LOCUS       c00360_gnlPro..         2639 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IMPIMKPL_360.
ACCESSION   c00360_gnlPro..
VERSION     c00360_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 03:15:37
            Original ID  :: gnl|Prokka|IMPIMKPL_360
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2639
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2639
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[987:2637]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      988..2637
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2639
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2639
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..718
                     /locus_tag="ctg360_1"
                     /transl_table=11
                     /translation="YSIFQSYIAFIGKLFFSFGIIILFVACSVDYGQVEKDVRKELDNY
                     RERYVIAMYTGEVTLPSTNKYPHILHPAPGGMETGMNNVWLINGGYLGEIYECRGCDYC
                     MTRYHKAGLIDYQVFERNEDTVTAFVRLTPLGSRFLIENYVPPFYRLSQWRRREQIEIM
                     LVATEKFELEVVPSGKRTYHCNAHRSLALTPFLKAIGGTEREDSIDYVRQFKVEYDEKG
                     VGKVTRISSKVNDCGL"
     CDS             complement(988..1716)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg360_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.2e-30, bitscore:
                     94.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRQLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(1732..2637)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg360_3"
                     /sec_met_domain="Gly_radical (E-value: 3.6e-48, bitscore:
                     150.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 5.6e-89, bitscore:
                     286.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="TGTVMVKNIPVLTSDTLNFEEVMSNYKKVLTEIARVYNEAMNIIH
                     YMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTARRNDIGLTEGF
                     DIQGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVMYGKKT
                     GATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISNTFSIVPKSLG
                     ATEEDRVENLVTMMDGYFTKGAHHLNVNVLNREMLYDAMEHPEKYPQLTIRVSGYAVNF
                     MKLSREHQLEVISRSFHERM"
ORIGIN
        1 atattcaata ttccaatctt atattgcatt tatcggcaaa ctcttcttct cattcggaat
       61 cattatactc tttgtggctt gttctgtcga ttacgggcag gtggaaaaag acgtgagaaa
      121 ggaattggac aattacagag aacgctatgt catagccatg tacaccggcg aggtgacttt
      181 gccttctacc aacaaatatc cgcatatctt gcatcccgca cccggcggaa tggagaccgg
      241 aatgaataat gtgtggctca tcaatggagg atatctggga gaaatctacg aatgccgcgg
      301 ctgtgactat tgcatgacac gttatcacaa ggcgggactg atagattatc aggtcttcga
      361 acggaatgaa gacaccgtga cggcctttgt gagactgact ccgttgggaa gccggttttt
      421 aatagaaaat tacgtccctc ctttttatcg gctcagccaa tggcgcagaa gggaacagat
      481 tgagattatg cttgtcgcta ccgaaaagtt cgagcttgaa gtcgtcccat cggggaagag
      541 aacctatcat tgcaatgcac acagaagcct tgcgctgact ccctttttaa aagcaatcgg
      601 cggaacggaa agagaagata gcatcgacta cgtccggcag tttaaagtgg agtatgatga
      661 gaagggagtg gggaaggtga ctcgtatttc ttcaaaagta aacgattgcg ggctttaaat
      721 tctattgggt aaactcattc aggtatagca cacgaagttg tcaattaaat ccgttgtaca
      781 gtagtttcaa ggcttgacac aaaagtttct attttgcacc gcatacttgc tcttgagtta
      841 gtggaacttc tttctaatct ttttgtcgat tattcagagg aatacttttc tttgggagaa
      901 ccttcgctta aagtggctcg tgaaataagc aaaaagttga atatcaatgc tagtattgga
      961 tttttattct atttattact gtgatggtta attgaccacc acagtagtaa aatattcttt
     1021 aaatacctcc gcggcttttt caagttgttc gggggtattt tcttttacgt ctttcagctt
     1081 gtattcctgt tccatcgctt catacttatg tacgcctaat gtatgataag gcaggatttc
     1141 cactcgttgc accatcttgt actttcctaa tgcttctccc agttggcgga tgtcttcctc
     1201 gaaatcgctg tatccgggta ctaatacata acgcagccag aaaggtttct cgttttcttc
     1261 cagccatgct gccgtgcgga tggtttgttc gttacttctt ccggtcagtg cctgatggcg
     1321 tgccggattg aactctttaa tatccagtaa tacaagatcc gtcagtttga aaagttcttc
     1381 tacttcttcg ttccagatgc cgccgttgct gtctatgcat acatgtattc ccttttcttt
     1441 cagttcacgc actaaaggga ctagtgcttt ggcctgaaac gtaggttctc ctccggagaa
     1501 agtgactcct ccgcgtttcc cgaaaaaagg acgttggctc atagccatgc ggacgatttc
     1561 ttccggtggg gtaggtgtac ctccttttcc ggctatagtg tcgggattgg cgcaatatag
     1621 gcagcggaaa ttgcatcctt gaagaaaaac gacgagccgt aagcccggcc cgtcgaatgt
     1681 tcccatactt tcgtatgaat gtacgtttat cgtcatattg agtagatctg attacatacg
     1741 ttcgtggaag ctacggctga taacctccaa ttgatgttcg cggctcaact tcatgaagtt
     1801 tacagcataa ccggaaacac ggatggtgag ttgcggatat ttttccggat gttccatggc
     1861 atcgtaaagc atttcacggt tcagaacgtt cacgttcagg tggtgggcgc ctttggtgaa
     1921 gtaaccatcc atcattgtca ccagattttc tacgcgatct tcttcagtag ctcccagtga
     1981 tttcggaacg atagagaaag tgttgctgat accgtcctgt gagtcgcggt aacgcaattt
     2041 cgctacggaa ctcaaagagg cgatggcacc gttcttgtca cgtccgtgca ttgggttggc
     2101 tcccggagca aaagcaacac ctttggcgcg tccgtcggga gtagcacctg tcttcttgcc
     2161 atacattacg ttggaagtga tagtgagcaa agataaagta ggacgggcat ttttgtaaac
     2221 cggaagtttc ttcaattctt cgctgaagaa atagactagg tcaaccccca ggtggtctac
     2281 tttgtcgttg tcattaccga agcaagggaa ttcgccttga atgtcgaagc cttctgtcag
     2341 gccgatgtcg ttgcggcgtg cagtcacttt ggcatatttg atggccgaca gtgaatcgag
     2401 ggcgatagaa agtccggcta caccgtaagc aagattgatg cgtgggttgg tatctacaag
     2461 agccatttgg gctttttcgt agtaatactt atcatgcata taatggatga tgttcatcgc
     2521 ttcgttgtaa acacgggcga tttctgtcaa taccttcttg tagttgctca ttacttcttc
     2581 gaagttcagt gtgtcgctgg ttagtacggg gatgtttttc accattaccg taccggtgt
//
