccttatcac cggctccggt aattacaatc acatcaatat ccgcttcttc
      421 acggcagatg cgcaatgcgt cactcatttc agcagtagtt gtcggagtaa acgcattccg
      481 atagcgttca cggttgatcg tgatacgggc aattccatta taataatcaa aaagaatatc
      541 gtcatattct ctgatggttg tccactctct ttgtgttgac ataatctatt tttgttttaa
      601 ttgatgataa taattcttca acatgcgcgc gtccttgttt ttgttggtaa acacctccaa
      661 gagcacaggc tgttccatag cttccggttg agtgaaagtt ttcatcgctt cagccagttc
      721 ttcgtcattt tgtgcttgca gatagaggaa tccacgttcc tctgcccaac ctttggccga
      781 tgttttatga actgccgcaa taaatttatg tgaggtaccc gacatatcca atcccggcaa
      841 ggtatggaaa atctctcctc ctccattatt taacagaaga acacgcagat tggagcgaac
      901 attgacgttc cacaatgcat tcatatcata gaaaaagctc aaatctccga tagctataaa
      961 gttcagtttg tcggaagctg ccgcataacc gacagccgta gacagcgagc cttcaatgcc
     1021 gcttgtcccc cgattacaac aaacttcaat cgtagaagga atggaatata attgcgcata
     1081 acgcaccacc gaactgtttg ccagatgcaa cgcacatgac tccggtaagg atttcaataa
     1141 cgcgccgacg gcagacattt ccgaataggc aaactccggt tcggggatgg tcttacaata
     1201 gttttcccac acacggggat attcgggagt ccgattgtcc agcaaactgg ctatcttctc
     1261 cagaaactcg aacgggtcca tttcgatcac tgtagttaat gagccgtaca aatcgactac
     1321 ctcaccgtcc ggcgatacgt gccaatgttc tttaggagga tgctgacgga gaaacttctt
     1381 caatcgtttg gaaacgacat gtcctccata agtaatcagc aattcgggaa ccatctgatc
     1441 taccttttct tcgggcatag cataaagtgc tgcatcgaag ttctttaccg gaataccggg
     1501 aactgtctgg ttgccgatat gttccgtcaa ccaggcaaaa tgtttatata ataacttgat
     1561 atatcttttt tcaaacagat agataagatt catctgaccg atgataatca tacgcttctg
     1621 gtatttactc atccggtcga ccaaatcgtt ataatcacga tcgtagacgt tcaacccctg
     1681 ataacgggta atgacacgca cttctggaag cgaatcggcc gtaaactgga acaatggttc
     1741 agaaataggg acgttgatat gtaccggtcc ttttccatga tggttcgttt ccagcaaagc
     1801 ttcattcacc agacgattgc aataccattc atcttcttcc gtatgaatct ccggaaggtt
     1861 taccgacttc ttaaccaatg tctgaaatac gcctggttgt ggtactgtct gtccatccat
     1921 ctgcccgatc caggcagcgg gacgatccgc agaaataatg accagaggaa cattttgata
     1981 aaatgcttct gccacagccg gatgaaggtt taataatgca gtaccggaag tgcaacagac
     2041 agcagcaggc ttaccaccat tcaacgcaag accgatggca aaatagccgg cactccgttc
     2101 atccgtcacc ggatagcagg taaaattcgg atggttagac aaagtatgta ctatgggcgt
     2161 attacgacta cccggacaca acacaacctt agtaatccca tgcgcctcaa gcaacgcaac
     2221 caactggagt atgttcttct tgtctgtata catgatgata attattagta gtttatgatt
     2281 ttaaaggagg cactgcaatc agccgtttga tggtttgcaa cttcttctcc gtttccagcc
     2341 attcgtcatt cagttccgaa gaggccaata atccaccgcc ggcataaaga gtgagttgct
     2401 tatcttcgat atgcatacag cgcaagttta catagatgtc tgttcgccct tccggatcga
     2461 gccatccgat aaaaccggag taatagcggc ggtcgtagcc ttcattctgc aaaataaact
     2521 gataggcttc ttccttcggc aagccgcata cggcaggtgt cggatgcaaa actttcagaa
     2581 ggtctcccaa acctttatta tctttcaaag caaaccggaa atcagttttt aaatgtgaca
     2641 aagcaccggc ataagcagga tatggtccgt tttccgtagc atggatatcc agtgaaagaa
     2701 gctgacgacg gatataagaa gcaacataat cttgctcttt ccggttcttt tcatcccata
     2761 cttgcggcag tctgccatct tgcaaaggct gtgttccggc tagcgcaacc gtatgccact
     2821 cgtctttctc acctgataaa ataatttcgg gagtgcttcc cagccagatg cccgtctgcg
     2881 gagtataaca taaatatata tacgaatgaa tataacgttt acaagctgcc cgaaagatgg
     2941 acaaaggaga aaaatccgct actttatcca ctgtaagatg acgggaaagc accagtttat
     3001 caaacgtcct gtcacgtaac gcatttataa aggcatgaaa gcatgccgca tattcttctg
     3061 tagaagaagt cagaaacgat tcttgtcctt gcaggcgcaa tgccacctcc cggtcttctt
     3121 ccgtatcatc atccaccagt aaaggctgcc cccactgatc gggttggatc aacacaaccg
     3181 gacatgactc actcacctga aacggagcga ttacaaatcc tctttgtccg ttcagttctt
     3241 tcagatcgta tatcaagcgg accgctcctt ccgcctgcgt caacagacgg ggaactttct
     3301 ctccgggaat acggtaaaca gcaaacggct gcttccgctg aataaatgca tcaatagttg
     3361 tcaagttact tatttcttcg tcggtcatct ctttttcatc acactgttga ctacccgtat
     3421 ggaagacacc agtttgtttg ttgaagtaaa cacgtctaca ttccatacat gagaggaacg
     3481 tcctttatgt acaacggttc ctactgcacg cacagtatct ccttcatgtg cagaagatat
     3541 atggtttccg ctgacttgca ttcctactac aatctcgtcc ggctggcaaa gaatcatcga
     3601 accgagcccg gcaactgttt ccgccaatgc aagagtagct cccccatgca aaatgccgaa
     3661 aggctgacgg gtacgatggt ctactggcat tgttgcttcc acacggtctt cgtcggcgta
     3721 tgtatattga attcccaagt ttcccattaa tgcgtggcgt gcctgcgcat tcagttggtc
     3781 aaggggaatt tcggctgcac gcacttcggc tataatagct ttctccaccg caacggccac
     3841 tccgtcttct tcattcgaag cggtcacata gtcggcacag gctttcaccg agtcttgtga
     3901 atgccccata gctacgccca atcccgccaa ttgaatcatt gttacgtcac acacaccatc
     3961 gcctacagca atcact
//
