LOCUS       c00287_gnlPro..         3599 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IMPIMKPL_287.
ACCESSION   c00287_gnlPro..
VERSION     c00287_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 03:15:37
            Original ID  :: gnl|Prokka|IMPIMKPL_287
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3599
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3599
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[44:3000]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      45..3000
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3599
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3599
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(45..770)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg287_1"
                     /sec_met_domain="Radical_SAM (E-value: 3.7e-32, bitscore:
                     99.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(772..3000)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg287_2"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKINETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTNPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 caaggctgtc tggaatcata tccggacagc cttgcttttc tgttttagtt ggttatcaca
       61 ttcgtgaaat actctttgaa cagtttttct gcccgttcca gttgttccgg agtattttcc
      121 ttcacttctt tcaatttgta atcccatccc atgacttcgt atttgtgaac gcccagacgg
      181 tggtaaggaa gtatttccac ccgttgtatc tgccggtatt tgccgagact ttcacccagc
      241 aggcggatgt cttcttccga atcgctgtaa ccgggaacca gtacataacg cagccagaag
      301 gggtgttcat gttcttcgag ccaggcagcg gtgcggagtg tctgttcgtt gcttcgtccc
      361 gtcagcgtac ggtggcgttc gggattgaat tgcttgatgt ccagcagcac gaggtcagtc
      421 aggctaaaga gctcttccac cttttcattc cagatgcctc cgttactgtc cagacagata
      481 tggatgccgt tcgccttcag ctccttgaaa agaggaatca gcgcttctgc ttgcagggta
      541 ggctcaccgc cggagaaagt aatacctcct ttcttcccga agaatgcctt ttggctgata
      601 gccatttgca gaatctcttc cggagtggtg ggagtgcctc cttttacatc aatcgtatcc
      661 ggattggcac aatacagaca acggaacgga catccttgca ggaaaacgac gagccggagt
      721 cccggcccgt cgaatgttcc catactttcg taggagtgta catttatcat attacattct
      781 ttcgtggaat gaacggctga tgacttccaa ctgatgctcg cggctcaact tcacgaagtt
      841 cacggcatag ccggaaacgc ggatggtgag ttgcggatac ttttcaggat gttccatggc
      901 atcttccagc atttcgcggt tcagtacgtt tacgttcaaa tggtgggcac ctttggtgaa
      961 gtagccgtcc atcatggtta ccaggttctc tacacgttct tccggtgtcg ggcccagtga
     1021 tttaggaaca atggagaacg tattgctgat gccgtcctgc gagtcgcggt agcgcaattt
     1081 ggctacggag ctcaaggaag cgatggcacc gttcttgtca cgtccgtgca ttgggttggc
     1141 acccggagca aaggcaacac ctttggcgcg tccgtccgga gtggcaccgg tcttcttgcc
     1201 gtacatcacg ttggaggtga tagtcagcaa ggaaagggtg ggacgggcat tcttgtagac
     1261 aggcagcttt ttcagttctt cgctgaaata gtaaacgagg tctactccca ggtggtccac
     1321 acggtcgtca ttattgccga agcaggggaa tgagccttca gtgtcgaaac cttcggtcag
     1381 gccgatttcg ttgcggcgtg cagtgacttt ggcatatttg atggcggaca gtgagtctat
     1441 ggcgatggaa aggccggcaa caccgtatgc cagattgatg cgtgggttgg tatcgatgaa
     1501 tgccatctgg gccttctcat aataatactt atcatgcatg tagtggatga tattcatggc
     1561 ctcgttgtag acacgggcta tttcagtcag taccttcttg tagttggcca taacttcctc
     1621 aaacttcagt gtctcgccgg tcaatacggg aatacctttc accatgacag ttccggtgtt
     1681 ttcgcaacgg ccaccgttga tggcaagcag cagggccttg gcaaggttgg cacgtgcgcc
     1741 gaagaactgg atttgcttgc cgatggcttg gtaggataca cagcaggcaa tgccgtaatc
     1801 gtcgcagttg cgcacttcgc gcatcaggtt gtcgttttcg tactggatgg agctggtatc
     1861 tacggaaact ttggcgcaga aatccttgaa gccttccggc agttcgggac tccacagtac
     1921 ggtcaagttt ggttccggag aaggaccgag gttgtatagc gtctgcaaga agcggaaaga
     1981 agtcttggtg actttcacgc gtccgtcatt gaagcgtcct ccgatggctt cggtcaccca
     2041 agtcgggtca ccggcaaaga tatcattgta cgactgcata cgcagatggc gtaccatgcg
     2101 cagcttgatg acgaactggt ctatcagttc ttgcgcaaac gtctcattga tttttccgtg
     2161 agccagatcg tattcaatga atatatcgag gaacgaagat acgttaccca atgacatggc
     2221 agcaccgtct tgctctttga tggcagcaag atatgccatg tatacccatt gcacggcctc
     2281 ttgagctgat gtagccggat ggctgaggtc gagtccgtaa tattcgccca taaccttgat
     2341 gtccttcaaa gctttgattt gttccgccac ttcttcgcgt agacggatgc gtgcttcggt
     2401 catcgggccg gtgaggttgc gcaagtcctc ctgcttggct tcaatcagac ggtcgatgcc
     2461 gtagagagcc aatcggcggt agtcgccgat gatacgtccg cgggcatagt tgtcgggcaa
     2521 tccggtaagg aatcccagtg agcggaagga acggatttct tcggtatata catcgaatac
     2581 gccgtcgtta tgtgttttgc ggtaatgggt gaagatgtct tttactttat cgtccacttc
     2641 cactccgttt tcacggcatg cttttgctac cacgttgatg cctccgaaag gtttgatgga
     2701 tcgtttcaac agttcatcag tctgcaaacc gacaataagt tcattgtcct tatcaatata
     2761 cccggcttta tgagaagtga tggtagatac ggtggtattg tccagtgaac ggacgccatt
     2821 gttgttcctt tcttcttcga gagccttcag acagatgtcc cagatacgtt tggtacgttc
     2881 ggtaggtccc tgcaagaaag atgcatcccc ggtatagggt gcgatgtttt tactgacgaa
     2941 atcactgaca ttgatttctt tattccaaag cccattggta aaggttttgt tcaattccat
     3001 aaaatttgtt tttgttagta ggttagataa tgttatcctt tagagcgcac aaaaatactg
     3061 aataaattcc gtaattttat aagagatact tctcttttta tgttgaacag cataaaataa
     3121 ctattattag gtagtgaatg tccgggggag tagagatgtt aatactttct atatgtggtt
     3181 tatgaattga aacaagaacc gaataataat ctgcattatt tacaaatgat gtaggtttaa
     3241 ggaacagtcg tattctgaac ctaattcagt cgtatagtga atgccattca gtcgtatact
     3301 gaatagggtt caatcgtata ctgaatgtgg ttctttgtaa atatactgat agccagtaag
     3361 ttataaaact ctgtcttgcc cttgataaat ttcaacaaat agcctaaatg ccgttatgtt
     3421 cgttccgtag tgggtataaa caagaatttt ggtataattg ctctgttttt gtgcagaaaa
     3481 gtttgtagaa taaaaaatga tttgtatctt tgcaccgctt taaaagaaaa gcacttctga
     3541 aaaagaagtt ttggagaggt ggcagagtgg tcgattgcgg cggtcttgaa aaccgttgt
//
