LOCUS       c00386_gnlPro..         2105 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OKLNEFLE_386.
ACCESSION   c00386_gnlPro..
VERSION     c00386_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 09:09:25
            Original ID  :: gnl|Prokka|OKLNEFLE_386
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2105
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2105
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:1401]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..1401
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2105
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2105
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..605
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg386_1"
                     /sec_met_domain="Gly_radical (E-value: 1e-47, bitscore:
                     148.9, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 1e-22, bitscore:
                     67.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="IVDFKTEGEFPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYRNSL
                     PTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDRNGAVASMLSVAKLSYD
                     DSLDGISYTFSIVPQALGKEERERRVKLVSLLDAYFAATGHHINVNVLERETLLDAMDH
                     PEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFHTR"
     CDS             622..1401
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 53.6;
                     E-value: 9.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg386_2"
                     /sec_met_domain="Radical_SAM (E-value: 2e-32, bitscore:
                     100.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHNPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRRGGFPDCVH"
     CDS             complement(1650..2105)
                     /locus_tag="ctg386_3"
                     /transl_table=11
                     /translation="LIAPFSRLGSASEWSREQWRELVSLLPGRTVLAALEEDRERAASL
                     AEHLNVELAVGAPEALFPVMDGAAAAVAVDGDFPSLCSFRGLPVVTLFSTRLPDVYRPM
                     GTFNRSLYSHQCCSPCFRKECDRDVPCNRHIAVRDVLEALREISEKE"
ORIGIN
        1 tgattgtgga cttcaaaacg gaaggggaat tcccctgtta cgggaacaat gacccgcgcg
       61 tggacgacat agcatgcagc ctggtcagca atttcatgga aaaactgcgc aggctccaca
      121 cttaccgcaa ttccctgccc acccagtcca tcctgacgat cacctccaac gtggtgtacg
      181 gcaaaaagac gggcaacacg ccggacggcc gccgagccgg ggaaccgttc gcccccggag
      241 ccaaccccat gcacggaagg gacaggaacg gagccgtggc ctccatgctc tccgtagcca
      301 agctgtccta tgacgactcc ctggacggca tctcctacac cttctccatc gttcctcagg
      361 ccctgggcaa ggaggaacgt gaacgccgcg tcaagctcgt ctccctgctg gacgcctact
      421 ttgccgctac aggtcaccac attaatgtga acgtactgga acgggaaacg cttctcgacg
      481 ccatggatca cccggaaaaa tacccgcagc ttaccatccg cgtttccggc tatgccgtga
      541 atttcatcaa gctgacccgg gaacagcagc aggaggtcat caaccgcact ttccacaccc
      601 gttaacccgg actgcaagcg gatgccgcag gtttttccac aaaaccataa tccggacgga
      661 gaaagctccg tcaccggttt ggttcattcc gtggaatcct gcggcaccgt ggacggcccc
      721 ggcatccgct tcgtcctttt cctgtccggc tgcagcctgc gctgccgcta ctgccataat
      781 ccggatacct cctacgtgcg gaggggccgg ctcaggagcg cagatgatgt cctcaaggaa
      841 atagcccgtt accgggattt tttgcaggcg gcgggcggcg gagtcaccct ctccggcggg
      901 gatcccctct tccagccgga ttttgccggt gccgtgctga aaggctgccg gaaactgggc
      961 ctgcacacct gcctggacac ctctggccat ctgggcgtga atgcgggcga agaaatgctg
     1021 gcggatacgg acctggtcct gctggatatc aaggcatgga atccgaaacg gtatcgcgcc
     1081 ctgacaggcg gggaactgcg ccccacactg gagtttgcgg aacgcctggc ctccctccgc
     1141 aagcccgtgt ggcttaggta tgtgctggta cctggcgtta cggacaatct ggaagaaatt
     1201 gaggaactct cccgccatgc ggcgcttctg ggcaacgtgg aacgcgtgga cgtgctccct
     1261 ttccaccagc tggggagatt caaatgggat gaattgaagc tggactatac cttgaaggac
     1321 gtcccggaag cgtccgcaga actggcagaa caggtccgcg gcattttccg gcggggaggc
     1381 tttccggatt gcgtgcatta accggatttt cgcgctttgg cgccaccaac cgcagcaaac
     1441 cggacgcagg catccccctc ttccggatga ggggccctct cccctccact gcagaatcca
     1501 tgcaaaaagg aagacgcatt tctggaattg ctccggaata cggcatttcc gcaattcctt
     1561 tcgttatttc atcattcttt acggcaacgg cagcattccc gccgtttttt ccgccaacgc
     1621 cgggggacgg cattccgtcc cttcccagat tattctttct cggaaatttc ccgcaaggct
     1681 tccagtacgt cccgcacggc aatgtggcgg ttgcagggaa catcccggtc acattccttc
     1741 cggaagcagg gagaacagca ctggtggctg taaagggaac ggttgaatgt gcccataggg
     1801 cggtaaacat ccggaagccg ggtggaaaac agagtcacca ccggaagccc ccggaaagaa
     1861 cagagggacg gaaagtcgcc atccacggct actgccgccg cagcgccgtc catgacggga
     1921 aagagagcct ccggcgcacc caccgccagc tccacattca aatgttccgc cagggaggcg
     1981 gcacgctccc ggtcttcttc cagagccgcc agcaccgtcc gtcccggcag aagggaaacc
     2041 agctccctcc actgctccct gctccattca ctggcgctcc ccaggcggga aaacggagcg
     2101 attaa
//
