LOCUS       c00333_gnlPro..         4563 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EOGLCEKM_333.
ACCESSION   c00333_gnlPro..
VERSION     c00333_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 06:39:00
            Original ID  :: gnl|Prokka|EOGLCEKM_333
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4563
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4563
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[38:4562]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      39..4562
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..4563
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4563
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             39..449
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg333_1"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.1e-27, bitscore:
                     85.0, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELTHIEHLGIAVKSIEACLPYYEGVLGLKCYNIEVVADQKVKTA
                     FFKVGQTKIELLEPTSEDSTIAKFIEKKGEGIHHIAFAVPDVQAALNEAEEKGVKLIDK
                     APRGGAEGLNIAFLHPKSTCSVLTELCMPGNK"
     CDS             486..2039
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg333_2"
                     /sec_met_domain="CT_Btheta (E-value: 4.2e-293, bitscore:
                     960.5, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKVKELIALREQARLGGGEKRIESQHKKGKYTARERIAML
                     LDEGSFEEFDMFVQHRCTNFGIEKTKYLGDGIVTGYGTIDGRLVYIFAQDFTVFGGALS
                     EALAMKICKVMDQAMKMGAPVIGLNDSGGARIQEGVNALAGYAEIFQRNILASGVVPQI
                     SGIFGPCAGGAVYSPALTDFNIMTRGTSYMFLTGPKVVKTVTGEDVTQEQLGGASVHTT
                     KSGVAHFAVDTEEDGLQLIRKLLSFLPQNNLEETPLVECKDPIDRLDDVLNDIIPDNAN
                     QSYDMYEVIGTIVDNNEFLEVHADYAKNIIVGFARFNGQSVGIVANQPKVMAGCLDSNA
                     SRKAGRFVRFCDAFNIPLVTLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKITV
                     TLRKSYGGAYCVMSSKHLRGDMNYAWPTAEIAVMGPSGAVEVIFAKEVAAAEDPKACAA
                     EKEAEYKKAFANPYNAAQYGYIDDVIEPRNTRFRIIRALQQLQTKKLSNPAKKHDNLPL
                     "
     CDS             2061..2954
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg333_3"
                     /sec_met_domain="OAD_gamma (E-value: 6.7e-14, bitscore:
                     40.9, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINKKIGVLLLLMVLFNMGAKAQLTTSVKLNEVLVVNEDNFVDDY
                     GKRHPWFELFNNSAGTVDLRGCFLTNDKSNPRKYMIPKGDVLTKVPPRQHLLFWADDQP
                     TKGTFHVNFTFDPNKDNYLALYDSDGTTLIDEVTIPAGQKADVSYGLDLDGVGNWSILD
                     KVTPSTNNLTLDSNAKLENFQRNDPWGIGMTITAMAVVFLGLIILYLVFKQIGNAAIKA
                     SKRNAQKAAGTGAAVSANIGQESGEIFAAIATALYEMSDDNHDIENTVLTIHKVQRSYS
                     PWSSKLYSLRQTPNKK"
     CDS             2978..3409
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 63.7; E-value:
                     3.5e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg333_4"
                     /sec_met_domain="Biotin_lipoyl (E-value: 2.9e-21, bitscore:
                     63.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSFKYTINGNTYKVHINSVVDDVAEVEVNGTPYNVKMEKPAKKQ
                     MVTLKRPAQAPTNASGEPVITRQAAPSVAGAVKSPLPGVILSVDCKVGDTVKRGQKILV
                     LEAMKMENNINADRDGKIVEIKVNKGDSVLEGADLVVIG"
     CDS             3414..4562
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg333_5"
                     /sec_met_domain="OAD_beta (E-value: 5.6e-154, bitscore:
                     500.6, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQESFLQFLGENIQIFLSYTGFANATPGHIVMLLVGLLFIFLAIK
                     YEFEPMLLIPIGFGILIGNIPFKDAGLQLGIYEEGSVLNILYQGVKQGWYPPLIFLGIG
                     AMTDFSALISNPKLMLVGAAAQFGIFGAYIIALQMGFDPSQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPIIQPPFMRLLTTKKERVIRMKPPRAVSQTEKIVFP
                     IVGLLLTAFLVPSGLPLLGMLFFGNLIKESGVTRRLAETARGPLIDVITILLGITVGAS
                     TQATQFLTLNSIKIFGLGALSFVIATCAGVLFVKFFNLFLKEGNKINPLIGNSGVSAVP
                     DAARISQNVGLEYDPSNYLLMHAMGPNVAGVIGSAVAAGILLG"
ORIGIN
        1 gaaacaaaca tttactaaat ctttaaatta ttattgacat ggaacttaca catatcgaac
       61 atttaggtat cgcagtgaag agtatcgagg cttgtttacc ctattatgaa ggcgttcttg
      121 gattaaaatg ctacaatatc gaagtagtag cagaccaaaa agtaaaaacc gcatttttca
      181 aggtaggaca gactaagatt gaattactag agccgaccag cgaagatagc acgatcgcaa
      241 aatttatcga gaaaaaaggt gaaggtatcc accatatcgc attcgcggta cctgatgtac
      301 aggcagcttt gaacgaggct gaggaaaaag gcgttaaatt aatcgataaa gctccccgtg
      361 gtggcgcgga aggcttgaac atcgctttcc ttcacccgaa atcaacttgc agcgtattga
      421 cagagctttg catgccgggc aacaaataat taatccgttc atttctaaca acaaatcaaa
      481 atacaatgag taaccagctt gaaaaagtaa aagagctcat cgcacttcgt gagcaagctc
      541 gcttgggtgg aggagagaaa agaatcgaat cacagcacaa aaaaggcaaa tataccgctc
      601 gtgagcgtat cgccatgttg cttgacgaag gtagtttcga ggaattcgac atgtttgttc
      661 aacaccgttg tacaaacttc ggtatcgaga aaactaaata tttaggcgat ggtatcgtaa
      721 caggttatgg tacgatcgac ggacgtttgg tatatatctt cgctcaagat ttcaccgtat
      781 tcggtggagc cttgtctgag gctttggcta tgaagatctg taaagtaatg gatcaagcca
      841 tgaagatggg cgctcccgtt atcggtttga acgactccgg tggagctcgt atccaagaag
      901 gtgtaaacgc cttggcagga tacgcagaga ttttccaacg taacatcttg gcttccggtg
      961 ttgttcccca gatttccggt atcttcggcc cttgcgccgg tggagccgtt tattctccgg
     1021 ctttgacaga cttcaacatc atgacacgtg gtaccagcta tatgttcttg actggtccga
     1081 aagtcgtaaa aaccgtaaca ggtgaggatg taacccaaga gcagttgggt ggagccagcg
     1141 tacacactac caaatcgggt gtagcccatt tcgctgtcga tacagaagaa gatggtttgc
     1201 aattgatccg taaattgctc agtttcttac ctcagaacaa cttagaggaa acgccgttag
     1261 ttgagtgcaa agacccgatc gatcgtctgg atgatgtatt gaacgatatc attcccgata
     1321 acgccaacca atcttacgat atgtatgagg ttatcggtac gatcgtcgat aacaacgagt
     1381 tcctagaggt ccatgcggac tacgctaaga atatcatcgt aggtttcgct cgtttcaacg
     1441 gacaatccgt cggtatcgta gctaaccagc cgaaggttat ggcaggatgc ttggatagca
     1501 acgcatctcg taaagcaggt cgcttcgttc gtttctgcga cgctttcaat attcctttgg
     1561 taacattggt tgacgttccg ggattccttc cgggtacagg tcaggaatac aacggtgtaa
     1621 tccttcatgg cgctaaattg ctttacgctt atggtgaggc taccgttcct aagattaccg
     1681 ttacattgcg taagtcttac ggtggagctt attgcgtgat gagttctaaa catttacgtg
     1741 gtgatatgaa ttacgcatgg ccgacagccg agatcgccgt tatgggcccg agcggtgccg
     1801 tagaggttat cttcgccaag gaagtagcag ccgctgaaga tccgaaagca tgcgcagcag
     1861 agaaagaagc tgaatataag aaagcattcg ctaatcctta taacgcagct caatacggtt
     1921 atatcgacga tgttatcgaa ccgcgtaaca ctcgtttccg tattattcgt gctttgcagc
     1981 aattgcagac taagaagtta agcaatcctg ctaagaaaca tgataacctg cctctataat
     2041 ccgtaaaaca ggagatgaat atgataaata agaaaatagg ggtattgcta ctgttaatgg
     2101 ttctcttcaa tatgggagcc aaggcacagt tgactacctc cgttaaatta aatgaagtgc
     2161 tagttgtcaa tgaagataac tttgttgatg actatggaaa acgccatcca tggtttgagt
     2221 tatttaataa ctcagcaggt acggtggatt tacgaggatg cttcttaacg aacgataaaa
     2281 gtaatcccag aaaatatatg atcccgaagg gagatgtact taccaaagtt cctccccgcc
     2341 agcatcttct tttttgggca gatgatcagc caacaaaagg gacatttcat gtaaacttca
     2401 cattcgatcc aaacaaagat aattatctag ccttatatga ttcagatggt acaactttga
     2461 tcgacgaggt gacaatccct gccgggcaaa aagcagatgt tagttacggt ctggatcttg
     2521 atggagtagg aaattggagt atccttgaca aagtgactcc tagcaccaat aatctgacgt
     2581 tagattcgaa tgcgaaactt gagaacttcc aacggaacga cccatggggt attggtatga
     2641 ccataacagc catggcagtc gtattccttg gcttaatcat tttgtatcta gtattcaagc
     2701 agatcggtaa cgcagcgatc aaagctagca agcgtaacgc tcaaaaagcc gcaggaacag
     2761 gtgctgccgt atcagccaat atcggtcaag aatcaggtga aatttttgcc gctatcgcta
     2821 ccgctcttta tgagatgagc gatgacaacc acgatatcga gaacacggta ttgactatcc
     2881 acaaggttca acgcagctat tcaccatgga gttctaagct ttattctctg cgtcagactc
     2941 caaataaaaa gtaatcatcc taaaatccaa ttgacgaatg aaaagtttta aatataccat
     3001 taacggtaac acatataaag tacatatcaa ctcagttgta gatgatgtgg cagaggtaga
     3061 ggtaaacgga accccttata acgtaaaaat ggagaaacct gcgaaaaaac aaatggttac
     3121 attgaaacgt ccggctcaag ctccgaccaa tgccagcggt gaacctgtta tcacccgtca
     3181 agcagctcca agtgtagccg gcgctgttaa atctccgctg ccaggtgtaa ttttaagtgt
     3241 agactgtaaa gtcggcgata cagttaagag aggccaaaag atcttggtat tggaagccat
     3301 gaagatggaa aacaacatca atgccgatcg tgacggtaaa attgtagaaa ttaaagtaaa
     3361 caaaggcgac tctgtattgg aaggtgcaga tctcgttgta atcggttaat gatatgcaag
     3421 aaagtttctt acaattccta ggagaaaata tccaaatatt tctctcctat acaggatttg
     3481 cgaacgcgac tccagggcat atcgtgatgc tattggtagg tttgctgttc atattcctag
     3541 cgattaaata tgaattcgag ccaatgcttc tgatccctat tggtttcggt atcttgatcg
     3601 gaaatatccc tttcaaggat gccggattac aattaggtat atacgaagag ggttctgtat
     3661 tgaacatttt ataccaagga gtgaaacaag gttggtatcc ccccttgata ttcctcggta
     3721 tcggtgccat gacagacttc tccgctttga tatccaatcc taagttaatg ctagtcggag
     3781 ctgccgccca atttggtatt ttcggtgctt atatcattgc attgcaaatg ggatttgacc
     3841 cgagccaagc cggtgctatc ggtattatcg gaggagctga tggtccaacc gcaatcttct
     3901 tgtcatccaa gttagctcct aacttgatgg gtgctatcgc tgtatccgca tactcatata
     3961 tggcattggt tcctattatc cagccaccgt ttatgagatt gcttacaacg aagaaagaac
     4021 gtgtgatacg tatgaaacct ccaagagccg tttcacagac ggagaaaatc gttttcccga
     4081 tcgttggctt gctattgaca gccttcttgg taccgtctgg tttgccattg ttaggaatgt
     4141 tgttcttcgg taacttgatc aaggaaagtg gtgttactcg ccgtttggca gagacagccc
     4201 gcggcccgct gatcgatgtt attacgatct tgttaggcat cacggtaggt gcttctacac
     4261 aagctaccca gttcttaaca ttgaactcta tcaagatttt cggtttgggt gcgttgtcat
     4321 tcgttatcgc tacttgtgcc ggtgtattgt tcgttaagtt ctttaacctg ttcttgaaag
     4381 aaggcaacaa gatcaatccg ttgatcggta actccggtgt atccgccgta cctgacgctg
     4441 cccgtatttc ccagaacgta ggtttggaat acgatccaag caactacttg ttgatgcacg
     4501 ctatgggtcc aaacgtagca ggtgtaatcg gttccgctgt agctgccggt atcttgctag
     4561 gat
//
