LOCUS       c00189_gnlPro..         5947 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OPPKNDCE_189.
ACCESSION   c00189_gnlPro..
VERSION     c00189_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 03:25:12
            Original ID  :: gnl|Prokka|OPPKNDCE_189
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5947
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..612)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: ECH_1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg189_1"
                     /sec_met_domain="ECH_1 (E-value: 1.6e-65, bitscore: 209.3,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METKREWTTIKEYEEILFDFYNGIARITINRERYRNAFTPTTTTE
                     MSDAFYICRERPDINVVVLTGAGDTAFCAGGDMNVKGRGGYVGKDGVPRLNVLDVQKQI
                     RSLPKPVIAAVNGFAIGGGHVLHVVCDLTIASENAIFGQTGPRVGSFDAGFGSSYLARV
                     VGQKKAREIWFLCRKYNAREALDMGLVNKVVPLDKLEDEYV"
     protocluster    1..5947
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:4800]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..4800
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..5947
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /kind="single"
                     /product="TPP_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5947
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_fatty_acids"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /tool="gutsmash"
     CDS             complement(616..2274)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg189_2"
                     /sec_met_domain="TPP_enzyme_C (E-value: 3.5e-08, bitscore:
                     21.8, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LVALLIEHGVSNIVLCPGSRNSPIVHTLANHSFFNCHSVTDERSA
                     GFFAVGLALHGGKPAAVCCTSGTALLNIHPAVAEAFYQKVPLVVISADRPAAWIGQMDG
                     QTLPQPWVFGSLVKKSVQLPEIHTDQDEWYCNRLINEALLELNHHGKGPVHINVPISEP
                     LFQFTTPELPKVRVITRYQGLNVYDRKYDDLIERLNKYSKRMMIAGQMSLIYLFEKKIC
                     KLLYKHFTWLSEHIGNRIVPGLPIKNFDAILYATPEEEKEKLVPELVITYGGHIVSKRL
                     KEFLRKHPPKEHWHVSLDGEVADLFGSLTTVIEMDPFEFLEKIAYMLENRQIEYPKAWE
                     YKSQNLPEPEFAYSEMVAIGDLIRSLPAESALHLGNSSTVRYAQLYTVPETVEVCCNRG
                     TSGIEGSLSTAIGYASASQKLNFIVIGDLSFFYDMNALWNGNLHNNLRILLLNNGGGEI
                     FQALPGFKMNERTHRYVTASHQTSAEGWATERGFSYSAVHNAEELAAAMSAFTQTEQPS
                     EHPLFMEVFTDKAEDVRLLKEYYHQLKNSSQAQNV"
     CDS             complement(2474..3571)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Chorismate_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg189_3"
                     /sec_met_domain="Chorismate_bind (E-value: 4e-56, bitscore:
                     178.8, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIPRDSYEIIDTLIKQGRSFAIWRIPGEDRLHFRMQSAGSPCLLY
                     DIKDLNERSGFVIAPFQVTAERPIVLIQPDCFEIPSESDWDFSREKNEFPSNEIEIPSE
                     SEEKERYAYHFSLFTNPLLKGSQDKLVLSRSKTIRKDPSFSPGKAFFAAEKRYIRSYVY
                     LCHTPETGTWMGGTPEILLSGEKGDWQTVALAGTQSLRDGKLPKSWDHKNWREQQLVAS
                     YIRRQLSTLGITPEEKGPYSARAGEVSHLKSDFFFSLPDSEKLGDVLQLLHPTPAVCGL
                     PKEEAYHFIIEHEGYDRSYYSGFIGWLDPKGKTDLYVNLRCMNILPQTFTLYAGGGLLA
                     ASQLEDEWQETEDKLDTMRRIVGNL"
     CDS             complement(3568..4800)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: HBT"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg189_4"
                     /sec_met_domain="HBT (E-value: 7.1e-18, bitscore: 53.4,
                     seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYKLLVLDVDGTLLNDKKEITPRTHAALLKAQQMGVHVVLASGR
                     PTNGVQPLAETLELNHYGGFILSYNGGQIINAQTGELMFEKRIDPAMIPYLNRKAKENG
                     FAIFTYHKDYILTDSPENKHVQEEAELNKMRIIGVENFPEAVDFAPCKCILTSDDENNL
                     VGLENHWKKRLDGVLEAFRSEDYFLEVAPHFINKGNTLAVLMEMLNITTEEVVAIGDGV
                     ADVSMLQLAGTGVAMGNARDSVKACADFTTLPNNMEGVATAIEKAILATIKPTEVPLDQ
                     LNARAKHALMGNLGIQYTYASEDRVEATMPVDERTRQPFGILHGGATLALAETVAGLGS
                     MILAKPDEMVVGMQVSGSHMSSAHEGDTVRAVGTIIHKGRSSHVWNVDIFTSTDKLVSS
                     VRVINSILKKK"
     CDS             complement(5059..5865)
                     /locus_tag="ctg189_5"
                     /transl_table=11
                     /translation="MNYKLLVLDLDGTLTNSKKEITPFTRETLLQAQEKGLHLVLASGR
                     PTYGIVPLAEELNMKQYGGFILSFNGGKVIDLMTDKVLFEQALPPDIVPVLYNRSKEAG
                     LAILSYNGKYILSEHPENKYVQYESFLTKMKIKATDDFLRDLHQPADKCLVVGEPEMLV
                     PLEEKLRQELGKRINVYRSEAFYLELVPKGIDKAASLSRLLERTRIKQEEVIAIGNGFN
                     DVSMIHFAGLGVAMANAQPPVKANADRITQYTNDEDGVARFIQELL"
ORIGIN
        1 aacgtactcg tcctctaatt tatccaaggg gactacctta tttaccagac ccatatccaa
       61 agcctcccgc gcattgtact tgcggcatag gaaccagatc tcacgggcct ttttctgccc
      121 gaccacacgg gcgagataag aagagccgaa cccggcgtcg aagctaccca cacgagggcc
      181 tgtctgtccg aagatcgcgt tctcggaagc gatcgtaagg tcgcaaacca cgtgcaatac
      241 gtgtccgccg ccgatagcga aaccattcac ggccgcgatg acgggtttcg ggagagagcg
      301 gatctgcttt tgtacgtcca atacgttcaa gcgaggtacg ccgtctttac ccacgtagcc
      361 gccacgccct ttcacgttca tatcgcctcc tgcgcaaaac gccgtatctc cggctcctgt
      421 cagaacgact acgttaatat ccggacgttc ccggcagatg taaaaggcgt cgctcatctc
      481 cgtggtcgta gtcggggtaa aagcgttgcg ataccgttcc cggttgatcg taatgcgggc
      541 gatgccgttg tagaaatcaa ataagatctc ctcgtattcc ttgatggttg tccattctct
      601 tttcgtttcc atatattata cgttttgtgc ttgggatgaa ttttttagtt gatgataata
      661 ttccttgagc aatcgtacgt cttccgcttt gtccgtgaaa acctccatga ataggggatg
      721 ttcgctcggt tgctccgttt gggtaaaggc gctcatcgcc gctgccaact cctcggcatt
      781 atgaacggcg gagtaggaga atcctctttc cgtggcccat ccctccgctg aggtctggtg
      841 ggaggcggtg acgtaacggt gcgtacgctc gttcatcttg aatcccggta aggcttggaa
      901 gatctcgcca cctccgttgt tcaagagcag gatgcgaagg ttattgtgca agttaccgtt
      961 ccacaacgca ttcatatcat agaagaaact aagatcgcct ataacgataa agtttagttt
     1021 ttgcgaggcc gaggcgtatc cgatggccgt agacaaggag ccttcgatcc cgctcgtacc
     1081 ccggttacaa caaacctcaa ccgtctcggg cacggtgtac aattgcgcgt aacgtacggt
     1141 ggagctattc cccagatgca aggccgattc agccggaagc gagcgtatca aatcgccgat
     1201 cgccaccatt tcggagtaag cgaactccgg ctccggcagg ttctgggatt tatattccca
     1261 agccttcgga tattcaatct gcctattctc cagcatataa gcgattttct ccagaaactc
     1321 gaaagggtcc atctcgatca ccgtggtcag gctaccgaat aaatccgcta cttctccatc
     1381 caatgagaca tgccaatgct ccttaggcgg gtgtttccgc aaaaactctt tcagccgctt
     1441 ggagacaata tggccgccgt aggtgattac gagctcggga actagcttct ctttttcctc
     1501 ctccggggta gcgtacagga tagcgtcgaa attcttgatc ggtaatcccg gtacgatacg
     1561 gttgccgatg tgctcggaga gccaagtgaa atgcttgtat agtaacttgc atattttctt
     1621 ctcgaagaga tagatcaagc tcatttgtcc cgctatcatc atccgcttgc tatatttgtt
     1681 caagcgctcg atcaggtcat catatttccg gtcgtacacg ttcaagcctt gataacgggt
     1741 gattacccgt accttcggca attcgggtgt cgtgaactga aataaaggct cggagatcgg
     1801 gacgttgata tgaaccggtc ctttgccgtg atggttcagt tccaacaaag cctcgttgat
     1861 caatcgattg caataccatt catcttgatc cgtatgtatt tccggtagct gcaccgattt
     1921 cttcaccagc gagccgaaca cccagggttg cggtaaggtc tgtccgtcca tttgcccgat
     1981 ccatgcggcg gggcggtcgg cggatatcac gacaagcgga actttctgat agaaagcctc
     2041 cgctaccgcc ggatgtatat tcagtaaggc cgtgcccgag gtacagcaaa cagccgccgg
     2101 cttacctccg tgcaacgcta gccctacggc gaagaatccc gcgcttcgtt catccgtcac
     2161 ggaatggcaa ttgaagaacg aatggtttgc caatgtgtgc acaatcggag agttccggct
     2221 tcccggacat agcacgatat tacttacgcc gtgctctatc agcaaggcta ccaattgaag
     2281 gatgttcttt ttatcgctat acatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnncgtgct ctatcagcaa ggctaccaat tgaaggatgt tctttttatc gctatacatt
     2461 atatgatata aaattataaa ttacccacta ttctccgcat tgtatctaat ttatcctctg
     2521 tctcctgcca ctcatcctct agctgcgagg ccgccaacag accgcctccg gcatacagcg
     2581 taaaggtttg ggggaggata ttcatgcaac ggagatttac gtacagatct gttttgcctt
     2641 tgggatccag ccaaccgatg aaaccggagt aatagctacg atcgtacccc tcatgctcga
     2701 tgatgaagtg ataagcttcc tccttgggaa gaccgcaaac ggcgggagta gggtgcaata
     2761 gttgaagcac gtcacccaac ttctcggaat ccggcaagga gaagaagaaa tcgcttttca
     2821 ggtgggaaac ctctccggcc cgggctgaat aaggcccctt ctcctcggga gtaatcccca
     2881 aggtggacag ctggcggcgt atataggagg cgactaattg ctgttctctc caattcttat
     2941 gatcccagct tttcggtaat ttcccgtctc gtaaggattg ggttccggcc aaagctaccg
     3001 tttgccagtc gcctttctcg ccggataaca agatttccgg tgtcccaccc atccaagttc
     3061 cggtttccgg ggtatggcaa aggtagacat aggagcggat atatcgtttt tctgcggcaa
     3121 agaatgcctt gccgggtgag aaagagggat ctttccggat cgttttgcta cgggatagga
     3181 ctagtttatc ttggcttcct tttagcaaag ggttggtaaa cagggagaaa tggtaagcgt
     3241 atcgttcctt ctcctcggat tcggacggga tttcgatttc attcgaaggg aattcgtttt
     3301 tctctcgaga gaaatcccaa tcggattcgg aggggatttc aaagcaatcc ggttggatta
     3361 aaacgatagg tcgttccgct gtcacctgaa acggagcgat tacaaaaccg ctccgttcat
     3421 ttaagtcttt tatgtcgtat aataggcaag gagagccggc gctttgcatc ctgaaatgga
     3481 gcctatcctc accgggaata cgccaaatag cgaaactccg gccttgcttg atcaacgtgt
     3541 ctattatttc gtaagaatct cttggaatca tttcttcttt agtatgctat tgattacccg
     3601 taccgaggaa accagtttgt cggtagaggt gaatatgtct acgttccaca catgggatga
     3661 acggcctttg tggataatcg tcccgacggc acgtaccgtg tctccctcgt gggcggacga
     3721 catatggctg ccgctaactt gcatgccgac taccatctcg tccggtttcg ccagaatcat
     3781 ggaacctagt ccggccacgg tctctgccag agccagtgta gcgcctccgt gcaagatacc
     3841 gaaaggctgg cgggtccgtt cgtctacagg catggtcgct tccacacgat cctctgacgc
     3901 ataggtatat tgaatgccta gattacccat taaagcatgt ttggcccgtg cgtttagttg
     3961 atctaacgga acctctgtgg gtttgatcgt ggcaaggatc gctttctcga tcgctgtcgc
     4021 cacaccttcc atattattgg gtaaggtcgt gaaatcggca caagccttta cggaatcgcg
     4081 tgcgttaccc atcgccacgc cggtaccggc taattgaagc atggatacgt cggctacgcc
     4141 atcaccgata gcgacaacct cttcggtcgt gatattcagc atctccatca ataccgccaa
     4201 cgtatttccc ttattgatga aatgaggagc tacctccaag aaatagtcct cggagcggaa
     4261 agcttccaat accccgtcca gacgtttctt ccaatgattt tcgagaccca ctagattgtt
     4321 ctcgtcatcg ctggtaagaa tacacttaca gggtgcgaaa tccaccgctt ccgggaaatt
     4381 ctccacaccg atgatgcgca tcttattgag ttcggcctcc tcttggacat gcttgttttc
     4441 cggactatcg gttaggatat agtccttgtg atatgtgaag atcgcgaaac cgttttcctt
     4501 cgccttccgg tttaaataag gaatcatggc cggatcgatt cgtttctcga acatcaattc
     4561 tccggtctgg gcattgatga tctgacctcc gttataagat aatatgaaac caccgtaatg
     4621 attcaattca agggtctccg ccaagggttg aaccccgttg gtgggtcggc cggatgccaa
     4681 tacaacatga actcccatct gctgagcctt taacagggcg gcatgggtcc gaggagttat
     4741 ctctttcttg tcgttaagca acgtcccatc tacgtctaag actaataatt tgtacttcat
     4801 ggcgtcttat tttatggttt agaatacaaa tgtacataaa atagttgata gttagaagac
     4861 atttctatga aaggaattta cgtatatcgg cgcaagctca cgcctatatc ggtgtagcct
     4921 tgcggcgata tacgtacagg cttatgccga tataggcatt ggcctgtgtc gatactggtg
     4981 taaatgtctc tgaaaaaaca cgtacgtgtt ctcgcgaaaa catgtacgtg tttctcggaa
     5041 aatacgtacg tgtttttttc aaagcaattc ttggatgaaa cgagccactc catcctcatc
     5101 gttcgtgtat tgggtgatgc ggtcggcatt ggccttgaca gggggctggg cgttggccat
     5161 agcgactccc aatccggcga aatggatcat ggatacatcg ttgaaaccat tgcctatagc
     5221 gatcacttcc tcctgtttta tccgggttcg ctctagtaag cgggataatg aggcggcttt
     5281 gtcgatacct ttggggacaa gctccagata gaatgcctcg gaacgatata cgttgatacg
     5341 tttccctaat tcttggcgga gcttctcttc caacggtacc aacatctccg gttcgcctac
     5401 caccaagcat ttatcggcag gttgatgtaa atcccgcagg aaatcgtcgg tagccttgat
     5461 cttcatcttt gttaggaatg attcgtattg tacgtattta ttttccgggt gctcgcttaa
     5521 gatatattta ccgttgtagg aaaggatagc cagtcccgct tctttggagc ggttgtataa
     5581 aacaggtacg atatcgggtg gcaaagcctg ttcgaaaagt actttgtctg tcatcaagtc
     5641 gatcacctta ccgccgttga aggaaaggat aaaaccacca tattgcttca tgttcagttc
     5701 ctcggctaaa ggcacgatac cgtaggtggg ccggccggag gctaagacca gatgcaatcc
     5761 tttttcctgc gcttgcagaa gggtttccct cgtaaacggg gtgatctctt ttttactgtt
     5821 tgtcagtgta ccgtctaaat ccagtaccaa taatttatag ttcatgctct tgtttcgtgt
     5881 tgatcgtgat tattttacct ctttgggaga tataattata gagttcttta taaaatgcgt
     5941 gccgggt
//
