LOCUS       c00146_gnlPro..         5356 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AILKLODE_146.
ACCESSION   c00146_gnlPro..
VERSION     c00146_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 08:28:07
            Original ID  :: gnl|Prokka|AILKLODE_146
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5356
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5356
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[392:3369]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      393..3369
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5356
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5356
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             393..2633
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg146_1"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-45, bitscore:
                     142.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.2e-285, bitscore:
                     935.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGEIWKREINVRDFIQNNYKPYEGDDSFLKDSSD
                     KTKKVWDKLTEMFKVEREKGVYDAETKYPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IYPKCGTRMVENSLEAYGYKLDPMTKEIFTKYRKTHNEGVFSAYTDEMLAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTEILIAEKKHYLKRLDIQELTEEIIQSREEISEQIKAL
                     KAFEKMCAGYGFDVTKPAKDAREAVQFVYLGYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DIREGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDERSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMALHDGSVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVGFEREGDYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRNATPTQSILTITSNVVYGKKTGATPDGREGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQIANMVGLLDGYFTPDGGQHLNV
                     NVFDKELLLDAMEHPENYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             2641..3369
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg146_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.6e-37, bitscore:
                     116.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LEIKGNIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWDS
                     KGLAKYQMTPEELLAEVLRYKNFIAKGGVTVTGGEPLLQAEFLREFFRLCREAGIHTAL
                     DTSGYICTPKALAVLEYADLVLLDIKTIDPDLHPRLTAVKLDNTLRFLDELEKRGVPVW
                     IRHVIVPGLTDNDEALDKLAEYISHYTVVQKAELLPYHTMGTYKYEAQGLDYKLKEVEP
                     LSAERLANAKEIFKKHGITI"
     CDS             3458..4360
                     /locus_tag="ctg146_3"
                     /transl_table=11
                     /translation="MEQHHHDHHHHEITSLNKAFIIGISLNLAFVVAEFTAGFWYNSLG
                     LLSDAGHNLGDVASLVLAMLAFRLAKVHPTCSYTYGYKKSTVLVSLLNAVILLVAVGII
                     IAESIGKLLHPRPVEGDAIAWVAGIGVVINALTAWLFMKDKDKDLNVKGAYLHMAADAL
                     VSVGVVVSGIVIAHTGWYIIDPIIGLLIAFVIIVSTWGLLRDSIRLSLDGVPAGTDIDK
                     VEKTIHSVEGVEGVHHLHIWAISTTETALTAHVSVADISEMERIKQEIKHDLQHVGIQH
                     ATLEFETSGFKCDGRCCSD"
     CDS             4521..5087
                     /locus_tag="ctg146_4"
                     /transl_table=11
                     /translation="MEPIINSQAPEFKVQAYHNGSFKTVSSDDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYDQFQAMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGV
                     LSRAFGVMIEEDGMAYRGTFLVNPEGKIKLAEINDNGIGRNADELLRKVEAAQFIAEHP
                     SEVCPAKWKKGGETLKPSIDLVGKI"
     CDS             5181..5354
                     /locus_tag="ctg146_5"
                     /transl_table=11
                     /translation="MLDSSMRNQLSGIFSGLTNEYIFDIQVSPEHESRKELLELLNDVA
                     SCSDKITVQVADG"
ORIGIN
        1 ggtattgtgt actccccttg acggctctat ctttgcaaca tagaaaaaag taacactatt
       61 agtataatcg ctatatcaaa accttttgct aattatgtaa gcatataaga tgattaaacg
      121 tgtgtcggaa attttttatt ggttgtttcc ggcataaaaa agggactctc ctgcgaagga
      181 tggttccttt tttatttttt tgatattatt tataatcctt cctgtttgat acctatatca
      241 ggcgatacgc tgcactatat ctaccgtttt agtaactatt tgtagggatt ttggccgttg
      301 ggaatacatg atgtttttgt tgattgtttc gcatgccata taaataacgt acctttgcac
      361 cgaaattaaa accgaagaaa acaaaataag gtatgaagtt tgaaaacgag aactgggcgc
      421 agttcaaagg cgaaatttgg aaaagagaga ttaacgtaag ggattttatc cagaacaact
      481 acaaacctta cgaaggggac gactctttct tgaaagactc ctcagataag acgaagaaag
      541 tttgggataa attgaccgaa atgtttaagg ttgaaaggga gaagggtgtt tacgacgctg
      601 aaacaaaata tcctcaaggt atagatactt acggaccagg ttatattgac aaagaaaacg
      661 aagtaattgt cggcctgcaa accgatgccc ccctgaaacg cggtatttac ccgaaatgtg
      721 gtacccgcat ggtggaaaac agtctggaag catatggcta caaactggac ccgatgacaa
      781 aagaaatctt cacgaaatac agaaagacgc ataacgaagg cgtattctcc gcttatacag
      841 atgaaatgct tgcagcgcgc cgttccgcta tcatcaccgg cctgccggat gcttacggac
      901 gcggacgtat tatcggtgac taccgccgtg tagcacttta cggtactgaa atcctgatcg
      961 ctgaaaagaa acattatctg aaaagactcg atattcagga actgaccgaa gagatcatcc
     1021 agagccgtga ggaaatatcc gaacagatca aggcgctgaa ggctttcgaa aagatgtgtg
     1081 ccggttatgg tttcgacgtg accaagcctg caaaggatgc acgcgaagcg gttcagtttg
     1141 tctatctggg ttatctggct gcggtgaagg accaggatgg ggctgccatg tcgttgggac
     1201 gtacttctac tttcctcgat atttatattg aaaaagatat ccgcgaaggc aagctgacgg
     1261 aagaagaagc tcaggagctg atcgatcagt ttattatcaa actgcgtatc gtccgtttcc
     1321 ttcgtactcc ggaatataac gacctgttct ccggtgaccc tgtttgggta accgagtctt
     1381 tgggcggaca gggtgtcgac ggccgttcat tggtgacgaa gacgtcttac cgttatctgc
     1441 atacattata taacctcggc ccggcaccgg aacccaactt aacggtactg tggttcaaca
     1501 acgctcctga aaactggaaa cgtttctgtg cgaaggtttc tatcgatact tccgctatcc
     1561 agtatgagaa cgacgatctg atgcgtccgg attatggaga tgattatggt attgcctgct
     1621 gcgtgtctcc gatgaagatc ggtaagcaga tgcagtattt cggcgcacgt gccaacctgg
     1681 ctaaatgttt gctgtatgcc atcaacggcg ggcgcgacga aagatcaggt gtacaggtgg
     1741 ctccgaggtt tgaaccgatc accagcgaat acctggatta caacgaagtg atggagaaat
     1801 acgaacagat gatgcgctgg ctggctaaag tttatgtgaa tgcactgaag atcatccatt
     1861 acatgcacga taaatatgcg tatgaagcat tcgagatggc tttgcatgac ggttcggtag
     1921 aacgtatccg cgcaacaggt attgccggcc tttctatcgt tgccgactca ttggctgcta
     1981 tccgcgatac gaaagtaaaa gtgatccgcg acgaacgtgg cctggctgtc ggtttcgaac
     2041 gtgaaggaga ttatgttcct ttcggtaaca atgacgatcg tacagatagt attgctgtcg
     2101 acatcactga aaagttcatg gaatatctgc gtcagcacca gacataccgc aacgccactc
     2161 cgacacagtc tatcctgact atcacatcca atgtggtgta tggaaagaag acaggtgcaa
     2221 ctcccgacgg tcgcgaaggt ggtacgccgt ttgctccggg tgcgaacccg atgaacggac
     2281 gtgataccaa aggcgctgtt gctgcactgg cttctgttgc taaactgccg ttccagcatg
     2341 cacacgacgg tatctcttat acattcgctg tttctcctgc aacgttagga aaagaacgtg
     2401 atattcagat cgccaatatg gtcggcctgc tggacggtta ctttactcct gacggcggac
     2461 agcatctgaa tgtgaacgta ttcgacaagg agctgttgct cgatgctatg gagcatccgg
     2521 aaaactatcc gcagttgaca atccgcgtat cgggttatgc agtaaacttt gtgaaactga
     2581 cacgcgaaca gcagctggat gtgatttcaa gaacaatcaa tcattctttg taattgaaag
     2641 ttggaaatta aaggaaacat acattcacta gagagtttcg ggaccgttga tggtcccgga
     2701 attcgttttg ttgtctttat gcagggttgt ccgctccgtt gcctgtattg ccacaatccg
     2761 gatacctggg atagcaaagg tcttgccaaa tatcagatga cgcctgaaga attattggct
     2821 gaggttttac gatataagaa ctttatcgcc aagggaggcg tgactgtcac cgggggagaa
     2881 cctttattgc aagctgaatt tctcagggag tttttccgtc tttgccggga agccggtatt
     2941 catacggcgc tcgatacatc cggctatatc tgcacaccga aagccctggc ggttctggaa
     3001 tatgccgacc tggtcttatt ggatataaaa accatcgatc ccgacctgca tccccgtcta
     3061 acggccgtga aactggataa tacgttacgt ttcctggacg aactggaaaa acgcggtgtt
     3121 ccggtttgga tacgccatgt cattgtgccc ggactgacgg ataatgacga agccttggat
     3181 aaactggccg aatacatcag ccactatacg gttgtacaga aggcggaact cttgccttat
     3241 cacacgatgg gcacatataa atacgaggct caaggtctcg actacaaact gaaagaggta
     3301 gaacctttat cggccgaacg tctggcaaat gccaaagaaa tatttaagaa gcacggtata
     3361 accatctgat aatttaccta taatcaggta tatttttatt gctctaaaaa acatagtttt
     3421 gtcccatatt gttactcaaa taaaagtagg aatgaatatg gaacaacatc atcatgatca
     3481 tcaccaccac gaaataacct ctttgaacaa ggctttcatt ataggtatct ccctgaacct
     3541 ggcttttgtc gtagccgaat ttacggctgg cttctggtat aattcattag gacttctgtc
     3601 ggatgcaggg cataacctgg gagatgtggc cagcctggta ctggctatgc tggctttccg
     3661 gctggctaag gtgcacccga cttgcagcta tacctatgga tataagaaaa gcactgtact
     3721 cgtatccctc ctgaatgcgg tcatcctctt ggtggccgtc gggattatta ttgctgaaag
     3781 tataggaaaa ttgctgcatc cgcgtccggt cgagggtgat gcgatcgctt gggtggcagg
     3841 tatcggagtc gttataaatg cacttacggc atggcttttc atgaaggata aggataaaga
     3901 cttgaatgtg aaaggtgctt acttgcatat ggctgccgat gcactggtat cggtcggcgt
     3961 ggttgtctcc ggaattgtta ttgcccatac cggatggtat atcatcgacc cgattatcgg
     4021 cctgttgatt gcgttcgtta tcattgtttc tacctgggga ctgctacgcg acagtatccg
     4081 tctctctctg gacggtgtgc cggcgggaac ggacatcgat aaggtggaaa agacgataca
     4141 ttcggtcgaa ggcgttgaag gcgtccatca tctccatatc tgggcgatca gtacgaccga
     4201 aacggcactt accgcccatg tttccgttgc ggatatttca gaaatggaac gtatcaaaca
     4261 ggagataaaa cacgaccttc agcatgtggg aatccagcat gctacgctcg aatttgaaac
     4321 gtcgggattc aagtgcgatg ggcgctgttg ctcagactga aacaggtata ttataaacct
     4381 gatttactga tactcaaaga ttttgtctat gactgcataa aacctttcga ttggattttt
     4441 tgttcttacc tcgaatcggc aacttatctt tgcaccagta acaaaaacaa aatagtaata
     4501 acgattaaaa taaaaattct atggaaccta ttatcaattc acaagcaccc gagtttaaag
     4561 ttcaggcata ccataacggt agtttcaaaa ctgtaagcag cgacgacatc aaaggcaaat
     4621 gggcgatttt ctttttctac ccggctgact ttacatttgt ttgtccgacc gagttggtag
     4681 atatggctga caaatatgac cagttccagg caatgggtgt agaagtttac tctgtaagta
     4741 cagattcaca cttcgtacac aaagcatggc acgatgcttc tgaaacgatt cgtaagatca
     4801 aatacccgat gttggcagac ccgacaggcg tattgagccg tgcattcggt gtaatgatcg
     4861 aagaagacgg tatggcttac cgcggtacat tcctggtaaa tccggaagga aagatcaaac
     4921 tggcagaaat caacgataac ggtatcggcc gtaacgccga cgaactgctt cgtaaagtag
     4981 aagctgccca gttcatcgcc gaacatccga gcgaagtttg ccccgccaaa tggaaaaaag
     5041 gtggcgaaac attgaaaccg agcatcgatc tggtcggtaa gatctaatag taaaactgga
     5101 ggccggcaac atattgtcgg ccttttttat ctaaacaaat atcagttgtt ctattcataa
     5161 ttaaaataaa ggattcaact atgttagatt catcaatgag aaatcagttg tccggtatct
     5221 ttagcggatt gacgaacgaa tatatatttg atatacaagt ttctcctgaa catgaaagca
     5281 ggaaggaact attggaattg ctgaacgatg tggcttcctg ttccgataag atcaccgtac
     5341 aggtggcaga cggcga
//
