LOCUS       c00253_gnlPro..         5453 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EOGLCEKM_253.
ACCESSION   c00253_gnlPro..
VERSION     c00253_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 06:39:00
            Original ID  :: gnl|Prokka|EOGLCEKM_253
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5453
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5453
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[846:4989]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      847..4989
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..5453
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5453
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             complement(115..840)
                     /locus_tag="ctg253_1"
                     /transl_table=11
                     /translation="MEKVGLFHFVAEPYLMDFRGRVTLPMIGNYLIHAASSHAGERGFG
                     FNDMSERHTAWVLSRLAIEMKEYPTAFDKINLYTWIDEVGRLFTSRCFELADENGKTFG
                     FARSIWAAIDVETRRPTLLDIEALGKYIDERPCPIEKPGKIMPAENKAEGIPYSIKYSD
                     LDINGHFNSVKYIEHLLDLFDIDQFKTREIGRLEIAYQSEGKQGMPLTLHKAESAPDKQ
                     DMAICHEGKAICRAAVTWR"
     CDS             complement(847..1401)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg253_2"
                     /sec_met_domain="ALS_ss_C (E-value: 4.9e-18, bitscore:
                     53.4, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTKKLYTVIIFSENIVGLLNQITIIFTRRQLNIETLSVSPSAIK
                     GIHKFTITTFADEDMIDKVVKQIDKRVDILKAYYNTDEDLVYQEIALYKLSTELFIKMG
                     TVEGLIRKYNARILEMNETCVVLEKSGHYDETQALFNELSETIGVLQFIRSGRVAITKS
                     KVERLSDMLADMERKLHEKQQ"
     CDS             complement(1417..3120)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg253_3"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.7e-51, bitscore:
                     161.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKQKITGSEALLKALIAEGVDTIFGYPGGQAIPIYDSLYDYRDQ
                     LRHVLVRHEQGATHAAQGYARVSGKVGVTLVTSGPGATNTITGIADALMDSTPMVVIAG
                     QVPSPLLGTDAFQEVDVIGITQPITKWAYQIRKPEEIAWAVSRAFYIASTGRPGPVVLD
                     LAKDAQVGLVEYEYKKVDYVRSYQPEPDINKDRIKAAADLINEAKKPFCLVGQGVLLGG
                     AEEELKAFLQKNDIPAGSTVLGLSALPTDFPLNKGMLGMHGNVGPNRKTNECDVLIAIG
                     MRFDDRVTGDLKTYAKQAKIIHLDIDNSEIGKNVAVDVKVLGNAKHTIPMITALLEERR
                     RLEWNAEFDRDEKEEYDKVIEKELYPTEGQLKMGEVVRKVSEATGNDAVLVTDVGQNQM
                     MGVRYFKYKQTRSVVTSGGLGTMGFGLPAAMGAKFGAPDRTVCFFTGDGGMQMTIQELG
                     TIMQEKLNVKIIILNNNFLGMVRQWQELFFHERYSNTIMENPDFVAIAKAYGIAGRAVE
                     KREELDDAIAEMLNHDGAYVLVANVETCGMVYPMVPAGGSVTNMIMGDEK"
     CDS             complement(3154..4989)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg253_4"
                     /sec_met_domain="ILVD_EDD (E-value: 1e-217, bitscore:
                     711.9, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNPLRSSFSTEGRRMAGARALWRANGMKEEQFGKPIIAIVNSFT
                     QFVPGHTHLHEVGQLVKAEIEKQGCFAAEFNTIAIDDGIAMGHDGMLYSLPSRDIIADS
                     VEYMVNAHKADAMVCISNCDKITPGMLMASMRLNIPTVFVSGGPMEAGELDGRHLDLID
                     AMIESADTSVSDERIEQVERHACPGCGCCSGMFTANSMNCLNEAIGLALPGNGTIVATH
                     KNRIQLFRDAAKQIVENAYKYYRDGDDSVLPRNIATRQAFLNAMSLDIAMGGSTNTVLH
                     LLAVAQEAGTDFHMEDIDMLSRKTPCLCKVAPNTHTYHVQDVNRAGGILGIMNELMKAG
                     LVDGSTRRADGLTLAEAVDKYAVTSPNVTEEAIRKYKSAPAHRFSIQMGSQESYYKELD
                     TDRAEGCIRDVEHAYSKDGGLAVLRGNIALDGCVVKTAGVDESIWKFSGPAKVFDSQDA
                     ACEGILGGKVVSGDVVVITYEGPKGGPGMQEMLYPTSYIKSRHLGKECALITDGRFSGG
                     TSGLSIGHISPEAASGGAIGLVRDGDIIEINIPERSINVRLSDEELAERRKAEEARGKK
                     AFTPPTRQREVSKALRAYGKMVSSADKGGVRIVED"
ORIGIN
        1 tttttttctg tctcatacaa tcaggaagga aagctctaca acaccgccaa aattcatttg
       61 tgccattttt ttttaactat taatgtttaa aaacctattt atgtatctct cttattatct
      121 ccaagtaacc gccgcacgac agatcgcctt tccctcatgg caaatagcca tgtcttgctt
      181 atcgggagcg gactccgcct tatgaagcgt aagcggcata ccttgtttac cctcggattg
      241 gtaggcgatc tccagacggc cgatctcacg ggtcttgaac tggtcgatat cgaacaaatc
      301 cagcagatgc tcgatatact tcacgctatt gaagtgaccg ttgatatcca agtcgctgta
      361 tttgatcgaa tacgggattc cctccgcctt attctccgcc ggcatgatct tgcccggctt
      421 ctcgatcggg cacggacgct cgtcgatata tttgcccaat gcctctatat ccaataaggt
      481 aggacggcgg gtctccacgt cgatagccgc ccaaatagaa cgggcgaatc caaaggtttt
      541 cccattctca tccgccagct cgaaacaacg actggtgaag aggcgtccga cctcatcgat
      601 ccaagtgtat agattaatct tatcgaaggc ggtaggatat tccttcatct cgatagccag
      661 acgggacaac acccaagccg tatgacgctc cgacatatca ttgaaaccga acccacgctc
      721 tccggcatgg ctggaagcgg cgtgaatcaa atagttacca atcataggga gcgttacacg
      781 tcccctgaaa tccatcaaat acggctccgc cacgaaatga aataatccta ctttctccat
      841 atcttattat tgttgctttt catgtaactt tcgctccata tcggccaaca tatcgctcaa
      901 gcgctccacc ttgctcttcg tgatagccac acgtccggag cggatgaatt gcaggacacc
      961 gatcgtctcg ctcaactcat tgaacaaggc ttgtgtctcg tcatagtggc cactcttctc
     1021 caataccacg caggtctcgt tcatctccaa gatacgagcg ttgtatttac ggatcaatcc
     1081 ctccacggtt cccatcttga tgaaaagctc ggtacttagc ttataaagag cgatctcttg
     1141 ataaaccaaa tcctcatccg tattataata agccttcaag atatccacac gtttatcgat
     1201 ctgtttcacc accttatcga tcatatcctc atcggcgaat gtcgtgatcg taaacttatg
     1261 gatacccttg atagcggacg gagagacgga cagagtctcg atgttcagct gtcggcgtgt
     1321 aaagataatc gttatctggt tcaaaagacc tacgatattc tctgaaaaga taataacggt
     1381 atataatttc tttgttgtca tagcttcatc tcctccttat ttctcgtctc ccataatcat
     1441 gtttgtcacg cttcccccgg caggaaccat cggatatacc attccgcaag tctccacgtt
     1501 agcgaccaac acgtaagcgc cgtcgtggtt tagcatctca gcgatagcgt cgtccaattc
     1561 ctcccgcttt tcaacggcac ggccggcgat accataggct ttagcgatcg ccacgaaatc
     1621 cggattctcc atgatggtgt ttgaataacg ctcgtggaag aacaattctt gccactgacg
     1681 taccatacct aagaagttat tattaagaat gataatcttc acgttcagtt tctcctgcat
     1741 gatagtgccc aattcctgaa tcgtcatctg cataccccca tcaccggtaa agaagcagac
     1801 cgtacggtcc ggagctccga acttagcgcc catagcggcc ggcagaccaa atcccatcgt
     1861 acctaaacca ccggaagtca ccacgctacg ggtttgttta tatttgaaat agcggacgcc
     1921 catcatctgg ttctgcccaa cgtcggttac caatacagcg tcgttaccgg tcgcctcgga
     1981 aaccttacgg actacctcgc ccattttcag ttggccctcg gtcggataca attccttctc
     2041 gattacctta tcgtattcct ctttctcgtc ccgatcgaac tcggcattcc attccagacg
     2101 tctacgttct tccaacaacg ccgtgatcat cggtatcgtg tgcttggcat tacccagcac
     2161 ctttacatcc acggcaacat tcttgcctat ttccgagtta tcgatatcca gatggataat
     2221 cttggcttgc ttagcgtatg tctttaaatc gccagtcaca cggtcgtcga aacgcatacc
     2281 gatagcgatc aatacatcac actcattcgt tttccggttc ggccctacgt ttccatgcat
     2341 gccgagcatt cccttgttca atgggaaatc ggttggcaac gcagataagc ccaataccgt
     2401 agatccggca ggtatatcat tcttttggag aaacgctttc aattcctctt ccgcaccgcc
     2461 taacaatact ccctgtccga ccagacagaa gggtttcttc gcctcattga ttaaatcggc
     2521 ggcggccttg atacgatcct tattgatatc cggctccggt tgataactac gtacgtaatc
     2581 gaccttctta tattcatatt ccaccaaccc tacttgcgcg tccttcgcta aatccagcac
     2641 aactggaccc ggacggcctg tagaagcgat ataaaaagca cgagaaacag cccatgcgat
     2701 ctcctccggc ttacggattt gataagccca tttcgtgatc ggttgcgtga tgccaatcac
     2761 gtcgacttcc tgaaaagcat cggttcccaa caacggcgag ggtacttgac cggcgattac
     2821 gaccattggc gtactatcca tcagcgcatc agcgataccc gtgatggtat tcgtagcgcc
     2881 cgggccggaa gtaaccaacg tcactcctac cttgccggat acacgggcat atccttgagc
     2941 cgcatgggta gcgccttgct cgtggcgcac caatacatgc ctcaactggt ccctataatc
     3001 atataaactg tcgtaaatag gtatcgcctg tcctccggga taaccgaaga tagtatctac
     3061 tccttcagca atcaacgctt tcagcaaagc ttccgaaccg gttatctttt gtttctccat
     3121 aagaaataac gtgtttaatg tatattattg gctttaatct tcaacaatcc ttacacctcc
     3181 cttatcggcg gagctaacca tcttcccgta cgctctcaac gctttcgaga cctcacgctg
     3241 acgcgtcggc ggcgtaaagg ctttctttcc acgtgcctct tccgctttac ggcgctcggc
     3301 caactcctcg tcgctcaacc gcacgttgat ggaacgttcc gggatattga tctcgatgat
     3361 atccccgtct ctcaccagtc cgatagcgcc accggaagcg gcctcgggag agatatgccc
     3421 gatagaaagg ccggacgtac cgccactgaa acgaccatcc gtgatcaagg cgcactcctt
     3481 acctaagtga cggctcttta tataagaagt aggatataac atctcttgca ttcccggccc
     3541 ccctttcgga ccctcatacg tgatgacgac cacatcgcct gaaacgacct tgcctcctaa
     3601 aatgccctcg caagcggcat cctgtgaatc gaagactttc gccgggccgg agaatttcca
     3661 aatactctca tccacaccgg cggtttttac gacacatccg tccaaggcga tattccctct
     3721 caaaacagcc aagccgccat ccttggaata agcgtgctcc acgtcgcgga tacaaccttc
     3781 ggcacgatcc gtatctaatt ccttataata agattcttgg gaacccatct ggatactgaa
     3841 ccgatgagcg ggagcgctct tgtatttacg gatcgcctcc tccgttacgt tcggggaagt
     3901 gacggcgtat ttatcaacag cctcggccaa ggtcaggccg tccgcacgtc tcgtgctgcc
     3961 atccacaagc ccggctttca tcaactcgtt catgataccg aggattccac cggcacggtt
     4021 tacgtcttgt acatgataag tgtgcgtatt cggggctacc ttacacaggc aaggagtctt
     4081 gcgggataac atatcgatat cctccatatg aaaatcggtg ccggcttctt gtgccacggc
     4141 caataagtga agcaccgtat tggtagaccc ccccatggcg atatccagcg acatggcgtt
     4201 caagaaagcc tgacgggtag cgatgttgcg aggtaacacg gaatcatccc cgtcacgata
     4261 gtatttgtaa gcgttctcaa cgatttgctt agccgcgtca cggaaaagtt gaatacggtt
     4321 cttatgggta gccacgatcg tcccgttacc cggaagcgcc agaccgatcg cctcgttcaa
     4381 gcagttcata gagttagcgg taaacatacc ggaacaacaa ccgcaacccg gacacgcatg
     4441 gcgttccacc tgctcgatac gctcatcgct taccgatgta tcggcagact caatcatcgc
     4501 gtcgatcaaa tcaagatggc ggccatccag ctcacctgcc tccatcgggc caccggacac
     4561 gaacaccgtc gggatattta atcgcatgga agccataagc atacccggcg tgatcttatc
     4621 gcaattgctg atgcaaacca tagcgtccgc cttatgggca tttaccatat attccacgct
     4681 gtccgcgata atgtcgcgtg aaggaagcga gtaaagcatc ccatcgtgcc ccatagcgat
     4741 accgtcgtcg atagcgatcg tattaaattc agcggcgaaa caaccttgtt tctcgatctc
     4801 ggccttgacc aattgcccga cctcatgcaa atgcgtatgc cccggcacga actgggtaaa
     4861 tgaatttacg atagcgataa taggcttgcc gaactgttcc tctttcatcc cgttggcacg
     4921 ccatagcgca cgggctccgg ccatcctacg accttcggtg ctaaatgagc ttcttaacgg
     4981 attcttcatc tctttcctat tttttgttcg tagagcgctt tatccacgcc tctaccttga
     5041 tttatttata ataaaaaagc ctttccctat atggagaaag gcttaagaaa tatctctacg
     5101 acttcacaac ttcttacaca cctttctcct tacctactaa tgactagaag gaccaccacg
     5161 agaataatat gcaatacgtt gttcatcatc ttttcttatt gtctttgttt gtttgacgct
     5221 gcaaatgtag aacaataatt gatatgcgca aaggatttaa aagaaaattc ttcatttttc
     5281 tttcaattct caagcaaata ccattttagg attgcatttt aacaggagca agagtgtatt
     5341 acttaggaat cggaaggtat agaagggaaa aaacttcgat ttccaaacag aaacagtaaa
     5401 aagacaaatg ttatctaaaa cagattccag ataacatttt tatcagataa cac
//
