LOCUS       c00255_gnlPro..         5296 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GDNKDNGG_255.
ACCESSION   c00255_gnlPro..
VERSION     c00255_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 04:16:26
            Original ID  :: gnl|Prokka|GDNKDNGG_255
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5296
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5296
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[582:5294]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      583..5294
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..5296
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5296
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             3..521
                     /locus_tag="ctg255_1"
                     /transl_table=11
                     /translation="RYRGILDWDTPIHWIDTSEMDFKEEFVGETFGRINKAEADLLLSE
                     LKIYINRIGGSRILEEKIDFGIISPYKAQVQYLRNKIKADASLKPYRSLFTVNTVDGFQ
                     GQERDVIFISLVRANEEGQIGFLNDLRRMNVAITRARMKLVILGEAETLKHHGFYRKLL
                     EFIQNIGNQ"
     CDS             complement(583..1632)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg255_2"
                     /sec_met_domain="IlvN (E-value: 1.5e-46, bitscore: 146.9,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 2.5e-41, bitscore: 130.3,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQLNFGGVIENVMTREEFPLEKAREILKDETIAVIGYGVQGPGQ
                     ACNLRDNGFNVIVGQRPGKTYEKAVADGWVPGXXXTLFGIEEACQKGTIVMCLLSDAAV
                     MSVWPTIKPYLTPGKALYFSHGFAITWNDRTGVVPPKDIDVIMVAPKGSGTSLRTMFLE
                     GRGLNSSYAVYQDATGKAFDKTIALGIGIGSGYLFETTFIREATSDLTGERGSLMGAIQ
                     GLLLAQYEVLRENGHTPSEAFNETVEELTQSLMPLFAKNGMDWMYANCSTTAQRGALDW
                     MTPFHDAIKPVVQKLYASVKSGNEAQISIDSNSKPDYREKLNAELKALRESEMWQTAVT
                     VRKLRPENN"
     CDS             complement(1683..2435)
                     /locus_tag="ctg255_3"
                     /transl_table=11
                     /translation="MMSENNKIGTYKFVAEPFHVDFTGRLTMGVLGNHLLNCAGFHASD
                     RGFGIATLNEDNYTWVLSRLAVELDEMPYQYEDFSIQTWVENVYRLFTDRNFAIIDKEG
                     KKIGYARSVWAMISMNTRKPADLLALHSGSIVDYVCDEPCPIEKPSRIKVASKEPVAAL
                     VAKYSDIDINGHVNSIRYIEHILDLFPIEMYKEKRIRRFEMAYIAESYYGDELTLFMDD
                     AGEGVYDVEVKKNGSEVVCRSKVIFTEK"
     CDS             complement(2432..2992)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg255_4"
                     /sec_met_domain="ALS_ss_C (E-value: 4.2e-20, bitscore:
                     60.8, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKLYTIIVHSENFAGLLNQVTAVFTRRQINIESLNVSASSIKG
                     VHKYTITAWTDKDTIEKVTKQITKKIDVLQAHYFTDDEIYQHEIALYKITTPTLEEKPE
                     VSKVIRKYNARIVEVNAVFAIVEKNGMSEEITNLYEELRQLDCVLQFVRSGRVAITTSC
                     FERVNEYLADREAKYRQSKHQES"
     CDS             complement(3025..4776)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg255_5"
                     /sec_met_domain="TPP_enzyme_C (E-value: 5.9e-48, bitscore:
                     151.4, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADNNKKSNFSSLSQGEGRGEALITGAEALMRSLEHQGVKTIFGY
                     PGGSIMPVFDALYDHRKELNHILVRHEQGATHAVQGFARVSGEVGVCLVTSGPGATNTI
                     TGIADAMIDSTPIVVIAGQVGTNFLGTDAFQEVDLVGITQPITKWSYQIRRAEDVAWAV
                     ARAFYVAKSGRPGPVVLDFAKNAQVEKTEYAPTKVDYVRSYLPVPEMEPEAIRKAAELI
                     NGAERPLVLVGQGVELGEAQQELRAFIEKAGMPAGCTLLGLSALPTNHPLNKGMLGMHG
                     NLGPNINTNKCDVLIAVGMRFDDRVTGKLETYAKQAKIIHFDIDPAEIDKNVKTDVAVL
                     GDCKETLAAVTQLLEPANHQEWIDSFRQYEEVEEEKVIRPELHPAGKALSMGEVVRAVS
                     EATHNEAILVTDVGQNQMMSARYFKYSKERSIVTSGGLGTMGFGLPAAIGATFGRPDRT
                     VCVFMGDGGLQMNLQELGTIMEQKAPVKMILLNNNFLGNVRQWQAMFFNRRYSFTPMMN
                     PDYMKIASAYDIPARRVMTREELAKAIDEMIATDGPFLLEACVEEEGNVMPMTPPGGSV
                     NQMLLEC"
     CDS             complement(4868..5098)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg255_6"
                     /sec_met_domain="ILVD_EDD (E-value: 1.4e-16, bitscore:
                     49.0, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFLPKRLPXXXXXXIKDGDIIEIDIPNRSINVKLTDEELAARLMT
                     PVTRNREVSKALKAYASMVSSADKGAVRLID"
     CDS             complement(5052..5294)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg255_7"
                     /sec_met_domain="ILVD_EDD (E-value: 4.1e-35, bitscore:
                     110.1, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="CDGILGGKVVSGDVVVITHEGPKGGPGMQEMLYPTSYIKSRHLGK
                     ECALITDGRFSGGTSGLSIGHVSPEAAAXXXXXXD"
ORIGIN
        1 tgcgttaccg gggcatcctg gactgggata cccctatcca ctggattgac acctccgaaa
       61 tggatttcaa agaagaattt gtgggagaga ctttcggacg catcaataaa gcggaagcgg
      121 acctgctcct atcagaactg aaaatctaca tcaaccgtat cggtggcagc cgcattctgg
      181 aagaaaaaat agacttcggc atcatctccc cttacaaggc acaagtgcaa tatctccgca
      241 acaagataaa ggcagacgct tcactcaagc cttatcgcag cttgttcact gtaaatacgg
      301 tggatggctt ccaagggcaa gagcgcgatg ttatcttcat cagccttgtc cgcgccaacg
      361 aagaagggca gataggattc ctgaatgatt taaggagaat gaatgtagcc atcacccggg
      421 cacgtatgaa gctggtgata ttgggagagg cagagacgct gaaacatcac ggtttttacc
      481 ggaagctatt ggagtttatt caaaatatag ggaatcaata ataaagaaat accccctccg
      541 gtaacaccga agagggtatt tcacctattt atataaagct gattaattgt tttccggacg
      601 aagtttacga actgtaacag ctgtctgcca catttcgctt tcacgcaagg ctttcaattc
      661 tgcattcagc ttctcacgat aatcgggttt ggagttgctg tcgatagaaa tctgagcttc
      721 gttacccgac ttcacgcttg catacaattt ctgtacaacc ggcttgatgg catcatggaa
      781 cggggtcatc caatccagcg caccacgctg tgccgtagta gaacagttgg catacatcca
      841 gtccatacca ttcttggcaa acaatggcat caaagattga gtcaactctt ccacagtttc
      901 attgaatgct tcagaaggag tgtgaccgtt ttcgcgcaat acttcatact gagccaacag
      961 caatccctgg atagcaccca tcaatgaacc gcgttcaccg gtcaagtcgg aagtggcttc
     1021 gcggatgaaa gttgtctcga acaaatatcc cgaaccgata ccgataccca gtgcaatcgt
     1081 cttgtcaaaa gccttacccg tagcatcctg atagacggca tatgatgagt tcaagccacg
     1141 cccttcgagg aacatggtac gaagtgaagt accggaacct ttgggagcaa ccataataac
     1201 atcaatatct ttaggaggaa ccacgccggt acggtcgttc caggtaatag caaaaccatg
     1261 agagaagtaa agagcctttc cgggagtcaa ataaggtttg atagtcggcc atacagacat
     1321 tacagctgcg tcagacaaca gacacatgac gatagtacct ttctgacaag cctcttcgat
     1381 gccgaacaaa gtnnnnnnnn nncccggtac ccatccgtca gccacagcct tttcgtatgt
     1441 cttacccgga cgttgtccta caatcacatt gaaaccgtta tcacgcaagt tgcaagcctg
     1501 gcccgggcct tgtacgccat aaccgattac tgcaattgtt tcatctttca aaatctcacg
     1561 tgctttttcc aaaggaaatt cttcgcgggt cattacattc tcaataacac cgccaaaatt
     1621 caattgtgcc attttttgtt ttaattttta tttgtggctt acgccaacgt tgataaattt
     1681 atttatttct ctgtaaatat cactttagag cgacagacca cttcgctgcc attctttttc
     1741 acctccacat cgtaaacgcc ctcaccggca tcatccatga acaaagtcaa ctcatcccca
     1801 taatagcttt cggctatata tgccatttca aaacgacgga tgcgcttctc cttatacatc
     1861 tctatcggga acaaatcaag gatatgctcg atgtaacgga tgctgttcac atgcccgtta
     1921 atgtcaatat cactgtattt ggctaccaaa gcagcaaccg gttcctttga ggccaccttt
     1981 atccgcgaag gtttttctat cgggcaaggc tcatcgcaaa cataatctac aatgctgcca
     2041 ctatgaagag ccagcaaatc tgccggctta cgggtattca tgctaatcat cgcccaaacg
     2101 gaacgtgcat acccaatctt cttaccctcc ttatcgatga tagcgaaatt acggtctgta
     2161 aaaaggcgat agacattctc cacccaagtc tgaatggaga aatcttcgta ctgataaggc
     2221 atttcatcca gttcgacagc cagacgggac aatacccaag tataattgtc ctcgtttaga
     2281 gtcgcgatgc caaaaccgcg gtcgctggca tggaaacccg cacaattcaa caaatggttg
     2341 cccaatactc ccattgtcag ccgtccggta aagtcgacat gaaacggttc ggcaacaaac
     2401 ttgtaggtac ctatcttatt gttctcgctc atcatgattc ctggtgttta ctttgacgat
     2461 attttgcttc acggtccgcc agatattcat tgacacgctc aaagcagctg gtcgtaatgg
     2521 ctacgcgacc cgaacggacg aactgaagta cgcaatccaa ctggcgcaac tcctcataaa
     2581 gattggttat ttcctcactc atgccattct tctcaacaat ggcaaataca gcattcacct
     2641 ccacaatacg ggcattgtac ttacggatta ctttggacac ctccggtttt tcttccagcg
     2701 taggggtcgt tattttataa agggcaatct cgtgctgata gatttcatca tccgtgaaat
     2761 aatgggcttg cagcacatct attttcttgg taatctgctt ggtcaccttt tcaatggtat
     2821 ccttgtccgt ccaagcggta atggtatatt tatgtacccc cttgatggag gaggccgata
     2881 cattcaaact ctcgatgttt atctgccggc gggtaaacac agccgtcacc tgattcagca
     2941 aaccggcaaa gttttccgaa tgaacaatga ttgtatatag ttttttatcc atagatagag
     3001 taagttcttt aatttcgcat gacttcagca ttccaacaac atctgattga ccgaaccgcc
     3061 cggaggtgtc atcggcatta catttccttc ttcctccaca caagcttcga gcaggaacgg
     3121 tccgtcagtc gctatcatct cgtcaatcgc ttttgccagc tcctcgcggg tcattaccct
     3181 gcgtgcagga atatcatatg ccgaagcaat cttcatataa tccggattca tcatcggagt
     3241 aaacgaataa cgacgattga agaacatcgc ctgccattga cgaacattgc ccaggaagtt
     3301 attgttcaac agaatcatct tcaccggagc cttttgttcc atgatggtac ccagttcctg
     3361 caggttcatc tgcaagccgc cgtctcccat gaatacgcat accgtgcggt ccggacgtcc
     3421 aaaagtggca ccgatagctg ccggaaggcc gaagcccata gtacccaggc caccggaagt
     3481 aacaatgcta cgttccttgc tgtatttgaa ataacgggcg gacatcatct gattctggcc
     3541 gacatcagtc accaatatcg cctcgttatg ggtagcctca ctcacagcac gcaccacctc
     3601 acccatgctt agcgcttttc ctgccggatg taattccgga cggattacct tttcctcttc
     3661 cacttcctcg tattgccgga aactgtcaat ccattcctga tggttggcgg gttccagcaa
     3721 ttgggtaacg gcagccagtg tctctttgca atctcccagg acagcaacat cggtctttac
     3781 attcttatct atttcggcag ggtcaatatc aaagtggata atcttcgcct gcttggcata
     3841 tgtttccaat ttacccgtca cacggtcatc aaagcgcatg cctacggcga taaggacatc
     3901 acatttattg gtattgatat tgggccccag gttaccgtgc ataccaagca ttcctttgtt
     3961 caacggatgg ttcgtaggta atgcggaaag tcccaataaa gtgcatccgg caggcattcc
     4021 ggctttctca atgaaagcac gaagctcttg ctgggcttct cccagctcca cgccttgtcc
     4081 taccagcacc aaaggccgtt ccgcaccatt tattaattca gcagccttcc gtatggcctc
     4141 cggctccatt tcgggaacgg gaagatagct acggacatag tccaccttag taggcgcata
     4201 ttctgttttc tccacttggg cgtttttagc aaaatccagc acgaccggtc ccggacgtcc
     4261 gctctttgcc acatagaaag cacgtgccac tgcccaggcc acatcttctg cacgacgaat
     4321 ctggtaactc catttcgtga taggctgagt aatgcctacc aagtccactt cctgaaatgc
     4381 atccgtaccc aagaagttcg tccctacctg gccggcaatg acaacgatag gcgtactgtc
     4441 aatcatggca tctgcaatgc ccgtgatagt attggtagca cccggtccac tggtaaccag
     4501 acaaacgcca acctcgcctg atacacgtgc aaagccttgt accgcatggg ttgctccttg
     4561 ttcatggcga accaagatgt ggttcaattc cttccggtgg tcgtataagg catcgaatac
     4621 cggcatgatg ctccctcccg gataaccgaa aatagtcttt actccctgat gctccagaga
     4681 gcgcatcaat gcttctgcgc ctgttattag agcctcaccc cgaccctctc cttgggagag
     4741 ggaggaaaag ttactctttt tattattatc agccatactt tatctattca ttatatttaa
     4801 aagctcaata gtagcttgaa aaatttgttt cattctctcc tcccctcgct ttcggaaagg
     4861 ggggctttta atcaatcagc ctcacagccc ctttgtcagc ggaactcacc atactggcat
     4921 atgctttcaa tgctttggag acttcacggt tgcgggttac cggagtcata aggcgtgctg
     4981 caagctcttc atccgtcaac ttcacattga tagaacggtt cggtatatca atttcgataa
     5041 tgtccccatc cttaatctnn nnnnnnnnnn nnnncggcag ccgcttcggg agaaacatgt
     5101 ccgatactta gtccggaagt accaccactg aaacgcccgt cggtaatcaa tgcacactcc
     5161 ttacccagat ggcgggattt gatataagaa gtaggataaa gcatctcttg catacccggt
     5221 ccacctttcg gcccttcgtg agtgatgacg acgacgtctc cactgaccac ctttccaccc
     5281 agaataccgt cacaag
//
