LOCUS       c00399_gnlPro..         3822 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EOGLCEKM_399.
ACCESSION   c00399_gnlPro..
VERSION     c00399_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 06:39:00
            Original ID  :: gnl|Prokka|EOGLCEKM_399
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3822
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3822
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[714:3690]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      715..3690
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3822
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3822
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..544
                     /locus_tag="ctg399_1"
                     /transl_table=11
                     /translation="INIIQSNNELLLRLINDILDLSKIESGIIERRKENFNLAKVCNEL
                     FVTIQAKMTNPNVELRLDGPNSECWVLLDRNRLKQVWMNFLTNAVKCTKSGYIKMGYGI
                     ERKGLRIYVEDTGIGIPDDLHDKVFTRFEKLNEFSQGTGLGLTISRAIVEAAGGEVGFT
                     SKLGVGSTFWAWLPLRP"
     CDS             715..2955
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg399_2"
                     /sec_met_domain="Gly_radical (E-value: 1.2e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 9.2e-283, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             2956..3690
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 68.1;
                     E-value: 3.3e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg399_3"
                     /sec_met_domain="Radical_SAM (E-value: 5.3e-34, bitscore:
                     105.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLQYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
ORIGIN
        1 tataaatatc atccaatcca ataatgagtt gttattgcga ttgatcaacg atatccttga
       61 tctttccaag atagagtcag gcataattga acgtaggaag gagaatttca atctggctaa
      121 ggtttgtaat gagttatttg ttacgattca ggcgaagatg acaaatccga atgtcgagtt
      181 acgtttagat ggtcccaact cggagtgctg ggtacttctg gatagaaacc ggcttaagca
      241 agtatggatg aatttcctca ctaatgcggt aaaatgcacc aaatccggat atattaaaat
      301 gggatatggc attgaaagga aaggtcttcg tatctatgtt gaggatacgg gaatcggtat
      361 accggatgat ttgcatgaca aagtatttac ccgttttgag aaattgaatg agttctcgca
      421 gggtaccgga cttggcttga cgatctccag agctatcgta gaagcggcag ggggagaagt
      481 gggctttact tcaaaacttg gtgtcggatc tactttttgg gcatggttgc ctcttcgccc
      541 ttaggtctga ctccccattt attggtaatg tccttttaaa atagtgaagg atgagaagct
      601 tttttatcgc aatttatagg gattttagaa ctggtgtagc atatgtgaca gaaatatgac
      661 gataattctc ctacctttgc ggcggtaaaa atgtggtatt agataagatt gtttatgaaa
      721 tttgagaatg agtgctggga tcactttaag ggtgagacct ggaaaagaga gatcaatgta
      781 agagatttta tccagagtaa ctatacgcct tatgaagggg atgattcctt tttagtcgct
      841 tcttccgaga agacccgtaa ggtctggaac aagctgaccg agatgtttaa ggtcgagagg
      901 gaaaagggag tttatgacgc cgagacaaaa ttacctcaag ggatcgatgt atatggcccg
      961 ggctatattg ataaggagaa tgaggtgatc gttgggttac aaaccgatgc tccgctgaaa
     1021 cgtggtatct tcccgaaggg cggtatccgt atggttgaga atagcttgga agcttatggt
     1081 tatcatttgg acccgatgac gaaagagatc ttcacgaagt accgtaagac gcataacgag
     1141 ggcgttttct ccgcatatac ggaagagatg attgccgcac gtcgttcggc tattatcacc
     1201 ggtctgcccg acgcatatgg tagaggacgt attattggtg actatcgtcg tgtcgctctg
     1261 tatggtacgg caatcttgat cgaggagaaa aagcgtttct tgaatcgttt ggatatccaa
     1321 gagatcacgg aagagatcat ccagagccgt gaggagattt ccgaacagat caaggctttg
     1381 aaagcgttcg agaaaatgtg cgctagctat ggctttgatg tgactcgtcc ggcacaaaac
     1441 gctcgtgagg cagttcagtt tgtgtactta gcttatctgg cggcggtaaa agaccaagat
     1501 ggcgcggcga tgtctatcgg acgtacttct acgtttttgg atatttatat cgagaaagac
     1561 atccgtgagg gtaagttgac cgaagaggag gcgcaagaat tggtagacca gctgatcatc
     1621 aagttgcgta tcgttcgttt cttacgtacg ccggaataca atgatttgtt ctccggtgat
     1681 ccggtttggg taacagagtc cttgggcggc caaggagtgg atggccgttc tttggtaaca
     1741 agaacttcct atcgttatct ccatacatta tataatctag gtccggcacc agaacccaat
     1801 ttgacggttc tgtggtttaa gaacgctccc gagaactgga aacgtttctg tgccaaggtc
     1861 tctatcgata cgtcggctat ccaatatgag aacgacgatt tgatgcgtcc ggattatgga
     1921 gatgattatg gtatcgcttg ttgcgtttct cctatgaaga tcggtaagca gatgcagttc
     1981 ttcggagccc gtgcgaattt ggcgaaatgt ttattatatg cgatcaacgg aggacgggat
     2041 gagcgcagcg gcgtacaggt agccccgatg ttcgagccgg ttcgcggcga gtatctggaa
     2101 tacgatgagg tgatggctaa gtatgagcaa atgatgcgct ggttggcgaa agtgtatgtg
     2161 aacgccttga agataatcca ttatatgcat gataaatacg cgtatgaggc attcgagatg
     2221 tcgcttcacg atggggatgt agagcgtatc cgtgcgacag gtatcgccgg tctttctatc
     2281 gttgccgatt cgttggctgc tatccgtgat acgaaagtac gtgtgatccg tgacgagcgt
     2341 ggcttggcgg ttgatttcga acgtgagggc gaatatgttc ctttcggtaa taacgatgat
     2401 cgtacggata gtatcgctgt agagatcacc gagaagttca tggaatattt gcgtcagcac
     2461 gagacctatc gccatgcgaa acctacgcag tccatcctta cgatcacctc taatgtggta
     2521 tatggtaaga agactggtac gacaccggat ggccgtatcg gtggaactcc tttcgctccg
     2581 ggcgcgaacc ctatgaacgg tcgtgatacg aaaggtgcta tcgctgcgct agcttcggta
     2641 gcgaagcttc cattccagca tgcgcacgac ggtatctctt atactttcgc tgtttctccg
     2701 gctacgttgg gaaaggagcg tgaagtgcag atcaataatc tggttagctt attggatggc
     2761 tattttactc cggatggagg tcagcatctg aatgttaacg tatttgataa ggatttgttg
     2821 atcgacgcta tggaacatcc tgagaaatat ccgcaactta cgatccgtgt atccggatat
     2881 gcggtaaact tcgtgaaact gactcgtgaa caacagttgg atgttatttc aagaaccatt
     2941 aaccattcac tgtaagtgat aaaaggtaag atacattcat tagagagttt cgggaccgta
     3001 gatggtcccg gaattcgctt tgtcgttttt atgcagggct gcccgttgcg ttgcctgtat
     3061 tgccataacc cggatacttg gaacccgaaa gggaaggtga aatatcagat gactcccggc
     3121 gagctattga cggaagtctt acagtataag agttttatcg cccgtggggg ggtgactgtt
     3181 accggtgggg aacctttatt gcaacctgag tttctgaaag agtttttccg tttgtgccaa
     3241 gagcaaggtc ttcacacagc tttggacaca tctggtttcg tatgtacctc aaaagcttgg
     3301 gaagtcttgg attatgcgga cttagtgtta ttggacatca aaaccttaaa cccggattta
     3361 catccgttac tggcgggtgt caagcaggat aatacgcttc tgttcttgga tgaattggag
     3421 agacgtggca tagatacttg gattcgccat gttattgtcc ccggatatac ggataatgac
     3481 gagtggctgg aggcgttagc tcggtatgtc agttcatata aagtggttcg caaagtggaa
     3541 ttattgcctt atcatacgat gggaacctat aaatatgagc aattaggcct cgattatccg
     3601 ttaaaggggg tggaaccctt atcaaaagaa cgtttagata atgcgaaagc gatattctct
     3661 cgctacaaac cagaaaataa caaggcctga tctctcgatc aagccttgcg attatgcaac
     3721 agctaatcag ggaaaacaat ctaatccgat tagcttttaa acgatagtaa taattttttg
     3781 cctagactaa ccttgactat ttaatatgtg tttgttttgt ta
//
