LOCUS       c00304_gnlPro..         5836 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FFIEKPEJ_304.
ACCESSION   c00304_gnlPro..
VERSION     c00304_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 09:14:58
            Original ID  :: gnl|Prokka|FFIEKPEJ_304
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5836
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1476
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 112.6; E-value:
                     8.1e-35)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg304_1"
                     /sec_met_domain="FAD_binding_2 (E-value: 7.8e-36, bitscore:
                     112.6, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 2.8e-39,
                     bitscore: 122.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GGAQVSRTFYARCQTGQQLLLGAYSALSRMIGLNKVKMYTRHEML
                     DVVKVDGRARGIIARNLITGQIERFSAHAVVVATGGYVNTFFLSTNAMASNGSAAWQCY
                     KKGAYFANPCMVQIHPTCVPVKGDYQSKLTLMSESLRNDGRIWVPKKLEDAKALQAGTK
                     KGKDIPEADRDYYLERRYPAFGNLVPRDVASRAAKERCDAGFGVNNTGLAVFLDFSDAI
                     NRLGKDVVKAKYGNLFDMYEEITNDDPYETPMMIYPALHYSMGGLWVDYELMTSIPGLF
                     AIGEANFSDHGANRLXXXXXXXXXXXXXNYLSDQIQVPRFSTDLPEFAEAEKGIKDRIK
                     KLMSIKGKESVDSIHKKLGHIMWEHVGMARDAKGLQEAIQMLKELKKEFWSNVYIPGEA
                     DNLNVELDKALRLADFIEIGTLMAHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYV
                     SCWEYQGEDKDPVMLKEDLNYQFVVPQTRNYKK"
     protocluster    1..5836
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2259]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2259
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..5836
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5836
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             1504..2259
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg304_2"
                     /sec_met_domain="Fer2_3 (E-value: 4.1e-27, bitscore: 83.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 1.3e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.4e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDIKVTLKVWRQKSPKDKGQFETYQIDKINQSVSFLEMLDILN
                     ERLVSEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFNDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFVSVNTGGVPDANSIAIPRADAEMAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(2558..2881)
                     /locus_tag="ctg304_3"
                     /transl_table=11
                     /translation="MTRNMEICREFVQLAMENYTKEHSVSYYAKRLNITLQHFCYVIKK
                     VSGRTALDILSNIIIMDAKAQLKSTDMPVKKIAFALGFDNLSFFNKYFRQHVGMTPQEY
                     RES"
     CDS             complement(2878..3426)
                     /locus_tag="ctg304_4"
                     /transl_table=11
                     /translation="MEDNIPKFDLPVDFIVGNSITGEILNQYGRFPCKIKAGIFVLCVQ
                     GTVHATVNLTEFTIRKNDFITLPPGCFIQIHEVSDDVKLCFAGFSSVFVSHINYIKTIT
                     NYLPVIFDSPIMPLPEHISLLYQQAFDLLINAYSMPXXXXXXXXXPSRTRRLSRQSLLS
                     SCKGLLNYTGIIRHIAMLP"
     CDS             3667..4881
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg304_5"
                     /sec_met_domain="Radical_SAM (E-value: 8.4e-15, bitscore:
                     44.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTKRTKSSFRTMSTNRKLESVFLFVTGRCNAKCKMCFYAEDMDK
                     KEKDLTFEEIKTISETAGQFNRLWVSGGEPTLREELPEIIEMFYKNNEIKDVNIPTNGL
                     KPDRIVAWVKQIRQNCPELNINLSLSLDGFGDTHDIQRGVPGNFYKAMGTLKLIEKNFA
                     DDGRVLKNLATVITKYNENEVEDLMIWMLGRFHLSNHIIEAARGMTRDDGVKVLTEPTL
                     RALQDKVVPIYNVYGERMAEETGGFRKPIARFFYLGFIRTLYNVRATNIDHPTPWGMDC
                     TAGETTLVIDYDGRFRACELREPLGNVKDYDCNIAKIMESDAMKQEIAAIGHGDKANCW
                     CTHGCWITSSLVFNPRKMITQVYKGYREMKKLNRPLNISEELLQSIERKYALDEQKLVE
                     LGVRN"
     CDS             5059..5643
                     /locus_tag="ctg304_6"
                     /transl_table=11
                     /translation="MTTKLKDLFDTFLNIAASSQKLGINLIRVAILIIFVWIGGLKFYN
                     YEAEGIVPFVANSPFMSFFYAKSAPEYKEYKLKEGEFNEAKHQWHVENNTYIFSHGLGI
                     MIMAIGILTFLGIFSPKIGLIGSGLAIIMTLGTLSFLVTTPEVWVPDLGSGEHGFPLLT
                     GAGRLVIKDTAIIAGAIVVLSDCAQRILKKK"
ORIGIN
        1 ggtggtgctc aggtatcccg tacgttctat gcccgctgtc agaccggaca gcagttgttg
       61 ctgggtgctt actctgcttt gagccgtatg atcggtttga acaaagtaaa gatgtatacc
      121 cgccacgaaa tgctggacgt tgtgaaggtg gatggccgtg cccgcggtat catcgcacgt
      181 aacctgatca caggtcagat cgaacgtttt tctgctcacg cagtagtggt ggcaacaggt
      241 ggttatgtaa atactttctt cctgtctact aacgcaatgg cttccaacgg ttctgctgca
      301 tggcagtgct acaagaaagg tgcttacttc gctaaccctt gtatggtgca gatccacccg
      361 acttgtgtac cggtgaaagg tgactaccag tctaagctga ctttgatgtc tgaatcattg
      421 cgtaacgacg gtcgtatctg ggttccgaag aaactggaag atgcaaaagc attacaggcc
      481 ggaactaaga aagggaaaga cattcctgaa gcagaccgcg actactatct ggaacgccgt
      541 tatccggcat tcggtaacct ggttccgcgt gacgtggctt cacgtgccgc taaagaacgt
      601 tgcgacgccg gcttcggtgt gaacaacaca ggtctggccg tgttcctcga cttctccgac
      661 gccatcaacc gtctgggtaa agatgttgta aaagctaaat atggtaactt gttcgacatg
      721 tacgaagaaa ttaccaacga cgatccatac gaaactccga tgatgattta tcctgcattg
      781 cactactcga tgggtggtct gtgggttgac tatgaattga tgacttctat tccgggtctg
      841 ttcgctatcg gtgaagctaa cttctctgac cacggagcta accgtctggn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnagaac tatctgtcag accagatcca ggttccacgt
      961 ttcagcaccg acctgcctga gtttgcagaa gctgaaaagg ggatcaaaga ccgcatcaag
     1021 aaattgatga gtatcaaggg taaagagtcg gtagattcta tccataagaa actgggtcat
     1081 atcatgtggg aacatgtagg tatggcacgt gatgcaaaag gtttgcagga agcgatccag
     1141 atgttgaaag agctgaagaa agagttctgg agtaatgtat atattccggg tgaagccgat
     1201 aacctgaacg tagagttgga taaggcattg cgtttggctg actttattga gattggtaca
     1261 ttgatggcac acgatgcatt ggaccgtgcc gaatcttgtg gaggacactt ccgtacagag
     1321 caccagaccg aggaaggcga agccctgcgt catgacgata agttcatgta cgtatcttgt
     1381 tgggaatacc agggtgaaga caaagatccg gttatgctga aggaagattt gaactatcag
     1441 ttcgttgttc ctcaaacacg taactacaag aagtaataat catcaatcac aaaaattaga
     1501 atcatggata aagatataaa agtaacactt aaagtttggc gtcagaaaag tccgaaagat
     1561 aaaggacaat ttgagactta ccaaatagac aaaatcaatc agagtgtttc tttcctggaa
     1621 atgttagaca ttttgaatga acgtttggtc agtgaaggta aagagccgat cgtattcgat
     1681 cacgactgtc gtgaaggtat ctgcggtatg tgttctttgt atgtaaacgg acatccgcac
     1741 ggacctgcaa ccggcgcaac tacttgccag ctttatatgc gtcgcttcaa tgatggcgaa
     1801 actattacaa tcgaaccgtg gcgttctgcc ggtttcccgg taatccgcga cttgatggta
     1861 gaccgttacg cttacgataa aattatccag gcaggtggtt tcgtatcagt gaacacaggt
     1921 ggtgtacctg atgccaattc aatcgctatt cctcgtgcag atgctgaaat ggctatggat
     1981 gctgctgcat gtatcggttg cggtgcttgt gctgctgctt gtaagaacgg ttcggctatg
     2041 ttgttcgttt ctgcaaaggt tagccagttg gctctgttgc ctcagggtaa ggtagaagct
     2101 gctcgtcgtg cgaaagcaat ggttgccaag atggacgaac tgggcttcgg taactgtacc
     2161 aacacgcgtg cttgcgaaat ggaatgtccg aagaacatct ctgtcagcaa catcgctcgt
     2221 ttgaaccgcg agttcatttg tgctaaattg caagactaat atacattata tatgtatgtc
     2281 cggagaggcg ttgcttatgg cagcgcctct tcttttttgc ccctatttta acatttctga
     2341 agtcatagat aaatatcttg aatgttgtta gtgtttctta tatgctatac aacactaatt
     2401 agcattttaa ctattgacaa gttggagaat acctgtatct ttgtactgtg gttttttcat
     2461 aatagtatta gatttaaggt taacaaagtt ggttagggct tgtcgtgaga caggctttaa
     2521 ttgtttatag gggtttgcgt ccggcatatc ccttttttta gctctccctg tattcctgcg
     2581 gggtcattcc tacatgttgc cggaaatatt tgttgaagaa cgaaaggttg tcgaaaccca
     2641 gggcgaatgc aattttcttg accggcatat cggtcgattt aagctgcgct ttggcatcca
     2701 taatgatgat attgctcagg atatccaatg cagtccgtcc gctgaccttt tttatcacat
     2761 aacagaaatg ctgcaaggtg atgttgagcc ttttggcgta ataagacacg ctatgctctt
     2821 tcgtgtagtt ttccatcgcc agttgtacga actcccggca tatttccatg ttacgcgtca
     2881 tgggagcatt gctatatggc gtatgattcc tgtataattc aataacccct tgcatgaaga
     2941 tagtaaagat tgccttgata atctccttgt tctcgatggt tnnnnnnnnn nnnnnnnnnn
     3001 nnnnntaggc atggaataag cattgatcag caagtcgaaa gcctgttggt agagtaagga
     3061 tatatgttcc ggcagaggca tgatcggact gtcgaatatg acaggcagat agttcgtaat
     3121 cgtctttata taattgatat ggctgacgaa tacggaagaa aagcctgcaa aacacagttt
     3181 tacatcatcg gaaacctcat gtatctgaat gaagcaaccc gggggtaagg tgataaagtc
     3241 gttcttccgg atggtaaact cagttaagtt tacagtcgcg tggactgtcc cttgtacgca
     3301 aagcacgaat atgccagcct tgatcttgca cgggaaacgt ccgtactggt tcagtatttc
     3361 acctgtaata ctgtttccta caataaaatc tacggggagg tcgaatttag gaatattgtc
     3421 ttccatactt ttatatacac ttaattgaat gtacaaagat agtttttttg agacaagtta
     3481 gtcgataatc ttaaatatag aacatcgaat cccacaaatg gagattgttt tgtgctgtat
     3541 aatagttgaa atttgcgaat gttttcaagt cttgctgata gatttgttgg gatggtatat
     3601 atttgtctat ctgctaaata agatatggga gctaatacta atagagaaat ctaatcgata
     3661 ataacaatga gtacgaaaag aacaaagagc tctttcagga caatgagcac aaacagaaaa
     3721 ttagaatctg ttttcctgtt tgtcacagga cggtgtaatg ctaaatgtaa aatgtgtttc
     3781 tatgcagaag acatggataa aaaggagaaa gacctgactt ttgaagaaat caagacaatc
     3841 tccgaaacag ccgggcagtt caaccgtttg tgggtatccg gcggtgaacc gacactgcgt
     3901 gaggagttac ctgaaattat agagatgttc tacaagaata acgagataaa ggacgtgaat
     3961 atccctacca acggattgaa gcctgaccgg atcgtcgcct gggtaaaaca gatccgccag
     4021 aattgtccgg aactgaatat caacctgagc ctttctttgg acggtttcgg tgatacgcat
     4081 gatatacagc gcggcgttcc cggcaatttc tacaaagcga tggggacact gaagctgatt
     4141 gaaaaaaact ttgctgacga cggcagggtg ttgaaaaacc tggccaccgt aatcaccaaa
     4201 tataacgaga atgaggtcga ggatctgatg atatggatgc tgggacgttt ccatctttcc
     4261 aatcatatta ttgaagctgc acgcggtatg acccgcgacg acggagtgaa ggttcttacc
     4321 gagcctactc tacgcgcctt gcaggataag gttgttccta tttataatgt atatggggag
     4381 cgtatggcgg aggaaaccgg tgggttcaga aaacctatcg cccgtttctt ctacctgggt
     4441 ttcatacgta cattatataa tgtacgtgcg accaatatcg atcatccgac accctggggt
     4501 atggattgta cggccggcga aacaacactg gtaatagact atgacggtcg tttccgtgcc
     4561 tgtgaacttc gcgaaccgct cggtaatgtc aaggattatg actgcaatat cgcaaagatt
     4621 atggagagcg atgccatgaa acaagagatt gccgctatcg gtcatggtga caaggctaac
     4681 tgctggtgta cgcatgggtg ttggatcact tcatcgcttg ttttcaatcc ccgcaagatg
     4741 atcacgcagg tctataaagg ataccgggag atgaagaagc tgaaccgtcc tctgaatatc
     4801 agtgaagagc ttttgcagag tatcgaacgc aaatatgcct tggacgaaca gaaactggtg
     4861 gagctgggag ttcgtaatta aaatagttcc gataacggat catttataaa gccggttgac
     4921 gggaaatata cctctgttaa ccggctttat tgtgtttaag ggaataattg tcaaattatc
     4981 cggtagaatt gccatttccc ataggggaaa aatgtcggat ctttgcatca taagttaaac
     5041 tgataaacaa agaaagacat gactactaaa ttaaaagatt tattcgacac atttctgaac
     5101 attgcagctt cttcacagaa actgggtatt aacctgatcc gcgtagctat cctgattata
     5161 ttcgtatgga tcggcgggct gaaattctat aattatgaag cggaagggat cgttcctttt
     5221 gtggcaaaca gtccgtttat gagctttttc tatgcaaaga gtgcaccgga atacaaagaa
     5281 tataagttga aagaaggtga attcaatgaa gccaagcatc agtggcatgt agaaaacaat
     5341 acctatattt tttcacatgg actaggcatt atgatcatgg cgatcggtat cctgactttc
     5401 ctgggtatct tttctccgaa aattggcttg ataggttccg gactggcgat cataatgaca
     5461 ttgggtacgc tttcattcct ggtgacaaca cctgaagtgt gggtgcccga tttgggaagc
     5521 ggagaacatg gtttcccctt actgaccgga gcaggtcggc tggtaataaa agatacggca
     5581 atcatagccg gagcgatcgt ggtattgtcc gactgtgcac aacgtatatt aaagaagaaa
     5641 taggaataaa aagtgtttat tttaacactt tggaagcaaa aaacaaacat attgaacctt
     5701 gttagctttt cttatagtgt atgaaacacc atttagcata aaacctcttg acaggtttgg
     5761 attaagcagt atatttgcat cgtggttttt tcataatagt attagattta aggttaacaa
     5821 agttggttag ggcttg
//
