LOCUS       c00184_gnlPro..         7538 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MDGKNFLA_184.
ACCESSION   c00184_gnlPro..
VERSION     c00184_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 03:55:47
            Original ID  :: gnl|Prokka|MDGKNFLA_184
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7538
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7538
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[274:3245]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      275..3245
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7538
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7538
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             275..2503
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg184_1"
                     /sec_met_domain="Gly_radical (E-value: 5.6e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     KFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2517..3245
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.5;
                     E-value: 1.4e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg184_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.7e-29, bitscore:
                     92.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(3238..3636)
                     /locus_tag="ctg184_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDKDTVGAHARGYNAISLGVCYEGGLDENGKAXXXXRYPEAKVVGHRDLSPD
                     TNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(3623..3790)
                     /locus_tag="ctg184_4"
                     /transl_table=11
                     /translation="MKEIVSRILDVIMYLIPFFGKRKRDRVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             complement(3850..4353)
                     /locus_tag="ctg184_5"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLILRPGKTLAELMKILSYQQVQTRAVAQAGAGDGGGEDPDKNPDSGGDGSGDEEAP
                     DPTV"
     CDS             4547..4777
                     /locus_tag="ctg184_6"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             4864..6978
                     /locus_tag="ctg184_7"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GEKAANEKKKELPAIAFSASYRGRRTKVNLVKYLGHIVIDIDHLSKEELARILPIIKRC
                     DYTRIAFISPKGMGVKIIVRACHPDETLPETLQEIEDFHHAAYTRLVSFYTELCQIEID
                     TSGQDVARTCLFSYDPEIYFNPNADAFLVDQPQASYKVSNRKNLSGSKQQTPPDGPPTN
                     EDTALNAHSANASLVLTLTYYHNKSEKYIVGNRNNYLHHLSCTFNRYGIPQEEASAFIK
                     SQFTDFPADETASLINSAYAHTDEFNTCKLNGTQKRILRIEQYISEHYETRYNEVLHIM
                     EYRRRHPDTEKPEPFRILDEMMENSIWIEINELGYACTVKTIENLIYSDFSQSYHPIRE
                     YFELLPEWDGTDYIRILADSVQTSHQEFWAECLERYLVAMCAAATQENIVNHTVLLLCS
                     EIQNIGKTTFINNLLPPELRAYLSTGLINSNNKDDLAKITQAMLINLDEFEGMSGRELN
                     AFKDLVTRKVISFRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHPYSIQF
                     ITINYAQLYAQIKYLLNKPGYQYWFTQADNERLEENNEAFIFHSPEEEMVLTHIRKPER
                     FEKVYYLTVSEIAELIRERTGYQYSIGSKIQLGKVMTKHHFESKKGNNGRRYAVFIIDI
                     EQVKSNRLYE"
     CDS             complement(7048..7353)
                     /locus_tag="ctg184_8"
                     /transl_table=11
                     /translation="MEAKTNEEFFNVSALIDERFGKEGTASRAEAEEKAYAFYTGQIIE
                     DARKKAKITQAELARRIGSDRSYISRVESGQTEPKVSTFYRIMNALGCKIEFSMIL"
     CDS             complement(7334..7537)
                     /locus_tag="ctg184_9"
                     /transl_table=11
                     /translation="FMRLIEEGLYELRIEYQSNIYRIFFCFDEGRIVILFNGFQKKTEK
                     TPKKEIDKAKILRKEYYGSKNK"
ORIGIN
        1 agaccgccgc aatcgaccac tctgccacct ctccaaaact tccttatcag aagtgctttt
       61 cgttaaaagc ggtgcaaagg tacgaagata ttttaaactt gcaagagttt tcactaaaaa
      121 tttaatctaa tcttttccta aggatagcaa ttaataggta ttttatgttg aaaagcataa
      181 aatagagcat atttatgctg attatatgaa aaatgattgt aactttgtgc gctctaaaga
      241 ataggataac tacaacctct aattttatca ttttatggaa ttaaacaaga tctttaaaga
      301 cggtctttgg agcagcgaaa tcaacgtcag agatttcgta agtcataaca tcactccgta
      361 ttacggagat gcttcattcc tcgaaggacc tacagaacgt accaaagccg tatggaaccg
      421 ctgcctcgaa gcactggcag aagaaagaga aaacaacggt gtccgctcat tagataatgt
      481 taccgtatca accatcactt cccacaaggc cggatatatc gacaaggaaa acgaactgat
      541 cgtcggcctg cagacagacg aacttctgaa acgtgccatc aagcctttcg gaggtatcaa
      601 cgtagtcagc aaagcctgtc acgaaaacgg cgtggaagtg gacgaccgcg taaaagatat
      661 tttcactcac taccgcaaga cgcacaacga cggagtattc gacgtatata cggaagaaat
      721 ccgttccttc cgttcgctgg gattccttac cggacttccc gacaattatg cacgcggacg
      781 catcatcggt gactaccgcc gtatggcact ttacggcatc gaccgtctga tcgaagcaaa
      841 gaaggaagat ttgcacaacc tcaccggtcc gatgacagag gcccgcatcc gcctgcgcga
      901 agaagtggca gaacagatca aggcactgaa agacatgaaa gtgatgggcg aatactacgg
      961 tctcgacctg agccgtcccg cttacacggc acaagaagcc gtacagtggg tatatatggc
     1021 ttaccttgcc gccgtcaaag aacaagacgg tgccgccatg tcactgggta acgtttcttc
     1081 tttcctcgat atctatctgg aatatgaact gagcaaagga accatcaccg aatcattcgc
     1141 gcaagagctg atcgaccagt ttgtcatcaa actgcgtatg gtccgccacc tgcgtatgca
     1201 atcatacaac gacatctttg caggcgaccc gacctgggta accgaatctc tgggcggacg
     1261 tctcaacgac ggacgtacca aagtgacaaa gacctccttc cgtttcctgc agacattgta
     1321 caacctcggc ccttcaccgg aaccgaacct gaccgtactc tggagcccgg aacttccgga
     1381 aggattcaaa gaattctgtg caaaggtttc tatcgacact tcttctatcc agtacgaaaa
     1441 tgacgacctg atgcgcgaag tacgtcagtc cgacgactac ggaatcgcct gctgcgtatc
     1501 ataccaggaa ataggtaagc agattcagtt cttcggtgcc cgttgcaacc tggccaaagc
     1561 cctgttgctt gccatcaacg gcggacgttg cgagaatacc ggtacagtca tggtgaagaa
     1621 catccccgta ctgaccagtg acacgctgaa gttcgaagaa gtaatggaca actacaaaaa
     1681 agtgctgacc gaaatcgccc gcgtatataa cgaagccatg aacatcatcc actatatgca
     1741 cgacaagtat tactacgaga aggcccaaat ggctcttgta gatacgaacc cgcgcatcaa
     1801 cctagcttac ggtgtagccg gactttccat cgccctcgat tcactgtctg ccatcaaata
     1861 tgcgaaagta actgcccgcc gcaatgatat cggtctgaca gaaggcttcg acatcgaagg
     1921 agaattccct tgcttcggta acgacaacga caaggtagac caccttggcg tagacctggt
     1981 atacttcttc agcgaagaat tgaagaaatt gcctgtttac aagaatgccc gtcctaccct
     2041 ctccttgctg actatcactt ccaatgtgat gtacggcaaa aagaccggtg ctactcccga
     2101 cggacgtgcc aaaggtgttg ccttcgctcc gggtgccaac ccgatgcacg gacgcgacaa
     2161 gaatggtgcg atcgcttctc tgagttctgt agcgaaactt cgttaccgtg actcacagga
     2221 cggtatcagc aacaccttct ctatcgttcc gaaatcactg ggtgctacgg acgaagaccg
     2281 tatcgagaac ctcgtaacga tgatggacgg ttacttcacc aaaggtgccc accacctgaa
     2341 cgtaaacgta ctgaaccgtg atatgctgta cgatgccatg gagcatccgg aaaactatcc
     2401 gcagctgact atccgcgttt ccggctatgc cgtaaacttc gtgaagctga gccgcgaaca
     2461 ccaactggaa gttatcagcc gtagcttcca cgaacgtatg taatctatta ctctatatga
     2521 tgataaacgt acattcatac gaaagtatgg gaacattcga cgggccgggc ttacggctcg
     2581 tcgtttttct tcaaggatgc aacttccgtt gtctctattg cgccaatccg gatacgatag
     2641 caggaaaggg aggcacgcct accccaccgg aagaaatcgt ccgcatggca atgagccagc
     2701 gccccttctt cgggaaacga ggaggaatca ctttctccgg aggcgaaccg acgtttcagg
     2761 cgaaagcact cgtcccgctg gtccgcgaac tgaaagaaaa aggcatccat gtctgtctgg
     2821 acagcaacgg aggactttgg aacgaagacg tagaagaatt attcagactg accgatcttg
     2881 tgttattgga tataaaggaa ttcaacccta accgccatca aacgctcacc ggaagaagca
     2941 acgagcaaac catccgcacg gccgcctggc tggaagaaca ggagaaacct ttctggctac
     3001 gctacgtgct agtgcccgga tacagcgatt ttgaagaaga catacgcgca ctgggagaag
     3061 cactcggaaa atacaagatg atccaacggg tggaaatact gccttaccat acactgggcg
     3121 tacataagta cgaagcgatg ggacaggaat acaagatgaa aggggtaaaa gaaaataccc
     3181 ccgaacagct tgaaaaagct gcggaggtat tcaaagagta tttcactacg gtggtagtta
     3241 attaatggag aattctccaa tgacctcaaa gcaagggcat tctttaatcc gttcccaagg
     3301 atctacgatc ccattatagt tggtgtcggg acttaaatcc cgatgcccta ctactttagc
     3361 ttccggataa cgcnnnnnnn nnncagcttt tccattctca tccagaccgc cctcataaca
     3421 tacacctaag ctgatcgcat tatagccacg tgcatgagct ccgaccgtat ctttatcccg
     3481 catcggctca atctcgcccg acttgcgtat ataataatga tacccccagc acgaaaagcc
     3541 acgataacgg tgagccgtat ccacatcctt tgccgtaaag tcgcaatcga cacgggtggc
     3601 ggaacaatgg atcacaatca gactaatctt tctcatgttc cttttccctt tccgtcgttt
     3661 tcactttgca cacctcctta caagtagtat gatagcgcac ttctctcacc accctgtccc
     3721 gctttctctt tccgaagaaa ggaatcagat acatgatcac atctaaaatc cgacttacta
     3781 tttctttcat cttatcaaaa tattaatgga agttattcta attcgttatt accttgaccc
     3841 gtctgccaat tataccgtag ggtccggagc ctcttcatca ccggagccat caccaccgga
     3901 atccggattc ttgtcagggt cttctccccc gccatcgcct gcacccgcct gcgccaccgc
     3961 acgagtctgc acttgctgat aactcagaat cttcatcaac tccgccagcg tcttaccggg
     4021 gcgtaaaata agatgcgccc ccttaatcat tgaaccggaa aattccttga ccgatgccgc
     4081 accttcactg ttgagtccta tctgcaaact gcccaaatcg cccagtctcg caattcttcc
     4141 tgccttcaac gcacgcttca cttcgtaaag aatacccgaa atagccgcac gtacatccgt
     4201 ttccgtacaa gtagaacgac tggtgatcgc ttcgcacaac tcttcaaagt ccaactctcc
     4261 cgacgcctgc gcctgtgcat aatactttgc cggttcgctc ttttttgcag gattcttgcg
     4321 cggcaccact gaataagata ctgtcattgc cataataaaa taattttaaa gtttataata
     4381 cataggatgc atccccttct tgttaacagc acaaagataa ggatagccgg acaagccata
     4441 ttccccaacg aagtttcttg attcccaacg aataaaaacg aagtcaaacc tttgaataac
     4501 aaggaatttt atgtaacttt atagcataaa acaagaagaa aacatcatga attacaaaga
     4561 agattctttc cttatccgca cctataccaa agcggaactg gctcaccttt acaatcccca
     4621 tgtctgcctc aaagttgctt tacaaatact tcgtcgttgg attatttaca acctccctct
     4681 cctgcacgaa ctcgaacagg aaggctaccg cgcacgcaac cgtctccttt cgccgaaaca
     4741 agtggcaact atcatacact ttttgggcga accgtagtaa cttattgcca ttttatacaa
     4801 agttccttta cacatttccg caaagaagtt cctttgcatc gacaaacaat aatcaaaata
     4861 aaaatgaaac atttcaccat atttcaaggt ttctactacg caatagcaga gatgaccgaa
     4921 gaagaaatag tgtccactat cggcagtttt acctaccggg aaaaagtcga agaaatacgc
     4981 cgcatctttg cagagcaagg agaaaaggct gccaatgaga agaaaaaaga attgccggca
     5041 atcgccttct ccgccagcta ccgaggaagg cgtaccaaag tgaacctcgt caaatatctg
     5101 ggacacatcg tgatagacat cgaccacctc agcaaagaag aattggcacg catcctccct
     5161 atcatcaaga gatgcgatta cacccgcatc gcctttatca gccccaaagg gatgggcgtg
     5221 aaaatcatag tacgcgcatg ccatccggat gagacattgc ccgaaaccct tcaggaaata
     5281 gaagacttcc atcatgcagc ttacaccaga ctcgtttctt tttacaccga actctgtcag
     5341 atagaaatcg acacctccgg tcaggatgtg gcacgcactt gtctgttctc ttacgatccg
     5401 gagatttatt tcaatccgaa cgctgacgct tttcttgtag atcaacctca ggcatcctac
     5461 aaggtttcaa atcgtaaaaa tctctccggc agtaaacaac agactcctcc ggacggcccc
     5521 ccgacgaatg aagacacagc gctcaacgcc cattccgcca acgcttctct ggtgctgact
     5581 ttaacctact atcacaacaa gtccgaaaaa tacatagtgg gcaaccggaa caattatctg
     5641 caccatctct catgtacctt caatcgctac ggcattccgc aggaggaagc atccgccttt
     5701 atcaaaagcc agtttacaga ctttccggcg gacgagacag cctctttgat caatagcgcc
     5761 tacgctcata ccgacgaatt taatacctgt aagctaaatg gcacgcagaa acgaatatta
     5821 cggatagagc aatatatcag cgagcactac gaaacacgct acaacgaagt gctgcatatc
     5881 atggaatatc gcagaaggca tcccgacacc gagaaaccgg agcctttccg catcttggat
     5941 gagatgatgg aaaacagtat ctggatagaa atcaacgaac tgggatatgc ctgcactgtc
     6001 aaaacaatcg aaaatctgat ttactccgat ttcagccagt cgtatcatcc cattcgcgag
     6061 tattttgaat tactgcccga atgggacgga accgactata tccgtatcct ggcggatagt
     6121 gtccagacga gtcatcagga gttttgggcg gaatgcctgg aacgttatct ggtggcgatg
     6181 tgcgcagcgg ctactcaaga gaatattgtc aatcacacgg tacttctgct atgcagcgag
     6241 atacagaaca taggaaagac tacctttatc aataatctcc tgcctcccga actgcgtgct
     6301 tatctctcta ccggactaat caattcgaac aataaagatg atttagccaa aataacgcag
     6361 gccatgctga tcaatctcga cgagtttgaa gggatgagcg gtcgggaact gaatgcattc
     6421 aaagatttgg taacgcgcaa agtgatcagt ttccgtctgc cttatgcacg ccgttcgcaa
     6481 aacttccctc atacggcctc ttttgcagga acctgcaatt atcaggaagt gcttcacgac
     6541 acgacaggca accggcgttt cctttgtttt catccttatt ccatccaatt tattaccatc
     6601 aattatgcgc agctctatgc tcagatcaaa tatctgctga ataagccggg atatcaatac
     6661 tggttcacgc aagcggacaa tgaacggctt gaagagaata acgaggcttt catctttcat
     6721 tcaccggaag aagaaatggt tcttacgcat atccgcaagc cggaacgttt cgagaaggtt
     6781 tattatctga ctgtcagtga gattgcggaa ctaatacgtg agcgtaccgg ttatcagtat
     6841 tccatcggat caaaaataca gttggggaag gtgatgacta agcaccattt tgaatctaaa
     6901 aaaggaaata acgggcgccg atatgccgta tttattatag atatcgagca ggtaaaatca
     6961 aacaggctgt atgaatagaa gcgggaaagc cttgtgaata tagcaaaatg cagagtcatc
     7021 ttgacagata ctctgcattt attgttttta caaaatcatt gaaaattcga ttttacagcc
     7081 taacgcattc attatacgat agaaagtaga tactttaggc tctgtctgac cactttctac
     7141 tcttgaaata taagaacggt cagagccgat acgccgggct aattcagcct gagtgatttt
     7201 agcctttttt cttgcgtcct cgataatctg tcctgtataa aaggcgtagg ccttttcttc
     7261 tgcctctgca cgagaagctg ttccttcctt accaaaacgc tcatctatta atgcactgac
     7321 attaaagaat tcctcatttg tttttgcttc cataatactc ctttcttaat atctttgctt
     7381 tatcaatttc tttctttggt gttttttcgg ttttcttttg aaaaccgtta aacagaatca
     7441 caatacgccc ttcgtcgaaa caaaagaata ttctatatat attgctttgg tactcgatac
     7501 gaagttcata aagtccttct tctatcaacc gcataaat
//
