LOCUS       c00157_gnlPro..         9124 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MDGKNFLA_157.
ACCESSION   c00157_gnlPro..
VERSION     c00157_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 03:55:47
            Original ID  :: gnl|Prokka|MDGKNFLA_157
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 9124
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..9124
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[596:5453]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      597..5453
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..9124
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /kind="single"
                     /product="TPP_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..9124
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_fatty_acids"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /tool="gutsmash"
     CDS             150..512
                     /locus_tag="ctg157_1"
                     /transl_table=11
                     /translation="MVQVRIKRVYEDFSETDGYRVLVDKLWPRGIKKEWLKYDYWAKDI
                     TPSAALRRWFHEDIPGHWNDFVVMYQKELEASQAVADFLTLIKPHPVITLLYASKEPVY
                     NHARILRDYLEMHLKE"
     CDS             597..1829
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: HBT"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg157_2"
                     /sec_met_domain="HBT (E-value: 1.6e-17, bitscore: 53.0,
                     seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYKLLVLDVDGTLLNDAKEISKRTLAALLKVQQMGVRIVLASGR
                     PTYGLMPLAKSLELGNYGGYILSYNGCQIINAQNGEILFERRINPEMLPYLEKKARKNN
                     FALFTYHDDTIITDTPENEHIQNEARLNNLKVIKEEEFSVAIDFAPCKCMLVSDDEEAL
                     VSLEGHWKRRLNGALDVFRSEPYFLEVVPCAIDKANTLGALLEELDVKREEVIAIGDGV
                     CDVTMIQLAGLGVAMGHSQDSVKVCADYVTASNEEDGVALAVEKAIIAEVRAAEIPLDQ
                     LNAQARHALMGNLGIQYTYADEDRVEATMPVDHRTRQPFGILHGGATLALGETVAGLGS
                     MILCQPDEIVVGMQVSGNHISSAHEGDTVRAVATIVHKGRSSHVWNVDVFTSTNKLVSS
                     IRVVNSVMKKR"
     CDS             1826..2938
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Chorismate_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg157_3"
                     /sec_met_domain="Chorismate_bind (E-value: 2.4e-58,
                     bitscore: 186.9, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDEKSNLTTIDALIQRKQPFAVYRVPGEKYPRLLTEDVGAVRLI
                     FDLKELNGQRGFVIAPFRIDKSCPIVLIQSDRTGQPLPMEIVAEEEQDLQSYPEESFHT
                     LCTGKYATCFHTFIEALRDATFDKLVLSRSLTIGKNPEFSPSAVFRAACQRYIHSYIYL
                     CYTPQTGVWLGSTPEIILSGEKNEWNTVALAGTQPLQNGKLPQVWDDKNRQEQDYVASY
                     IRRQLLSLGIRSTESGPYPAYAGALSHLKTDFHFSLKDNKNLGDLLKVLHPTPAVCGLP
                     KEEAYRFILENEGYDRKYYSGFIGWLDPEGRTDLYVNLRCMHIEDEQLTLYAGGGLLAS
                     SELNDEWQETEKKLQTMRRILVSAPIMMNH"
     CDS             2958..4625
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg157_4"
                     /sec_met_domain="TPP_enzyme_C (E-value: 4.4e-09, bitscore:
                     25.6, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYSDKKNILQLVALLEAHGITKVVLCPGSRNAPIVHTLSTHPGFT
                     CYAMTDERSAGYFAIGLALNGGHPAAVCCTSGTALLNLHPAVAEAYYQNIPLVVISADR
                     PAAWIGQMAGQTLPQPGVFQTLVKKSVNLPEIQTEEDEWYCNRLVNEALLETNHHGKGP
                     VHINIPISEPLFQFTVESLPEVRVITRYQGLNVYDRDYNDLIERLNRYQKRMIIVGQMN
                     LIYLFEKRHTKLLYKHFVWLTEHIGNQTVPGIPVKNFDAALYAMPEEKTGQMTPELLIT
                     YGGHVVSKRLKKYLRQHPPKEHWHVSADGEVVDLYGSLTTVIEMDPFEFLEKIAPLLDN
                     RVPEYPRVWENYCKTIPEPEFGYSEMSAIGALIKALPESCALHLANSSVIRYAQLYQVP
                     STIEVCCNRGTSGIEGSLSTAVGYAAGSDKLNFIVIGDLSFFYDMNALWNINVRPNLRI
                     LLLNNGGGEIFHTLPGLDMSGTSHKYITAVHKTSAKGWAEERGFLYQRVENEEQLAEAM
                     KTFTQPEAMEQPVLMEVFSNKNKDARILKDYYHQLKQK"
     CDS             4629..5453
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: ECH_1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg157_5"
                     /sec_met_domain="ECH_1 (E-value: 1.6e-82, bitscore: 265.8,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTQREWTTIREYEDILFDYYNGIARITINRERYRNAFTPTTTAE
                     MSDALRICREEADIDVIVITGAGDKAFCSGGDQNVKGRGGYIGKDGVPRLSVLDVQKQI
                     RSIPKPVIAAVNGFAIGGGHVLHVVCDLSIASENAIFGQTGPRVGSFDAGFGASYLARV
                     VGQKKAREIWFLCRKYNAQEALDMGLVNKVVPLEQLEDEYVQWAEEMMLLSPLALRMIK
                     AGLNAELDGQAGIQELAGDATLLYYLTDEAQEGKNAFLEKRKPNFKKYPKFP"
     CDS             5457..6329
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MR_MLE_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg157_6"
                     /sec_met_domain="MR_MLE_C (E-value: 8.6e-27, bitscore:
                     83.3, seeds: 401, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDCKIDIIPRVLHFKQPAGTSRGTYTTRKVWYLHFTAPEFPNWVG
                     IGECAPLPNLSCDDLPDYEEVLAKICRQVENQGGKLDMEALCKYPSILFGLETAIRHFF
                     EGSWALWDTPFSRGEAGIPINGLIWMGDFNRMLAQIEKKMEAGFRCIKLKIGAINFEEE
                     LALLQHIRSHYSSKEIELRVDANGAFSPTDAMEKLKRLSELDLHSIEQPIRAGQWEEMA
                     RLTSESPLPIALDEELIGYNTWEEKQRLLSAIRPQYIIIKPSLHGGLAGGEEWIAEAEK
                     LNIGXXXGV"
     CDS             6471..7574
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025:AMP-binding enzyme (Score: 52.9; E-value:
                     7.5e-17)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg157_7"
                     /sec_met_domain="AMP-binding (E-value: 1.1e-17, bitscore:
                     52.9, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIFDRQKQRLLLEGKEYTPGDIHSLVAEGEGNHPSAIWDLYLFLN
                     EWFNDDPVITVHTSGSTGAPKELLVRKDQMIQSARLTCEFLDLKQGETALLCMNLRYIG
                     AMMVVVRSLIAGLNLIVRRASGHPLADVDTPLRFAAMVPLQVYNTFQIPEEKEKLKQTE
                     ILIIGGGAVDKALEEKIRNLSNAVYSTYGMTETLSHIALRRLNGAAASDRYYPFSSVEL
                     FLSSENTLMINAPLVCDDTLQTNDIARIYPDGSFTILGRKDNVINSGGIKVQAEEIERL
                     LQSSIPVPFVITSVPDRRLGQAVTLLIEGQMEISALGTKLESVLASYYRPKYIYTVNHI
                     PQTGNGKINRKECRVLAESLQLLGRQE"
     CDS             complement(7548..8117)
                     /locus_tag="ctg157_8"
                     /transl_table=11
                     /translation="MKISFSRQTKERAFKQLYEDYYAPFCLYAKRFVDDKEVREDIVSD
                     VFTSLWDKLDTDSFDLQSETALGYIKMCVKNSCLNFLKHQEYEWSYAENIQKKAPLYET
                     ETDSVYTLDELYRMLYETLNKLPENYRTVFMKSFFEGKTHAEIAEEMNLSVKSINRYKQ
                     KTMELLRNELKDYLPLFLLFLSPEQL"
     CDS             8342..9124
                     /locus_tag="ctg157_9"
                     /transl_table=11
                     /translation="MENMNPESLLRKAQALGEDIKEMESIDVRGAYQQAQTQIKTNRRR
                     SMYNQLMRYAAFLTIPLLLSSLILGYLYWGATDTEEKYAEVMAATGSVIRYELPDHSVV
                     WLNSGSTLRYPTVFKKDNRNVELKGEAYFEVEADRKRPFYVNTPAGLSVYVYGTKFNVN
                     AYEDDNSIETVLEKGKVNVISPDGKTTVQLAPGERLLYNKVDQKLLKGKVDVYEKVAWK
                     DGKLIFRNAELGEIFKRLARHFNVDIQFNNISGKEYKYR"
ORIGIN
        1 tacacgacgc tcttccgatc tgaaaaagac tggtatgact ccttacgaat ttaaaaaaac
       61 gcaggaatcc ttaggttgat tttggctttt tcgataaaca attttccgtt ttatcggttt
      121 attcttatga taactaaata taaacggaaa tggtgcaagt taggataaag cgagtgtacg
      181 aagacttttc agagaccgac ggatatcggg tgctggtaga taaactctgg ccacgtggaa
      241 tcaaaaaaga atggttgaaa tatgattact gggcaaaaga tataacgcct tccgcagcac
      301 tgcgcagatg gtttcatgaa gatattccgg gtcactggaa tgattttgtc gtaatgtatc
      361 agaaggaact ggaagcttct caagctgttg ccgattttct gactcttatc aaacctcatc
      421 cggtgatcac tttgctttat gcatccaaag aaccggttta taatcatgcc cgaatccttc
      481 gtgattattt ggaaatgcat ctgaaagagt gaaattaaag taatttttat ctctttccgc
      541 tttcagtcgt tcactcaatt tcctaccttt gtaatctaaa tcaaaatcgt gtgtccatga
      601 agtataaatt attagttctt gatgtagacg gaacgcttct caatgatgca aaagaaatta
      661 gtaaacgtac attggctgct ttgctgaaag ttcagcagat gggagtacgt atcgtgttgg
      721 catccggccg accgacttac ggtctgatgc ctttagccaa atccctagaa ctcggaaatt
      781 atggaggtta tatcctttct tataatggtt gccagataat taatgctcaa aacggagaaa
      841 tactgtttga gcggaggatt aatccggaaa tgttgcctta tctggaaaag aaggcaagaa
      901 aaaataattt cgcactgttc acttaccatg atgatacgat cattactgac actccggaga
      961 atgaacatat ccagaatgaa gctcgcttga ataatttgaa agtgatcaag gaagaggaat
     1021 tttctgtagc aattgacttt gctccgtgta agtgtatgct tgtcagcgat gacgaggaag
     1081 cgttggttag tctggaagga cactggaaga gacggttgaa cggagctttg gatgttttcc
     1141 gttccgaacc ttattttctg gaagtagtgc cgtgtgcgat tgataaagcc aacacattgg
     1201 gagctttatt ggaagaactg gatgtgaagc gtgaagaagt catcgctata ggagacggtg
     1261 tgtgcgatgt aacgatgatt cagctggcag gactgggagt ggcgatgggg cattcgcagg
     1321 attcggtaaa ggtctgtgcg gattatgtga cagcttccaa tgaggaagac ggagtggctc
     1381 tggctgtcga aaaggcgata atagcggaag ttcgcgctgc agagattcct ttggaccagt
     1441 tgaatgcaca ggcgcgtcac gcattgatgg gtaatctggg tatccaatat acatatgccg
     1501 acgaagaccg tgtagaggcg acgatgccgg tagatcaccg tacccgtcag ccgttcggta
     1561 ttttgcacgg tggagctacg cttgcattgg gagaaacagt tgccggactc ggctcgatga
     1621 ttctctgtca gccggatgaa attgtggtag gaatgcaggt cagcggaaat catatatctt
     1681 ctgcgcacga aggtgataca gtacgtgcag tagcgacgat tgtacacaaa ggacgttcct
     1741 cccatgtatg gaatgtggac gtttttactt caaccaataa actggtgtcc tctatacggg
     1801 tggtcaacag tgtgatgaaa aaaagatgat tgacgaaaaa agtaacttga caactattga
     1861 tgcacttatt cagcggaaac agccctttgc tgtttatcgt gttcccgggg agaagtatcc
     1921 tcgtctgttg acagaagatg tcggagctgt ccgcttgatt ttcgatttga aagaactgaa
     1981 tggacaaaga ggatttgtga ttgctccgtt ccgtatagat aagtcatgtc cgattgtgtt
     2041 gatacagtcc gacaggactg gacaaccgtt gcctatggaa atcgtggcag aggaagagca
     2101 ggacttgcaa agttatccgg aggaatcttt tcataccctt tgtactggga agtatgcgac
     2161 ctgttttcat acatttattg aagccttgcg tgatgctacc tttgataaac tggtgctttc
     2221 ccgtagtctc actattggaa agaatccgga gttttcccct tcagctgttt ttcgtgcagc
     2281 ttgtcaacgt tatattcatt cctatatcta tctgtgttat actcctcaga cgggcgtgtg
     2341 gctgggaagt acccccgaaa ttatattgtc gggcgaaaaa aatgaatgga acacagttgc
     2401 cctggcagga actcaacctt tgcagaatgg taaacttccg caagtatggg atgacaaaaa
     2461 ccgtcaggag caggattatg tggcgtccta tatccgtcgg cagcttcttt cgctgggcat
     2521 tcgctccaca gagagcggac cttatccggc ttatgccggt gccttgtcac atctgaaaac
     2581 tgatttccat ttctctttga aagataataa aaatctggga gaccttctga aagtcctgca
     2641 tccgactcct gccgtgtgcg gactgcctaa agaagaagcc taccggttca ttttggaaaa
     2701 tgaagggtac gatcgtaagt attattccgg ttttatcggt tggctcgatc cggagggacg
     2761 cacagatttg tatgtgaatc tgcgttgcat gcacattgaa gacgagcaat tgacactgta
     2821 tgccggtggc ggattgttag cctcttcaga gttgaatgat gaatggcagg aaacggaaaa
     2881 gaaattgcag accatgaggc gtattcttgt gtctgctcca ataatgatga atcactaaca
     2941 agctaatcac tatcaatatg tattcggaca agaaaaatat actccagttg gtcgcgctgc
     3001 ttgaggcgca tggaattacg aaagtagtgt tatgtcccgg tagccgtaat gcacctattg
     3061 tgcatacgct gtctactcat ccgggtttta cttgttatgc tatgacggat gaacggagtg
     3121 ccggttattt tgcgattggt cttgcgctga atggcgggca tcctgctgcc gtttgctgta
     3181 cttccggtac agcgttgctc aatcttcatc ctgctgtggc agaagcgtat tatcagaata
     3241 tacctttagt tgttatctct gccgatcggc ctgctgcctg gattggacag atggcgggac
     3301 agacattgcc gcagcccggt gtttttcaga cattggtgaa gaagtcggta aaccttccgg
     3361 agattcagac agaagaggat gaatggtact gtaaccgctt ggtgaatgaa gccttgctgg
     3421 aaaccaacca tcatggaaaa ggtccggtac atattaatat tcctatatcc gaacctttgt
     3481 tccagtttac ggtggagtcg cttcccgaag tacgtgtcat tacgcgctat caggggctga
     3541 atgtatacga ccgggattac aatgacctga tcgaacgctt gaacagatat cagaagcgga
     3601 tgattattgt cggacagatg aacttgattt acttgtttga gaaacggcat accaaattgt
     3661 tatataaaca tttcgtgtgg ctgaccgagc atatcggtaa tcagacagta cccggtattc
     3721 cagtgaagaa ttttgatgcg gcgctttatg cgatgcccga agagaaaacc ggccagatga
     3781 ctcccgagtt attgattact tatggcggac atgtggtttc caagcggttg aaaaaatatc
     3841 ttcggcagca tccgcctaaa gaacattggc atgtgtctgc tgatggagaa gtggttgacc
     3901 tttacggttc tctgactacc gtcatcgaaa tggacccgtt tgagtttctg gagaaaatag
     3961 ctccattgct ggataaccgc gtgccggagt atccccgtgt atgggagaat tattgcaaga
     4021 ccattcccga accggagttc ggatattcgg aaatgtcggc tatcggtgct ttgataaaag
     4081 cattgccgga atcatgtgca ttgcatctgg caaatagttc tgttatccgc tatgctcagt
     4141 tataccaggt tccttctacg attgaggtat gctgtaatcg gggtacaagc ggcatcgaag
     4201 gctcgttgtc cacggcagtg ggatatgccg ccggttcgga taaactaaat tttatagtta
     4261 taggtgacct tagtttcttt tatgatatga atgctttgtg gaacatcaat gtccgtccca
     4321 atctacgcat tcttttgctg aataacggag gaggggagat tttccataca ttacccggac
     4381 tggatatgtc gggtacatcg cacaagtata ttacagccgt tcacaagaca tctgccaaag
     4441 gatgggcgga agaacgcgga ttcctttatc aacgggtaga gaatgaagaa cagcttgccg
     4501 aagcgatgaa aaccttcaca caaccggaag ccatggaaca accggttctg atggaagtat
     4561 tcagcaataa aaataaagat gcacgtatac tgaaagatta ttatcatcaa ctaaaacaaa
     4621 aatagattat gtcaacacaa agagaatgga caaccatcag agaatacgaa gatatcctct
     4681 ttgattacta taatgggatt gcccgcatca ccattaaccg tgaacgctat cgaaatgcat
     4741 ttactccgac tacaacggct gaaatgagtg acgcattgcg catttgccgg gaagaagcgg
     4801 atattgatgt gatcgtgatt acgggagctg gtgacaaggc tttctgttcg ggcggtgacc
     4861 agaatgtgaa agggcgtggc ggctatatcg gcaaggacgg tgttccccgt ctgagtgttc
     4921 tggatgtaca gaaacaaatc cgcagcattc cgaaaccggt aattgctgcc gtaaatggat
     4981 ttgccattgg tggcggacac gtgcttcatg tagtttgcga tttatcgatt gcttcggaga
     5041 atgcgatctt cggacagacc ggtcctcgcg taggtagctt tgatgcaggt ttcggagcct
     5101 cttatctggc tcgtgtcgtc ggtcagaaga aggcacgtga gatatggttc ctttgccgga
     5161 aatacaatgc gcaggaagcg ttggatatgg gattggtgaa taaagtagtt ccattggagc
     5221 aactggagga tgagtatgtg caatgggcgg aagaaatgat gctgctcagt ccgttggcct
     5281 tgcggatgat taaagccgga ttgaatgccg aactggacgg ccaggcaggt attcaggagc
     5341 tggcagggga tgcgaccttg ctttactatc tgacggatga ggctcaggaa ggaaagaatg
     5401 catttctgga gaaacgtaaa cctaacttta agaaatatcc gaagttccct taaacaatgg
     5461 attgcaaaat agacattatt ccgcgggtgc ttcatttcaa gcagccggcc ggaacttcac
     5521 gaggcacata cacaacgcga aaagtctggt atcttcattt tactgctccc gaatttccca
     5581 attgggtagg gatcggggag tgcgctcctc tgccaaatct tagctgtgat gatcttcccg
     5641 attacgagga agtgctggct aaaatctgcc ggcaggtaga gaatcaggga ggtaagctgg
     5701 acatggaagc tctatgcaag tacccttcca tcctttttgg cttggagaca gccattcgtc
     5761 acttttttga aggaagctgg gctttgtggg atacaccctt ctcacgtggg gaagcaggta
     5821 ttccgataaa cgggcttatc tggatgggtg acttcaatag aatgcttgct cagatagaga
     5881 agaagatgga ggcgggattc cgctgtataa agttgaagat cggtgccatc aactttgaag
     5941 aagaactggc tttactccaa catatccgct ctcactattc ttctaaagaa atagaattgc
     6001 gtgtagatgc caacggagca ttctcaccaa cagatgcgat ggagaaattg aaacgcttgt
     6061 ccgaactgga cttacattcg atagaacagc ccattcgtgc cggacaatgg gaagaaatgg
     6121 cacgtcttac ttccgaatct cctttgccga tagctttgga tgaggaactg ataggctata
     6181 atacctggga agaaaagcaa agattacttt cggctatccg tccgcaatat attattatca
     6241 aaccttctct tcatggcgga ttagccggtg gcgaggagtg gattgcagaa gcggagaaac
     6301 tgaatatcgg nnnnnnnnnn ggagtctaat atcggtctga atgctatcgc tcaatggtgt
     6361 gctacttttc agaatccatt gccgcaaggg ttaggcaccg ggttactctt tacagataat
     6421 gtagagatgc ctcttgaaat cagaaaagat tgtttgtggt tttgtaaatg atgatattcg
     6481 accgacaaaa acagcgtttg ttgctggagg gaaaggaata tactccgggc gatatacata
     6541 gccttgtagc agaaggtgag ggaaaccacc cttctgctat atgggacttg tatctttttc
     6601 taaacgaatg gtttaatgat gatcctgtga tcactgtaca tacttccggt tctacggggg
     6661 cacctaaaga attgcttgtt cgcaaagatc agatgataca gagtgcacgc ctgacttgtg
     6721 agtttctgga tttaaagcag ggtgaaacgg cattattgtg tatgaacctt cgctatatcg
     6781 gggcgatgat ggtagtcgtc cgttctttga ttgccgggtt gaatcttata gtacgtcggg
     6841 cttccggtca tccattggca gatgtggaca ctcctttaag gtttgcggct atggtgcctt
     6901 tgcaggtgta taatacattt cagatacccg aagagaaaga gaaattaaag caaacggaga
     6961 ttttaattat aggaggcgga gcagttgaca aggctttaga ggaaaaaatc agaaatctgt
     7021 ccaatgctgt ttattcaact tacgggatga cggaaaccct ttctcacatc gctcttcgcc
     7081 gtctgaatgg cgcagcagca tccgatcgtt attatccttt ttcatccgta gagttgtttt
     7141 tgtcttcaga aaatactttg atgattaatg ctccgcttgt ttgcgatgat actctgcaga
     7201 caaatgatat tgcccgcatt tatccggacg gtagttttac gattctggga agaaaggata
     7261 atgtaatcaa tagcggagga attaaagtgc aggcagaaga gatcgaaagg ttacttcagt
     7321 cgtctatccc tgtaccgttt gtgatcactt ccgttccgga tcggcgtttg gggcaggctg
     7381 ttactttatt gatagaaggg cagatggaga taagtgcgct agggactaaa ttggaatcag
     7441 tcttagcatc ttattatcgt cctaaatata tttatacggt aaatcatatt cctcaaacgg
     7501 gaaatgggaa gattaatcgt aaagagtgcc gtgttttagc agagagctta cagttgctcg
     7561 ggagacagga ataataaaaa cagcgggagg taatctttca actcgttccg caggagttcc
     7621 attgtttttt gtttataccg attaatggat tttacgctga gattcatttc ttcggcaatt
     7681 tccgcatgtg ttttgccttc aaagaaactt ttcataaata cggtgcgata gttttccgga
     7741 agcttattta atgtttcgta tagcattcgg tataactcat caagtgtata tacactatct
     7801 gtttctgttt catatagagg agctttcttt tggatatttt cggcataact ccattcatac
     7861 tcctgatgtt tcagaaagtt caaacagctg ttttttacac acatttttat atatcccaat
     7921 gctgtctctg attgcaaatc gaaagagtcg gtatccaact tgtcccatag ggaggtgaat
     7981 acatccgaaa caatgtcctc gcggacttct ttatcatcta caaatctttt tgcatacagg
     8041 cagaaagggg cataataatc ctcgtaaagt tgtttgaaag ctctttcttt agtctgtctg
     8101 gaaaaagata ttttcattat tttgagattc tgtagttttt cattttactt caaatatata
     8161 ataagaaaat cagcatttcc taaaattttc tttctttttt cgtttttggt atgaagctgt
     8221 ggaaaataag atagcttaaa aaataaaata aaaaaaagta tgttatacct gtcccttttc
     8281 gattacctca gttgtactat tagtgtaatt gcaattaaaa gataagattt tgaaagagaa
     8341 tatggaaaat atgaatccgg aatcattgct ccgaaaagcg caggcattag gagaggatat
     8401 taaggaaatg gagtctattg atgtaagggg tgcctatcaa caagcacaga cccaaataaa
     8461 gacaaacaga agaaggagta tgtataatca gttgatgcgt tatgctgcat tcttgactat
     8521 acctttatta ctttcttctt tgattttggg atatttatat tggggagcaa cggatactga
     8581 agagaagtat gcagaagtca tggcggctac aggttcagtc attcgttacg aacttcctga
     8641 ccattcggtt gtctggttaa actccggtag tactttacgt tatcctaccg tttttaagaa
     8701 agataatcgt aatgttgaat taaaaggtga agcctatttt gaagtagaag cggacaggaa
     8761 acggcctttt tacgtcaata ctcctgccgg gttaagtgtc tatgtgtacg gaaccaaatt
     8821 taatgtgaat gcttacgaag atgataatag tatagaaacg gtattagaaa agggtaaagt
     8881 aaacgttatt tcaccggacg gaaaaacaac agttcagtta gctccaggag aacgattgct
     8941 ttataataag gtagatcaga aattgttgaa gggaaaagtg gatgtttatg aaaaggtggc
     9001 atggaaagac ggtaagttga tattccgcaa tgccgagttg ggtgaaatat tcaaacggct
     9061 ggcacggcat tttaatgtag atattcagtt taataatata tcgggaaaag aatataaata
     9121 tcgt
//
