LOCUS       c00225_gnlPro..         3684 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PBNBGFKC_225.
ACCESSION   c00225_gnlPro..
VERSION     c00225_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 04:07:07
            Original ID  :: gnl|Prokka|PBNBGFKC_225
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3684
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3684
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[250:3314]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      251..3314
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3684
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3684
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             251..2518
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg225_1"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-46, bitscore:
                     145.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 6.9e-283, bitscore:
                     927.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISIVKDLKESTALPKEWQGFKPGAWTESIDVRDFIQHNYTPYSG
                     NEEFLSGPSQRTLRLWDELKVLLKREIDNGGVLDADEEVVSSITSHKPGYIDKELEVVV
                     GLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYTSDI
                     RAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDRLIAERRKDLKNREHSPLTDELIRL
                     REEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGR
                     VSTFFDIYFTRDLEQGLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTES
                     IGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSS
                     VQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGK
                     QVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHD
                     PEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVD
                     DIACSLVSNFMEKLRKLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGA
                     NPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAY
                     FAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFH
                     TR"
     CDS             2535..3314
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg225_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.7e-31, bitscore:
                     96.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSAGDVLREIARYRDFLQAAGGGVTLSGGDPLFQPDFTGA
                     VLKGCRELGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPERYRSLTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEGLSRHAALLGNVERVDVLPFHQMGRFKW
                     DELKLDYTLKGTPEASAKLAEQVRGIFRREGFPDCVH"
     CDS             complement(3415..3684)
                     /locus_tag="ctg225_3"
                     /transl_table=11
                     /translation="SVMDGASVAVAVDGDFPSLCSFRGLPVVTLFSTRLPDVYRPMGTF
                     NRSLYSHQCCSPCFRKDCDRDVPCNRHIAVREVLDALREISGKE"
ORIGIN
        1 atgagctccc atgaaaaaag atgcccccct acaagactct tcagaaggcc ccattctgtt
       61 tttccataaa aaatatacat atctccgaaa tgaaccagaa aaggtaaaga acagttccct
      121 ctcccagaag aattagcctt gttgtctccc ggaattcacg atataattac gtggcgcgga
      181 gttttgccgt ttccggcctt tcaacggctg cggaacgttt ttctccggtc ttcttcacta
      241 catcctattt atgatcagca tagtaaaaga cttgaaggag tccaccgccc ttccgaagga
      301 atggcaggga ttcaagccgg gagcatggac ggagtccatt gacgtacggg atttcatcca
      361 gcataactac acgccctatt ccggcaatga agaatttctt tccggcccct cccagcgcac
      421 gctccgttta tgggatgaac tgaaagtcct gctgaaaagg gaaatagaca acggcggcgt
      481 tctggatgct gatgaagaag tggtatcctc catcacctcc cacaagcccg gctacattga
      541 caaggaactg gaagtggtcg taggcctcca gacagacgcc cccctgaaac gggccctgat
      601 gccctttggc ggcctgcgca tggcccagca ggccctggaa tcctacgggt tcaagatgtg
      661 cgagaaaacc gcggatattt tcaaaaagat acgcaagacg cacaatgaag gcgttttcga
      721 cgcctacact tccgatatcc gggcggcccg ttccgccggc attattaccg gccttccgga
      781 cgcctatggc cgcggccgca tcatcgggga ctaccgccgg gtggccctgt acggcacgga
      841 cagactgatt gccgaacgcc gcaaggacct caaaaaccgt gaacattccc cccttacgga
      901 tgagctgatc cgcctgcgcg aagaaatgag cgaacaaata cgcgccctgg aagaactcgc
      961 ccagctgggc gcctcctacg gctgcgacct cactcgcccc gccgcaaacg cccgcgaagc
     1021 cgtgcaatgg acgtacctgg gctacctagc cgccgtgaag gaacagaacg gagcggccat
     1081 gtccctgggc cgcgtttcca ctttcttcga catttatttc acccgcgacc tggaacaggg
     1141 gctcatcacg gaagaggaag ttcaggaaat catcgaccag ttcgtgatga agctgcgcat
     1201 cgtccgtttc atccgcacgc ctgattacaa taacctgttc tccggagacc ccacctgggt
     1261 gacggaatcc atcggcggca tgggagagga cgggcgcacc ctcgtcaccc gcagttcctt
     1321 ccgcatgctg cagaccctgt acaacctggg gccggctccg gaaccgaacc tgaccgttct
     1381 gtggtcccgg aacctgccgg aagccttcaa aagcttctgc gccaaggttt ccattgaaac
     1441 gtcctccgtc caatacgaga acgacgacct gatgcgcccg cactggggcg acgactacgg
     1501 catcgcatgc tgcgtgtccg ccatgcgcat cggcaagcag atgcagttct tcggagcgcg
     1561 cgccaacctc gccaagtgcc ttctctacgc cctgaacggc ggcgtggatg aactcaaggg
     1621 caaacaggtg gccccgccct ccccccgcta cacggaagaa attctcaact atgatgaagt
     1681 gatggccctt tacgacaaga tgcaggactg gctcgccaaa acttacattg acgcgctgaa
     1741 catcatccac tacatgcacg acaaatactg ctatgaacgc attgaaatgg cgctgcatga
     1801 tccggaaatc ctgcgcacga tggccacggg aatcgccggg ctttccgtgg cggcggactc
     1861 cctgtccgcc atcaagtacg ccaccgtaaa agccatccgc aatgaagagg ggctgattgt
     1921 ggacttcaag acggaagggg aattcccctg ttacgggaac aatgacccgc gcgtggacga
     1981 catagcatgc agcctggtca gcaatttcat ggaaaaactg cgcaagctcc acacttaccg
     2041 caattccctg cccacccagt ccatcctgac gatcacctcc aacgtggtgt acggcaaaaa
     2101 gacgggcaac acgccggacg gccgccgagc cggggaaccg ttcgcccccg gcgccaaccc
     2161 catgcacgga agggacagga acggagccgt ggcgtccatg ctctccgtag ccaagctgtc
     2221 ctatgacgac tccctggacg gcatctccta caccttctcc atcgttccgc aggccctggg
     2281 caaggaggaa agggaacgcc gcgtcaagct cgtctccctg ctggacgcct actttgccgc
     2341 tacaggtcac cacattaatg tgaacgtact ggaacgggaa acgcttctcg acgccatgga
     2401 tcacccggaa aaatacccgc agcttaccat ccgcgtttcc ggctatgccg tgaatttcat
     2461 caagctgacc cgggaacagc agcaggaggt catcaaccgc accttccaca cccgctaacc
     2521 cggacgacac acggatgccg caggtttttc cacaaaacca tgatccggac ggagaaagct
     2581 ccgtcaccgg tttggttcat tccgtggaat cctgcggcac cgtggacggt cccggcatcc
     2641 gctttgtcct tttcctgtcc ggctgcagcc tgcgctgccg ctactgccac aatccggata
     2701 cctcctacgt tcggcggggc cggctcagaa gcgcgggtga tgtcctcagg gaaatagccc
     2761 gttaccggga ttttttgcag gcggcgggcg gcggcgtcac cctctccggc ggggaccccc
     2821 tcttccaacc tgattttacc ggcgccgtgc tgaaaggctg ccgggaactg ggactgcata
     2881 cctgcctgga cacctccggc catctgggcg tgaatgcggg cgaagaaatg ctggcggata
     2941 cggacctggt cctgctggat atcaaggcat ggaatccgga acggtaccgc tccctgacag
     3001 gcggggaact gcgccctacg ctggagtttg cggaacgcct ggcctccctc cgcaagcccg
     3061 tgtggctgag gtatgtgctg gtacccggcg ttacggataa tctggaagaa attgagggac
     3121 tctcccgcca tgcggcgctc ctgggcaacg tggaacgcgt ggacgtgctc cctttccacc
     3181 agatggggag attcaaatgg gatgaattga agctggatta taccttgaag ggcaccccgg
     3241 aagcgtccgc aaaactggcg gaacaggtcc gcggcatttt ccggcgggaa ggctttccgg
     3301 attgcgtgca ttaaccggat tttcgcgcct tggcgccatc aaccgcagca aaccgaatac
     3361 agatatctcc cctcctcatc ttccggatga aaggcccttc tcccctttcc caaattattc
     3421 ctttccggaa atttcccgca aagcatccag cacttcccgc acggcaatgt ggcggttgca
     3481 ggggacatcc cggtcacaat ccttccggaa gcagggggaa cagcactggt ggctgtaaag
     3541 ggaacggttg aatgtaccca tagggcggta cacatccgga agccgggtgg aaaacagagt
     3601 caccaccgga agcccgcgga aggaacagag ggacgggaaa tcgccatcca cggctaccgc
     3661 caccgaagcg ccgtccatga cgga
//
